ccl 185ig a549 eml4 alk cells  (ATCC)


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    ATCC ccl 185ig a549 eml4 alk cells
    Ccl 185ig A549 Eml4 Alk Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ccl 185ig a549 eml4 alk cells  (ATCC)


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    ATCC ccl 185ig a549 eml4 alk cells
    Ccl 185ig A549 Eml4 Alk Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hypotriploid human cell line  (ATCC)


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    ATCC hypotriploid human cell line
    Hypotriploid Human Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human keratinocyte cell line hacat  (ATCC)


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    ATCC human keratinocyte cell line hacat
    Human Keratinocyte Cell Line Hacat, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    eml4 alk fusion nsclc cell line  (ATCC)


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    ATCC eml4 alk fusion nsclc cell line
    Eml4 Alk Fusion Nsclc Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ccl 185ig  (ATCC)


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    ATCC ccl 185ig
    Ccl 185ig, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ccl 185ig cell line  (ATCC)


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    ATCC ccl 185ig cell line
    Ccl 185ig Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ccl 185ig a549 eml4 alk cells  (ATCC)


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    ATCC ccl 185ig a549 eml4 alk cells
    Ccl 185ig A549 Eml4 Alk Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    adenocarcinomic human alveolar basal epithelial cell line  (ATCC)


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    ATCC adenocarcinomic human alveolar basal epithelial cell line
    Determination of influenza A virus replication in cells with knocked-down 14-3-3γ expression. <t>A549</t> cells were transfected with 14-3-3γ siRNA for 24 h and then infected with either influenza A wild-type virus PR8 ( A ) or PR8-NS1/1-98 mutant virus ( B ) at an MOI of 0.001. At the indicated time points, the supernatants of the infected cells were collected for titration of the infectious viral particles with a plaque formation assay. The cells were extracted to analyze the protein expression by immunoblotting with anti-14-3-3γ, anti-influenza A virus NP, and anti-β-tubulin antibodies. The experiments were performed in triplicate and the results were analyzed with Student’s t -test. *, p < 0.05.
    Adenocarcinomic Human Alveolar Basal Epithelial Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Interactome Profiling of N-Terminus-Truncated NS1 Protein of Influenza A Virus Reveals Role of 14-3-3γ in Virus Replication"

    Article Title: Interactome Profiling of N-Terminus-Truncated NS1 Protein of Influenza A Virus Reveals Role of 14-3-3γ in Virus Replication

    Journal: Pathogens

    doi: 10.3390/pathogens11070733

    Determination of influenza A virus replication in cells with knocked-down 14-3-3γ expression. A549 cells were transfected with 14-3-3γ siRNA for 24 h and then infected with either influenza A wild-type virus PR8 ( A ) or PR8-NS1/1-98 mutant virus ( B ) at an MOI of 0.001. At the indicated time points, the supernatants of the infected cells were collected for titration of the infectious viral particles with a plaque formation assay. The cells were extracted to analyze the protein expression by immunoblotting with anti-14-3-3γ, anti-influenza A virus NP, and anti-β-tubulin antibodies. The experiments were performed in triplicate and the results were analyzed with Student’s t -test. *, p < 0.05.
    Figure Legend Snippet: Determination of influenza A virus replication in cells with knocked-down 14-3-3γ expression. A549 cells were transfected with 14-3-3γ siRNA for 24 h and then infected with either influenza A wild-type virus PR8 ( A ) or PR8-NS1/1-98 mutant virus ( B ) at an MOI of 0.001. At the indicated time points, the supernatants of the infected cells were collected for titration of the infectious viral particles with a plaque formation assay. The cells were extracted to analyze the protein expression by immunoblotting with anti-14-3-3γ, anti-influenza A virus NP, and anti-β-tubulin antibodies. The experiments were performed in triplicate and the results were analyzed with Student’s t -test. *, p < 0.05.

    Techniques Used: Expressing, Transfection, Infection, Mutagenesis, Titration, Plaque Formation Assay, Western Blot

    Examination of IFN-β mRNA expression in 14-3-3γ knockdown cells infected with influenza A virus. A549 cells were transfected with 14-3-3γ siRNA for 24 h and then infected with influenza A PR8 wild-type virus at an MOI of 2. At 3, 6, 9, and 12 h post-infection (PI), the total RNA of the infected cells was extracted to determine IFN-β mRNA expression with RT-qPCR. Cell extracts were analyzed for 14-3-3γ, phosphorylated IRF3 (pIRF3), total IRF3, influenza virus NP, and β-tubulin with immunoblotting.
    Figure Legend Snippet: Examination of IFN-β mRNA expression in 14-3-3γ knockdown cells infected with influenza A virus. A549 cells were transfected with 14-3-3γ siRNA for 24 h and then infected with influenza A PR8 wild-type virus at an MOI of 2. At 3, 6, 9, and 12 h post-infection (PI), the total RNA of the infected cells was extracted to determine IFN-β mRNA expression with RT-qPCR. Cell extracts were analyzed for 14-3-3γ, phosphorylated IRF3 (pIRF3), total IRF3, influenza virus NP, and β-tubulin with immunoblotting.

    Techniques Used: Expressing, Infection, Transfection, Quantitative RT-PCR, Western Blot

    Analysis of influenza A viral RNAs in 14-3-3γ knockdown cells. 14-3-3γ expression of A549 cells was knocked down and then the cells were infected with influenza A PR8 wild-type virus for 36 h. Total RNA of the infected cells was extracted to examine the levels of vRNA, cRNA, and viral mRNA, respectively, using RT-qPCR (right panel). The protein expression of 14-3-3γ, influenza A NP, and β-tubulin was assayed with immunoblotting (left panel). The experiments were performed in triplicate and the results were analyzed with Student’s t -test. *, p < 0.05; **, p < 0.01; ***, p < 0.001.
    Figure Legend Snippet: Analysis of influenza A viral RNAs in 14-3-3γ knockdown cells. 14-3-3γ expression of A549 cells was knocked down and then the cells were infected with influenza A PR8 wild-type virus for 36 h. Total RNA of the infected cells was extracted to examine the levels of vRNA, cRNA, and viral mRNA, respectively, using RT-qPCR (right panel). The protein expression of 14-3-3γ, influenza A NP, and β-tubulin was assayed with immunoblotting (left panel). The experiments were performed in triplicate and the results were analyzed with Student’s t -test. *, p < 0.05; **, p < 0.01; ***, p < 0.001.

    Techniques Used: Expressing, Infection, Quantitative RT-PCR, Western Blot

    eml4 alk  (ATCC)


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    ATCC eml4 alk
    Characteristics of the lung cell lines included in the study. AD, adenocarcinoma; ATCC, American Type Culture Collection; NE, normal epithelial; UCSF, University California San Francisco; UTSW, University of Texas Southwestern.
    Eml4 Alk, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Digital multiplexed analysis of circular RNAs in FFPE and fresh non‐small cell lung cancer specimens"

    Article Title: Digital multiplexed analysis of circular RNAs in FFPE and fresh non‐small cell lung cancer specimens

    Journal: Molecular Oncology

    doi: 10.1002/1878-0261.13182

    Characteristics of the lung cell lines included in the study. AD, adenocarcinoma; ATCC, American Type Culture Collection; NE, normal epithelial; UCSF, University California San Francisco; UTSW, University of Texas Southwestern.
    Figure Legend Snippet: Characteristics of the lung cell lines included in the study. AD, adenocarcinoma; ATCC, American Type Culture Collection; NE, normal epithelial; UCSF, University California San Francisco; UTSW, University of Texas Southwestern.

    Techniques Used: Mutagenesis, Variant Assay

    eml4 alk  (ATCC)


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    ATCC eml4 alk
    Characteristics of the lung cell lines included in the study. AD, adenocarcinoma; ATCC, American Type Culture Collection; NE, normal epithelial; UCSF, University California San Francisco; UTSW, University of Texas Southwestern.
    Eml4 Alk, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eml4 alk/product/ATCC
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    1) Product Images from "Digital multiplexed analysis of circular RNAs in FFPE and fresh non‐small cell lung cancer specimens"

    Article Title: Digital multiplexed analysis of circular RNAs in FFPE and fresh non‐small cell lung cancer specimens

    Journal: Molecular Oncology

    doi: 10.1002/1878-0261.13182

    Characteristics of the lung cell lines included in the study. AD, adenocarcinoma; ATCC, American Type Culture Collection; NE, normal epithelial; UCSF, University California San Francisco; UTSW, University of Texas Southwestern.
    Figure Legend Snippet: Characteristics of the lung cell lines included in the study. AD, adenocarcinoma; ATCC, American Type Culture Collection; NE, normal epithelial; UCSF, University California San Francisco; UTSW, University of Texas Southwestern.

    Techniques Used: Mutagenesis, Variant Assay

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    ATCC ccl 185ig a549 eml4 alk cells
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    ATCC ccl 185ig cell line
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    ATCC adenocarcinomic human alveolar basal epithelial cell line
    Determination of influenza A virus replication in cells with knocked-down 14-3-3γ expression. <t>A549</t> cells were transfected with 14-3-3γ siRNA for 24 h and then infected with either influenza A wild-type virus PR8 ( A ) or PR8-NS1/1-98 mutant virus ( B ) at an MOI of 0.001. At the indicated time points, the supernatants of the infected cells were collected for titration of the infectious viral particles with a plaque formation assay. The cells were extracted to analyze the protein expression by immunoblotting with anti-14-3-3γ, anti-influenza A virus NP, and anti-β-tubulin antibodies. The experiments were performed in triplicate and the results were analyzed with Student’s t -test. *, p < 0.05.
    Adenocarcinomic Human Alveolar Basal Epithelial Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC eml4 alk
    Characteristics of the lung cell lines included in the study. AD, adenocarcinoma; ATCC, American Type Culture Collection; NE, normal epithelial; UCSF, University California San Francisco; UTSW, University of Texas Southwestern.
    Eml4 Alk, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Determination of influenza A virus replication in cells with knocked-down 14-3-3γ expression. A549 cells were transfected with 14-3-3γ siRNA for 24 h and then infected with either influenza A wild-type virus PR8 ( A ) or PR8-NS1/1-98 mutant virus ( B ) at an MOI of 0.001. At the indicated time points, the supernatants of the infected cells were collected for titration of the infectious viral particles with a plaque formation assay. The cells were extracted to analyze the protein expression by immunoblotting with anti-14-3-3γ, anti-influenza A virus NP, and anti-β-tubulin antibodies. The experiments were performed in triplicate and the results were analyzed with Student’s t -test. *, p < 0.05.

    Journal: Pathogens

    Article Title: Interactome Profiling of N-Terminus-Truncated NS1 Protein of Influenza A Virus Reveals Role of 14-3-3γ in Virus Replication

    doi: 10.3390/pathogens11070733

    Figure Lengend Snippet: Determination of influenza A virus replication in cells with knocked-down 14-3-3γ expression. A549 cells were transfected with 14-3-3γ siRNA for 24 h and then infected with either influenza A wild-type virus PR8 ( A ) or PR8-NS1/1-98 mutant virus ( B ) at an MOI of 0.001. At the indicated time points, the supernatants of the infected cells were collected for titration of the infectious viral particles with a plaque formation assay. The cells were extracted to analyze the protein expression by immunoblotting with anti-14-3-3γ, anti-influenza A virus NP, and anti-β-tubulin antibodies. The experiments were performed in triplicate and the results were analyzed with Student’s t -test. *, p < 0.05.

    Article Snippet: Human Embryonic Kidney 293T cell line (293T), adenocarcinomic human alveolar basal epithelial cell line (A549), and Madin Darby Canine Kidney (MDCK) cell line (obtained from the American Type Culture Collection (ATCC), Rockville, MD, USA) were cultivated in DMEM (Gibco, Waltham, MA, USA) supplemented with 10% fetal bovine serum (Gibco), 1% non-essential amino acids (Gibco), 1% penicillin-streptomycin (Gibco), and 1% L-glutamine (Gibco).

    Techniques: Expressing, Transfection, Infection, Mutagenesis, Titration, Plaque Formation Assay, Western Blot

    Examination of IFN-β mRNA expression in 14-3-3γ knockdown cells infected with influenza A virus. A549 cells were transfected with 14-3-3γ siRNA for 24 h and then infected with influenza A PR8 wild-type virus at an MOI of 2. At 3, 6, 9, and 12 h post-infection (PI), the total RNA of the infected cells was extracted to determine IFN-β mRNA expression with RT-qPCR. Cell extracts were analyzed for 14-3-3γ, phosphorylated IRF3 (pIRF3), total IRF3, influenza virus NP, and β-tubulin with immunoblotting.

    Journal: Pathogens

    Article Title: Interactome Profiling of N-Terminus-Truncated NS1 Protein of Influenza A Virus Reveals Role of 14-3-3γ in Virus Replication

    doi: 10.3390/pathogens11070733

    Figure Lengend Snippet: Examination of IFN-β mRNA expression in 14-3-3γ knockdown cells infected with influenza A virus. A549 cells were transfected with 14-3-3γ siRNA for 24 h and then infected with influenza A PR8 wild-type virus at an MOI of 2. At 3, 6, 9, and 12 h post-infection (PI), the total RNA of the infected cells was extracted to determine IFN-β mRNA expression with RT-qPCR. Cell extracts were analyzed for 14-3-3γ, phosphorylated IRF3 (pIRF3), total IRF3, influenza virus NP, and β-tubulin with immunoblotting.

    Article Snippet: Human Embryonic Kidney 293T cell line (293T), adenocarcinomic human alveolar basal epithelial cell line (A549), and Madin Darby Canine Kidney (MDCK) cell line (obtained from the American Type Culture Collection (ATCC), Rockville, MD, USA) were cultivated in DMEM (Gibco, Waltham, MA, USA) supplemented with 10% fetal bovine serum (Gibco), 1% non-essential amino acids (Gibco), 1% penicillin-streptomycin (Gibco), and 1% L-glutamine (Gibco).

    Techniques: Expressing, Infection, Transfection, Quantitative RT-PCR, Western Blot

    Analysis of influenza A viral RNAs in 14-3-3γ knockdown cells. 14-3-3γ expression of A549 cells was knocked down and then the cells were infected with influenza A PR8 wild-type virus for 36 h. Total RNA of the infected cells was extracted to examine the levels of vRNA, cRNA, and viral mRNA, respectively, using RT-qPCR (right panel). The protein expression of 14-3-3γ, influenza A NP, and β-tubulin was assayed with immunoblotting (left panel). The experiments were performed in triplicate and the results were analyzed with Student’s t -test. *, p < 0.05; **, p < 0.01; ***, p < 0.001.

    Journal: Pathogens

    Article Title: Interactome Profiling of N-Terminus-Truncated NS1 Protein of Influenza A Virus Reveals Role of 14-3-3γ in Virus Replication

    doi: 10.3390/pathogens11070733

    Figure Lengend Snippet: Analysis of influenza A viral RNAs in 14-3-3γ knockdown cells. 14-3-3γ expression of A549 cells was knocked down and then the cells were infected with influenza A PR8 wild-type virus for 36 h. Total RNA of the infected cells was extracted to examine the levels of vRNA, cRNA, and viral mRNA, respectively, using RT-qPCR (right panel). The protein expression of 14-3-3γ, influenza A NP, and β-tubulin was assayed with immunoblotting (left panel). The experiments were performed in triplicate and the results were analyzed with Student’s t -test. *, p < 0.05; **, p < 0.01; ***, p < 0.001.

    Article Snippet: Human Embryonic Kidney 293T cell line (293T), adenocarcinomic human alveolar basal epithelial cell line (A549), and Madin Darby Canine Kidney (MDCK) cell line (obtained from the American Type Culture Collection (ATCC), Rockville, MD, USA) were cultivated in DMEM (Gibco, Waltham, MA, USA) supplemented with 10% fetal bovine serum (Gibco), 1% non-essential amino acids (Gibco), 1% penicillin-streptomycin (Gibco), and 1% L-glutamine (Gibco).

    Techniques: Expressing, Infection, Quantitative RT-PCR, Western Blot

    Characteristics of the lung cell lines included in the study. AD, adenocarcinoma; ATCC, American Type Culture Collection; NE, normal epithelial; UCSF, University California San Francisco; UTSW, University of Texas Southwestern.

    Journal: Molecular Oncology

    Article Title: Digital multiplexed analysis of circular RNAs in FFPE and fresh non‐small cell lung cancer specimens

    doi: 10.1002/1878-0261.13182

    Figure Lengend Snippet: Characteristics of the lung cell lines included in the study. AD, adenocarcinoma; ATCC, American Type Culture Collection; NE, normal epithelial; UCSF, University California San Francisco; UTSW, University of Texas Southwestern.

    Article Snippet: NCI‐H2228 , , ALK , EML4‐ALK , variant 1 , ATCC.

    Techniques: Mutagenesis, Variant Assay