mrc 5 ccl 171 human embryo fibroblasts  (ATCC)


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    ATCC mrc 5 ccl 171 human embryo fibroblasts
    Mrc 5 Ccl 171 Human Embryo Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mrc 5 ccl 171 human embryo fibroblasts  (ATCC)


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    ATCC mrc 5 ccl 171 human embryo fibroblasts
    VR1814 proliferation in <t>MRC-5,</t> ARPE-19 and HMEC-1. 1000 IU/well of VR1814 were seeded in MRC-5, ARPE-19, and HMEC-1 in 96-well plates. Culture medium and monolayers were collected at 0, 1, 3, 5, 8, 10, 13, 16, 18, and 22 h.p.i. and analyzed by RT-qPCR with primers targeting the hCMV IE1 gene transcript. Each point represents the mean with the standard deviation of the data set (n = 4).
    Mrc 5 Ccl 171 Human Embryo Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Evaluation of a Reverse Transcription-Quantitative Polymerase Chain Reaction (RT-qPCR)-Based Microneutralization Assay for Assessing Clinical Human Cytomegalovirus-Neutralizing Antibody Activity"

    Article Title: Evaluation of a Reverse Transcription-Quantitative Polymerase Chain Reaction (RT-qPCR)-Based Microneutralization Assay for Assessing Clinical Human Cytomegalovirus-Neutralizing Antibody Activity

    Journal: Microorganisms

    doi: 10.3390/microorganisms12040742

    VR1814 proliferation in MRC-5, ARPE-19 and HMEC-1. 1000 IU/well of VR1814 were seeded in MRC-5, ARPE-19, and HMEC-1 in 96-well plates. Culture medium and monolayers were collected at 0, 1, 3, 5, 8, 10, 13, 16, 18, and 22 h.p.i. and analyzed by RT-qPCR with primers targeting the hCMV IE1 gene transcript. Each point represents the mean with the standard deviation of the data set (n = 4).
    Figure Legend Snippet: VR1814 proliferation in MRC-5, ARPE-19 and HMEC-1. 1000 IU/well of VR1814 were seeded in MRC-5, ARPE-19, and HMEC-1 in 96-well plates. Culture medium and monolayers were collected at 0, 1, 3, 5, 8, 10, 13, 16, 18, and 22 h.p.i. and analyzed by RT-qPCR with primers targeting the hCMV IE1 gene transcript. Each point represents the mean with the standard deviation of the data set (n = 4).

    Techniques Used: Quantitative RT-PCR, Standard Deviation

    RNA yields of hCMV from clinical samples at 20 vs. 48 h.p.i. by RT-qPCR. 200 μL of hCMV positive samples (3 breast milk, 1 saliva and 2 urine) were used to inoculate MRC-5, ARPE-19, and HMEC-1 in 96-well plates. Culture medium and monolayers were harvested at 20 and 48 h.p.i. and analyzed by RT-qPCR.
    Figure Legend Snippet: RNA yields of hCMV from clinical samples at 20 vs. 48 h.p.i. by RT-qPCR. 200 μL of hCMV positive samples (3 breast milk, 1 saliva and 2 urine) were used to inoculate MRC-5, ARPE-19, and HMEC-1 in 96-well plates. Culture medium and monolayers were harvested at 20 and 48 h.p.i. and analyzed by RT-qPCR.

    Techniques Used: Quantitative RT-PCR

    Correlation between the RT-qPCR and immunostaining assays in determining infectious hCMV virions. A series of diluted VR1814 was used to inoculate MRC-5 cells in two 96-well plates. The cells in each plate were analyzed using either RT-qPCR or immunostaining. The results were plotted on a scatter plot, with data from the immunostaining assay on the y -axis and data from the RT-qPCR assay on the x -axis. A regression line was drawn based on the data (n = 6).
    Figure Legend Snippet: Correlation between the RT-qPCR and immunostaining assays in determining infectious hCMV virions. A series of diluted VR1814 was used to inoculate MRC-5 cells in two 96-well plates. The cells in each plate were analyzed using either RT-qPCR or immunostaining. The results were plotted on a scatter plot, with data from the immunostaining assay on the y -axis and data from the RT-qPCR assay on the x -axis. A regression line was drawn based on the data (n = 6).

    Techniques Used: Quantitative RT-PCR, Immunostaining

    Neutralization efficacy curves of VR1814 generated by (A) RT-qPCR-based and (B) immunostaining-based neutralization assays. A 2-fold serial dilution of IgG Hizentra from 50 mg/mL to 0.38 μg/mL was incubated with an equal volume of 1000 IU of VR1814 for 1 h. The mixture was used to inoculate MRC-5, ARPE-19 and HMEC-1 in 96-well plates and analyzed by ( A ) RT-qPCR and ( B ) immunostaining respectively (n = 3).
    Figure Legend Snippet: Neutralization efficacy curves of VR1814 generated by (A) RT-qPCR-based and (B) immunostaining-based neutralization assays. A 2-fold serial dilution of IgG Hizentra from 50 mg/mL to 0.38 μg/mL was incubated with an equal volume of 1000 IU of VR1814 for 1 h. The mixture was used to inoculate MRC-5, ARPE-19 and HMEC-1 in 96-well plates and analyzed by ( A ) RT-qPCR and ( B ) immunostaining respectively (n = 3).

    Techniques Used: Neutralization, Generated, Quantitative RT-PCR, Immunostaining, Serial Dilution, Incubation

    Percentages of antibody neutralization efficacy of hCMV from a representative urine sample and VR1814 detected by ( A ) immunostaining-based and ( B ) RT-qPCR-based neutralization assays. IgG Cytogam at 1280 (high) and 640 μg/mL (low), anti-gB #1 at 50 and 10 μg/mL, anti-gB #2 at 50 and 7.5 μg/mL, anti-gH at 50 and 35 μg/mL, and anti-pentamer at 50 and 25 μg/mL were incubated with equal volume of urine for 1 h and then used to inoculate MRC-5 in 96-well plates. The 96-well plates were assessed by ( A ) RT-qPCR and ( B ) immunostaining respectively after incubation (n = 3).
    Figure Legend Snippet: Percentages of antibody neutralization efficacy of hCMV from a representative urine sample and VR1814 detected by ( A ) immunostaining-based and ( B ) RT-qPCR-based neutralization assays. IgG Cytogam at 1280 (high) and 640 μg/mL (low), anti-gB #1 at 50 and 10 μg/mL, anti-gB #2 at 50 and 7.5 μg/mL, anti-gH at 50 and 35 μg/mL, and anti-pentamer at 50 and 25 μg/mL were incubated with equal volume of urine for 1 h and then used to inoculate MRC-5 in 96-well plates. The 96-well plates were assessed by ( A ) RT-qPCR and ( B ) immunostaining respectively after incubation (n = 3).

    Techniques Used: Neutralization, Immunostaining, Quantitative RT-PCR, Incubation

    mrc 5 ccl 171  (ATCC)


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    ATCC mrc 5 ccl 171
    Mrc 5 Ccl 171, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mrc 5 ccl 171  (ATCC)


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    ATCC mrc 5 ccl 171
    Mrc 5 Ccl 171, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ccl 171  (ATCC)


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    ATCC ccl 171
    Ccl 171, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mrc 5 atcc ccl 171 lung fibroblasts  (ATCC)


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    ATCC mrc 5 atcc ccl 171 lung fibroblasts
    Mrc 5 Atcc Ccl 171 Lung Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mrc5 ccl 171 cell lines  (ATCC)


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    ATCC mrc5 ccl 171 cell lines
    RSV-infection-induced expression of A3 genes in human lung cells. ( A ) RSV infection induces A3 family gene expression in normal human lung fibroblasts <t>MRC5</t> cells infected with RSV at MOI of 1 for 72 h determined by qPCR. Expression changes are presented as fold-change relative to mock (PBS) non-infected cells. * p < 0.05 compared with non-infected cells. ( B ) Time course of induced expression of A3 genes in A549 cells infected with RSV at MOI of 1. * p < 0.05. ( C ) Expression of A3 genes in A549 cells stably expressing scrambled shRNA or p53 shRNAi vectors (p53sh-3755 or p53sh-3756) and infected for 72 h with RSV at MOI of 1. Values are displayed as fold-changes relative to their respective parental mock non-infected A549 cells. * p < 0.05 compared to scramble cells. ( D ) Time course of induced expression of A3 genes in H1299 cells infected with RSV at MOI of 1. * p < 0.05 compared with non-infected cells.
    Mrc5 Ccl 171 Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "APOBEC3G Is a p53-Dependent Restriction Factor in Respiratory Syncytial Virus Infection of Human Cells Included in the p53/Immune Axis"

    Article Title: APOBEC3G Is a p53-Dependent Restriction Factor in Respiratory Syncytial Virus Infection of Human Cells Included in the p53/Immune Axis

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms242316793

    RSV-infection-induced expression of A3 genes in human lung cells. ( A ) RSV infection induces A3 family gene expression in normal human lung fibroblasts MRC5 cells infected with RSV at MOI of 1 for 72 h determined by qPCR. Expression changes are presented as fold-change relative to mock (PBS) non-infected cells. * p < 0.05 compared with non-infected cells. ( B ) Time course of induced expression of A3 genes in A549 cells infected with RSV at MOI of 1. * p < 0.05. ( C ) Expression of A3 genes in A549 cells stably expressing scrambled shRNA or p53 shRNAi vectors (p53sh-3755 or p53sh-3756) and infected for 72 h with RSV at MOI of 1. Values are displayed as fold-changes relative to their respective parental mock non-infected A549 cells. * p < 0.05 compared to scramble cells. ( D ) Time course of induced expression of A3 genes in H1299 cells infected with RSV at MOI of 1. * p < 0.05 compared with non-infected cells.
    Figure Legend Snippet: RSV-infection-induced expression of A3 genes in human lung cells. ( A ) RSV infection induces A3 family gene expression in normal human lung fibroblasts MRC5 cells infected with RSV at MOI of 1 for 72 h determined by qPCR. Expression changes are presented as fold-change relative to mock (PBS) non-infected cells. * p < 0.05 compared with non-infected cells. ( B ) Time course of induced expression of A3 genes in A549 cells infected with RSV at MOI of 1. * p < 0.05. ( C ) Expression of A3 genes in A549 cells stably expressing scrambled shRNA or p53 shRNAi vectors (p53sh-3755 or p53sh-3756) and infected for 72 h with RSV at MOI of 1. Values are displayed as fold-changes relative to their respective parental mock non-infected A549 cells. * p < 0.05 compared to scramble cells. ( D ) Time course of induced expression of A3 genes in H1299 cells infected with RSV at MOI of 1. * p < 0.05 compared with non-infected cells.

    Techniques Used: Infection, Expressing, Stable Transfection, shRNA

    human broblast mrc 5 ccl 171  (ATCC)


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    ATCC human broblast mrc 5 ccl 171
    Human Broblast Mrc 5 Ccl 171, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ccl 171  (ATCC)


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    ATCC ccl 171
    Ccl 171, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ccl 171  (ATCC)


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    ATCC ccl 171
    Ccl 171, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC mrc 5 ccl 171 human embryo fibroblasts
    Mrc 5 Ccl 171 Human Embryo Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC mrc 5 ccl 171
    Mrc 5 Ccl 171, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC ccl 171
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    ATCC mrc 5 atcc ccl 171 lung fibroblasts
    Mrc 5 Atcc Ccl 171 Lung Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC mrc5 ccl 171 cell lines
    RSV-infection-induced expression of A3 genes in human lung cells. ( A ) RSV infection induces A3 family gene expression in normal human lung fibroblasts <t>MRC5</t> cells infected with RSV at MOI of 1 for 72 h determined by qPCR. Expression changes are presented as fold-change relative to mock (PBS) non-infected cells. * p < 0.05 compared with non-infected cells. ( B ) Time course of induced expression of A3 genes in A549 cells infected with RSV at MOI of 1. * p < 0.05. ( C ) Expression of A3 genes in A549 cells stably expressing scrambled shRNA or p53 shRNAi vectors (p53sh-3755 or p53sh-3756) and infected for 72 h with RSV at MOI of 1. Values are displayed as fold-changes relative to their respective parental mock non-infected A549 cells. * p < 0.05 compared to scramble cells. ( D ) Time course of induced expression of A3 genes in H1299 cells infected with RSV at MOI of 1. * p < 0.05 compared with non-infected cells.
    Mrc5 Ccl 171 Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC human broblast mrc 5 ccl 171
    RSV-infection-induced expression of A3 genes in human lung cells. ( A ) RSV infection induces A3 family gene expression in normal human lung fibroblasts <t>MRC5</t> cells infected with RSV at MOI of 1 for 72 h determined by qPCR. Expression changes are presented as fold-change relative to mock (PBS) non-infected cells. * p < 0.05 compared with non-infected cells. ( B ) Time course of induced expression of A3 genes in A549 cells infected with RSV at MOI of 1. * p < 0.05. ( C ) Expression of A3 genes in A549 cells stably expressing scrambled shRNA or p53 shRNAi vectors (p53sh-3755 or p53sh-3756) and infected for 72 h with RSV at MOI of 1. Values are displayed as fold-changes relative to their respective parental mock non-infected A549 cells. * p < 0.05 compared to scramble cells. ( D ) Time course of induced expression of A3 genes in H1299 cells infected with RSV at MOI of 1. * p < 0.05 compared with non-infected cells.
    Human Broblast Mrc 5 Ccl 171, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    RSV-infection-induced expression of A3 genes in human lung cells. ( A ) RSV infection induces A3 family gene expression in normal human lung fibroblasts MRC5 cells infected with RSV at MOI of 1 for 72 h determined by qPCR. Expression changes are presented as fold-change relative to mock (PBS) non-infected cells. * p < 0.05 compared with non-infected cells. ( B ) Time course of induced expression of A3 genes in A549 cells infected with RSV at MOI of 1. * p < 0.05. ( C ) Expression of A3 genes in A549 cells stably expressing scrambled shRNA or p53 shRNAi vectors (p53sh-3755 or p53sh-3756) and infected for 72 h with RSV at MOI of 1. Values are displayed as fold-changes relative to their respective parental mock non-infected A549 cells. * p < 0.05 compared to scramble cells. ( D ) Time course of induced expression of A3 genes in H1299 cells infected with RSV at MOI of 1. * p < 0.05 compared with non-infected cells.

    Journal: International Journal of Molecular Sciences

    Article Title: APOBEC3G Is a p53-Dependent Restriction Factor in Respiratory Syncytial Virus Infection of Human Cells Included in the p53/Immune Axis

    doi: 10.3390/ijms242316793

    Figure Lengend Snippet: RSV-infection-induced expression of A3 genes in human lung cells. ( A ) RSV infection induces A3 family gene expression in normal human lung fibroblasts MRC5 cells infected with RSV at MOI of 1 for 72 h determined by qPCR. Expression changes are presented as fold-change relative to mock (PBS) non-infected cells. * p < 0.05 compared with non-infected cells. ( B ) Time course of induced expression of A3 genes in A549 cells infected with RSV at MOI of 1. * p < 0.05. ( C ) Expression of A3 genes in A549 cells stably expressing scrambled shRNA or p53 shRNAi vectors (p53sh-3755 or p53sh-3756) and infected for 72 h with RSV at MOI of 1. Values are displayed as fold-changes relative to their respective parental mock non-infected A549 cells. * p < 0.05 compared to scramble cells. ( D ) Time course of induced expression of A3 genes in H1299 cells infected with RSV at MOI of 1. * p < 0.05 compared with non-infected cells.

    Article Snippet: ATCC A549 (CCL-185), H1299 (CRL-5803), HEp2 (CCL-23), and MRC5 (CCL-171) cell lines were cultured as described by the vendor (ATCC).

    Techniques: Infection, Expressing, Stable Transfection, shRNA