shuffle  (New England Biolabs)


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  • 96
    Name:
    SHuffle Express Competent E coli
    Description:
    SHuffle Express Competent E coli 12x0 05 ml
    Catalog Number:
    c3028j
    Price:
    228
    Size:
    12x0 05 ml
    Category:
    Competent Bacteria
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    Structured Review

    New England Biolabs shuffle
    SHuffle Express Competent E coli
    SHuffle Express Competent E coli 12x0 05 ml
    https://www.bioz.com/result/shuffle/product/New England Biolabs
    Average 96 stars, based on 54 article reviews
    Price from $9.99 to $1999.99
    shuffle - by Bioz Stars, 2020-07
    96/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Soluble expression of recombinant midgut zymogen (native propeptide) proteases from the Aedes aegypti Mosquito Utilizing E. coli as a host
    Article Snippet: .. Expression of recombinant proteases using SHuffle® (NEB) E. coli cells and Bis-Tris gel analysis The most commonly used bacterial strain for expression of genes cloned into the pET vector system are the BL21(DE3) and E. coli K12 lineage strains [ ]. ..

    Positron Emission Tomography:

    Article Title: Soluble expression of recombinant midgut zymogen (native propeptide) proteases from the Aedes aegypti Mosquito Utilizing E. coli as a host
    Article Snippet: .. Expression of recombinant proteases using SHuffle® (NEB) E. coli cells and Bis-Tris gel analysis The most commonly used bacterial strain for expression of genes cloned into the pET vector system are the BL21(DE3) and E. coli K12 lineage strains [ ]. ..

    Construct:

    Article Title: Llama immunization with full-length VAR2CSA generates cross-reactive and inhibitory single-domain antibodies against the DBL1X domain
    Article Snippet: .. The single-domain constructs 3D7-DBL1X, 3D7-DBL2X, FCR3-DBL3X, 3D7-DBL5ε, 3D7-DBL6ε, FCR3-DBL6ε and the multi-domain FCR3-DBL3X-4ε were expressed either in the Rosetta-Gami (Novagen), Origami B (Novagen) or SHuffle® (NEB Biolabs) strains of E. coli as cytoplasmic soluble proteins . .. Proteins were purified using a metal affinity column (TALON, Clontech), followed by a heparin affinity column (GE Healthcare) when needed, and by a gel filtration chromatography (Superdex 75 16/60, GE Healthcare), as previously described .

    Purification:

    Article Title: Llama immunization with full-length VAR2CSA generates cross-reactive and inhibitory single-domain antibodies against the DBL1X domain
    Article Snippet: .. Expression and purification of nanobodies Nanobodies were expressed in the periplasm of either BL21 (Novagen) or SHuffle® (NEB Biolabs) strains of E. coli . .. Expression in the periplasmic space of BL21 was done using TB (Terrific Broth) medium and fermentation left for 20h at 28°C after 0.5 mM IPTG (isopropyl thiogalactoside) induction.

    Article Title: Nanobodies: site-specific labeling for super-resolution imaging, rapid epitope-mapping and native protein complex isolation
    Article Snippet: .. Expression and purification of nanobodies Nanobodies with protease-cleavable affinity tags or engineered cysteines were routinely expressed in the cytoplasm of E. coli BLR (BL21 derivative; Novagen) or E. coli SHuffle Express (New England Biolabs). ..

    Expressing:

    Article Title: Structures of Xenopus Embryonic Epidermal Lectin Reveal a Conserved Mechanism of Microbial Glycan Recognition *
    Article Snippet: .. The expression plasmid was transformed into Escherichia coli SHuffle® Express (New England Biolabs). ..

    Article Title: Llama immunization with full-length VAR2CSA generates cross-reactive and inhibitory single-domain antibodies against the DBL1X domain
    Article Snippet: .. Expression and purification of nanobodies Nanobodies were expressed in the periplasm of either BL21 (Novagen) or SHuffle® (NEB Biolabs) strains of E. coli . .. Expression in the periplasmic space of BL21 was done using TB (Terrific Broth) medium and fermentation left for 20h at 28°C after 0.5 mM IPTG (isopropyl thiogalactoside) induction.

    Article Title: Soluble expression of recombinant midgut zymogen (native propeptide) proteases from the Aedes aegypti Mosquito Utilizing E. coli as a host
    Article Snippet: .. Expression of recombinant proteases using SHuffle® (NEB) E. coli cells and Bis-Tris gel analysis The most commonly used bacterial strain for expression of genes cloned into the pET vector system are the BL21(DE3) and E. coli K12 lineage strains [ ]. ..

    Article Title: Nanobodies: site-specific labeling for super-resolution imaging, rapid epitope-mapping and native protein complex isolation
    Article Snippet: .. Expression and purification of nanobodies Nanobodies with protease-cleavable affinity tags or engineered cysteines were routinely expressed in the cytoplasm of E. coli BLR (BL21 derivative; Novagen) or E. coli SHuffle Express (New England Biolabs). ..

    Lysis:

    Article Title: Chitin-based barrier immunity and its loss predated mucus-colonization by indigenous gut microbiota
    Article Snippet: .. Recombinant proteins were expressed in SHuffle Express competent Escherichia coli (New England BioLabs, USA), followed by cell lysis with B-Per reagent (Thermo Fisher Scientific, USA) and affinity purification with Talon resin (Clontech, USA). .. Purification was assessed by SDS-PAGE and western blotting using anti-His-tag mAb-HRP-DirecT antibody (Code number: D291-7, Medical & Biological Laboratories, Japan) at 1:4000 (v/v) dilution with ImmunoStar zeta chemiluminescence reagent (Wako, Japan) (Supplementary Fig. ).

    Transformation Assay:

    Article Title: Structures of Xenopus Embryonic Epidermal Lectin Reveal a Conserved Mechanism of Microbial Glycan Recognition *
    Article Snippet: .. The expression plasmid was transformed into Escherichia coli SHuffle® Express (New England Biolabs). ..

    Article Title: Structure and functional analysis of the Legionella pneumophila chitinase ChiA reveals a novel mechanism of metal-dependent mucin degradation.
    Article Snippet: .. These were transformed into E. coli SHuffle cells (New England Biolabs) and grown at 37˚C in LB media with 100 μg/ml ampicillin. .. Expression was induced with 1 mM isopropyl-d-1-thiogalactopyranoside (IPTG) at an OD600nm of 0.6 and cells were harvested after growth overnight at 18˚C.

    Recombinant:

    Article Title: Soluble expression of recombinant midgut zymogen (native propeptide) proteases from the Aedes aegypti Mosquito Utilizing E. coli as a host
    Article Snippet: .. Expression of recombinant proteases using SHuffle® (NEB) E. coli cells and Bis-Tris gel analysis The most commonly used bacterial strain for expression of genes cloned into the pET vector system are the BL21(DE3) and E. coli K12 lineage strains [ ]. ..

    Article Title: Chitin-based barrier immunity and its loss predated mucus-colonization by indigenous gut microbiota
    Article Snippet: .. Recombinant proteins were expressed in SHuffle Express competent Escherichia coli (New England BioLabs, USA), followed by cell lysis with B-Per reagent (Thermo Fisher Scientific, USA) and affinity purification with Talon resin (Clontech, USA). .. Purification was assessed by SDS-PAGE and western blotting using anti-His-tag mAb-HRP-DirecT antibody (Code number: D291-7, Medical & Biological Laboratories, Japan) at 1:4000 (v/v) dilution with ImmunoStar zeta chemiluminescence reagent (Wako, Japan) (Supplementary Fig. ).

    Affinity Purification:

    Article Title: Chitin-based barrier immunity and its loss predated mucus-colonization by indigenous gut microbiota
    Article Snippet: .. Recombinant proteins were expressed in SHuffle Express competent Escherichia coli (New England BioLabs, USA), followed by cell lysis with B-Per reagent (Thermo Fisher Scientific, USA) and affinity purification with Talon resin (Clontech, USA). .. Purification was assessed by SDS-PAGE and western blotting using anti-His-tag mAb-HRP-DirecT antibody (Code number: D291-7, Medical & Biological Laboratories, Japan) at 1:4000 (v/v) dilution with ImmunoStar zeta chemiluminescence reagent (Wako, Japan) (Supplementary Fig. ).

    Plasmid Preparation:

    Article Title: Structures of Xenopus Embryonic Epidermal Lectin Reveal a Conserved Mechanism of Microbial Glycan Recognition *
    Article Snippet: .. The expression plasmid was transformed into Escherichia coli SHuffle® Express (New England Biolabs). ..

    Article Title: Soluble expression of recombinant midgut zymogen (native propeptide) proteases from the Aedes aegypti Mosquito Utilizing E. coli as a host
    Article Snippet: .. Expression of recombinant proteases using SHuffle® (NEB) E. coli cells and Bis-Tris gel analysis The most commonly used bacterial strain for expression of genes cloned into the pET vector system are the BL21(DE3) and E. coli K12 lineage strains [ ]. ..

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  • 96
    New England Biolabs shuffle
    Shuffle, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/shuffle/product/New England Biolabs
    Average 96 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    shuffle - by Bioz Stars, 2020-07
    96/100 stars
      Buy from Supplier

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