e coli cloning strain c3019  (New England Biolabs)


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    Structured Review

    New England Biolabs e coli cloning strain c3019
    E Coli Cloning Strain C3019, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli cloning strain c3019/product/New England Biolabs
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    e coli cloning strain c3019 - by Bioz Stars, 2019-10
    80/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Characterization of Transcription Factors That Regulate the Type IV Secretion System and Riboflavin Biosynthesis in Wolbachia of Brugia malayi
    Article Snippet: The ribAp-lacZ fusion was amplified with primers acggagacaUCGCTCTGCCGGTGGTTACCA and agggaaagUAACCTATAAAAATAGGCGTATCACGAGG derived from the ribAp-lacZ -pNK1415 construct. .. 10 μl of the ribAp-lacZ insert and 1 μl of the pACYC184 backbone were then incubated with 1 μl of USER enzyme at room temperature for 15 min before being transformed into E. coli cloning strain C3019 (NEB) to generate the ribAp-lacZ -pACYC184 reporter plasmid. .. Both ribAp-lacZ -pACYC184 and wBmxR1- pET21a or wBmxR2 -pET21a were transformed into E. coli strain C2566 (NEB) for measuring β-galactosidase activity measurement.

    Negative Control:

    Article Title: Characterization of Transcription Factors That Regulate the Type IV Secretion System and Riboflavin Biosynthesis in Wolbachia of Brugia malayi
    Article Snippet: 10 μl of the ribAp-lacZ insert and 1 μl of the pACYC184 backbone were then incubated with 1 μl of USER enzyme at room temperature for 15 min before being transformed into E. coli cloning strain C3019 (NEB) to generate the ribAp-lacZ -pACYC184 reporter plasmid. .. Both ribAp-lacZ -pACYC184 and wBmxR1- pET21a or wBmxR2 -pET21a were transformed into E. coli strain C2566 (NEB) for measuring β-galactosidase activity measurement.

    Amplification:

    Article Title: Characterization of Transcription Factors That Regulate the Type IV Secretion System and Riboflavin Biosynthesis in Wolbachia of Brugia malayi
    Article Snippet: The ribAp-lacZ fusion was amplified with primers acggagacaUCGCTCTGCCGGTGGTTACCA and agggaaagUAACCTATAAAAATAGGCGTATCACGAGG derived from the ribAp-lacZ -pNK1415 construct. .. 10 μl of the ribAp-lacZ insert and 1 μl of the pACYC184 backbone were then incubated with 1 μl of USER enzyme at room temperature for 15 min before being transformed into E. coli cloning strain C3019 (NEB) to generate the ribAp-lacZ -pACYC184 reporter plasmid.

    Low Copy Number:

    Article Title: Characterization of Transcription Factors That Regulate the Type IV Secretion System and Riboflavin Biosynthesis in Wolbachia of Brugia malayi
    Article Snippet: The USER cloning method (NEB) was then utilized to clone the ribAp-lacZ fusion into low copy number plasmid pACYC184 (NEB). .. 10 μl of the ribAp-lacZ insert and 1 μl of the pACYC184 backbone were then incubated with 1 μl of USER enzyme at room temperature for 15 min before being transformed into E. coli cloning strain C3019 (NEB) to generate the ribAp-lacZ -pACYC184 reporter plasmid.

    Construct:

    Article Title: Characterization of Transcription Factors That Regulate the Type IV Secretion System and Riboflavin Biosynthesis in Wolbachia of Brugia malayi
    Article Snippet: The ribAp-lacZ fusion was amplified with primers acggagacaUCGCTCTGCCGGTGGTTACCA and agggaaagUAACCTATAAAAATAGGCGTATCACGAGG derived from the ribAp-lacZ -pNK1415 construct. .. 10 μl of the ribAp-lacZ insert and 1 μl of the pACYC184 backbone were then incubated with 1 μl of USER enzyme at room temperature for 15 min before being transformed into E. coli cloning strain C3019 (NEB) to generate the ribAp-lacZ -pACYC184 reporter plasmid.

    Incubation:

    Article Title: Characterization of Transcription Factors That Regulate the Type IV Secretion System and Riboflavin Biosynthesis in Wolbachia of Brugia malayi
    Article Snippet: The ribAp-lacZ fusion was amplified with primers acggagacaUCGCTCTGCCGGTGGTTACCA and agggaaagUAACCTATAAAAATAGGCGTATCACGAGG derived from the ribAp-lacZ -pNK1415 construct. .. 10 μl of the ribAp-lacZ insert and 1 μl of the pACYC184 backbone were then incubated with 1 μl of USER enzyme at room temperature for 15 min before being transformed into E. coli cloning strain C3019 (NEB) to generate the ribAp-lacZ -pACYC184 reporter plasmid. .. Both ribAp-lacZ -pACYC184 and wBmxR1- pET21a or wBmxR2 -pET21a were transformed into E. coli strain C2566 (NEB) for measuring β-galactosidase activity measurement.

    Activity Assay:

    Article Title: Characterization of Transcription Factors That Regulate the Type IV Secretion System and Riboflavin Biosynthesis in Wolbachia of Brugia malayi
    Article Snippet: 10 μl of the ribAp-lacZ insert and 1 μl of the pACYC184 backbone were then incubated with 1 μl of USER enzyme at room temperature for 15 min before being transformed into E. coli cloning strain C3019 (NEB) to generate the ribAp-lacZ -pACYC184 reporter plasmid. .. The pET21a vector alone was used as a negative control.

    Derivative Assay:

    Article Title: Characterization of Transcription Factors That Regulate the Type IV Secretion System and Riboflavin Biosynthesis in Wolbachia of Brugia malayi
    Article Snippet: The ribAp-lacZ fusion was amplified with primers acggagacaUCGCTCTGCCGGTGGTTACCA and agggaaagUAACCTATAAAAATAGGCGTATCACGAGG derived from the ribAp-lacZ -pNK1415 construct. .. 10 μl of the ribAp-lacZ insert and 1 μl of the pACYC184 backbone were then incubated with 1 μl of USER enzyme at room temperature for 15 min before being transformed into E. coli cloning strain C3019 (NEB) to generate the ribAp-lacZ -pACYC184 reporter plasmid.

    Transformation Assay:

    Article Title: Characterization of Transcription Factors That Regulate the Type IV Secretion System and Riboflavin Biosynthesis in Wolbachia of Brugia malayi
    Article Snippet: The ribAp-lacZ fusion was amplified with primers acggagacaUCGCTCTGCCGGTGGTTACCA and agggaaagUAACCTATAAAAATAGGCGTATCACGAGG derived from the ribAp-lacZ -pNK1415 construct. .. 10 μl of the ribAp-lacZ insert and 1 μl of the pACYC184 backbone were then incubated with 1 μl of USER enzyme at room temperature for 15 min before being transformed into E. coli cloning strain C3019 (NEB) to generate the ribAp-lacZ -pACYC184 reporter plasmid. .. Both ribAp-lacZ -pACYC184 and wBmxR1- pET21a or wBmxR2 -pET21a were transformed into E. coli strain C2566 (NEB) for measuring β-galactosidase activity measurement.

    Plasmid Preparation:

    Article Title: Characterization of Transcription Factors That Regulate the Type IV Secretion System and Riboflavin Biosynthesis in Wolbachia of Brugia malayi
    Article Snippet: The ribAp-lacZ fusion was amplified with primers acggagacaUCGCTCTGCCGGTGGTTACCA and agggaaagUAACCTATAAAAATAGGCGTATCACGAGG derived from the ribAp-lacZ -pNK1415 construct. .. 10 μl of the ribAp-lacZ insert and 1 μl of the pACYC184 backbone were then incubated with 1 μl of USER enzyme at room temperature for 15 min before being transformed into E. coli cloning strain C3019 (NEB) to generate the ribAp-lacZ -pACYC184 reporter plasmid. .. Both ribAp-lacZ -pACYC184 and wBmxR1- pET21a or wBmxR2 -pET21a were transformed into E. coli strain C2566 (NEB) for measuring β-galactosidase activity measurement.

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