cyclopiazonic acid  (Alomone Labs)


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    Name:
    Cyclopiazonic Acid
    Description:
    Cyclopiazonic Acid is a cell permeable reversible inhibitor of sarcoplasmic reticulum Ca2 ATPase
    Catalog Number:
    C-750
    Price:
    176.0
    Category:
    Small Molecule
    Source:
    Penicillium griseofulvum.
    Applications:
    0
    Purity:
    >99% (HPLC)
    Size:
    5 mg
    Format:
    Lyophilized/solid.
    Formula:
    C20H20N2O3
    Molecular Weight:
    336.4
    Molecule Name:
    (6aR,11aS,11bR)-rel-10-Acetyl-2,6,6a,7,11a,11b,hexahydro-7,7-dimethyl-9H-pyrrolo[1',2':2,3]isoindolo[4,5,6-cd] indol-9-one.
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    Structured Review

    Alomone Labs cyclopiazonic acid
    Cyclopiazonic Acid
    Cyclopiazonic Acid is a cell permeable reversible inhibitor of sarcoplasmic reticulum Ca2 ATPase
    https://www.bioz.com/result/cyclopiazonic acid/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cyclopiazonic acid - by Bioz Stars, 2021-09
    93/100 stars

    Images

    1) Product Images from "Calcium store-mediated signaling in sustentacular cells of the mouse olfactory epithelium"

    Article Title: Calcium store-mediated signaling in sustentacular cells of the mouse olfactory epithelium

    Journal: Glia

    doi: 10.1002/glia.20792

    Purinergic evoked calcium transients are mediated via intracellular calcium release Slices were superfused with multiple applications of 10 μM UTP in 5 min. acute (A) or 15–30 min. chronic (B,C) presence of 10 μM cyclopiazonic acid (CPA) or vehicle (0.1% DMSO). (▲ indicates time of UTP superfusion, □ indicates time of drug superfusion). Right panels: Average peak calcium transient amplitudes are shown * , p
    Figure Legend Snippet: Purinergic evoked calcium transients are mediated via intracellular calcium release Slices were superfused with multiple applications of 10 μM UTP in 5 min. acute (A) or 15–30 min. chronic (B,C) presence of 10 μM cyclopiazonic acid (CPA) or vehicle (0.1% DMSO). (▲ indicates time of UTP superfusion, □ indicates time of drug superfusion). Right panels: Average peak calcium transient amplitudes are shown * , p

    Techniques Used:

    Mechanism of G-protein coupled receptor calcium mobilization Activation of GPCRs activates phospholipase C and production of DAG and IP 3 . IP 3 activates the IP 3 receptor with subsequent release of calcium from stores. The increase in cytosolic calcium induces calcium-dependent calcium release via the ryanodine receptor. Large arrows depict pathway of GPCR activation, and thin lines depict pharmacological targets. Abbreviations: 2-APB, 2-aminoethoxydiphenyl borate; ATPase, Ca 2+ -ATPase; CPA, cyclopiazonic acid; DAG, diacylglycerol; ER, endoplasmic reticulum; GPCR, G-protein coupled receptor; G, G protein; IP 3 R, IP 3 receptor; PLC, phospholipase C; PIP 2 , phosphoinositols; RyR, ryanodine receptor.
    Figure Legend Snippet: Mechanism of G-protein coupled receptor calcium mobilization Activation of GPCRs activates phospholipase C and production of DAG and IP 3 . IP 3 activates the IP 3 receptor with subsequent release of calcium from stores. The increase in cytosolic calcium induces calcium-dependent calcium release via the ryanodine receptor. Large arrows depict pathway of GPCR activation, and thin lines depict pharmacological targets. Abbreviations: 2-APB, 2-aminoethoxydiphenyl borate; ATPase, Ca 2+ -ATPase; CPA, cyclopiazonic acid; DAG, diacylglycerol; ER, endoplasmic reticulum; GPCR, G-protein coupled receptor; G, G protein; IP 3 R, IP 3 receptor; PLC, phospholipase C; PIP 2 , phosphoinositols; RyR, ryanodine receptor.

    Techniques Used: Activation Assay, Planar Chromatography

    2) Product Images from "Ca2+-sparks constitute elementary building blocks for global Ca2+-signals in myocytes of retinal arterioles"

    Article Title: Ca2+-sparks constitute elementary building blocks for global Ca2+-signals in myocytes of retinal arterioles

    Journal: Cell Calcium

    doi: 10.1016/j.ceca.2006.08.005

    More prolonged application of cyclopiazonic acid inhibited both Ca 2+ -sparks and oscillations. (A) Summary bar chart for 22 cells showing spark frequency under control conditions and 30–60 s after start of exposure to cyclopiazonic acid. (B) Summary data for Ca 2+ -oscillations in the same experiments, showing changes in frequency, amplitude and full duration at half-maximum. Data was averaged over 15 s intervals ( * P
    Figure Legend Snippet: More prolonged application of cyclopiazonic acid inhibited both Ca 2+ -sparks and oscillations. (A) Summary bar chart for 22 cells showing spark frequency under control conditions and 30–60 s after start of exposure to cyclopiazonic acid. (B) Summary data for Ca 2+ -oscillations in the same experiments, showing changes in frequency, amplitude and full duration at half-maximum. Data was averaged over 15 s intervals ( * P

    Techniques Used:

    Cyclopiazonic acid, nifedipine and low external [Ca 2+ ] reduced but did not abolish caffeine transients. (A) Increases in fura2 fluorescence ratio (Δ R ) evoked by 3 serial applications of caffeine (10 mM) in 6 different arterioles. The first 2 applications (1 and 2) were made under control conditions and produced similar responses. The third application (3) was made after 40 s superfusion with cyclopiazonic acid (20 μM), and this response was reduced in each case. (B) Similar plots for 6 arterioles comparing 2 control applications of caffeine with a third application after 40 s exposure to nifedipine (10 μM). (C) Similar plots for 6 arterioles comparing 2 control applications of caffeine with a third application after 40 s exposure to low external [Ca 2+ ].
    Figure Legend Snippet: Cyclopiazonic acid, nifedipine and low external [Ca 2+ ] reduced but did not abolish caffeine transients. (A) Increases in fura2 fluorescence ratio (Δ R ) evoked by 3 serial applications of caffeine (10 mM) in 6 different arterioles. The first 2 applications (1 and 2) were made under control conditions and produced similar responses. The third application (3) was made after 40 s superfusion with cyclopiazonic acid (20 μM), and this response was reduced in each case. (B) Similar plots for 6 arterioles comparing 2 control applications of caffeine with a third application after 40 s exposure to nifedipine (10 μM). (C) Similar plots for 6 arterioles comparing 2 control applications of caffeine with a third application after 40 s exposure to low external [Ca 2+ ].

    Techniques Used: Fluorescence, Produced

    Cyclopiazonic acid initially increased the amplitude of spontaneous Ca 2+ -oscillations. (A) Linescan and normalized fluorescence plot for an arteriolar myocyte under control conditions and during superfusion with cyclopiazonic acid (20 μM). (B) Summary data (mean + S.E.M.) from 35 cells for 4 consecutive 15 s periods from 30 s before to 30 s after the start of superfusion with cyclopiazonic acid ( * P
    Figure Legend Snippet: Cyclopiazonic acid initially increased the amplitude of spontaneous Ca 2+ -oscillations. (A) Linescan and normalized fluorescence plot for an arteriolar myocyte under control conditions and during superfusion with cyclopiazonic acid (20 μM). (B) Summary data (mean + S.E.M.) from 35 cells for 4 consecutive 15 s periods from 30 s before to 30 s after the start of superfusion with cyclopiazonic acid ( * P

    Techniques Used: Fluorescence

    Cyclopiazonic acid initially increased Ca 2+ -spark frequency. (A) Linescan and graph showing control activity in a single myocyte within an arteriole under control conditions, and during the first 45 s of superfusion with 20 μM cyclopiazonic acid. Time-course data in the graph refers to the indicated region of interest (R.O.I.). (B) Summary data from 32 cells for, (i) spark frequency, and (ii) spark amplitude during 3 consecutive, 15 s periods ( * P
    Figure Legend Snippet: Cyclopiazonic acid initially increased Ca 2+ -spark frequency. (A) Linescan and graph showing control activity in a single myocyte within an arteriole under control conditions, and during the first 45 s of superfusion with 20 μM cyclopiazonic acid. Time-course data in the graph refers to the indicated region of interest (R.O.I.). (B) Summary data from 32 cells for, (i) spark frequency, and (ii) spark amplitude during 3 consecutive, 15 s periods ( * P

    Techniques Used: Activity Assay

    3) Product Images from "Calcium store-mediated signaling in sustentacular cells of the mouse olfactory epithelium"

    Article Title: Calcium store-mediated signaling in sustentacular cells of the mouse olfactory epithelium

    Journal: Glia

    doi: 10.1002/glia.20792

    Purinergic evoked calcium transients are mediated via intracellular calcium release Slices were superfused with multiple applications of 10 μM UTP in 5 min. acute (A) or 15–30 min. chronic (B,C) presence of 10 μM cyclopiazonic acid (CPA) or vehicle (0.1% DMSO). (▲ indicates time of UTP superfusion, □ indicates time of drug superfusion). Right panels: Average peak calcium transient amplitudes are shown * , p
    Figure Legend Snippet: Purinergic evoked calcium transients are mediated via intracellular calcium release Slices were superfused with multiple applications of 10 μM UTP in 5 min. acute (A) or 15–30 min. chronic (B,C) presence of 10 μM cyclopiazonic acid (CPA) or vehicle (0.1% DMSO). (▲ indicates time of UTP superfusion, □ indicates time of drug superfusion). Right panels: Average peak calcium transient amplitudes are shown * , p

    Techniques Used:

    Mechanism of G-protein coupled receptor calcium mobilization Activation of GPCRs activates phospholipase C and production of DAG and IP 3 . IP 3 activates the IP 3 receptor with subsequent release of calcium from stores. The increase in cytosolic calcium induces calcium-dependent calcium release via the ryanodine receptor. Large arrows depict pathway of GPCR activation, and thin lines depict pharmacological targets. Abbreviations: 2-APB, 2-aminoethoxydiphenyl borate; ATPase, Ca 2+ -ATPase; CPA, cyclopiazonic acid; DAG, diacylglycerol; ER, endoplasmic reticulum; GPCR, G-protein coupled receptor; G, G protein; IP 3 R, IP 3 receptor; PLC, phospholipase C; PIP 2 , phosphoinositols; RyR, ryanodine receptor.
    Figure Legend Snippet: Mechanism of G-protein coupled receptor calcium mobilization Activation of GPCRs activates phospholipase C and production of DAG and IP 3 . IP 3 activates the IP 3 receptor with subsequent release of calcium from stores. The increase in cytosolic calcium induces calcium-dependent calcium release via the ryanodine receptor. Large arrows depict pathway of GPCR activation, and thin lines depict pharmacological targets. Abbreviations: 2-APB, 2-aminoethoxydiphenyl borate; ATPase, Ca 2+ -ATPase; CPA, cyclopiazonic acid; DAG, diacylglycerol; ER, endoplasmic reticulum; GPCR, G-protein coupled receptor; G, G protein; IP 3 R, IP 3 receptor; PLC, phospholipase C; PIP 2 , phosphoinositols; RyR, ryanodine receptor.

    Techniques Used: Activation Assay, Planar Chromatography

    4) Product Images from "Calcium store-mediated signaling in sustentacular cells of the mouse olfactory epithelium"

    Article Title: Calcium store-mediated signaling in sustentacular cells of the mouse olfactory epithelium

    Journal: Glia

    doi: 10.1002/glia.20792

    Purinergic evoked calcium transients are mediated via intracellular calcium release Slices were superfused with multiple applications of 10 μM UTP in 5 min. acute (A) or 15–30 min. chronic (B,C) presence of 10 μM cyclopiazonic acid (CPA) or vehicle (0.1% DMSO). (▲ indicates time of UTP superfusion, □ indicates time of drug superfusion). Right panels: Average peak calcium transient amplitudes are shown * , p
    Figure Legend Snippet: Purinergic evoked calcium transients are mediated via intracellular calcium release Slices were superfused with multiple applications of 10 μM UTP in 5 min. acute (A) or 15–30 min. chronic (B,C) presence of 10 μM cyclopiazonic acid (CPA) or vehicle (0.1% DMSO). (▲ indicates time of UTP superfusion, □ indicates time of drug superfusion). Right panels: Average peak calcium transient amplitudes are shown * , p

    Techniques Used:

    Mechanism of G-protein coupled receptor calcium mobilization Activation of GPCRs activates phospholipase C and production of DAG and IP 3 . IP 3 activates the IP 3 receptor with subsequent release of calcium from stores. The increase in cytosolic calcium induces calcium-dependent calcium release via the ryanodine receptor. Large arrows depict pathway of GPCR activation, and thin lines depict pharmacological targets. Abbreviations: 2-APB, 2-aminoethoxydiphenyl borate; ATPase, Ca 2+ -ATPase; CPA, cyclopiazonic acid; DAG, diacylglycerol; ER, endoplasmic reticulum; GPCR, G-protein coupled receptor; G, G protein; IP 3 R, IP 3 receptor; PLC, phospholipase C; PIP 2 , phosphoinositols; RyR, ryanodine receptor.
    Figure Legend Snippet: Mechanism of G-protein coupled receptor calcium mobilization Activation of GPCRs activates phospholipase C and production of DAG and IP 3 . IP 3 activates the IP 3 receptor with subsequent release of calcium from stores. The increase in cytosolic calcium induces calcium-dependent calcium release via the ryanodine receptor. Large arrows depict pathway of GPCR activation, and thin lines depict pharmacological targets. Abbreviations: 2-APB, 2-aminoethoxydiphenyl borate; ATPase, Ca 2+ -ATPase; CPA, cyclopiazonic acid; DAG, diacylglycerol; ER, endoplasmic reticulum; GPCR, G-protein coupled receptor; G, G protein; IP 3 R, IP 3 receptor; PLC, phospholipase C; PIP 2 , phosphoinositols; RyR, ryanodine receptor.

    Techniques Used: Activation Assay, Planar Chromatography

    Related Articles

    other:

    Article Title: α-Synuclein oligomers mediate the aberrant form of spike-induced calcium release from IP3 receptor
    Article Snippet: Drugs used Depending on the purpose of the electrophysiological experiments, the chemicals applied via the recording solution included nifedipine (10 μM), ω-conotoxin (1 μM), ω-agatoxin (50 nM), apamin (100 nM), paxilline (10 μM), cyclopiazonic acid (CPA; 30 μM; all purchased from Alomone Labs, Jerusalem, Israel), BAPTA-AM (1 0 μM; Sigma), or U73122 (4 μM; Sigma).

    Article Title: Calcium-induced calcium release and type 3 ryanodine receptors modulate the slow afterhyperpolarising current, sIAHP, and its potentiation in hippocampal pyramidal neurons
    Article Snippet: Drugs and solutions Ryanodine was obtained from Calbiochem (Millipore, Heartfordshire, UK) and Alomone Labs (Israel); cyclopiazonic acid (CPA) from Alomone Labs (Israel); caffeine from Calbiochem (Millipore, Heartfordshire, UK); KMeSO4 from Fisher Scientific (Loughborough, UK); tetrodotoxin (TTX) citrate-free from Latoxan (Rosans, France); d-tubocurarine hydrochloride (dTC), Na2 ATP, Na3GTP and tetraethylammonium (TEA) from Sigma-Aldrich (Dorset, UK); (Rp)-Adenosine-3`,5`-monophosphorothioate (Rp-cAMPS) (BioLog Life Science Institute, Germany).

    Article Title: A novel mutation in KCNK16 causing a gain-of-function in the TALK-1 potassium channel: a new cause of maturity onset diabetes of the young
    Article Snippet: For endoplasmic reticulum (ER) Ca2+ [Ca2+ ER ]: Islets were perfused in Krebs-Ringer–HEPES buffer without extracellular Ca2+ and 100 μM diazoxide and monitored for Ca2+ ER release mediated through blockade of the sarco(endo)plasmic reticulum Ca2+ -ATPase (SERCA) with 50 μM cyclopiazonic acid (CPA, Alomone labs), as previously described .

    Article Title: Diurnal properties of voltage-gated Ca2+ currents in suprachiasmatic nucleus and roles in action potential firing
    Article Snippet: Drugs were used at final concentrations of 1 μM tetrodotoxin (TTX; Alomone Labs, Jerusalem, Israel; T-550), 10 μM Nimodipine (Alomone Labs, Jerusalem, Israel; N150), 10 μM dantrolene (Dan; Sigma, Darmstadt, Germany; D9175), 1 μM cyclopiazonic acid (CPA; Alomone Labs, Jerusalem, Israel; C-750), 3 μM ω-conotoxin GVIA (ConoGVIA; Alomone Labs, Jerusalem, Israel; C-300), 3 μM ω-conotoxin MVIIC (ConoMVIIC; Alomone Labs, Jerusalem, Israel; C-150), 200 nM ω-agatoxin IVA (AgaIVA;Alomone Labs, Jerusalem, Israel; STA-500), 1 μM TTA-P2, (TTAP2;Alomone Labs, Jerusalem, Israel; T-155), 200 μM CdCl2 (Cd2+ ; Sigma; 529575), 30 μM NiCl2 (Ni2+ ; Sigma; 22387), and 5 μM Bay K8644 (Bay K; Sigma, Darmstadt, Germany; B133).

    Article Title: Glucagon-like peptide-1 receptor pathway inhibits extracellular matrix production by mesangial cells through store-operated Ca2+channel
    Article Snippet: Cyclopiazonic acid (CPA) was purchased from Alomone Labs (Cat no: C-750 and Lot no: C750CS1025).

    Blocking Assay:

    Article Title: Diffusion of Ca2+ from Small Boutons en Passant into the Axon Shapes AP-Evoked Ca2+ Transients
    Article Snippet: .. To block the sarco/endoplasmic reticulum Ca2+ -ATPase (SERCA), cyclopiazonic acid (CPA, 25 μ M; Alomone Labs, Jerusalem, Israel) was added to the superfusate for ≥10 min. ..

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  • 93
    Alomone Labs cyclopiazonic acid
    Purinergic evoked calcium transients are mediated via intracellular calcium release Slices were superfused with multiple applications of 10 μM UTP in 5 min. acute (A) or 15–30 min. chronic (B,C) presence of 10 μM <t>cyclopiazonic</t> acid (CPA) or vehicle (0.1% DMSO). (▲ indicates time of UTP superfusion, □ indicates time of drug superfusion). Right panels: Average peak calcium transient amplitudes are shown * , p
    Cyclopiazonic Acid, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cyclopiazonic acid/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cyclopiazonic acid - by Bioz Stars, 2021-09
    93/100 stars
      Buy from Supplier

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    Purinergic evoked calcium transients are mediated via intracellular calcium release Slices were superfused with multiple applications of 10 μM UTP in 5 min. acute (A) or 15–30 min. chronic (B,C) presence of 10 μM cyclopiazonic acid (CPA) or vehicle (0.1% DMSO). (▲ indicates time of UTP superfusion, □ indicates time of drug superfusion). Right panels: Average peak calcium transient amplitudes are shown * , p

    Journal: Glia

    Article Title: Calcium store-mediated signaling in sustentacular cells of the mouse olfactory epithelium

    doi: 10.1002/glia.20792

    Figure Lengend Snippet: Purinergic evoked calcium transients are mediated via intracellular calcium release Slices were superfused with multiple applications of 10 μM UTP in 5 min. acute (A) or 15–30 min. chronic (B,C) presence of 10 μM cyclopiazonic acid (CPA) or vehicle (0.1% DMSO). (▲ indicates time of UTP superfusion, □ indicates time of drug superfusion). Right panels: Average peak calcium transient amplitudes are shown * , p

    Article Snippet: All chemicals were obtained from Sigma Aldrich Chemicals (St. Louis, MO) except for ryanodine (Calbiochem, San Diego, CA), cyclopiazonic acid (CPA, Alomone Labs, Jerusalem), and 2-aminoethoxydiphenyl borate (2-APB, Caymen Chemical, Ann Arbor, MI).

    Techniques:

    Mechanism of G-protein coupled receptor calcium mobilization Activation of GPCRs activates phospholipase C and production of DAG and IP 3 . IP 3 activates the IP 3 receptor with subsequent release of calcium from stores. The increase in cytosolic calcium induces calcium-dependent calcium release via the ryanodine receptor. Large arrows depict pathway of GPCR activation, and thin lines depict pharmacological targets. Abbreviations: 2-APB, 2-aminoethoxydiphenyl borate; ATPase, Ca 2+ -ATPase; CPA, cyclopiazonic acid; DAG, diacylglycerol; ER, endoplasmic reticulum; GPCR, G-protein coupled receptor; G, G protein; IP 3 R, IP 3 receptor; PLC, phospholipase C; PIP 2 , phosphoinositols; RyR, ryanodine receptor.

    Journal: Glia

    Article Title: Calcium store-mediated signaling in sustentacular cells of the mouse olfactory epithelium

    doi: 10.1002/glia.20792

    Figure Lengend Snippet: Mechanism of G-protein coupled receptor calcium mobilization Activation of GPCRs activates phospholipase C and production of DAG and IP 3 . IP 3 activates the IP 3 receptor with subsequent release of calcium from stores. The increase in cytosolic calcium induces calcium-dependent calcium release via the ryanodine receptor. Large arrows depict pathway of GPCR activation, and thin lines depict pharmacological targets. Abbreviations: 2-APB, 2-aminoethoxydiphenyl borate; ATPase, Ca 2+ -ATPase; CPA, cyclopiazonic acid; DAG, diacylglycerol; ER, endoplasmic reticulum; GPCR, G-protein coupled receptor; G, G protein; IP 3 R, IP 3 receptor; PLC, phospholipase C; PIP 2 , phosphoinositols; RyR, ryanodine receptor.

    Article Snippet: All chemicals were obtained from Sigma Aldrich Chemicals (St. Louis, MO) except for ryanodine (Calbiochem, San Diego, CA), cyclopiazonic acid (CPA, Alomone Labs, Jerusalem), and 2-aminoethoxydiphenyl borate (2-APB, Caymen Chemical, Ann Arbor, MI).

    Techniques: Activation Assay, Planar Chromatography

    More prolonged application of cyclopiazonic acid inhibited both Ca 2+ -sparks and oscillations. (A) Summary bar chart for 22 cells showing spark frequency under control conditions and 30–60 s after start of exposure to cyclopiazonic acid. (B) Summary data for Ca 2+ -oscillations in the same experiments, showing changes in frequency, amplitude and full duration at half-maximum. Data was averaged over 15 s intervals ( * P

    Journal: Cell Calcium

    Article Title: Ca2+-sparks constitute elementary building blocks for global Ca2+-signals in myocytes of retinal arterioles

    doi: 10.1016/j.ceca.2006.08.005

    Figure Lengend Snippet: More prolonged application of cyclopiazonic acid inhibited both Ca 2+ -sparks and oscillations. (A) Summary bar chart for 22 cells showing spark frequency under control conditions and 30–60 s after start of exposure to cyclopiazonic acid. (B) Summary data for Ca 2+ -oscillations in the same experiments, showing changes in frequency, amplitude and full duration at half-maximum. Data was averaged over 15 s intervals ( * P

    Article Snippet: The drug suppliers and bath concentrations of vehicle (%v/v) were as follows: cyclopiazonic acid (Alexis; 0.05% DMSO); ryanodine (Alomone; 0.1% ethanol), tetracaine (Sigma–Aldrich; 0.01% H2O), nifedipine (Sigma–Aldrich; 0.01% ethanol), EGTA (Lancaster; dissolved directly in bath solution).

    Techniques:

    Cyclopiazonic acid, nifedipine and low external [Ca 2+ ] reduced but did not abolish caffeine transients. (A) Increases in fura2 fluorescence ratio (Δ R ) evoked by 3 serial applications of caffeine (10 mM) in 6 different arterioles. The first 2 applications (1 and 2) were made under control conditions and produced similar responses. The third application (3) was made after 40 s superfusion with cyclopiazonic acid (20 μM), and this response was reduced in each case. (B) Similar plots for 6 arterioles comparing 2 control applications of caffeine with a third application after 40 s exposure to nifedipine (10 μM). (C) Similar plots for 6 arterioles comparing 2 control applications of caffeine with a third application after 40 s exposure to low external [Ca 2+ ].

    Journal: Cell Calcium

    Article Title: Ca2+-sparks constitute elementary building blocks for global Ca2+-signals in myocytes of retinal arterioles

    doi: 10.1016/j.ceca.2006.08.005

    Figure Lengend Snippet: Cyclopiazonic acid, nifedipine and low external [Ca 2+ ] reduced but did not abolish caffeine transients. (A) Increases in fura2 fluorescence ratio (Δ R ) evoked by 3 serial applications of caffeine (10 mM) in 6 different arterioles. The first 2 applications (1 and 2) were made under control conditions and produced similar responses. The third application (3) was made after 40 s superfusion with cyclopiazonic acid (20 μM), and this response was reduced in each case. (B) Similar plots for 6 arterioles comparing 2 control applications of caffeine with a third application after 40 s exposure to nifedipine (10 μM). (C) Similar plots for 6 arterioles comparing 2 control applications of caffeine with a third application after 40 s exposure to low external [Ca 2+ ].

    Article Snippet: The drug suppliers and bath concentrations of vehicle (%v/v) were as follows: cyclopiazonic acid (Alexis; 0.05% DMSO); ryanodine (Alomone; 0.1% ethanol), tetracaine (Sigma–Aldrich; 0.01% H2O), nifedipine (Sigma–Aldrich; 0.01% ethanol), EGTA (Lancaster; dissolved directly in bath solution).

    Techniques: Fluorescence, Produced

    Cyclopiazonic acid initially increased the amplitude of spontaneous Ca 2+ -oscillations. (A) Linescan and normalized fluorescence plot for an arteriolar myocyte under control conditions and during superfusion with cyclopiazonic acid (20 μM). (B) Summary data (mean + S.E.M.) from 35 cells for 4 consecutive 15 s periods from 30 s before to 30 s after the start of superfusion with cyclopiazonic acid ( * P

    Journal: Cell Calcium

    Article Title: Ca2+-sparks constitute elementary building blocks for global Ca2+-signals in myocytes of retinal arterioles

    doi: 10.1016/j.ceca.2006.08.005

    Figure Lengend Snippet: Cyclopiazonic acid initially increased the amplitude of spontaneous Ca 2+ -oscillations. (A) Linescan and normalized fluorescence plot for an arteriolar myocyte under control conditions and during superfusion with cyclopiazonic acid (20 μM). (B) Summary data (mean + S.E.M.) from 35 cells for 4 consecutive 15 s periods from 30 s before to 30 s after the start of superfusion with cyclopiazonic acid ( * P

    Article Snippet: The drug suppliers and bath concentrations of vehicle (%v/v) were as follows: cyclopiazonic acid (Alexis; 0.05% DMSO); ryanodine (Alomone; 0.1% ethanol), tetracaine (Sigma–Aldrich; 0.01% H2O), nifedipine (Sigma–Aldrich; 0.01% ethanol), EGTA (Lancaster; dissolved directly in bath solution).

    Techniques: Fluorescence

    Cyclopiazonic acid initially increased Ca 2+ -spark frequency. (A) Linescan and graph showing control activity in a single myocyte within an arteriole under control conditions, and during the first 45 s of superfusion with 20 μM cyclopiazonic acid. Time-course data in the graph refers to the indicated region of interest (R.O.I.). (B) Summary data from 32 cells for, (i) spark frequency, and (ii) spark amplitude during 3 consecutive, 15 s periods ( * P

    Journal: Cell Calcium

    Article Title: Ca2+-sparks constitute elementary building blocks for global Ca2+-signals in myocytes of retinal arterioles

    doi: 10.1016/j.ceca.2006.08.005

    Figure Lengend Snippet: Cyclopiazonic acid initially increased Ca 2+ -spark frequency. (A) Linescan and graph showing control activity in a single myocyte within an arteriole under control conditions, and during the first 45 s of superfusion with 20 μM cyclopiazonic acid. Time-course data in the graph refers to the indicated region of interest (R.O.I.). (B) Summary data from 32 cells for, (i) spark frequency, and (ii) spark amplitude during 3 consecutive, 15 s periods ( * P

    Article Snippet: The drug suppliers and bath concentrations of vehicle (%v/v) were as follows: cyclopiazonic acid (Alexis; 0.05% DMSO); ryanodine (Alomone; 0.1% ethanol), tetracaine (Sigma–Aldrich; 0.01% H2O), nifedipine (Sigma–Aldrich; 0.01% ethanol), EGTA (Lancaster; dissolved directly in bath solution).

    Techniques: Activity Assay