chelerytrine  (Alomone Labs)


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  • 92
    Name:
    Chelerythrine chloride
    Description:
    Chelerythrine chloride is a potent cell permeable inhibitor of protein kinase C PKC It is at least 100 fold more selective for PKCs than for other kinases
    Catalog Number:
    C-400
    Price:
    173.0
    Category:
    Small Molecule
    Source:
    Macleaya Cordata.
    Applications:
    0
    Purity:
    >99%
    Size:
    1 Vials containing 1 mg each
    Format:
    Lyophilized/solid.
    Formula:
    C21H18ClNO4
    Molecular Weight:
    383.84
    Molecule Name:
    1,2-Dimethoxy-12-methyl[1,3]benzodioxolo[5,6-c]phenanthridinium chloride.
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    Structured Review

    Alomone Labs chelerytrine
    Chelerythrine chloride
    Chelerythrine chloride is a potent cell permeable inhibitor of protein kinase C PKC It is at least 100 fold more selective for PKCs than for other kinases
    https://www.bioz.com/result/chelerytrine/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    chelerytrine - by Bioz Stars, 2021-09
    92/100 stars

    Images

    1) Product Images from "Opposed Actions of PKA Isozymes (RI and RII) and PKC Isoforms (cPKCβI and nPKCε) in Neuromuscular Developmental Synapse Elimination"

    Article Title: Opposed Actions of PKA Isozymes (RI and RII) and PKC Isoforms (cPKCβI and nPKCε) in Neuromuscular Developmental Synapse Elimination

    Journal: Cells

    doi: 10.3390/cells8111304

    PKC activity modulation in axonal loss. ( A ) shows the percentage of singly-, doubly-, and triply- (or more) innervated NMJs in the control mice (PBS) and after four applications of one of the following substances: the PKC paninhibitors Chelerytrine (CHE) and CaC, and the PKC panstimulators (BRY, at 1 and 10 nM) and PMA. CHE and CaC action results in the persistence of many polyinnervated synapses. Accordingly, both PKC stimulators PMA and BRY increase the number of monoinnervated junctions and clearly decrease the percentage of doubly-innervated junctions in the case of BRY. ( B ) shows the percentage of singly-, doubly-, and triply (or more) innervated NMJs after exposure to the cPKCβI and nPKCε isoform selective inhibitors βIV 5–3 and εV 1–2 , and the cPKCβI and nPKCε selective activators dPPA and FR 236,924 respectively. The selective inhibitors similarly increase the doubly- and triply-innervated synapses with the corresponding reduction in the monoinnervated junctions. The activators considerably accelerate nerve terminal elimination. Data were presented as percentages of NMJ ± SD (for each treatment and PBS control: n pups = 6–9; n = 11–18 LALs; n NMJ: PBS: 2538; Bry 1 nM: 1232 Bry 10 nM:1384; PMA:1247; CaC: 1573; Che:1499; βIV 5–3 : 1275; dPPA: 1121; εV 1–2 :1663 and FR 236924: 1367). Fisher’s test: * p
    Figure Legend Snippet: PKC activity modulation in axonal loss. ( A ) shows the percentage of singly-, doubly-, and triply- (or more) innervated NMJs in the control mice (PBS) and after four applications of one of the following substances: the PKC paninhibitors Chelerytrine (CHE) and CaC, and the PKC panstimulators (BRY, at 1 and 10 nM) and PMA. CHE and CaC action results in the persistence of many polyinnervated synapses. Accordingly, both PKC stimulators PMA and BRY increase the number of monoinnervated junctions and clearly decrease the percentage of doubly-innervated junctions in the case of BRY. ( B ) shows the percentage of singly-, doubly-, and triply (or more) innervated NMJs after exposure to the cPKCβI and nPKCε isoform selective inhibitors βIV 5–3 and εV 1–2 , and the cPKCβI and nPKCε selective activators dPPA and FR 236,924 respectively. The selective inhibitors similarly increase the doubly- and triply-innervated synapses with the corresponding reduction in the monoinnervated junctions. The activators considerably accelerate nerve terminal elimination. Data were presented as percentages of NMJ ± SD (for each treatment and PBS control: n pups = 6–9; n = 11–18 LALs; n NMJ: PBS: 2538; Bry 1 nM: 1232 Bry 10 nM:1384; PMA:1247; CaC: 1573; Che:1499; βIV 5–3 : 1275; dPPA: 1121; εV 1–2 :1663 and FR 236924: 1367). Fisher’s test: * p

    Techniques Used: Activity Assay, Mouse Assay

    Related Articles

    other:

    Article Title: Opposed Actions of PKA Isozymes (RI and RII) and PKC Isoforms (cPKCβI and nPKCε) in Neuromuscular Developmental Synapse Elimination
    Article Snippet: Selective PKC Substances Antagonists : The stock solutions were Chelerytrine (CHE, C-400, Alomone, Jerusalem, Israel) 10 mM; Calfostein C (CaC, C6303, Sigma-Aldrich) 2.5 mM; Peptide βIV 5–3 (βIV 5–3 Mochly Rosen, Standford University) 10 mM; Peptide εV 1–2 , (εV 1–2, 539522.

    Article Title: Upregulation of voltage-gated Na+ channels by long-term activation of the ghrelin-growth hormone secretagogue receptor in clonal GC somatotropes.
    Article Snippet: A central question in adenohypophyseal cell physiology concerns the role of transmembrane ionic fluxes in the initiation of the hormone secretion process.. In the current report, we investigated the effects of the growth hormone (GH) secretagogues ghrelin and GH-releasing peptide-6 (GHRP-6) on the regulation of the functional expression of voltage-gated Na(+) channels using the tumoral somatotrope GC cell line as a model.

    Cell Culture:

    Article Title: Cisplatin-induced neuropathic pain is mediated by upregulation of N-type voltage-gated calcium channels in dorsal root ganglion neurons.
    Article Snippet: .. Cisplatin is important in the treatment of various types of cancer.. Although it is highly effective, it also has severe side effects, with neurotoxicity in dorsal root ganglion (DRG) neurons being one of the most common. ..

    Incubation:

    Article Title: Cisplatin-induced neuropathic pain is mediated by upregulation of N-type voltage-gated calcium channels in dorsal root ganglion neurons.
    Article Snippet: .. Cisplatin is important in the treatment of various types of cancer.. Although it is highly effective, it also has severe side effects, with neurotoxicity in dorsal root ganglion (DRG) neurons being one of the most common. ..

    Blocking Assay:

    Article Title: Corticotropin-releasing factor increases Purkinje neuron excitability by modulating sodium, potassium, and Ih currents
    Article Snippet: .. Chelerythrine (10 μM) (Alomone Labs) was added externally to the bath to block the PKC pathway. ..

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  • 80
    Alomone Labs anti cav γ4
    Ca V <t>γ4</t> is downregulated by silencing of the transcription factor MafA. a Rank of Pearson correlation coefficient ( R ) (tested by t -test) calculated by mRNA expression (Microarray, human islets) between Ca V γ4 ( CACNG4 ) and the transcription factors known for pancreas development. n = 128 donors. b Ca V γ4 mRNA expression in Pdx1, NeuroD1, MafA, Isl1, or Tcf7l2 silenced INS-1 cells. n = 3, ** p = 0.001 (NeuroD1), *** p
    Anti Cav γ4, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cav γ4/product/Alomone Labs
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cav γ4 - by Bioz Stars, 2021-09
    80/100 stars
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    94
    Alomone Labs rabbit anti trpa1
    WIRS induced up-regulated protein expression of <t>TRPA1</t> in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p
    Rabbit Anti Trpa1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti trpa1/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti trpa1 - by Bioz Stars, 2021-09
    94/100 stars
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    92
    Alomone Labs chelerytrine
    PKC activity modulation in axonal loss. ( A ) shows the percentage of singly-, doubly-, and triply- (or more) innervated NMJs in the control mice (PBS) and after four applications of one of the following substances: the PKC paninhibitors <t>Chelerytrine</t> (CHE) and CaC, and the PKC panstimulators (BRY, at 1 and 10 nM) and PMA. CHE and CaC action results in the persistence of many polyinnervated synapses. Accordingly, both PKC stimulators PMA and BRY increase the number of monoinnervated junctions and clearly decrease the percentage of doubly-innervated junctions in the case of BRY. ( B ) shows the percentage of singly-, doubly-, and triply (or more) innervated NMJs after exposure to the cPKCβI and nPKCε isoform selective inhibitors βIV 5–3 and εV 1–2 , and the cPKCβI and nPKCε selective activators dPPA and FR 236,924 respectively. The selective inhibitors similarly increase the doubly- and triply-innervated synapses with the corresponding reduction in the monoinnervated junctions. The activators considerably accelerate nerve terminal elimination. Data were presented as percentages of NMJ ± SD (for each treatment and PBS control: n pups = 6–9; n = 11–18 LALs; n NMJ: PBS: 2538; Bry 1 nM: 1232 Bry 10 nM:1384; PMA:1247; CaC: 1573; Che:1499; βIV 5–3 : 1275; dPPA: 1121; εV 1–2 :1663 and FR 236924: 1367). Fisher’s test: * p
    Chelerytrine, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chelerytrine/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    chelerytrine - by Bioz Stars, 2021-09
    92/100 stars
      Buy from Supplier

    94
    Alomone Labs anti trpc6 antibody
    Qualitative Western blots reveal the expression of canonical transient receptor proteins 3 and 7 (TRPC3 and TRPC7) in the VRG A) ʃVRG activity recorded from the whole medullary slice, in control conditions (ACSF). B) ʃVRG activity recorded from the VRG-island after its isolation from the rest of the medullary slice, in control conditions (ACSF). C) NK1 receptors (10μg total protein/ lanes 1-3); and, D) TRPC1 channels are expressed in the cortex, but not in the cerebellum or VRG-island preparations, 15μg total protein/ lanes). TRPC3 channels are expressed in all the VRG-islands tested (15μg total protein/ lanes 1-3). TRPC4 channels are expressed in the cortex and cerebellum but not the VRG-islands, (15μg total protein/ lanes), whereas TRPC5 channels (15μg total protein/ lanes) and <t>TRPC6</t> channels preparations, 15μg total protein/ lanes) are expressed in the cortex and, but not in the cerebellum or VRG-islands. TRPC7 channels are expressed in the cerebellum and VRG-islands (15μg total protein/ lanes). E ) TRPM4 channels were detected in the cortex (10μg total protein/ lanes 1 and 2) and cerebellum (10μg total protein/ lanes 1 and 2) but does not appear to be expressed in the VRG-Island (VRG-Island preparations; 10μg total protein/ lanes 1 and 3; 50μg total protein/lanes 2 and 4).
    Anti Trpc6 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti trpc6 antibody/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti trpc6 antibody - by Bioz Stars, 2021-09
    94/100 stars
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    Image Search Results


    Ca V γ4 is downregulated by silencing of the transcription factor MafA. a Rank of Pearson correlation coefficient ( R ) (tested by t -test) calculated by mRNA expression (Microarray, human islets) between Ca V γ4 ( CACNG4 ) and the transcription factors known for pancreas development. n = 128 donors. b Ca V γ4 mRNA expression in Pdx1, NeuroD1, MafA, Isl1, or Tcf7l2 silenced INS-1 cells. n = 3, ** p = 0.001 (NeuroD1), *** p

    Journal: Communications Biology

    Article Title: The calcium channel subunit gamma-4 is regulated by MafA and necessary for pancreatic beta-cell specification

    doi: 10.1038/s42003-019-0351-4

    Figure Lengend Snippet: Ca V γ4 is downregulated by silencing of the transcription factor MafA. a Rank of Pearson correlation coefficient ( R ) (tested by t -test) calculated by mRNA expression (Microarray, human islets) between Ca V γ4 ( CACNG4 ) and the transcription factors known for pancreas development. n = 128 donors. b Ca V γ4 mRNA expression in Pdx1, NeuroD1, MafA, Isl1, or Tcf7l2 silenced INS-1 cells. n = 3, ** p = 0.001 (NeuroD1), *** p

    Article Snippet: Afterwards, the membrane was incubated overnight at 4 °C with anti-CaV γ4 (1:400; Alomone labs), CaV 1.2 (1:500; Sigma), CaV 1.3 (1:400; Abcam), CaV α2 δ1 (1:500; Abcam), CaVβ1 (1:500; Abcam), Aldh1a3 (1:1000; Abcam), Cleaved Caspase-3 (1:1000; Cell Signaling), P21 (1:1000; Abcam) antibodies followed by incubation with anti-rabbit IgG (1:2000; Cell Signaling) or goat anti-mouse IgG (1:2000; Dako) at least 1 h at room temperature.

    Techniques: Expressing, Microarray

    Schematic of regulation cascade from MafA through Ca V γ4 to insulin secretion in beta cell. Compared with healthy beta cell, dedifferentiated beta cell caused by T2D or glucotoxicity results in reduced MafA expression, which leads to the downregulation of its direct downstream target Ca V γ4. Decreased Ca V γ4 expression then diminishes L-type Ca V channels expression with consequent preventing of Ca 2+ influx and in turn blunting of GSIS

    Journal: Communications Biology

    Article Title: The calcium channel subunit gamma-4 is regulated by MafA and necessary for pancreatic beta-cell specification

    doi: 10.1038/s42003-019-0351-4

    Figure Lengend Snippet: Schematic of regulation cascade from MafA through Ca V γ4 to insulin secretion in beta cell. Compared with healthy beta cell, dedifferentiated beta cell caused by T2D or glucotoxicity results in reduced MafA expression, which leads to the downregulation of its direct downstream target Ca V γ4. Decreased Ca V γ4 expression then diminishes L-type Ca V channels expression with consequent preventing of Ca 2+ influx and in turn blunting of GSIS

    Article Snippet: Afterwards, the membrane was incubated overnight at 4 °C with anti-CaV γ4 (1:400; Alomone labs), CaV 1.2 (1:500; Sigma), CaV 1.3 (1:400; Abcam), CaV α2 δ1 (1:500; Abcam), CaVβ1 (1:500; Abcam), Aldh1a3 (1:1000; Abcam), Cleaved Caspase-3 (1:1000; Cell Signaling), P21 (1:1000; Abcam) antibodies followed by incubation with anti-rabbit IgG (1:2000; Cell Signaling) or goat anti-mouse IgG (1:2000; Dako) at least 1 h at room temperature.

    Techniques: Expressing

    Decreased Ca V γ4 expression in beta cells in diabetes and in response to glucotoxicity. a Ca V γ4 ( CACNG4 ), Ca V γ5 ( CACNG5 ), and Ca V γ8 ( CACNG8 ) mRNA expression in human islets from donors with HbA1c

    Journal: Communications Biology

    Article Title: The calcium channel subunit gamma-4 is regulated by MafA and necessary for pancreatic beta-cell specification

    doi: 10.1038/s42003-019-0351-4

    Figure Lengend Snippet: Decreased Ca V γ4 expression in beta cells in diabetes and in response to glucotoxicity. a Ca V γ4 ( CACNG4 ), Ca V γ5 ( CACNG5 ), and Ca V γ8 ( CACNG8 ) mRNA expression in human islets from donors with HbA1c

    Article Snippet: Afterwards, the membrane was incubated overnight at 4 °C with anti-CaV γ4 (1:400; Alomone labs), CaV 1.2 (1:500; Sigma), CaV 1.3 (1:400; Abcam), CaV α2 δ1 (1:500; Abcam), CaVβ1 (1:500; Abcam), Aldh1a3 (1:1000; Abcam), Cleaved Caspase-3 (1:1000; Cell Signaling), P21 (1:1000; Abcam) antibodies followed by incubation with anti-rabbit IgG (1:2000; Cell Signaling) or goat anti-mouse IgG (1:2000; Dako) at least 1 h at room temperature.

    Techniques: Expressing

    Impact of Ca V γ4 on beta-cell function. a Glucose-stimulated insulin secretion (GSIS) in Ca V γ4-silenced Wistar rat islets. n = 4, ** p = 0.006. b As in a but in Ca V γ4-overexpressed GK rat islets. n = 6, * p = 0.028. c As in a but in Ca V γ4-silenced non-diabetic (ND) human islets. n = 5 donors, * p = 0.044. d As in a but in Ca V γ4-overexpressed T2D human islets. n = 1 donor ( p = 0.008 in 16.7mMG group by six technical repeats). e Reduced depolarization-evoked (V) exocytosis in Ca V γ4-silenced Wistar rat beta cells measured as an increase in membrane capacitance (Δ C ). f A summary of data in e presented as Δ C evoked by all 10 pulses of the train (Sum), the two first pulses (Phase 1) or the latter eight pulses (Phase 2). n = 15 control and 14 Ca V γ4-silencing cells, ** p = 0.009 (Sum), * p = 0.013 (Phase 1) and * p = 0.028 (Phase 2). g As in e but rescued exocytosis in Ca V γ4-overexpressed GK rat beta cells. h Summary of data in g . n = 15 control and 16 Ca V γ4-overexpressing cells, * p = 0.042 (Sum), p = 0.07 (Phase 1), and p = 0.058 (Phase 2). Data are presented as Mean ± SEM and were analyzed with two-tailed paired ( a – c ) or unpaired ( f , h ) Student’s t- test. OE overexpression, KD knockdown

    Journal: Communications Biology

    Article Title: The calcium channel subunit gamma-4 is regulated by MafA and necessary for pancreatic beta-cell specification

    doi: 10.1038/s42003-019-0351-4

    Figure Lengend Snippet: Impact of Ca V γ4 on beta-cell function. a Glucose-stimulated insulin secretion (GSIS) in Ca V γ4-silenced Wistar rat islets. n = 4, ** p = 0.006. b As in a but in Ca V γ4-overexpressed GK rat islets. n = 6, * p = 0.028. c As in a but in Ca V γ4-silenced non-diabetic (ND) human islets. n = 5 donors, * p = 0.044. d As in a but in Ca V γ4-overexpressed T2D human islets. n = 1 donor ( p = 0.008 in 16.7mMG group by six technical repeats). e Reduced depolarization-evoked (V) exocytosis in Ca V γ4-silenced Wistar rat beta cells measured as an increase in membrane capacitance (Δ C ). f A summary of data in e presented as Δ C evoked by all 10 pulses of the train (Sum), the two first pulses (Phase 1) or the latter eight pulses (Phase 2). n = 15 control and 14 Ca V γ4-silencing cells, ** p = 0.009 (Sum), * p = 0.013 (Phase 1) and * p = 0.028 (Phase 2). g As in e but rescued exocytosis in Ca V γ4-overexpressed GK rat beta cells. h Summary of data in g . n = 15 control and 16 Ca V γ4-overexpressing cells, * p = 0.042 (Sum), p = 0.07 (Phase 1), and p = 0.058 (Phase 2). Data are presented as Mean ± SEM and were analyzed with two-tailed paired ( a – c ) or unpaired ( f , h ) Student’s t- test. OE overexpression, KD knockdown

    Article Snippet: Afterwards, the membrane was incubated overnight at 4 °C with anti-CaV γ4 (1:400; Alomone labs), CaV 1.2 (1:500; Sigma), CaV 1.3 (1:400; Abcam), CaV α2 δ1 (1:500; Abcam), CaVβ1 (1:500; Abcam), Aldh1a3 (1:1000; Abcam), Cleaved Caspase-3 (1:1000; Cell Signaling), P21 (1:1000; Abcam) antibodies followed by incubation with anti-rabbit IgG (1:2000; Cell Signaling) or goat anti-mouse IgG (1:2000; Dako) at least 1 h at room temperature.

    Techniques: Cell Function Assay, Two Tailed Test, Over Expression

    Downregulation of L-type Ca V channels in Ca V γ4-silenced beta cells. a Correlation of mRNA expression (Microarray) between Ca V γ4 ( CACNG4 ) and L-type Ca V channels in human islets. n = 128 donors. Pearson correlation coefficient ( R ) was tested ( t -test) and labeled alongside with p values. b Ca V 1.2 ( CACNA1C ) and Ca V 1.3 ( CACNA1D ) mRNA expression (qPCR) in Ca V γ4-silenced human islets. n = 3 donors, * p = 0.032 (Ca V 1.2), * p = 0.011 (Ca V 1.3). c As in b but in Ca V γ4-overexpressed human islets. n = 3 donors, ** p = 0.005 (Ca V 1.2), * p = 0.039 (Ca V 1.3). d Ca V channel immunoblotting and means of expression in Ca V γ4-silenced INS-1 cells. n = 4, *** p

    Journal: Communications Biology

    Article Title: The calcium channel subunit gamma-4 is regulated by MafA and necessary for pancreatic beta-cell specification

    doi: 10.1038/s42003-019-0351-4

    Figure Lengend Snippet: Downregulation of L-type Ca V channels in Ca V γ4-silenced beta cells. a Correlation of mRNA expression (Microarray) between Ca V γ4 ( CACNG4 ) and L-type Ca V channels in human islets. n = 128 donors. Pearson correlation coefficient ( R ) was tested ( t -test) and labeled alongside with p values. b Ca V 1.2 ( CACNA1C ) and Ca V 1.3 ( CACNA1D ) mRNA expression (qPCR) in Ca V γ4-silenced human islets. n = 3 donors, * p = 0.032 (Ca V 1.2), * p = 0.011 (Ca V 1.3). c As in b but in Ca V γ4-overexpressed human islets. n = 3 donors, ** p = 0.005 (Ca V 1.2), * p = 0.039 (Ca V 1.3). d Ca V channel immunoblotting and means of expression in Ca V γ4-silenced INS-1 cells. n = 4, *** p

    Article Snippet: Afterwards, the membrane was incubated overnight at 4 °C with anti-CaV γ4 (1:400; Alomone labs), CaV 1.2 (1:500; Sigma), CaV 1.3 (1:400; Abcam), CaV α2 δ1 (1:500; Abcam), CaVβ1 (1:500; Abcam), Aldh1a3 (1:1000; Abcam), Cleaved Caspase-3 (1:1000; Cell Signaling), P21 (1:1000; Abcam) antibodies followed by incubation with anti-rabbit IgG (1:2000; Cell Signaling) or goat anti-mouse IgG (1:2000; Dako) at least 1 h at room temperature.

    Techniques: Expressing, Microarray, Labeling, Real-time Polymerase Chain Reaction

    Boost of Ca 2+ influx in Ca V γ4-overexpressed beta cells. a Ca 2+ currents records in Ca V γ4-overexpressed non-diabetic (ND) human beta cells. n = 12 control and 13 Ca V γ4-overexpressing cells (4 donors), * p = 0.048. b As in a but in T2D human beta cells. n = 7 control and 8 overexpressing cells (1 T2D donor), * p = 0.032 (−10 mV), **0.004 (0 mV), *0.028 (10 mV). c As in a but in Ca V γ4-silenced Wistar rat beta cells. n = 15 control and 14 silencing cells, * p = 0.040 (0 mV), *0.034 (10 mV). d As in a but in Ca V γ4-overexpressed GK rat beta cells. n = 15 control and 14 overexpressing cells, * p = 0.047. e As in a but in Ca V γ4-overexpressed Wistar rat beta cells. n = 16 cells each, ** p = 0.003 (0 mV), **0.006 (10 mV). f Ca 2+ imaging in control, Ca V γ4, or Ca V γ5 silenced INS-1 cells. g Comparisons of [Ca 2+ ] i peak intensity (F i /F 0 ) in f , *** p

    Journal: Communications Biology

    Article Title: The calcium channel subunit gamma-4 is regulated by MafA and necessary for pancreatic beta-cell specification

    doi: 10.1038/s42003-019-0351-4

    Figure Lengend Snippet: Boost of Ca 2+ influx in Ca V γ4-overexpressed beta cells. a Ca 2+ currents records in Ca V γ4-overexpressed non-diabetic (ND) human beta cells. n = 12 control and 13 Ca V γ4-overexpressing cells (4 donors), * p = 0.048. b As in a but in T2D human beta cells. n = 7 control and 8 overexpressing cells (1 T2D donor), * p = 0.032 (−10 mV), **0.004 (0 mV), *0.028 (10 mV). c As in a but in Ca V γ4-silenced Wistar rat beta cells. n = 15 control and 14 silencing cells, * p = 0.040 (0 mV), *0.034 (10 mV). d As in a but in Ca V γ4-overexpressed GK rat beta cells. n = 15 control and 14 overexpressing cells, * p = 0.047. e As in a but in Ca V γ4-overexpressed Wistar rat beta cells. n = 16 cells each, ** p = 0.003 (0 mV), **0.006 (10 mV). f Ca 2+ imaging in control, Ca V γ4, or Ca V γ5 silenced INS-1 cells. g Comparisons of [Ca 2+ ] i peak intensity (F i /F 0 ) in f , *** p

    Article Snippet: Afterwards, the membrane was incubated overnight at 4 °C with anti-CaV γ4 (1:400; Alomone labs), CaV 1.2 (1:500; Sigma), CaV 1.3 (1:400; Abcam), CaV α2 δ1 (1:500; Abcam), CaVβ1 (1:500; Abcam), Aldh1a3 (1:1000; Abcam), Cleaved Caspase-3 (1:1000; Cell Signaling), P21 (1:1000; Abcam) antibodies followed by incubation with anti-rabbit IgG (1:2000; Cell Signaling) or goat anti-mouse IgG (1:2000; Dako) at least 1 h at room temperature.

    Techniques: Imaging

    WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p

    Journal: The Turkish Journal of Gastroenterology

    Article Title: TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model

    doi: 10.5152/tjg.2018.17817

    Figure Lengend Snippet: WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p

    Article Snippet: Sections were incubated for 12 hours at 4°C with rabbit anti-TRPA1 (1:400, Alomone labs, ACC-037, Jerusalem, Israel) or mouse anti-SP (1:200, R & D SYSTEMS, MAB4375, USA).

    Techniques: Expressing, Western Blot, Immunohistochemistry

    Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)

    Journal: The Turkish Journal of Gastroenterology

    Article Title: TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model

    doi: 10.5152/tjg.2018.17817

    Figure Lengend Snippet: Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)

    Article Snippet: Sections were incubated for 12 hours at 4°C with rabbit anti-TRPA1 (1:400, Alomone labs, ACC-037, Jerusalem, Israel) or mouse anti-SP (1:200, R & D SYSTEMS, MAB4375, USA).

    Techniques: Expressing, Western Blot, Immunohistochemistry

    PKC activity modulation in axonal loss. ( A ) shows the percentage of singly-, doubly-, and triply- (or more) innervated NMJs in the control mice (PBS) and after four applications of one of the following substances: the PKC paninhibitors Chelerytrine (CHE) and CaC, and the PKC panstimulators (BRY, at 1 and 10 nM) and PMA. CHE and CaC action results in the persistence of many polyinnervated synapses. Accordingly, both PKC stimulators PMA and BRY increase the number of monoinnervated junctions and clearly decrease the percentage of doubly-innervated junctions in the case of BRY. ( B ) shows the percentage of singly-, doubly-, and triply (or more) innervated NMJs after exposure to the cPKCβI and nPKCε isoform selective inhibitors βIV 5–3 and εV 1–2 , and the cPKCβI and nPKCε selective activators dPPA and FR 236,924 respectively. The selective inhibitors similarly increase the doubly- and triply-innervated synapses with the corresponding reduction in the monoinnervated junctions. The activators considerably accelerate nerve terminal elimination. Data were presented as percentages of NMJ ± SD (for each treatment and PBS control: n pups = 6–9; n = 11–18 LALs; n NMJ: PBS: 2538; Bry 1 nM: 1232 Bry 10 nM:1384; PMA:1247; CaC: 1573; Che:1499; βIV 5–3 : 1275; dPPA: 1121; εV 1–2 :1663 and FR 236924: 1367). Fisher’s test: * p

    Journal: Cells

    Article Title: Opposed Actions of PKA Isozymes (RI and RII) and PKC Isoforms (cPKCβI and nPKCε) in Neuromuscular Developmental Synapse Elimination

    doi: 10.3390/cells8111304

    Figure Lengend Snippet: PKC activity modulation in axonal loss. ( A ) shows the percentage of singly-, doubly-, and triply- (or more) innervated NMJs in the control mice (PBS) and after four applications of one of the following substances: the PKC paninhibitors Chelerytrine (CHE) and CaC, and the PKC panstimulators (BRY, at 1 and 10 nM) and PMA. CHE and CaC action results in the persistence of many polyinnervated synapses. Accordingly, both PKC stimulators PMA and BRY increase the number of monoinnervated junctions and clearly decrease the percentage of doubly-innervated junctions in the case of BRY. ( B ) shows the percentage of singly-, doubly-, and triply (or more) innervated NMJs after exposure to the cPKCβI and nPKCε isoform selective inhibitors βIV 5–3 and εV 1–2 , and the cPKCβI and nPKCε selective activators dPPA and FR 236,924 respectively. The selective inhibitors similarly increase the doubly- and triply-innervated synapses with the corresponding reduction in the monoinnervated junctions. The activators considerably accelerate nerve terminal elimination. Data were presented as percentages of NMJ ± SD (for each treatment and PBS control: n pups = 6–9; n = 11–18 LALs; n NMJ: PBS: 2538; Bry 1 nM: 1232 Bry 10 nM:1384; PMA:1247; CaC: 1573; Che:1499; βIV 5–3 : 1275; dPPA: 1121; εV 1–2 :1663 and FR 236924: 1367). Fisher’s test: * p

    Article Snippet: Selective PKC Substances Antagonists : The stock solutions were Chelerytrine (CHE, C-400, Alomone, Jerusalem, Israel) 10 mM; Calfostein C (CaC, C6303, Sigma-Aldrich) 2.5 mM; Peptide βIV 5–3 (βIV 5–3 Mochly Rosen, Standford University) 10 mM; Peptide εV 1–2 , (εV 1–2, 539522.

    Techniques: Activity Assay, Mouse Assay

    Qualitative Western blots reveal the expression of canonical transient receptor proteins 3 and 7 (TRPC3 and TRPC7) in the VRG A) ʃVRG activity recorded from the whole medullary slice, in control conditions (ACSF). B) ʃVRG activity recorded from the VRG-island after its isolation from the rest of the medullary slice, in control conditions (ACSF). C) NK1 receptors (10μg total protein/ lanes 1-3); and, D) TRPC1 channels are expressed in the cortex, but not in the cerebellum or VRG-island preparations, 15μg total protein/ lanes). TRPC3 channels are expressed in all the VRG-islands tested (15μg total protein/ lanes 1-3). TRPC4 channels are expressed in the cortex and cerebellum but not the VRG-islands, (15μg total protein/ lanes), whereas TRPC5 channels (15μg total protein/ lanes) and TRPC6 channels preparations, 15μg total protein/ lanes) are expressed in the cortex and, but not in the cerebellum or VRG-islands. TRPC7 channels are expressed in the cerebellum and VRG-islands (15μg total protein/ lanes). E ) TRPM4 channels were detected in the cortex (10μg total protein/ lanes 1 and 2) and cerebellum (10μg total protein/ lanes 1 and 2) but does not appear to be expressed in the VRG-Island (VRG-Island preparations; 10μg total protein/ lanes 1 and 3; 50μg total protein/lanes 2 and 4).

    Journal: The European journal of neuroscience

    Article Title: Substance P modulation of TRPC3/7 channels improves respiratory rhythm regularity and ICAN-dependent pacemaker activity

    doi: 10.1111/j.1460-9568.2010.07156.x

    Figure Lengend Snippet: Qualitative Western blots reveal the expression of canonical transient receptor proteins 3 and 7 (TRPC3 and TRPC7) in the VRG A) ʃVRG activity recorded from the whole medullary slice, in control conditions (ACSF). B) ʃVRG activity recorded from the VRG-island after its isolation from the rest of the medullary slice, in control conditions (ACSF). C) NK1 receptors (10μg total protein/ lanes 1-3); and, D) TRPC1 channels are expressed in the cortex, but not in the cerebellum or VRG-island preparations, 15μg total protein/ lanes). TRPC3 channels are expressed in all the VRG-islands tested (15μg total protein/ lanes 1-3). TRPC4 channels are expressed in the cortex and cerebellum but not the VRG-islands, (15μg total protein/ lanes), whereas TRPC5 channels (15μg total protein/ lanes) and TRPC6 channels preparations, 15μg total protein/ lanes) are expressed in the cortex and, but not in the cerebellum or VRG-islands. TRPC7 channels are expressed in the cerebellum and VRG-islands (15μg total protein/ lanes). E ) TRPM4 channels were detected in the cortex (10μg total protein/ lanes 1 and 2) and cerebellum (10μg total protein/ lanes 1 and 2) but does not appear to be expressed in the VRG-Island (VRG-Island preparations; 10μg total protein/ lanes 1 and 3; 50μg total protein/lanes 2 and 4).

    Article Snippet: The membranes were blocked overnight at +4°C with 5% non-fat dried milk (Bio-Rad Labs, USA) and 1% albumin from bovine serum (Sigma, USA) in Tris Buffered Saline, pH 7.5, containing 0.1% Tween-20 (TBS-T) and immuno-blotted for 2h at room temperature with either anti-NK1 antibody (1:10000) (Invitrogen, USA) or anti-TRPC1 antibody (1:500) (Alomone labs, Israel), or anti-TRPC3 antibody (1:500) (Alomone labs, Israel) or anti-TRPC4 antibody (1:100) (Alomone labs, Israel), or anti-TRPC5 antibody (1:300) (Santa Cruz, USA), or anti-TRPC6 antibody (1:400) (Alomone labs, Israel), or anti-TRPC7 antibody (1:300) (Millipore, USA), anti-TRPM4 antibody (1:1000) (Abcam, USA) in 2% NFDM in TBS-T, anti-GAPDH (1:300) (Abcam, USA) used as a loading control.

    Techniques: Western Blot, Expressing, Activity Assay, Isolation