Journal: STAR Protocols
Article Title: Protocol for chromatin accessibility profiling of human endothelial cells cultured under fluid shear stress using ATAC-seq
doi: 10.1016/j.xpro.2024.102859
Figure Lengend Snippet: Endothelial cell morphology (A‒C) Endothelial cells under a phase contrast microscope. Scale bars: 100 μm (A) Before applying FSS, the cell monolayer should be grown to full confluency. (B) Since growth rates of different primary ECs varies, cells can be subconfluent on the day of the intended experiment start. In that case it is advised to delay the experiment one or two days until confluency is reached. (C) If ECs exhibit an abnormal phenotype, i.e., no (A) cobble-stone like EC-monolayer, on the day of the experiment, it is advised to restart the experiment.
Article Snippet: Seed 5 × 105 primary Human Umbilical Artery Endothelial Cells (HUAECs; PromoCell GmbH, Germany) in 12 mL full medium to a T75 flask.
Techniques: Microscopy