Journal: The Journal of Biological Chemistry
Article Title: Melatonin downregulates TRPC6, impairing store-operated calcium entry in triple-negative breast cancer cells
Figure Lengend Snippet: Expression of exogenous TRPC6 reverses the antiproliferative effect of melatonin in MDA-MB-231 cells. MDA-MB-231 cells were treated for 48, 72, and 168 h with melatonin (10–1000 nM) or the vehicle (control). Forty-eight hours before the end of the treatment period, cells were transfected with TRPC6-YFP ( B , E , and H ) or TRPC6dn-YFP ( C , F , and I ) expression plasmid or empty vector ( A , D and G ). Cell proliferation was assessed at time = 0, 24, 48, and 72 h using the BrdU cell proliferation assay kit, as described in Experimental procedures . Scatter plots represent cell proliferation 0, 24, 48, and 72 h after the onset of the experiment, presented as BrdU uptake rate (n = 6). Analysis of statistical significance was performed using two-way ANOVA (for A , D , G : 0 h, F values were 0.14, 0.14, and 1.35 and p values were 0.93, 0.86, and 0.24 for concentration, time, and the interaction respectively; for A , D , G : 24 h, F values were 593.8, 1570, and 199.6 and p values were
Article Snippet: Rabbit polyclonal anti-TRPC6 antibody (catalog number: ACC-120, epitope corresponding to amino acid residues 573–586) was from Alomone.
Techniques: Expressing, Multiple Displacement Amplification, Transfection, Plasmid Preparation, BrdU Cell Proliferation Assay, Concentration Assay