cyppa  (Alomone Labs)

Journal: Cancers

Article Title: Orai1 Boosts SK3 Channel Activation

doi: 10.3390/cancers13246357

Figure Lengend Snippet: SK3–Orai1 interplay in LNCaP cells: Inset table and chemical structures represent the used agonist and antagonist of SK and Orai1 channels. ( A ) Cell viability of LNCaP cells after 24, 48, 72, and 96 h upon the treatment with SK3 channel agonist 10 μM Cyppa, 5 μM NS309, and antagonist 30 μM NS8593, 10 mM 4-AP detected via MTS assay; ( B ) Block diagram represents the cell proliferation of LNCaP cells after 96 h upon conditions described in ( A ). The Mann–Whitney test was used for statistical comparison considering differences statistically significant at p

Article Snippet: Inhibitors (NS8593 hydrochloride Cat #: N-195, 4-AP Cat #: A-115) and activators (NS-309 Cat #: N-180, 1-EBIO Cat #: E-150, Cyppa Cat #: C-110) were purchased from Alomone Labs (Jerusalem, Israel).

Techniques: MTS Assay, Blocking Assay, MANN-WHITNEY

Journal: Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology

Article Title: Ethanol’s Effects on Transient Receptor Potential Channel Expression in Brain Microvascular Endothelial Cells

doi: 10.1007/s11481-018-9796-3

Figure Lengend Snippet: Endothelial cell barrier permeability following treatment with a) different concentrations of EtOH or b) the TRPM7 antagonists, NS8593, CyPPA, or SKA-31. * p

Article Snippet: For Trpm 7 inhibition, three different Trpm 7 Channel Blockers, NS 8593, CyPPA and SKA-31 (Alomone Labs, Jerusalem, Israel) were added to the inserts at 50 μM, together with a tracer Sodium Fluorescein (NaF) at 10 μg/mL, and incubated in a humidified cell culture incubator at 37 °C with 5% CO2 for 24 h. One hundred microliter samples from both the upper insert and the lower chamber were transferred to a clear 96-well plate.

Techniques: Permeability