kcnq1  (Alomone Labs)


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    Structured Review

    Alomone Labs kcnq1
    Increased intracellular cAMP reduces <t>hERG-KCNQ1</t> interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP
    Kcnq1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 85/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kcnq1/product/Alomone Labs
    Average 85 stars, based on 40 article reviews
    Price from $9.99 to $1999.99
    kcnq1 - by Bioz Stars, 2022-09
    85/100 stars

    Images

    1) Product Images from "Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP"

    Article Title: Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    doi: 10.1152/ajpheart.00385.2012

    Increased intracellular cAMP reduces hERG-KCNQ1 interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP
    Figure Legend Snippet: Increased intracellular cAMP reduces hERG-KCNQ1 interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP

    Techniques Used: Construct, Labeling

    hERG-mCFP + KCNQ1-mYFP expression and interaction in cultured neonatal rabbit cardiomyocytes (NRbCM). A : 4–5 days postisolation, cultures have a myocyte purity of 80%, as evidenced by double immunofluorescent labeling for vimentin (fibroblasts,
    Figure Legend Snippet: hERG-mCFP + KCNQ1-mYFP expression and interaction in cultured neonatal rabbit cardiomyocytes (NRbCM). A : 4–5 days postisolation, cultures have a myocyte purity of 80%, as evidenced by double immunofluorescent labeling for vimentin (fibroblasts,

    Techniques Used: Expressing, Cell Culture, Labeling

    Co-expression of paired KCNQ1 and hERG constructs and raw apFRET data demonstrate differences based on label placement. A–D : FRET pairs are indicated, representing the four possible combinations of fluorophore placement. Top : representative images
    Figure Legend Snippet: Co-expression of paired KCNQ1 and hERG constructs and raw apFRET data demonstrate differences based on label placement. A–D : FRET pairs are indicated, representing the four possible combinations of fluorophore placement. Top : representative images

    Techniques Used: Expressing, Construct

    apFRET demonstrates significant interactions between KCNQ1 and hERG fusion constructs. A : average FRET efficiencies for overexpression and positive controls as well as the four pairs varying KCNQ1 and hERG fluorophore placement. C and Y indicate mCFP
    Figure Legend Snippet: apFRET demonstrates significant interactions between KCNQ1 and hERG fusion constructs. A : average FRET efficiencies for overexpression and positive controls as well as the four pairs varying KCNQ1 and hERG fluorophore placement. C and Y indicate mCFP

    Techniques Used: Construct, Over Expression

    2) Product Images from "Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP"

    Article Title: Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    doi: 10.1152/ajpheart.00385.2012

    Increased intracellular cAMP reduces hERG-KCNQ1 interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP
    Figure Legend Snippet: Increased intracellular cAMP reduces hERG-KCNQ1 interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP

    Techniques Used: Construct, Labeling

    hERG-mCFP + KCNQ1-mYFP expression and interaction in cultured neonatal rabbit cardiomyocytes (NRbCM). A : 4–5 days postisolation, cultures have a myocyte purity of 80%, as evidenced by double immunofluorescent labeling for vimentin (fibroblasts,
    Figure Legend Snippet: hERG-mCFP + KCNQ1-mYFP expression and interaction in cultured neonatal rabbit cardiomyocytes (NRbCM). A : 4–5 days postisolation, cultures have a myocyte purity of 80%, as evidenced by double immunofluorescent labeling for vimentin (fibroblasts,

    Techniques Used: Expressing, Cell Culture, Labeling

    Co-expression of paired KCNQ1 and hERG constructs and raw apFRET data demonstrate differences based on label placement. A–D : FRET pairs are indicated, representing the four possible combinations of fluorophore placement. Top : representative images
    Figure Legend Snippet: Co-expression of paired KCNQ1 and hERG constructs and raw apFRET data demonstrate differences based on label placement. A–D : FRET pairs are indicated, representing the four possible combinations of fluorophore placement. Top : representative images

    Techniques Used: Expressing, Construct

    apFRET demonstrates significant interactions between KCNQ1 and hERG fusion constructs. A : average FRET efficiencies for overexpression and positive controls as well as the four pairs varying KCNQ1 and hERG fluorophore placement. C and Y indicate mCFP
    Figure Legend Snippet: apFRET demonstrates significant interactions between KCNQ1 and hERG fusion constructs. A : average FRET efficiencies for overexpression and positive controls as well as the four pairs varying KCNQ1 and hERG fluorophore placement. C and Y indicate mCFP

    Techniques Used: Construct, Over Expression

    3) Product Images from "Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP"

    Article Title: Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    doi: 10.1152/ajpheart.00385.2012

    Increased intracellular cAMP reduces hERG-KCNQ1 interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP
    Figure Legend Snippet: Increased intracellular cAMP reduces hERG-KCNQ1 interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP

    Techniques Used: Construct, Labeling

    hERG-mCFP + KCNQ1-mYFP expression and interaction in cultured neonatal rabbit cardiomyocytes (NRbCM). A : 4–5 days postisolation, cultures have a myocyte purity of 80%, as evidenced by double immunofluorescent labeling for vimentin (fibroblasts,
    Figure Legend Snippet: hERG-mCFP + KCNQ1-mYFP expression and interaction in cultured neonatal rabbit cardiomyocytes (NRbCM). A : 4–5 days postisolation, cultures have a myocyte purity of 80%, as evidenced by double immunofluorescent labeling for vimentin (fibroblasts,

    Techniques Used: Expressing, Cell Culture, Labeling

    Co-expression of paired KCNQ1 and hERG constructs and raw apFRET data demonstrate differences based on label placement. A–D : FRET pairs are indicated, representing the four possible combinations of fluorophore placement. Top : representative images
    Figure Legend Snippet: Co-expression of paired KCNQ1 and hERG constructs and raw apFRET data demonstrate differences based on label placement. A–D : FRET pairs are indicated, representing the four possible combinations of fluorophore placement. Top : representative images

    Techniques Used: Expressing, Construct

    apFRET demonstrates significant interactions between KCNQ1 and hERG fusion constructs. A : average FRET efficiencies for overexpression and positive controls as well as the four pairs varying KCNQ1 and hERG fluorophore placement. C and Y indicate mCFP
    Figure Legend Snippet: apFRET demonstrates significant interactions between KCNQ1 and hERG fusion constructs. A : average FRET efficiencies for overexpression and positive controls as well as the four pairs varying KCNQ1 and hERG fluorophore placement. C and Y indicate mCFP

    Techniques Used: Construct, Over Expression

    4) Product Images from "Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP"

    Article Title: Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    doi: 10.1152/ajpheart.00385.2012

    Increased intracellular cAMP reduces hERG-KCNQ1 interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP
    Figure Legend Snippet: Increased intracellular cAMP reduces hERG-KCNQ1 interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP

    Techniques Used: Construct, Labeling

    hERG-mCFP + KCNQ1-mYFP expression and interaction in cultured neonatal rabbit cardiomyocytes (NRbCM). A : 4–5 days postisolation, cultures have a myocyte purity of 80%, as evidenced by double immunofluorescent labeling for vimentin (fibroblasts,
    Figure Legend Snippet: hERG-mCFP + KCNQ1-mYFP expression and interaction in cultured neonatal rabbit cardiomyocytes (NRbCM). A : 4–5 days postisolation, cultures have a myocyte purity of 80%, as evidenced by double immunofluorescent labeling for vimentin (fibroblasts,

    Techniques Used: Expressing, Cell Culture, Labeling

    Co-expression of paired KCNQ1 and hERG constructs and raw apFRET data demonstrate differences based on label placement. A–D : FRET pairs are indicated, representing the four possible combinations of fluorophore placement. Top : representative images
    Figure Legend Snippet: Co-expression of paired KCNQ1 and hERG constructs and raw apFRET data demonstrate differences based on label placement. A–D : FRET pairs are indicated, representing the four possible combinations of fluorophore placement. Top : representative images

    Techniques Used: Expressing, Construct

    apFRET demonstrates significant interactions between KCNQ1 and hERG fusion constructs. A : average FRET efficiencies for overexpression and positive controls as well as the four pairs varying KCNQ1 and hERG fluorophore placement. C and Y indicate mCFP
    Figure Legend Snippet: apFRET demonstrates significant interactions between KCNQ1 and hERG fusion constructs. A : average FRET efficiencies for overexpression and positive controls as well as the four pairs varying KCNQ1 and hERG fluorophore placement. C and Y indicate mCFP

    Techniques Used: Construct, Over Expression

    5) Product Images from "Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP"

    Article Title: Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    doi: 10.1152/ajpheart.00385.2012

    Increased intracellular cAMP reduces hERG-KCNQ1 interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP
    Figure Legend Snippet: Increased intracellular cAMP reduces hERG-KCNQ1 interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP

    Techniques Used: Construct, Labeling

    hERG-mCFP + KCNQ1-mYFP expression and interaction in cultured neonatal rabbit cardiomyocytes (NRbCM). A : 4–5 days postisolation, cultures have a myocyte purity of 80%, as evidenced by double immunofluorescent labeling for vimentin (fibroblasts,
    Figure Legend Snippet: hERG-mCFP + KCNQ1-mYFP expression and interaction in cultured neonatal rabbit cardiomyocytes (NRbCM). A : 4–5 days postisolation, cultures have a myocyte purity of 80%, as evidenced by double immunofluorescent labeling for vimentin (fibroblasts,

    Techniques Used: Expressing, Cell Culture, Labeling

    Co-expression of paired KCNQ1 and hERG constructs and raw apFRET data demonstrate differences based on label placement. A–D : FRET pairs are indicated, representing the four possible combinations of fluorophore placement. Top : representative images
    Figure Legend Snippet: Co-expression of paired KCNQ1 and hERG constructs and raw apFRET data demonstrate differences based on label placement. A–D : FRET pairs are indicated, representing the four possible combinations of fluorophore placement. Top : representative images

    Techniques Used: Expressing, Construct

    apFRET demonstrates significant interactions between KCNQ1 and hERG fusion constructs. A : average FRET efficiencies for overexpression and positive controls as well as the four pairs varying KCNQ1 and hERG fluorophore placement. C and Y indicate mCFP
    Figure Legend Snippet: apFRET demonstrates significant interactions between KCNQ1 and hERG fusion constructs. A : average FRET efficiencies for overexpression and positive controls as well as the four pairs varying KCNQ1 and hERG fluorophore placement. C and Y indicate mCFP

    Techniques Used: Construct, Over Expression

    6) Product Images from "Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP"

    Article Title: Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    doi: 10.1152/ajpheart.00385.2012

    Increased intracellular cAMP reduces hERG-KCNQ1 interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP
    Figure Legend Snippet: Increased intracellular cAMP reduces hERG-KCNQ1 interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP

    Techniques Used: Construct, Labeling

    hERG-mCFP + KCNQ1-mYFP expression and interaction in cultured neonatal rabbit cardiomyocytes (NRbCM). A : 4–5 days postisolation, cultures have a myocyte purity of 80%, as evidenced by double immunofluorescent labeling for vimentin (fibroblasts,
    Figure Legend Snippet: hERG-mCFP + KCNQ1-mYFP expression and interaction in cultured neonatal rabbit cardiomyocytes (NRbCM). A : 4–5 days postisolation, cultures have a myocyte purity of 80%, as evidenced by double immunofluorescent labeling for vimentin (fibroblasts,

    Techniques Used: Expressing, Cell Culture, Labeling

    Co-expression of paired KCNQ1 and hERG constructs and raw apFRET data demonstrate differences based on label placement. A–D : FRET pairs are indicated, representing the four possible combinations of fluorophore placement. Top : representative images
    Figure Legend Snippet: Co-expression of paired KCNQ1 and hERG constructs and raw apFRET data demonstrate differences based on label placement. A–D : FRET pairs are indicated, representing the four possible combinations of fluorophore placement. Top : representative images

    Techniques Used: Expressing, Construct

    apFRET demonstrates significant interactions between KCNQ1 and hERG fusion constructs. A : average FRET efficiencies for overexpression and positive controls as well as the four pairs varying KCNQ1 and hERG fluorophore placement. C and Y indicate mCFP
    Figure Legend Snippet: apFRET demonstrates significant interactions between KCNQ1 and hERG fusion constructs. A : average FRET efficiencies for overexpression and positive controls as well as the four pairs varying KCNQ1 and hERG fluorophore placement. C and Y indicate mCFP

    Techniques Used: Construct, Over Expression

    7) Product Images from "Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP"

    Article Title: Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    doi: 10.1152/ajpheart.00385.2012

    Increased intracellular cAMP reduces hERG-KCNQ1 interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP
    Figure Legend Snippet: Increased intracellular cAMP reduces hERG-KCNQ1 interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP

    Techniques Used: Construct, Labeling

    hERG-mCFP + KCNQ1-mYFP expression and interaction in cultured neonatal rabbit cardiomyocytes (NRbCM). A : 4–5 days postisolation, cultures have a myocyte purity of 80%, as evidenced by double immunofluorescent labeling for vimentin (fibroblasts,
    Figure Legend Snippet: hERG-mCFP + KCNQ1-mYFP expression and interaction in cultured neonatal rabbit cardiomyocytes (NRbCM). A : 4–5 days postisolation, cultures have a myocyte purity of 80%, as evidenced by double immunofluorescent labeling for vimentin (fibroblasts,

    Techniques Used: Expressing, Cell Culture, Labeling

    Co-expression of paired KCNQ1 and hERG constructs and raw apFRET data demonstrate differences based on label placement. A–D : FRET pairs are indicated, representing the four possible combinations of fluorophore placement. Top : representative images
    Figure Legend Snippet: Co-expression of paired KCNQ1 and hERG constructs and raw apFRET data demonstrate differences based on label placement. A–D : FRET pairs are indicated, representing the four possible combinations of fluorophore placement. Top : representative images

    Techniques Used: Expressing, Construct

    apFRET demonstrates significant interactions between KCNQ1 and hERG fusion constructs. A : average FRET efficiencies for overexpression and positive controls as well as the four pairs varying KCNQ1 and hERG fluorophore placement. C and Y indicate mCFP
    Figure Legend Snippet: apFRET demonstrates significant interactions between KCNQ1 and hERG fusion constructs. A : average FRET efficiencies for overexpression and positive controls as well as the four pairs varying KCNQ1 and hERG fluorophore placement. C and Y indicate mCFP

    Techniques Used: Construct, Over Expression

    8) Product Images from "Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP"

    Article Title: Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    doi: 10.1152/ajpheart.00385.2012

    Increased intracellular cAMP reduces hERG-KCNQ1 interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP
    Figure Legend Snippet: Increased intracellular cAMP reduces hERG-KCNQ1 interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP

    Techniques Used: Construct, Labeling

    hERG-mCFP + KCNQ1-mYFP expression and interaction in cultured neonatal rabbit cardiomyocytes (NRbCM). A : 4–5 days postisolation, cultures have a myocyte purity of 80%, as evidenced by double immunofluorescent labeling for vimentin (fibroblasts,
    Figure Legend Snippet: hERG-mCFP + KCNQ1-mYFP expression and interaction in cultured neonatal rabbit cardiomyocytes (NRbCM). A : 4–5 days postisolation, cultures have a myocyte purity of 80%, as evidenced by double immunofluorescent labeling for vimentin (fibroblasts,

    Techniques Used: Expressing, Cell Culture, Labeling

    Co-expression of paired KCNQ1 and hERG constructs and raw apFRET data demonstrate differences based on label placement. A–D : FRET pairs are indicated, representing the four possible combinations of fluorophore placement. Top : representative images
    Figure Legend Snippet: Co-expression of paired KCNQ1 and hERG constructs and raw apFRET data demonstrate differences based on label placement. A–D : FRET pairs are indicated, representing the four possible combinations of fluorophore placement. Top : representative images

    Techniques Used: Expressing, Construct

    apFRET demonstrates significant interactions between KCNQ1 and hERG fusion constructs. A : average FRET efficiencies for overexpression and positive controls as well as the four pairs varying KCNQ1 and hERG fluorophore placement. C and Y indicate mCFP
    Figure Legend Snippet: apFRET demonstrates significant interactions between KCNQ1 and hERG fusion constructs. A : average FRET efficiencies for overexpression and positive controls as well as the four pairs varying KCNQ1 and hERG fluorophore placement. C and Y indicate mCFP

    Techniques Used: Construct, Over Expression

    9) Product Images from "Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP"

    Article Title: Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    doi: 10.1152/ajpheart.00385.2012

    Increased intracellular cAMP reduces hERG-KCNQ1 interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP
    Figure Legend Snippet: Increased intracellular cAMP reduces hERG-KCNQ1 interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP

    Techniques Used: Construct, Labeling

    hERG-mCFP + KCNQ1-mYFP expression and interaction in cultured neonatal rabbit cardiomyocytes (NRbCM). A : 4–5 days postisolation, cultures have a myocyte purity of 80%, as evidenced by double immunofluorescent labeling for vimentin (fibroblasts,
    Figure Legend Snippet: hERG-mCFP + KCNQ1-mYFP expression and interaction in cultured neonatal rabbit cardiomyocytes (NRbCM). A : 4–5 days postisolation, cultures have a myocyte purity of 80%, as evidenced by double immunofluorescent labeling for vimentin (fibroblasts,

    Techniques Used: Expressing, Cell Culture, Labeling

    Co-expression of paired KCNQ1 and hERG constructs and raw apFRET data demonstrate differences based on label placement. A–D : FRET pairs are indicated, representing the four possible combinations of fluorophore placement. Top : representative images
    Figure Legend Snippet: Co-expression of paired KCNQ1 and hERG constructs and raw apFRET data demonstrate differences based on label placement. A–D : FRET pairs are indicated, representing the four possible combinations of fluorophore placement. Top : representative images

    Techniques Used: Expressing, Construct

    apFRET demonstrates significant interactions between KCNQ1 and hERG fusion constructs. A : average FRET efficiencies for overexpression and positive controls as well as the four pairs varying KCNQ1 and hERG fluorophore placement. C and Y indicate mCFP
    Figure Legend Snippet: apFRET demonstrates significant interactions between KCNQ1 and hERG fusion constructs. A : average FRET efficiencies for overexpression and positive controls as well as the four pairs varying KCNQ1 and hERG fluorophore placement. C and Y indicate mCFP

    Techniques Used: Construct, Over Expression

    10) Product Images from "Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP"

    Article Title: Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    doi: 10.1152/ajpheart.00385.2012

    Increased intracellular cAMP reduces hERG-KCNQ1 interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP
    Figure Legend Snippet: Increased intracellular cAMP reduces hERG-KCNQ1 interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP

    Techniques Used: Construct, Labeling

    hERG-mCFP + KCNQ1-mYFP expression and interaction in cultured neonatal rabbit cardiomyocytes (NRbCM). A : 4–5 days postisolation, cultures have a myocyte purity of 80%, as evidenced by double immunofluorescent labeling for vimentin (fibroblasts,
    Figure Legend Snippet: hERG-mCFP + KCNQ1-mYFP expression and interaction in cultured neonatal rabbit cardiomyocytes (NRbCM). A : 4–5 days postisolation, cultures have a myocyte purity of 80%, as evidenced by double immunofluorescent labeling for vimentin (fibroblasts,

    Techniques Used: Expressing, Cell Culture, Labeling

    Co-expression of paired KCNQ1 and hERG constructs and raw apFRET data demonstrate differences based on label placement. A–D : FRET pairs are indicated, representing the four possible combinations of fluorophore placement. Top : representative images
    Figure Legend Snippet: Co-expression of paired KCNQ1 and hERG constructs and raw apFRET data demonstrate differences based on label placement. A–D : FRET pairs are indicated, representing the four possible combinations of fluorophore placement. Top : representative images

    Techniques Used: Expressing, Construct

    apFRET demonstrates significant interactions between KCNQ1 and hERG fusion constructs. A : average FRET efficiencies for overexpression and positive controls as well as the four pairs varying KCNQ1 and hERG fluorophore placement. C and Y indicate mCFP
    Figure Legend Snippet: apFRET demonstrates significant interactions between KCNQ1 and hERG fusion constructs. A : average FRET efficiencies for overexpression and positive controls as well as the four pairs varying KCNQ1 and hERG fluorophore placement. C and Y indicate mCFP

    Techniques Used: Construct, Over Expression

    11) Product Images from "Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP"

    Article Title: Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    doi: 10.1152/ajpheart.00385.2012

    Increased intracellular cAMP reduces hERG-KCNQ1 interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP
    Figure Legend Snippet: Increased intracellular cAMP reduces hERG-KCNQ1 interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP

    Techniques Used: Construct, Labeling

    hERG-mCFP + KCNQ1-mYFP expression and interaction in cultured neonatal rabbit cardiomyocytes (NRbCM). A : 4–5 days postisolation, cultures have a myocyte purity of 80%, as evidenced by double immunofluorescent labeling for vimentin (fibroblasts,
    Figure Legend Snippet: hERG-mCFP + KCNQ1-mYFP expression and interaction in cultured neonatal rabbit cardiomyocytes (NRbCM). A : 4–5 days postisolation, cultures have a myocyte purity of 80%, as evidenced by double immunofluorescent labeling for vimentin (fibroblasts,

    Techniques Used: Expressing, Cell Culture, Labeling

    Co-expression of paired KCNQ1 and hERG constructs and raw apFRET data demonstrate differences based on label placement. A–D : FRET pairs are indicated, representing the four possible combinations of fluorophore placement. Top : representative images
    Figure Legend Snippet: Co-expression of paired KCNQ1 and hERG constructs and raw apFRET data demonstrate differences based on label placement. A–D : FRET pairs are indicated, representing the four possible combinations of fluorophore placement. Top : representative images

    Techniques Used: Expressing, Construct

    apFRET demonstrates significant interactions between KCNQ1 and hERG fusion constructs. A : average FRET efficiencies for overexpression and positive controls as well as the four pairs varying KCNQ1 and hERG fluorophore placement. C and Y indicate mCFP
    Figure Legend Snippet: apFRET demonstrates significant interactions between KCNQ1 and hERG fusion constructs. A : average FRET efficiencies for overexpression and positive controls as well as the four pairs varying KCNQ1 and hERG fluorophore placement. C and Y indicate mCFP

    Techniques Used: Construct, Over Expression

    12) Product Images from "Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP"

    Article Title: Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    doi: 10.1152/ajpheart.00385.2012

    Increased intracellular cAMP reduces hERG-KCNQ1 interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP
    Figure Legend Snippet: Increased intracellular cAMP reduces hERG-KCNQ1 interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP

    Techniques Used: Construct, Labeling

    hERG-mCFP + KCNQ1-mYFP expression and interaction in cultured neonatal rabbit cardiomyocytes (NRbCM). A : 4–5 days postisolation, cultures have a myocyte purity of 80%, as evidenced by double immunofluorescent labeling for vimentin (fibroblasts,
    Figure Legend Snippet: hERG-mCFP + KCNQ1-mYFP expression and interaction in cultured neonatal rabbit cardiomyocytes (NRbCM). A : 4–5 days postisolation, cultures have a myocyte purity of 80%, as evidenced by double immunofluorescent labeling for vimentin (fibroblasts,

    Techniques Used: Expressing, Cell Culture, Labeling

    Co-expression of paired KCNQ1 and hERG constructs and raw apFRET data demonstrate differences based on label placement. A–D : FRET pairs are indicated, representing the four possible combinations of fluorophore placement. Top : representative images
    Figure Legend Snippet: Co-expression of paired KCNQ1 and hERG constructs and raw apFRET data demonstrate differences based on label placement. A–D : FRET pairs are indicated, representing the four possible combinations of fluorophore placement. Top : representative images

    Techniques Used: Expressing, Construct

    apFRET demonstrates significant interactions between KCNQ1 and hERG fusion constructs. A : average FRET efficiencies for overexpression and positive controls as well as the four pairs varying KCNQ1 and hERG fluorophore placement. C and Y indicate mCFP
    Figure Legend Snippet: apFRET demonstrates significant interactions between KCNQ1 and hERG fusion constructs. A : average FRET efficiencies for overexpression and positive controls as well as the four pairs varying KCNQ1 and hERG fluorophore placement. C and Y indicate mCFP

    Techniques Used: Construct, Over Expression

    13) Product Images from "Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP"

    Article Title: Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    doi: 10.1152/ajpheart.00385.2012

    Increased intracellular cAMP reduces hERG-KCNQ1 interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP
    Figure Legend Snippet: Increased intracellular cAMP reduces hERG-KCNQ1 interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP

    Techniques Used: Construct, Labeling

    hERG-mCFP + KCNQ1-mYFP expression and interaction in cultured neonatal rabbit cardiomyocytes (NRbCM). A : 4–5 days postisolation, cultures have a myocyte purity of 80%, as evidenced by double immunofluorescent labeling for vimentin (fibroblasts,
    Figure Legend Snippet: hERG-mCFP + KCNQ1-mYFP expression and interaction in cultured neonatal rabbit cardiomyocytes (NRbCM). A : 4–5 days postisolation, cultures have a myocyte purity of 80%, as evidenced by double immunofluorescent labeling for vimentin (fibroblasts,

    Techniques Used: Expressing, Cell Culture, Labeling

    Co-expression of paired KCNQ1 and hERG constructs and raw apFRET data demonstrate differences based on label placement. A–D : FRET pairs are indicated, representing the four possible combinations of fluorophore placement. Top : representative images
    Figure Legend Snippet: Co-expression of paired KCNQ1 and hERG constructs and raw apFRET data demonstrate differences based on label placement. A–D : FRET pairs are indicated, representing the four possible combinations of fluorophore placement. Top : representative images

    Techniques Used: Expressing, Construct

    apFRET demonstrates significant interactions between KCNQ1 and hERG fusion constructs. A : average FRET efficiencies for overexpression and positive controls as well as the four pairs varying KCNQ1 and hERG fluorophore placement. C and Y indicate mCFP
    Figure Legend Snippet: apFRET demonstrates significant interactions between KCNQ1 and hERG fusion constructs. A : average FRET efficiencies for overexpression and positive controls as well as the four pairs varying KCNQ1 and hERG fluorophore placement. C and Y indicate mCFP

    Techniques Used: Construct, Over Expression

    14) Product Images from "Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP"

    Article Title: Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    doi: 10.1152/ajpheart.00385.2012

    Increased intracellular cAMP reduces hERG-KCNQ1 interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP
    Figure Legend Snippet: Increased intracellular cAMP reduces hERG-KCNQ1 interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP

    Techniques Used: Construct, Labeling

    hERG-mCFP + KCNQ1-mYFP expression and interaction in cultured neonatal rabbit cardiomyocytes (NRbCM). A : 4–5 days postisolation, cultures have a myocyte purity of 80%, as evidenced by double immunofluorescent labeling for vimentin (fibroblasts,
    Figure Legend Snippet: hERG-mCFP + KCNQ1-mYFP expression and interaction in cultured neonatal rabbit cardiomyocytes (NRbCM). A : 4–5 days postisolation, cultures have a myocyte purity of 80%, as evidenced by double immunofluorescent labeling for vimentin (fibroblasts,

    Techniques Used: Expressing, Cell Culture, Labeling

    Co-expression of paired KCNQ1 and hERG constructs and raw apFRET data demonstrate differences based on label placement. A–D : FRET pairs are indicated, representing the four possible combinations of fluorophore placement. Top : representative images
    Figure Legend Snippet: Co-expression of paired KCNQ1 and hERG constructs and raw apFRET data demonstrate differences based on label placement. A–D : FRET pairs are indicated, representing the four possible combinations of fluorophore placement. Top : representative images

    Techniques Used: Expressing, Construct

    apFRET demonstrates significant interactions between KCNQ1 and hERG fusion constructs. A : average FRET efficiencies for overexpression and positive controls as well as the four pairs varying KCNQ1 and hERG fluorophore placement. C and Y indicate mCFP
    Figure Legend Snippet: apFRET demonstrates significant interactions between KCNQ1 and hERG fusion constructs. A : average FRET efficiencies for overexpression and positive controls as well as the four pairs varying KCNQ1 and hERG fluorophore placement. C and Y indicate mCFP

    Techniques Used: Construct, Over Expression

    15) Product Images from "Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP"

    Article Title: Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    doi: 10.1152/ajpheart.00385.2012

    Increased intracellular cAMP reduces hERG-KCNQ1 interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP
    Figure Legend Snippet: Increased intracellular cAMP reduces hERG-KCNQ1 interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP

    Techniques Used: Construct, Labeling

    hERG-mCFP + KCNQ1-mYFP expression and interaction in cultured neonatal rabbit cardiomyocytes (NRbCM). A : 4–5 days postisolation, cultures have a myocyte purity of 80%, as evidenced by double immunofluorescent labeling for vimentin (fibroblasts,
    Figure Legend Snippet: hERG-mCFP + KCNQ1-mYFP expression and interaction in cultured neonatal rabbit cardiomyocytes (NRbCM). A : 4–5 days postisolation, cultures have a myocyte purity of 80%, as evidenced by double immunofluorescent labeling for vimentin (fibroblasts,

    Techniques Used: Expressing, Cell Culture, Labeling

    Co-expression of paired KCNQ1 and hERG constructs and raw apFRET data demonstrate differences based on label placement. A–D : FRET pairs are indicated, representing the four possible combinations of fluorophore placement. Top : representative images
    Figure Legend Snippet: Co-expression of paired KCNQ1 and hERG constructs and raw apFRET data demonstrate differences based on label placement. A–D : FRET pairs are indicated, representing the four possible combinations of fluorophore placement. Top : representative images

    Techniques Used: Expressing, Construct

    apFRET demonstrates significant interactions between KCNQ1 and hERG fusion constructs. A : average FRET efficiencies for overexpression and positive controls as well as the four pairs varying KCNQ1 and hERG fluorophore placement. C and Y indicate mCFP
    Figure Legend Snippet: apFRET demonstrates significant interactions between KCNQ1 and hERG fusion constructs. A : average FRET efficiencies for overexpression and positive controls as well as the four pairs varying KCNQ1 and hERG fluorophore placement. C and Y indicate mCFP

    Techniques Used: Construct, Over Expression

    16) Product Images from "Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP"

    Article Title: Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    doi: 10.1152/ajpheart.00385.2012

    Increased intracellular cAMP reduces hERG-KCNQ1 interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP
    Figure Legend Snippet: Increased intracellular cAMP reduces hERG-KCNQ1 interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP

    Techniques Used: Construct, Labeling

    hERG-mCFP + KCNQ1-mYFP expression and interaction in cultured neonatal rabbit cardiomyocytes (NRbCM). A : 4–5 days postisolation, cultures have a myocyte purity of 80%, as evidenced by double immunofluorescent labeling for vimentin (fibroblasts,
    Figure Legend Snippet: hERG-mCFP + KCNQ1-mYFP expression and interaction in cultured neonatal rabbit cardiomyocytes (NRbCM). A : 4–5 days postisolation, cultures have a myocyte purity of 80%, as evidenced by double immunofluorescent labeling for vimentin (fibroblasts,

    Techniques Used: Expressing, Cell Culture, Labeling

    Co-expression of paired KCNQ1 and hERG constructs and raw apFRET data demonstrate differences based on label placement. A–D : FRET pairs are indicated, representing the four possible combinations of fluorophore placement. Top : representative images
    Figure Legend Snippet: Co-expression of paired KCNQ1 and hERG constructs and raw apFRET data demonstrate differences based on label placement. A–D : FRET pairs are indicated, representing the four possible combinations of fluorophore placement. Top : representative images

    Techniques Used: Expressing, Construct

    apFRET demonstrates significant interactions between KCNQ1 and hERG fusion constructs. A : average FRET efficiencies for overexpression and positive controls as well as the four pairs varying KCNQ1 and hERG fluorophore placement. C and Y indicate mCFP
    Figure Legend Snippet: apFRET demonstrates significant interactions between KCNQ1 and hERG fusion constructs. A : average FRET efficiencies for overexpression and positive controls as well as the four pairs varying KCNQ1 and hERG fluorophore placement. C and Y indicate mCFP

    Techniques Used: Construct, Over Expression

    17) Product Images from "Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP"

    Article Title: Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    doi: 10.1152/ajpheart.00385.2012

    Increased intracellular cAMP reduces hERG-KCNQ1 interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP
    Figure Legend Snippet: Increased intracellular cAMP reduces hERG-KCNQ1 interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP

    Techniques Used: Construct, Labeling

    hERG-mCFP + KCNQ1-mYFP expression and interaction in cultured neonatal rabbit cardiomyocytes (NRbCM). A : 4–5 days postisolation, cultures have a myocyte purity of 80%, as evidenced by double immunofluorescent labeling for vimentin (fibroblasts,
    Figure Legend Snippet: hERG-mCFP + KCNQ1-mYFP expression and interaction in cultured neonatal rabbit cardiomyocytes (NRbCM). A : 4–5 days postisolation, cultures have a myocyte purity of 80%, as evidenced by double immunofluorescent labeling for vimentin (fibroblasts,

    Techniques Used: Expressing, Cell Culture, Labeling

    Co-expression of paired KCNQ1 and hERG constructs and raw apFRET data demonstrate differences based on label placement. A–D : FRET pairs are indicated, representing the four possible combinations of fluorophore placement. Top : representative images
    Figure Legend Snippet: Co-expression of paired KCNQ1 and hERG constructs and raw apFRET data demonstrate differences based on label placement. A–D : FRET pairs are indicated, representing the four possible combinations of fluorophore placement. Top : representative images

    Techniques Used: Expressing, Construct

    apFRET demonstrates significant interactions between KCNQ1 and hERG fusion constructs. A : average FRET efficiencies for overexpression and positive controls as well as the four pairs varying KCNQ1 and hERG fluorophore placement. C and Y indicate mCFP
    Figure Legend Snippet: apFRET demonstrates significant interactions between KCNQ1 and hERG fusion constructs. A : average FRET efficiencies for overexpression and positive controls as well as the four pairs varying KCNQ1 and hERG fluorophore placement. C and Y indicate mCFP

    Techniques Used: Construct, Over Expression

    18) Product Images from "Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP"

    Article Title: Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    doi: 10.1152/ajpheart.00385.2012

    Increased intracellular cAMP reduces hERG-KCNQ1 interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP
    Figure Legend Snippet: Increased intracellular cAMP reduces hERG-KCNQ1 interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP

    Techniques Used: Construct, Labeling

    hERG-mCFP + KCNQ1-mYFP expression and interaction in cultured neonatal rabbit cardiomyocytes (NRbCM). A : 4–5 days postisolation, cultures have a myocyte purity of 80%, as evidenced by double immunofluorescent labeling for vimentin (fibroblasts,
    Figure Legend Snippet: hERG-mCFP + KCNQ1-mYFP expression and interaction in cultured neonatal rabbit cardiomyocytes (NRbCM). A : 4–5 days postisolation, cultures have a myocyte purity of 80%, as evidenced by double immunofluorescent labeling for vimentin (fibroblasts,

    Techniques Used: Expressing, Cell Culture, Labeling

    Co-expression of paired KCNQ1 and hERG constructs and raw apFRET data demonstrate differences based on label placement. A–D : FRET pairs are indicated, representing the four possible combinations of fluorophore placement. Top : representative images
    Figure Legend Snippet: Co-expression of paired KCNQ1 and hERG constructs and raw apFRET data demonstrate differences based on label placement. A–D : FRET pairs are indicated, representing the four possible combinations of fluorophore placement. Top : representative images

    Techniques Used: Expressing, Construct

    apFRET demonstrates significant interactions between KCNQ1 and hERG fusion constructs. A : average FRET efficiencies for overexpression and positive controls as well as the four pairs varying KCNQ1 and hERG fluorophore placement. C and Y indicate mCFP
    Figure Legend Snippet: apFRET demonstrates significant interactions between KCNQ1 and hERG fusion constructs. A : average FRET efficiencies for overexpression and positive controls as well as the four pairs varying KCNQ1 and hERG fluorophore placement. C and Y indicate mCFP

    Techniques Used: Construct, Over Expression

    19) Product Images from "Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP"

    Article Title: Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    doi: 10.1152/ajpheart.00385.2012

    Increased intracellular cAMP reduces hERG-KCNQ1 interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP
    Figure Legend Snippet: Increased intracellular cAMP reduces hERG-KCNQ1 interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP

    Techniques Used: Construct, Labeling

    hERG-mCFP + KCNQ1-mYFP expression and interaction in cultured neonatal rabbit cardiomyocytes (NRbCM). A : 4–5 days postisolation, cultures have a myocyte purity of 80%, as evidenced by double immunofluorescent labeling for vimentin (fibroblasts,
    Figure Legend Snippet: hERG-mCFP + KCNQ1-mYFP expression and interaction in cultured neonatal rabbit cardiomyocytes (NRbCM). A : 4–5 days postisolation, cultures have a myocyte purity of 80%, as evidenced by double immunofluorescent labeling for vimentin (fibroblasts,

    Techniques Used: Expressing, Cell Culture, Labeling

    Co-expression of paired KCNQ1 and hERG constructs and raw apFRET data demonstrate differences based on label placement. A–D : FRET pairs are indicated, representing the four possible combinations of fluorophore placement. Top : representative images
    Figure Legend Snippet: Co-expression of paired KCNQ1 and hERG constructs and raw apFRET data demonstrate differences based on label placement. A–D : FRET pairs are indicated, representing the four possible combinations of fluorophore placement. Top : representative images

    Techniques Used: Expressing, Construct

    apFRET demonstrates significant interactions between KCNQ1 and hERG fusion constructs. A : average FRET efficiencies for overexpression and positive controls as well as the four pairs varying KCNQ1 and hERG fluorophore placement. C and Y indicate mCFP
    Figure Legend Snippet: apFRET demonstrates significant interactions between KCNQ1 and hERG fusion constructs. A : average FRET efficiencies for overexpression and positive controls as well as the four pairs varying KCNQ1 and hERG fluorophore placement. C and Y indicate mCFP

    Techniques Used: Construct, Over Expression

    20) Product Images from "Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP"

    Article Title: Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    doi: 10.1152/ajpheart.00385.2012

    Increased intracellular cAMP reduces hERG-KCNQ1 interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP
    Figure Legend Snippet: Increased intracellular cAMP reduces hERG-KCNQ1 interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP

    Techniques Used: Construct, Labeling

    hERG-mCFP + KCNQ1-mYFP expression and interaction in cultured neonatal rabbit cardiomyocytes (NRbCM). A : 4–5 days postisolation, cultures have a myocyte purity of 80%, as evidenced by double immunofluorescent labeling for vimentin (fibroblasts,
    Figure Legend Snippet: hERG-mCFP + KCNQ1-mYFP expression and interaction in cultured neonatal rabbit cardiomyocytes (NRbCM). A : 4–5 days postisolation, cultures have a myocyte purity of 80%, as evidenced by double immunofluorescent labeling for vimentin (fibroblasts,

    Techniques Used: Expressing, Cell Culture, Labeling

    Co-expression of paired KCNQ1 and hERG constructs and raw apFRET data demonstrate differences based on label placement. A–D : FRET pairs are indicated, representing the four possible combinations of fluorophore placement. Top : representative images
    Figure Legend Snippet: Co-expression of paired KCNQ1 and hERG constructs and raw apFRET data demonstrate differences based on label placement. A–D : FRET pairs are indicated, representing the four possible combinations of fluorophore placement. Top : representative images

    Techniques Used: Expressing, Construct

    apFRET demonstrates significant interactions between KCNQ1 and hERG fusion constructs. A : average FRET efficiencies for overexpression and positive controls as well as the four pairs varying KCNQ1 and hERG fluorophore placement. C and Y indicate mCFP
    Figure Legend Snippet: apFRET demonstrates significant interactions between KCNQ1 and hERG fusion constructs. A : average FRET efficiencies for overexpression and positive controls as well as the four pairs varying KCNQ1 and hERG fluorophore placement. C and Y indicate mCFP

    Techniques Used: Construct, Over Expression

    21) Product Images from "Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP"

    Article Title: Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    doi: 10.1152/ajpheart.00385.2012

    Increased intracellular cAMP reduces hERG-KCNQ1 interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP
    Figure Legend Snippet: Increased intracellular cAMP reduces hERG-KCNQ1 interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP

    Techniques Used: Construct, Labeling

    hERG-mCFP + KCNQ1-mYFP expression and interaction in cultured neonatal rabbit cardiomyocytes (NRbCM). A : 4–5 days postisolation, cultures have a myocyte purity of 80%, as evidenced by double immunofluorescent labeling for vimentin (fibroblasts,
    Figure Legend Snippet: hERG-mCFP + KCNQ1-mYFP expression and interaction in cultured neonatal rabbit cardiomyocytes (NRbCM). A : 4–5 days postisolation, cultures have a myocyte purity of 80%, as evidenced by double immunofluorescent labeling for vimentin (fibroblasts,

    Techniques Used: Expressing, Cell Culture, Labeling

    Co-expression of paired KCNQ1 and hERG constructs and raw apFRET data demonstrate differences based on label placement. A–D : FRET pairs are indicated, representing the four possible combinations of fluorophore placement. Top : representative images
    Figure Legend Snippet: Co-expression of paired KCNQ1 and hERG constructs and raw apFRET data demonstrate differences based on label placement. A–D : FRET pairs are indicated, representing the four possible combinations of fluorophore placement. Top : representative images

    Techniques Used: Expressing, Construct

    apFRET demonstrates significant interactions between KCNQ1 and hERG fusion constructs. A : average FRET efficiencies for overexpression and positive controls as well as the four pairs varying KCNQ1 and hERG fluorophore placement. C and Y indicate mCFP
    Figure Legend Snippet: apFRET demonstrates significant interactions between KCNQ1 and hERG fusion constructs. A : average FRET efficiencies for overexpression and positive controls as well as the four pairs varying KCNQ1 and hERG fluorophore placement. C and Y indicate mCFP

    Techniques Used: Construct, Over Expression

    22) Product Images from "Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP"

    Article Title: Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    doi: 10.1152/ajpheart.00385.2012

    Increased intracellular cAMP reduces hERG-KCNQ1 interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP
    Figure Legend Snippet: Increased intracellular cAMP reduces hERG-KCNQ1 interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP

    Techniques Used: Construct, Labeling

    hERG-mCFP + KCNQ1-mYFP expression and interaction in cultured neonatal rabbit cardiomyocytes (NRbCM). A : 4–5 days postisolation, cultures have a myocyte purity of 80%, as evidenced by double immunofluorescent labeling for vimentin (fibroblasts,
    Figure Legend Snippet: hERG-mCFP + KCNQ1-mYFP expression and interaction in cultured neonatal rabbit cardiomyocytes (NRbCM). A : 4–5 days postisolation, cultures have a myocyte purity of 80%, as evidenced by double immunofluorescent labeling for vimentin (fibroblasts,

    Techniques Used: Expressing, Cell Culture, Labeling

    Co-expression of paired KCNQ1 and hERG constructs and raw apFRET data demonstrate differences based on label placement. A–D : FRET pairs are indicated, representing the four possible combinations of fluorophore placement. Top : representative images
    Figure Legend Snippet: Co-expression of paired KCNQ1 and hERG constructs and raw apFRET data demonstrate differences based on label placement. A–D : FRET pairs are indicated, representing the four possible combinations of fluorophore placement. Top : representative images

    Techniques Used: Expressing, Construct

    apFRET demonstrates significant interactions between KCNQ1 and hERG fusion constructs. A : average FRET efficiencies for overexpression and positive controls as well as the four pairs varying KCNQ1 and hERG fluorophore placement. C and Y indicate mCFP
    Figure Legend Snippet: apFRET demonstrates significant interactions between KCNQ1 and hERG fusion constructs. A : average FRET efficiencies for overexpression and positive controls as well as the four pairs varying KCNQ1 and hERG fluorophore placement. C and Y indicate mCFP

    Techniques Used: Construct, Over Expression

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    Increased intracellular cAMP reduces <t>hERG-KCNQ1</t> interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP
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    Increased intracellular cAMP reduces hERG-KCNQ1 interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    Article Title: Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP

    doi: 10.1152/ajpheart.00385.2012

    Figure Lengend Snippet: Increased intracellular cAMP reduces hERG-KCNQ1 interactions. A : average E f values for the positive FRET construct KCNQ1-CFP-YFP are unchanged following treatment with forskolin + IBMX ( P > 0.6). For the COOH-terminally labeled FRET pair (hERG-mCFP

    Article Snippet: However, it should be noted that NH2 -terminal fusions are prone to trafficking issues, and, for KCNQ1 in particular, confocal imaging demonstrated differences in protein location based on fluorophore placement ( and ).

    Techniques: Construct, Labeling

    hERG-mCFP + KCNQ1-mYFP expression and interaction in cultured neonatal rabbit cardiomyocytes (NRbCM). A : 4–5 days postisolation, cultures have a myocyte purity of 80%, as evidenced by double immunofluorescent labeling for vimentin (fibroblasts,

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    Article Title: Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP

    doi: 10.1152/ajpheart.00385.2012

    Figure Lengend Snippet: hERG-mCFP + KCNQ1-mYFP expression and interaction in cultured neonatal rabbit cardiomyocytes (NRbCM). A : 4–5 days postisolation, cultures have a myocyte purity of 80%, as evidenced by double immunofluorescent labeling for vimentin (fibroblasts,

    Article Snippet: However, it should be noted that NH2 -terminal fusions are prone to trafficking issues, and, for KCNQ1 in particular, confocal imaging demonstrated differences in protein location based on fluorophore placement ( and ).

    Techniques: Expressing, Cell Culture, Labeling

    Co-expression of paired KCNQ1 and hERG constructs and raw apFRET data demonstrate differences based on label placement. A–D : FRET pairs are indicated, representing the four possible combinations of fluorophore placement. Top : representative images

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    Article Title: Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP

    doi: 10.1152/ajpheart.00385.2012

    Figure Lengend Snippet: Co-expression of paired KCNQ1 and hERG constructs and raw apFRET data demonstrate differences based on label placement. A–D : FRET pairs are indicated, representing the four possible combinations of fluorophore placement. Top : representative images

    Article Snippet: However, it should be noted that NH2 -terminal fusions are prone to trafficking issues, and, for KCNQ1 in particular, confocal imaging demonstrated differences in protein location based on fluorophore placement ( and ).

    Techniques: Expressing, Construct

    apFRET demonstrates significant interactions between KCNQ1 and hERG fusion constructs. A : average FRET efficiencies for overexpression and positive controls as well as the four pairs varying KCNQ1 and hERG fluorophore placement. C and Y indicate mCFP

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    Article Title: Interactions between hERG and KCNQ1 ?-subunits are mediated by their COOH termini and modulated by cAMP

    doi: 10.1152/ajpheart.00385.2012

    Figure Lengend Snippet: apFRET demonstrates significant interactions between KCNQ1 and hERG fusion constructs. A : average FRET efficiencies for overexpression and positive controls as well as the four pairs varying KCNQ1 and hERG fluorophore placement. C and Y indicate mCFP

    Article Snippet: However, it should be noted that NH2 -terminal fusions are prone to trafficking issues, and, for KCNQ1 in particular, confocal imaging demonstrated differences in protein location based on fluorophore placement ( and ).

    Techniques: Construct, Over Expression