rnase t1  (Worthington Biochemical)


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    Name:
    Ribonuclease T1 Chromatographically Purif Lyophilized
    Description:
    Highly purified microbial non mammalian RNase prepared with non animal components Supplied as a lyophilized powder
    Catalog Number:
    ls01490
    Price:
    151
    Size:
    500 ku
    Source:
    Aspergillus oryzae
    Cas Number:
    9026.12.4
    Buy from Supplier


    Structured Review

    Worthington Biochemical rnase t1
    CID-based sequencing of the double carbodiimide-tagged <t>RNase</t> T1 digestion product from the anticodon of E. coli tRNA Tyr(QUA) . Shown is the CID MS/MS spectrum of ACU[Q]UA[ms 2 i 6 A]AΨCUG with two carbodiimide units (+502 Da, m / z 1506.6). The appearance
    Highly purified microbial non mammalian RNase prepared with non animal components Supplied as a lyophilized powder
    https://www.bioz.com/result/rnase t1/product/Worthington Biochemical
    Average 94 stars, based on 82 article reviews
    Price from $9.99 to $1999.99
    rnase t1 - by Bioz Stars, 2021-01
    94/100 stars

    Images

    1) Product Images from "Pseudouridine in the Anticodon of Escherichia coli tRNATyr(QΨA) Is Catalyzed by the Dual Specificity Enzyme RluF *"

    Article Title: Pseudouridine in the Anticodon of Escherichia coli tRNATyr(QΨA) Is Catalyzed by the Dual Specificity Enzyme RluF *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M116.747865

    CID-based sequencing of the double carbodiimide-tagged RNase T1 digestion product from the anticodon of E. coli tRNA Tyr(QUA) . Shown is the CID MS/MS spectrum of ACU[Q]UA[ms 2 i 6 A]AΨCUG with two carbodiimide units (+502 Da, m / z 1506.6). The appearance
    Figure Legend Snippet: CID-based sequencing of the double carbodiimide-tagged RNase T1 digestion product from the anticodon of E. coli tRNA Tyr(QUA) . Shown is the CID MS/MS spectrum of ACU[Q]UA[ms 2 i 6 A]AΨCUG with two carbodiimide units (+502 Da, m / z 1506.6). The appearance

    Techniques Used: Sequencing, Mass Spectrometry

    LC-MS analysis of the carbodiimide-tagged RNase T1 digestion product the from anticodon of E. coli tRNA Tyr(QUA) . A , total ion chromatogram representing the elution pattern of all the oligonucleotide digestion products detected as anions. B , XIC for m
    Figure Legend Snippet: LC-MS analysis of the carbodiimide-tagged RNase T1 digestion product the from anticodon of E. coli tRNA Tyr(QUA) . A , total ion chromatogram representing the elution pattern of all the oligonucleotide digestion products detected as anions. B , XIC for m

    Techniques Used: Liquid Chromatography with Mass Spectroscopy

    CID-based sequencing of the single carbodiimide-tagged RNase T1 digestion product the from anticodon of E. coli tRNA Tyr(QUA) . Shown is the CID MS/MS spectrum of ACU[Q]UA[ms 2 i 6 A]AΨCUG with one carbodiimide ( m / z 1422.8) tagged at either one of two
    Figure Legend Snippet: CID-based sequencing of the single carbodiimide-tagged RNase T1 digestion product the from anticodon of E. coli tRNA Tyr(QUA) . Shown is the CID MS/MS spectrum of ACU[Q]UA[ms 2 i 6 A]AΨCUG with one carbodiimide ( m / z 1422.8) tagged at either one of two

    Techniques Used: Sequencing, Mass Spectrometry

    LC-MS analysis of acrylonitrile-derivatized RNase T1/BAP digests of E. coli 23S rRNA. A , XIC for m / z 1305.2 corresponding to [Ψ][Ψ]CG with two cyanoethylations. B , mass spectrum corresponding to XIC peak at 23.4 min showing the presence
    Figure Legend Snippet: LC-MS analysis of acrylonitrile-derivatized RNase T1/BAP digests of E. coli 23S rRNA. A , XIC for m / z 1305.2 corresponding to [Ψ][Ψ]CG with two cyanoethylations. B , mass spectrum corresponding to XIC peak at 23.4 min showing the presence

    Techniques Used: Liquid Chromatography with Mass Spectroscopy

    LC-MS/MS Analysis of RNase T1 Digest of E. coli tRNATyr
    Figure Legend Snippet: LC-MS/MS Analysis of RNase T1 Digest of E. coli tRNATyr

    Techniques Used: Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry

    CID-based sequencing of the acrylonitrile-tagged RNase T1 digestion product the from anticodon of E. coli tDNA Tyr transcript. A , MS/MS spectrum of the doubly charged oligonucleotide ion originating from tDNA Tyr transcript treated with recombinant RluF
    Figure Legend Snippet: CID-based sequencing of the acrylonitrile-tagged RNase T1 digestion product the from anticodon of E. coli tDNA Tyr transcript. A , MS/MS spectrum of the doubly charged oligonucleotide ion originating from tDNA Tyr transcript treated with recombinant RluF

    Techniques Used: Sequencing, Mass Spectrometry, Recombinant

    2) Product Images from "Pseudouridine in the Anticodon of Escherichia coli tRNATyr(QΨA) Is Catalyzed by the Dual Specificity Enzyme RluF *"

    Article Title: Pseudouridine in the Anticodon of Escherichia coli tRNATyr(QΨA) Is Catalyzed by the Dual Specificity Enzyme RluF *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M116.747865

    CID-based sequencing of the double carbodiimide-tagged RNase T1 digestion product from the anticodon of E. coli tRNA Tyr(QUA) . Shown is the CID MS/MS spectrum of ACU[Q]UA[ms 2 i 6 A]AΨCUG with two carbodiimide units (+502 Da, m / z 1506.6). The appearance
    Figure Legend Snippet: CID-based sequencing of the double carbodiimide-tagged RNase T1 digestion product from the anticodon of E. coli tRNA Tyr(QUA) . Shown is the CID MS/MS spectrum of ACU[Q]UA[ms 2 i 6 A]AΨCUG with two carbodiimide units (+502 Da, m / z 1506.6). The appearance

    Techniques Used: Sequencing, Mass Spectrometry

    LC-MS analysis of the carbodiimide-tagged RNase T1 digestion product the from anticodon of E. coli tRNA Tyr(QUA) . A , total ion chromatogram representing the elution pattern of all the oligonucleotide digestion products detected as anions. B , XIC for m
    Figure Legend Snippet: LC-MS analysis of the carbodiimide-tagged RNase T1 digestion product the from anticodon of E. coli tRNA Tyr(QUA) . A , total ion chromatogram representing the elution pattern of all the oligonucleotide digestion products detected as anions. B , XIC for m

    Techniques Used: Liquid Chromatography with Mass Spectroscopy

    CID-based sequencing of the single carbodiimide-tagged RNase T1 digestion product the from anticodon of E. coli tRNA Tyr(QUA) . Shown is the CID MS/MS spectrum of ACU[Q]UA[ms 2 i 6 A]AΨCUG with one carbodiimide ( m / z 1422.8) tagged at either one of two
    Figure Legend Snippet: CID-based sequencing of the single carbodiimide-tagged RNase T1 digestion product the from anticodon of E. coli tRNA Tyr(QUA) . Shown is the CID MS/MS spectrum of ACU[Q]UA[ms 2 i 6 A]AΨCUG with one carbodiimide ( m / z 1422.8) tagged at either one of two

    Techniques Used: Sequencing, Mass Spectrometry

    Related Articles

    other:

    Article Title: Isolation and functional characterization of P-TEFb-associated factors that control general and HIV-1 transcriptional elongation
    Article Snippet: Add 3 μL RNase T1 (1 unit/μL in buffer D0.1; Worthington Biochemical Corp., Cat. no.: LS01485) to each reaction, mix well and incubate for 5 min at 37 °C.

    Purification:

    Article Title: Ariadne: a database search engine for identification and chemical analysis of RNA using tandem mass spectrometry data
    Article Snippet: .. Ribonuclease T1 (RNase T1; highly purified) was purchased from Worthington (Lakewood, NJ, USA) and purified by reversed-phase LC. .. In vitro transcription of mRNAs The coding region of Xenopus cyclophilin A (xCyPA) cDNA (NM_001089190.1) was inserted into pBluescript RN3 vector ( ) (provided by Dr Makoto Asashima of The University of Tokyo) at BglII and EcoRI sites downstream of the T3 promoter.

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