rnase t1  (Worthington Biochemical)


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    • 94
    Name:
    Phosphodiesterase I
    Description:
    Purified by the method of Williams Sung and Laskowski J Biol Chem 236 1130 1961 Further treated to inactivate contaminating 5 nucleotidase activity according to Sulkowski and Laskowski Biochim Biophys Acta 240 443 1961 Lyophilized in vials
    Catalog Number:
    ls003926
    Price:
    79
    Size:
    100 un
    Source:
    Crotalus adamanteus Venom
    Cas Number:
    9025.82.5
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    Structured Review

    Worthington Biochemical rnase t1
    CID-based sequencing of the double carbodiimide-tagged <t>RNase</t> T1 digestion product from the anticodon of E. coli tRNA Tyr(QUA) . Shown is the CID MS/MS spectrum of ACU[Q]UA[ms 2 i 6 A]AΨCUG with two carbodiimide units (+502 Da, m / z 1506.6). The appearance
    Purified by the method of Williams Sung and Laskowski J Biol Chem 236 1130 1961 Further treated to inactivate contaminating 5 nucleotidase activity according to Sulkowski and Laskowski Biochim Biophys Acta 240 443 1961 Lyophilized in vials
    https://www.bioz.com/result/rnase t1/product/Worthington Biochemical
    Average 94 stars, based on 82 article reviews
    Price from $9.99 to $1999.99
    rnase t1 - by Bioz Stars, 2020-05
    94/100 stars

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    Images

    1) Product Images from "Pseudouridine in the Anticodon of Escherichia coli tRNATyr(QΨA) Is Catalyzed by the Dual Specificity Enzyme RluF *"

    Article Title: Pseudouridine in the Anticodon of Escherichia coli tRNATyr(QΨA) Is Catalyzed by the Dual Specificity Enzyme RluF *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M116.747865

    CID-based sequencing of the double carbodiimide-tagged RNase T1 digestion product from the anticodon of E. coli tRNA Tyr(QUA) . Shown is the CID MS/MS spectrum of ACU[Q]UA[ms 2 i 6 A]AΨCUG with two carbodiimide units (+502 Da, m / z 1506.6). The appearance
    Figure Legend Snippet: CID-based sequencing of the double carbodiimide-tagged RNase T1 digestion product from the anticodon of E. coli tRNA Tyr(QUA) . Shown is the CID MS/MS spectrum of ACU[Q]UA[ms 2 i 6 A]AΨCUG with two carbodiimide units (+502 Da, m / z 1506.6). The appearance

    Techniques Used: Sequencing, Mass Spectrometry

    LC-MS analysis of the carbodiimide-tagged RNase T1 digestion product the from anticodon of E. coli tRNA Tyr(QUA) . A , total ion chromatogram representing the elution pattern of all the oligonucleotide digestion products detected as anions. B , XIC for m
    Figure Legend Snippet: LC-MS analysis of the carbodiimide-tagged RNase T1 digestion product the from anticodon of E. coli tRNA Tyr(QUA) . A , total ion chromatogram representing the elution pattern of all the oligonucleotide digestion products detected as anions. B , XIC for m

    Techniques Used: Liquid Chromatography with Mass Spectroscopy

    CID-based sequencing of the single carbodiimide-tagged RNase T1 digestion product the from anticodon of E. coli tRNA Tyr(QUA) . Shown is the CID MS/MS spectrum of ACU[Q]UA[ms 2 i 6 A]AΨCUG with one carbodiimide ( m / z 1422.8) tagged at either one of two
    Figure Legend Snippet: CID-based sequencing of the single carbodiimide-tagged RNase T1 digestion product the from anticodon of E. coli tRNA Tyr(QUA) . Shown is the CID MS/MS spectrum of ACU[Q]UA[ms 2 i 6 A]AΨCUG with one carbodiimide ( m / z 1422.8) tagged at either one of two

    Techniques Used: Sequencing, Mass Spectrometry

    LC-MS analysis of acrylonitrile-derivatized RNase T1/BAP digests of E. coli 23S rRNA. A , XIC for m / z 1305.2 corresponding to [Ψ][Ψ]CG with two cyanoethylations. B , mass spectrum corresponding to XIC peak at 23.4 min showing the presence
    Figure Legend Snippet: LC-MS analysis of acrylonitrile-derivatized RNase T1/BAP digests of E. coli 23S rRNA. A , XIC for m / z 1305.2 corresponding to [Ψ][Ψ]CG with two cyanoethylations. B , mass spectrum corresponding to XIC peak at 23.4 min showing the presence

    Techniques Used: Liquid Chromatography with Mass Spectroscopy

    LC-MS/MS Analysis of RNase T1 Digest of E. coli tRNATyr
    Figure Legend Snippet: LC-MS/MS Analysis of RNase T1 Digest of E. coli tRNATyr

    Techniques Used: Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry

    CID-based sequencing of the acrylonitrile-tagged RNase T1 digestion product the from anticodon of E. coli tDNA Tyr transcript. A , MS/MS spectrum of the doubly charged oligonucleotide ion originating from tDNA Tyr transcript treated with recombinant RluF
    Figure Legend Snippet: CID-based sequencing of the acrylonitrile-tagged RNase T1 digestion product the from anticodon of E. coli tDNA Tyr transcript. A , MS/MS spectrum of the doubly charged oligonucleotide ion originating from tDNA Tyr transcript treated with recombinant RluF

    Techniques Used: Sequencing, Mass Spectrometry, Recombinant

    2) Product Images from "Pseudouridine in the Anticodon of Escherichia coli tRNATyr(QΨA) Is Catalyzed by the Dual Specificity Enzyme RluF *"

    Article Title: Pseudouridine in the Anticodon of Escherichia coli tRNATyr(QΨA) Is Catalyzed by the Dual Specificity Enzyme RluF *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M116.747865

    CID-based sequencing of the double carbodiimide-tagged RNase T1 digestion product from the anticodon of E. coli tRNA Tyr(QUA) . Shown is the CID MS/MS spectrum of ACU[Q]UA[ms 2 i 6 A]AΨCUG with two carbodiimide units (+502 Da, m / z 1506.6). The appearance
    Figure Legend Snippet: CID-based sequencing of the double carbodiimide-tagged RNase T1 digestion product from the anticodon of E. coli tRNA Tyr(QUA) . Shown is the CID MS/MS spectrum of ACU[Q]UA[ms 2 i 6 A]AΨCUG with two carbodiimide units (+502 Da, m / z 1506.6). The appearance

    Techniques Used: Sequencing, Mass Spectrometry

    LC-MS analysis of the carbodiimide-tagged RNase T1 digestion product the from anticodon of E. coli tRNA Tyr(QUA) . A , total ion chromatogram representing the elution pattern of all the oligonucleotide digestion products detected as anions. B , XIC for m
    Figure Legend Snippet: LC-MS analysis of the carbodiimide-tagged RNase T1 digestion product the from anticodon of E. coli tRNA Tyr(QUA) . A , total ion chromatogram representing the elution pattern of all the oligonucleotide digestion products detected as anions. B , XIC for m

    Techniques Used: Liquid Chromatography with Mass Spectroscopy

    CID-based sequencing of the single carbodiimide-tagged RNase T1 digestion product the from anticodon of E. coli tRNA Tyr(QUA) . Shown is the CID MS/MS spectrum of ACU[Q]UA[ms 2 i 6 A]AΨCUG with one carbodiimide ( m / z 1422.8) tagged at either one of two
    Figure Legend Snippet: CID-based sequencing of the single carbodiimide-tagged RNase T1 digestion product the from anticodon of E. coli tRNA Tyr(QUA) . Shown is the CID MS/MS spectrum of ACU[Q]UA[ms 2 i 6 A]AΨCUG with one carbodiimide ( m / z 1422.8) tagged at either one of two

    Techniques Used: Sequencing, Mass Spectrometry

    Related Articles

    Concentration Assay:

    Article Title: Solution Structure of the B3 DNA Binding Domain of the Arabidopsis Cold-Responsive Transcription Factor RAV1 W⃞
    Article Snippet: .. The concentration of the double-stranded DNA was determined using an extinction coefficient calculated after digestion of the strands with phosphodiesterase I (Worthington Biochemical, Lakewood, NJ). .. The HOMOLOGY module of Insight II (Accelrys, San Diego, CA) was used for the structural modeling of the ARF1- and ABI3-B3 domains based on the sequence homology to RAV1-B3.

    Incubation:

    Article Title: Functional characterization of the YmcB and YqeV tRNA methylthiotransferases of Bacillus subtilis
    Article Snippet: .. Four microliter 0.1 M ammonium acetate pH 5.3 and 8 U Nuclease P1 (Sigma-Aldrich) were added, and samples were incubated at 45°C for 2 h. Four microliters of 0.1 M ammonium bicarbonate and 0.1 U Phosphodiesterase I (Worthington Biochemical, Lakewood, NJ, USA) were added, and samples were incubated at 37°C for 2 h. Five units Antarctic phosphatase (New England Biolabs) were added, and samples were incubated at 37°C for 1 h. .. Liquid chromatography and ESI-MS analysis Nucleosides were separated, at room temperature, on a Hitachi HPLC system (L-7100 pump) with UV detection at 260 nm (L-7400 UV detector).

    Article Title: Selective alkylation of T–T mismatched DNA using vinyldiaminotriazine–acridine conjugate
    Article Snippet: .. A solution (100 μl) of the alkylated ODN 5 or ODN 6 (100 μM), alkaline phosphatase (0.03 U/μl, Takara Bio Inc.) and phosphodiesterase I (0.03 U/μl, Worthington Biochemical Corp.) in alkaline phosphatase buffer was incubated at 37°C for 1 h. The reaction mixture was heated at 90°C for 3 min, cooled to 0°C for 3 min and analyzed by RP-HPLC (CAPCELL PAL MG-II, Shiseido, 4.6 × 250 mm, solvent A: 50 mM TEAA for analysis or HCOONH4 for purification, solvent B: MeCN, linear gradient: B 5–40%/ 30 min, flow rate: 1.0 ml/min). .. The alkylated thymidine dT* obtained by enzymatic hydrolysis of the alkylated ODN 5 and ODN 6 was dissolved in DMSO-d6 containing 10 μM 4,4-dimethyl-4-silapentane-1-sulfonic acid (DSS), the final concentration being 400 μM.

    other:

    Article Title: Mono(ADP-ribosyl)ation of 2?-deoxyguanosine residue in DNA by an apoptosis-inducing protein, pierisin-1, from cabbage butterfly
    Article Snippet: Micrococcal nuclease and phosphodiesterase I ( Crotalus adamanteus venom) and II (bovine spleen) were obtained from Worthington. β-[ Adenylate -32 P]NAD ([32 P]NAD) was purchased from NEN.

    Flow Cytometry:

    Article Title: Selective alkylation of T–T mismatched DNA using vinyldiaminotriazine–acridine conjugate
    Article Snippet: .. A solution (100 μl) of the alkylated ODN 5 or ODN 6 (100 μM), alkaline phosphatase (0.03 U/μl, Takara Bio Inc.) and phosphodiesterase I (0.03 U/μl, Worthington Biochemical Corp.) in alkaline phosphatase buffer was incubated at 37°C for 1 h. The reaction mixture was heated at 90°C for 3 min, cooled to 0°C for 3 min and analyzed by RP-HPLC (CAPCELL PAL MG-II, Shiseido, 4.6 × 250 mm, solvent A: 50 mM TEAA for analysis or HCOONH4 for purification, solvent B: MeCN, linear gradient: B 5–40%/ 30 min, flow rate: 1.0 ml/min). .. The alkylated thymidine dT* obtained by enzymatic hydrolysis of the alkylated ODN 5 and ODN 6 was dissolved in DMSO-d6 containing 10 μM 4,4-dimethyl-4-silapentane-1-sulfonic acid (DSS), the final concentration being 400 μM.

    Purification:

    Article Title: Selective alkylation of T–T mismatched DNA using vinyldiaminotriazine–acridine conjugate
    Article Snippet: .. A solution (100 μl) of the alkylated ODN 5 or ODN 6 (100 μM), alkaline phosphatase (0.03 U/μl, Takara Bio Inc.) and phosphodiesterase I (0.03 U/μl, Worthington Biochemical Corp.) in alkaline phosphatase buffer was incubated at 37°C for 1 h. The reaction mixture was heated at 90°C for 3 min, cooled to 0°C for 3 min and analyzed by RP-HPLC (CAPCELL PAL MG-II, Shiseido, 4.6 × 250 mm, solvent A: 50 mM TEAA for analysis or HCOONH4 for purification, solvent B: MeCN, linear gradient: B 5–40%/ 30 min, flow rate: 1.0 ml/min). .. The alkylated thymidine dT* obtained by enzymatic hydrolysis of the alkylated ODN 5 and ODN 6 was dissolved in DMSO-d6 containing 10 μM 4,4-dimethyl-4-silapentane-1-sulfonic acid (DSS), the final concentration being 400 μM.

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