rnase t1  (Worthington Biochemical)


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    Worthington Biochemical rnase t1
    CID-based sequencing of the double carbodiimide-tagged <t>RNase</t> T1 digestion product from the anticodon of E. coli tRNA Tyr(QUA) . Shown is the CID MS/MS spectrum of ACU[Q]UA[ms 2 i 6 A]AΨCUG with two carbodiimide units (+502 Da, m / z 1506.6). The appearance
    Rnase T1, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 94/100, based on 82 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnase t1/product/Worthington Biochemical
    Average 94 stars, based on 82 article reviews
    Price from $9.99 to $1999.99
    rnase t1 - by Bioz Stars, 2020-08
    94/100 stars

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    rnase v1
    rna
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    acn
    iodoacetamide iaa
    trifluoroacetic acid
    trifluoroacetic acid tfa
    acetonitrile acn

    Images

    1) Product Images from "Pseudouridine in the Anticodon of Escherichia coli tRNATyr(QΨA) Is Catalyzed by the Dual Specificity Enzyme RluF *"

    Article Title: Pseudouridine in the Anticodon of Escherichia coli tRNATyr(QΨA) Is Catalyzed by the Dual Specificity Enzyme RluF *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M116.747865

    CID-based sequencing of the double carbodiimide-tagged RNase T1 digestion product from the anticodon of E. coli tRNA Tyr(QUA) . Shown is the CID MS/MS spectrum of ACU[Q]UA[ms 2 i 6 A]AΨCUG with two carbodiimide units (+502 Da, m / z 1506.6). The appearance
    Figure Legend Snippet: CID-based sequencing of the double carbodiimide-tagged RNase T1 digestion product from the anticodon of E. coli tRNA Tyr(QUA) . Shown is the CID MS/MS spectrum of ACU[Q]UA[ms 2 i 6 A]AΨCUG with two carbodiimide units (+502 Da, m / z 1506.6). The appearance

    Techniques Used: Sequencing, Mass Spectrometry

    LC-MS analysis of the carbodiimide-tagged RNase T1 digestion product the from anticodon of E. coli tRNA Tyr(QUA) . A , total ion chromatogram representing the elution pattern of all the oligonucleotide digestion products detected as anions. B , XIC for m
    Figure Legend Snippet: LC-MS analysis of the carbodiimide-tagged RNase T1 digestion product the from anticodon of E. coli tRNA Tyr(QUA) . A , total ion chromatogram representing the elution pattern of all the oligonucleotide digestion products detected as anions. B , XIC for m

    Techniques Used: Liquid Chromatography with Mass Spectroscopy

    CID-based sequencing of the single carbodiimide-tagged RNase T1 digestion product the from anticodon of E. coli tRNA Tyr(QUA) . Shown is the CID MS/MS spectrum of ACU[Q]UA[ms 2 i 6 A]AΨCUG with one carbodiimide ( m / z 1422.8) tagged at either one of two
    Figure Legend Snippet: CID-based sequencing of the single carbodiimide-tagged RNase T1 digestion product the from anticodon of E. coli tRNA Tyr(QUA) . Shown is the CID MS/MS spectrum of ACU[Q]UA[ms 2 i 6 A]AΨCUG with one carbodiimide ( m / z 1422.8) tagged at either one of two

    Techniques Used: Sequencing, Mass Spectrometry

    LC-MS analysis of acrylonitrile-derivatized RNase T1/BAP digests of E. coli 23S rRNA. A , XIC for m / z 1305.2 corresponding to [Ψ][Ψ]CG with two cyanoethylations. B , mass spectrum corresponding to XIC peak at 23.4 min showing the presence
    Figure Legend Snippet: LC-MS analysis of acrylonitrile-derivatized RNase T1/BAP digests of E. coli 23S rRNA. A , XIC for m / z 1305.2 corresponding to [Ψ][Ψ]CG with two cyanoethylations. B , mass spectrum corresponding to XIC peak at 23.4 min showing the presence

    Techniques Used: Liquid Chromatography with Mass Spectroscopy

    LC-MS/MS Analysis of RNase T1 Digest of E. coli tRNATyr
    Figure Legend Snippet: LC-MS/MS Analysis of RNase T1 Digest of E. coli tRNATyr

    Techniques Used: Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry

    CID-based sequencing of the acrylonitrile-tagged RNase T1 digestion product the from anticodon of E. coli tDNA Tyr transcript. A , MS/MS spectrum of the doubly charged oligonucleotide ion originating from tDNA Tyr transcript treated with recombinant RluF
    Figure Legend Snippet: CID-based sequencing of the acrylonitrile-tagged RNase T1 digestion product the from anticodon of E. coli tDNA Tyr transcript. A , MS/MS spectrum of the doubly charged oligonucleotide ion originating from tDNA Tyr transcript treated with recombinant RluF

    Techniques Used: Sequencing, Mass Spectrometry, Recombinant

    2) Product Images from "Pseudouridine in the Anticodon of Escherichia coli tRNATyr(QΨA) Is Catalyzed by the Dual Specificity Enzyme RluF *"

    Article Title: Pseudouridine in the Anticodon of Escherichia coli tRNATyr(QΨA) Is Catalyzed by the Dual Specificity Enzyme RluF *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M116.747865

    CID-based sequencing of the double carbodiimide-tagged RNase T1 digestion product from the anticodon of E. coli tRNA Tyr(QUA) . Shown is the CID MS/MS spectrum of ACU[Q]UA[ms 2 i 6 A]AΨCUG with two carbodiimide units (+502 Da, m / z 1506.6). The appearance
    Figure Legend Snippet: CID-based sequencing of the double carbodiimide-tagged RNase T1 digestion product from the anticodon of E. coli tRNA Tyr(QUA) . Shown is the CID MS/MS spectrum of ACU[Q]UA[ms 2 i 6 A]AΨCUG with two carbodiimide units (+502 Da, m / z 1506.6). The appearance

    Techniques Used: Sequencing, Mass Spectrometry

    LC-MS analysis of the carbodiimide-tagged RNase T1 digestion product the from anticodon of E. coli tRNA Tyr(QUA) . A , total ion chromatogram representing the elution pattern of all the oligonucleotide digestion products detected as anions. B , XIC for m
    Figure Legend Snippet: LC-MS analysis of the carbodiimide-tagged RNase T1 digestion product the from anticodon of E. coli tRNA Tyr(QUA) . A , total ion chromatogram representing the elution pattern of all the oligonucleotide digestion products detected as anions. B , XIC for m

    Techniques Used: Liquid Chromatography with Mass Spectroscopy

    CID-based sequencing of the single carbodiimide-tagged RNase T1 digestion product the from anticodon of E. coli tRNA Tyr(QUA) . Shown is the CID MS/MS spectrum of ACU[Q]UA[ms 2 i 6 A]AΨCUG with one carbodiimide ( m / z 1422.8) tagged at either one of two
    Figure Legend Snippet: CID-based sequencing of the single carbodiimide-tagged RNase T1 digestion product the from anticodon of E. coli tRNA Tyr(QUA) . Shown is the CID MS/MS spectrum of ACU[Q]UA[ms 2 i 6 A]AΨCUG with one carbodiimide ( m / z 1422.8) tagged at either one of two

    Techniques Used: Sequencing, Mass Spectrometry

    Related Articles

    Concentration Assay:

    Article Title: Solution Structure of the B3 DNA Binding Domain of the Arabidopsis Cold-Responsive Transcription Factor RAV1 W⃞
    Article Snippet: .. The concentration of the double-stranded DNA was determined using an extinction coefficient calculated after digestion of the strands with phosphodiesterase I (Worthington Biochemical, Lakewood, NJ). .. The HOMOLOGY module of Insight II (Accelrys, San Diego, CA) was used for the structural modeling of the ARF1- and ABI3-B3 domains based on the sequence homology to RAV1-B3.

    Incubation:

    Article Title: Functional characterization of the YmcB and YqeV tRNA methylthiotransferases of Bacillus subtilis
    Article Snippet: .. Four microliter 0.1 M ammonium acetate pH 5.3 and 8 U Nuclease P1 (Sigma-Aldrich) were added, and samples were incubated at 45°C for 2 h. Four microliters of 0.1 M ammonium bicarbonate and 0.1 U Phosphodiesterase I (Worthington Biochemical, Lakewood, NJ, USA) were added, and samples were incubated at 37°C for 2 h. Five units Antarctic phosphatase (New England Biolabs) were added, and samples were incubated at 37°C for 1 h. .. Liquid chromatography and ESI-MS analysis Nucleosides were separated, at room temperature, on a Hitachi HPLC system (L-7100 pump) with UV detection at 260 nm (L-7400 UV detector).

    Article Title: Selective alkylation of T–T mismatched DNA using vinyldiaminotriazine–acridine conjugate
    Article Snippet: .. A solution (100 μl) of the alkylated ODN 5 or ODN 6 (100 μM), alkaline phosphatase (0.03 U/μl, Takara Bio Inc.) and phosphodiesterase I (0.03 U/μl, Worthington Biochemical Corp.) in alkaline phosphatase buffer was incubated at 37°C for 1 h. The reaction mixture was heated at 90°C for 3 min, cooled to 0°C for 3 min and analyzed by RP-HPLC (CAPCELL PAL MG-II, Shiseido, 4.6 × 250 mm, solvent A: 50 mM TEAA for analysis or HCOONH4 for purification, solvent B: MeCN, linear gradient: B 5–40%/ 30 min, flow rate: 1.0 ml/min). .. The alkylated thymidine dT* obtained by enzymatic hydrolysis of the alkylated ODN 5 and ODN 6 was dissolved in DMSO-d6 containing 10 μM 4,4-dimethyl-4-silapentane-1-sulfonic acid (DSS), the final concentration being 400 μM.

    other:

    Article Title: Mono(ADP-ribosyl)ation of 2?-deoxyguanosine residue in DNA by an apoptosis-inducing protein, pierisin-1, from cabbage butterfly
    Article Snippet: Micrococcal nuclease and phosphodiesterase I ( Crotalus adamanteus venom) and II (bovine spleen) were obtained from Worthington. β-[ Adenylate -32 P]NAD ([32 P]NAD) was purchased from NEN.

    Flow Cytometry:

    Article Title: Selective alkylation of T–T mismatched DNA using vinyldiaminotriazine–acridine conjugate
    Article Snippet: .. A solution (100 μl) of the alkylated ODN 5 or ODN 6 (100 μM), alkaline phosphatase (0.03 U/μl, Takara Bio Inc.) and phosphodiesterase I (0.03 U/μl, Worthington Biochemical Corp.) in alkaline phosphatase buffer was incubated at 37°C for 1 h. The reaction mixture was heated at 90°C for 3 min, cooled to 0°C for 3 min and analyzed by RP-HPLC (CAPCELL PAL MG-II, Shiseido, 4.6 × 250 mm, solvent A: 50 mM TEAA for analysis or HCOONH4 for purification, solvent B: MeCN, linear gradient: B 5–40%/ 30 min, flow rate: 1.0 ml/min). .. The alkylated thymidine dT* obtained by enzymatic hydrolysis of the alkylated ODN 5 and ODN 6 was dissolved in DMSO-d6 containing 10 μM 4,4-dimethyl-4-silapentane-1-sulfonic acid (DSS), the final concentration being 400 μM.

    Purification:

    Article Title: Selective alkylation of T–T mismatched DNA using vinyldiaminotriazine–acridine conjugate
    Article Snippet: .. A solution (100 μl) of the alkylated ODN 5 or ODN 6 (100 μM), alkaline phosphatase (0.03 U/μl, Takara Bio Inc.) and phosphodiesterase I (0.03 U/μl, Worthington Biochemical Corp.) in alkaline phosphatase buffer was incubated at 37°C for 1 h. The reaction mixture was heated at 90°C for 3 min, cooled to 0°C for 3 min and analyzed by RP-HPLC (CAPCELL PAL MG-II, Shiseido, 4.6 × 250 mm, solvent A: 50 mM TEAA for analysis or HCOONH4 for purification, solvent B: MeCN, linear gradient: B 5–40%/ 30 min, flow rate: 1.0 ml/min). .. The alkylated thymidine dT* obtained by enzymatic hydrolysis of the alkylated ODN 5 and ODN 6 was dissolved in DMSO-d6 containing 10 μM 4,4-dimethyl-4-silapentane-1-sulfonic acid (DSS), the final concentration being 400 μM.

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