Article Title: Detection of plasmid-mediated colistin-resistant and carbapenem-resistant genes by multiplex PCR
Figure Lengend Snippet: Agarose gel electrophoresis of PCR-amplified products from the representative four carbapenem-resistant genes and mcr-1 . Lane M = 100 bp DNA ladder, lane 1 = K. pneumoniae strain no.1385 ( bla NDM ), lane 2 = K. pneumoniae strain no. 1386 ( bla OXA-48-like ), lane 3 = E. coli strain no.A434-59 ( bla NDM and mcr-1 ), lane 4 = E. coli strain no.98 ( bla NDM and bla OXA-48-like ), lane 5 = E. coli strain no.1387 ( bla OXA-48-like ), lane 6 = K. pneumoniae strain no.1263 ( bla NDM ), lane 7 = K. pneumoniae strain 22 ( bla IMP-14a ), lane 8 = K. pneumoniae ATCC® BAA-2524 ™ ( bla OXA48 ), lane 9 = E. coli ATCC® BAA-2452 ™ ( bla NDM-1 ), lane 10 = K. pneumoniae ATCC® BAA-1705 ™ ( bla KPC ), lane 11 = E. coli AK1 (a strain carrying mcr-1 recombinant plasmid), and lane 12 = negative control (distilled water).
Article Snippet: The multiplex PCR revealed PCR product sizes of 232 bp for bla IMP-6 and bla IMP-14a in S. marcescens KU3838 and K. pneumoniae strain 22 and 34, 438 bp for bla OXA-48 in K. pneumoniae ATCC® BAA-2524™ , 621 bp for bla NDM in E. coli ATCC® BAA-2452™ , E. coli ATCC® BAA-2469™ and E. coli A434-59, 798 bp for bla KPC in K. pneumoniae ATCC® BAA-1705™ and E. coli ATCC® BAA-2340™ , and 1126 bp for mcr-1 in E. coli A434-59, respectively.
Techniques: Agarose Gel Electrophoresis, Polymerase Chain Reaction, Amplification, Recombinant, Plasmid Preparation, Negative Control