atcc baa 450 t  (ATCC)

Journal: iScience

Article Title: A coral peptide with bactericidal activity against a global marine pathogen, Vibrio coralliilyticus

doi: 10.1016/j.isci.2025.113286

Figure Lengend Snippet: Measurements of antimicrobial activity against coral-pathogenic bacteria in low-salt conditions (A–C) In 1% (w/v) NaCl medium, the antimicrobial assay was performed by exposing bacteria to 10 μM digitiferin. Antimicrobial activity was evaluated by plating all wells onto 1/10 strength ZoBell’s 2216E plates, and CFU counts after incubation at 25°C for 24 h. (D–F) In PB-mannitol medium, antimicrobial activity was determined by testing various concentrations of digitiferin. Antimicrobial activity was evaluated by plating all wells onto Marine Agar plates, and CFU counts after incubation at 30°C for 24 h. (A–F) All data are given as means ± standard error of the mean, n = 3. Statistical analysis between each sample and the negative control (0 μM digitiferin) was conducted using t test. p values less than 0.05 were considered statistically significant (ns, non-significant; p > 0.05). Significant differences are indicated by asterisks. (A and D) V. coralliilyticus P1, (B and E) V. coralliilyticus YB1, and (C and F) V. shiloi AK1.

Article Snippet: Using a liquid growth inhibition assay, antibacterial activity was assayed against Gram-negative bacteria ( E. coli [NBRC 102203 T ], P. aeruginosa [NBRC 12689 T ], and S. marcescens [ATCC BAA-632]), Gram-positive bacteria ( B. subtilis [NBRC 13719 T ] and S. aureus [NBRC 100910 T ]) and coral-pathogenic bacteria ( V. coralliilyticus P1 [LMG23696], V. coralliilyticus YB1 [ATCC BAA-450 T ], V. shiloi AK1 [ATCC BAA-91 T ] and S . marcescens [ATCC BAA-632]).

Techniques: Activity Assay, Bacteria, Incubation, Negative Control

Journal: iScience

Article Title: A coral peptide with bactericidal activity against a global marine pathogen, Vibrio coralliilyticus

doi: 10.1016/j.isci.2025.113286

Figure Lengend Snippet: Measurements of antimicrobial activity against coral-pathogenic bacteria in low-salt conditions (A–C) In 1% (w/v) NaCl medium, the antimicrobial assay was performed by exposing bacteria to 10 μM digitiferin. Antimicrobial activity was evaluated by plating all wells onto 1/10 strength ZoBell’s 2216E plates, and CFU counts after incubation at 25°C for 24 h. (D–F) In PB-mannitol medium, antimicrobial activity was determined by testing various concentrations of digitiferin. Antimicrobial activity was evaluated by plating all wells onto Marine Agar plates, and CFU counts after incubation at 30°C for 24 h. (A–F) All data are given as means ± standard error of the mean, n = 3. Statistical analysis between each sample and the negative control (0 μM digitiferin) was conducted using t test. p values less than 0.05 were considered statistically significant (ns, non-significant; p > 0.05). Significant differences are indicated by asterisks. (A and D) V. coralliilyticus P1, (B and E) V. coralliilyticus YB1, and (C and F) V. shiloi AK1.

Article Snippet: Vibrio coralliilyticus YB1 , ATCC , ATCC BAA-450 T.

Techniques: Activity Assay, Bacteria, Incubation, Negative Control