hca vsmc cc 2583 cells  (Lonza)


Bioz Manufacturer Symbol Lonza manufactures this product  
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    Lonza hca vsmc cc 2583 cells
    Hca Vsmc Cc 2583 Cells, supplied by Lonza, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hca vsmc cc 2583 cells  (Lonza)


    Bioz Manufacturer Symbol Lonza manufactures this product  
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    Lonza hca vsmc cc 2583 cells
    Hca Vsmc Cc 2583 Cells, supplied by Lonza, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hca vsmc cc 2583 cells/product/Lonza
    Average 86 stars, based on 1 article reviews
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    hca vsmc cc 2583 cells - by Bioz Stars, 2024-09
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    human coronary artery smooth muscle cells  (Lonza)


    Bioz Manufacturer Symbol Lonza manufactures this product  
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    Lonza human coronary artery smooth muscle cells
    a, Immunoblotting images showing the protein levels of HIF1α and its targets in 9 types of normal and cancer <t>cells</t> under normoxia (N) and hypoxia (H; 0.2% O 2 for 24 hours). n = 5-8. b, Confocal microscopy shows HIF1α expression in aerobic cell cultures of <t>human</t> PASMCs, PAECs, and AoECs. n = 3. c, HIF1α and <t>α-smooth</t> <t>muscle</t> actin (αSMA, a marker of VSMCs) expression in human lung tissues of transplantation-failed donors. n = 3. d, Glycolytic extracellular acidification rate (ECAR) in normoxic cell cultures determined by a Seahorse glycolytic stress assay. n = 5-9. e,f, PFKFB3 ( e ) and LDHA ( f ) protein levels in cells cultured in 21% or 0.2% O 2 for 24 hours. Fold change was calculated relative to corresponding normoxic cultures of each cell type. n = 5-8. g, Extracellular lactate levels in different normal and cancer cell types under normoxia or hypoxia (0.2% O 2 ) for 24 hours. n = 4-5. h, mRNA expression of HIF1α and its known transcriptional genes in aerobic cultures of PASMCs transfected with control siRNA (siCtrl) or HIF1α siRNA (siHIF1α). n = 9. i,j, Seahorse assays show ECAR ( i ) and oxygen consumption rate (OCR; j ) in PASMCs with HIF1α knockdown under normoxia. n = 3. k, Lactate secretion by PASMCs with HIF1α knockdown. Fold change was calculated relative to siCtrl-transfected cells. n = 7. All data were presented as mean ± SD. One-way ANOVA followed by Dunnett’s post-hoc test ( d ), Student’s t test or Mann-Whitney U test ( e-k ) was used when compared to aerobic culture of PASMCs or the matched cell type ( d-g ), or siCtrl-transfected PASMCs ( h-k ).
    Human Coronary Artery Smooth Muscle Cells, supplied by Lonza, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
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    human coronary artery smooth muscle cells - by Bioz Stars, 2024-09
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    1) Product Images from "Branched chain α-ketoacids aerobically activate HIF1α signaling in vascular cells"

    Article Title: Branched chain α-ketoacids aerobically activate HIF1α signaling in vascular cells

    Journal: bioRxiv

    doi: 10.1101/2024.05.29.595538

    a, Immunoblotting images showing the protein levels of HIF1α and its targets in 9 types of normal and cancer cells under normoxia (N) and hypoxia (H; 0.2% O 2 for 24 hours). n = 5-8. b, Confocal microscopy shows HIF1α expression in aerobic cell cultures of human PASMCs, PAECs, and AoECs. n = 3. c, HIF1α and α-smooth muscle actin (αSMA, a marker of VSMCs) expression in human lung tissues of transplantation-failed donors. n = 3. d, Glycolytic extracellular acidification rate (ECAR) in normoxic cell cultures determined by a Seahorse glycolytic stress assay. n = 5-9. e,f, PFKFB3 ( e ) and LDHA ( f ) protein levels in cells cultured in 21% or 0.2% O 2 for 24 hours. Fold change was calculated relative to corresponding normoxic cultures of each cell type. n = 5-8. g, Extracellular lactate levels in different normal and cancer cell types under normoxia or hypoxia (0.2% O 2 ) for 24 hours. n = 4-5. h, mRNA expression of HIF1α and its known transcriptional genes in aerobic cultures of PASMCs transfected with control siRNA (siCtrl) or HIF1α siRNA (siHIF1α). n = 9. i,j, Seahorse assays show ECAR ( i ) and oxygen consumption rate (OCR; j ) in PASMCs with HIF1α knockdown under normoxia. n = 3. k, Lactate secretion by PASMCs with HIF1α knockdown. Fold change was calculated relative to siCtrl-transfected cells. n = 7. All data were presented as mean ± SD. One-way ANOVA followed by Dunnett’s post-hoc test ( d ), Student’s t test or Mann-Whitney U test ( e-k ) was used when compared to aerobic culture of PASMCs or the matched cell type ( d-g ), or siCtrl-transfected PASMCs ( h-k ).
    Figure Legend Snippet: a, Immunoblotting images showing the protein levels of HIF1α and its targets in 9 types of normal and cancer cells under normoxia (N) and hypoxia (H; 0.2% O 2 for 24 hours). n = 5-8. b, Confocal microscopy shows HIF1α expression in aerobic cell cultures of human PASMCs, PAECs, and AoECs. n = 3. c, HIF1α and α-smooth muscle actin (αSMA, a marker of VSMCs) expression in human lung tissues of transplantation-failed donors. n = 3. d, Glycolytic extracellular acidification rate (ECAR) in normoxic cell cultures determined by a Seahorse glycolytic stress assay. n = 5-9. e,f, PFKFB3 ( e ) and LDHA ( f ) protein levels in cells cultured in 21% or 0.2% O 2 for 24 hours. Fold change was calculated relative to corresponding normoxic cultures of each cell type. n = 5-8. g, Extracellular lactate levels in different normal and cancer cell types under normoxia or hypoxia (0.2% O 2 ) for 24 hours. n = 4-5. h, mRNA expression of HIF1α and its known transcriptional genes in aerobic cultures of PASMCs transfected with control siRNA (siCtrl) or HIF1α siRNA (siHIF1α). n = 9. i,j, Seahorse assays show ECAR ( i ) and oxygen consumption rate (OCR; j ) in PASMCs with HIF1α knockdown under normoxia. n = 3. k, Lactate secretion by PASMCs with HIF1α knockdown. Fold change was calculated relative to siCtrl-transfected cells. n = 7. All data were presented as mean ± SD. One-way ANOVA followed by Dunnett’s post-hoc test ( d ), Student’s t test or Mann-Whitney U test ( e-k ) was used when compared to aerobic culture of PASMCs or the matched cell type ( d-g ), or siCtrl-transfected PASMCs ( h-k ).

    Techniques Used: Western Blot, Confocal Microscopy, Expressing, Marker, Transplantation Assay, Cell Culture, Transfection, MANN-WHITNEY

    technology nist  (National Institute of Standards and Technology)

     
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    National Institute of Standards and Technology technology nist
    Technology Nist, supplied by National Institute of Standards and Technology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/technology nist/product/National Institute of Standards and Technology
    Average 86 stars, based on 1 article reviews
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    technology nist - by Bioz Stars, 2024-09
    86/100 stars

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    technology nist  (National Institute of Standards and Technology)

     
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    National Institute of Standards and Technology technology nist
    Experimental approach conducted within this study. An exposure to the A549 + d-THP-1 co-culture of indoor air pollution particles <t>(NIST</t> <t>2583)</t> is completed and analysed 24 h after exposure via the VitroCell (aerosol) exposure method. Created with BioRender.com
    Technology Nist, supplied by National Institute of Standards and Technology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    technology nist - by Bioz Stars, 2024-09
    86/100 stars

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    1) Product Images from "Determining the toxicological effects of indoor air pollution on both a healthy and an inflammatory-comprised model of the alveolar epithelial barrier in vitro"

    Article Title: Determining the toxicological effects of indoor air pollution on both a healthy and an inflammatory-comprised model of the alveolar epithelial barrier in vitro

    Journal: Particle and Fibre Toxicology

    doi: 10.1186/s12989-024-00584-8

    Experimental approach conducted within this study. An exposure to the A549 + d-THP-1 co-culture of indoor air pollution particles (NIST 2583) is completed and analysed 24 h after exposure via the VitroCell (aerosol) exposure method. Created with BioRender.com
    Figure Legend Snippet: Experimental approach conducted within this study. An exposure to the A549 + d-THP-1 co-culture of indoor air pollution particles (NIST 2583) is completed and analysed 24 h after exposure via the VitroCell (aerosol) exposure method. Created with BioRender.com

    Techniques Used: Co-Culture Assay, Aerosol

    SEM image and EDX analysis of NIST 2583 suspended in distilled water. Scale bar is 100µm ( A ) and 50µm ( B ) with the EDX analysis completed at the site indicated within ( B )
    Figure Legend Snippet: SEM image and EDX analysis of NIST 2583 suspended in distilled water. Scale bar is 100µm ( A ) and 50µm ( B ) with the EDX analysis completed at the site indicated within ( B )

    Techniques Used:

    Viability and membrane integrity, 24 h post exposure of NIST 2583 on the unstimulated A549 + dTHP-1 co-culture. Cells were exposed for 24 h at an ALI using the VitroCell Cloud12, before analysing cytotoxicity ( A ) and membrane integrity (blue dextran) ( B ). n = 3 with all assays performed in triplicate. The data is presented as the mean ± standard deviation. Significance is denoted as the following: compared to the medium control p < 0.01(*)
    Figure Legend Snippet: Viability and membrane integrity, 24 h post exposure of NIST 2583 on the unstimulated A549 + dTHP-1 co-culture. Cells were exposed for 24 h at an ALI using the VitroCell Cloud12, before analysing cytotoxicity ( A ) and membrane integrity (blue dextran) ( B ). n = 3 with all assays performed in triplicate. The data is presented as the mean ± standard deviation. Significance is denoted as the following: compared to the medium control p < 0.01(*)

    Techniques Used: Membrane, Co-Culture Assay, Standard Deviation

    Cell morphology 24 h post exposure of NIST 2583 on the unstimulated A549 + dTHP-1 co-culture. Cells were exposed for 24 h at an ALI using the VitroCell Cloud12—Negative control ( A ), 232ng/cm 2 ( B ), 464ng/cm 2 ( C ), and 608ng/cm 2 ( D ). DAPI is indicated by blue while phalloidin is indicated by red. Scale bar is 50µm
    Figure Legend Snippet: Cell morphology 24 h post exposure of NIST 2583 on the unstimulated A549 + dTHP-1 co-culture. Cells were exposed for 24 h at an ALI using the VitroCell Cloud12—Negative control ( A ), 232ng/cm 2 ( B ), 464ng/cm 2 ( C ), and 608ng/cm 2 ( D ). DAPI is indicated by blue while phalloidin is indicated by red. Scale bar is 50µm

    Techniques Used: Co-Culture Assay, Negative Control

    Genotoxicology assessment 24 h post exposure of NIST 2583 on the unstimulated A549 + dTHP-1 co-culture. Cells were exposed for 24 h at an ALI using the VitroCell Cloud12, before completing the comet assay ( A ) and CBMN assay ( B ). n = 3 with all assays performed in triplicate. The data is presented as the mean ± standard deviation. Significance is denoted as the following: compared to the negative control p < 0.01(*)
    Figure Legend Snippet: Genotoxicology assessment 24 h post exposure of NIST 2583 on the unstimulated A549 + dTHP-1 co-culture. Cells were exposed for 24 h at an ALI using the VitroCell Cloud12, before completing the comet assay ( A ) and CBMN assay ( B ). n = 3 with all assays performed in triplicate. The data is presented as the mean ± standard deviation. Significance is denoted as the following: compared to the negative control p < 0.01(*)

    Techniques Used: Co-Culture Assay, Single Cell Gel Electrophoresis, Standard Deviation, Negative Control

    Pro-inflammatory mediators of interest 24 h post exposure of NIST 2583 on the unstimulated A549 + dTHP-1 co-culture. TNF-α ( A ), IL-8 ( B ), IL-33 ( C ), SLPI ( D ), IL-6 ( E ) and IL-13 ( F ) basal concentration 24 h post exposure after the particle exposure (onto the apical side). Cells were exposed for 24 h at an ALI using the VitroCell Cloud12 and left to incubate for 24 h. n = 3 with all assays performed in triplicate. The data is presented as the mean ± standard deviation. Significance is denoted as the following: compared to the medium control p < 0.01(*). N.D – non-detection
    Figure Legend Snippet: Pro-inflammatory mediators of interest 24 h post exposure of NIST 2583 on the unstimulated A549 + dTHP-1 co-culture. TNF-α ( A ), IL-8 ( B ), IL-33 ( C ), SLPI ( D ), IL-6 ( E ) and IL-13 ( F ) basal concentration 24 h post exposure after the particle exposure (onto the apical side). Cells were exposed for 24 h at an ALI using the VitroCell Cloud12 and left to incubate for 24 h. n = 3 with all assays performed in triplicate. The data is presented as the mean ± standard deviation. Significance is denoted as the following: compared to the medium control p < 0.01(*). N.D – non-detection

    Techniques Used: Co-Culture Assay, Concentration Assay, Standard Deviation

    Viability and membrane integrity, 24 h post exposure of NIST 2583 on an “inflamed” A549 + dTHP-1 co-culture. Cells were exposed for 24 h at an ALI using the VitroCell Cloud12, before analysing cytotoxicity ( A ) and membrane integrity (blue dextran) ( B ). n = 3 with all assays performed in triplicate. The data is presented as the mean ± standard deviation. Significance is denoted as the following: compared to the medium control p < 0.01(*)
    Figure Legend Snippet: Viability and membrane integrity, 24 h post exposure of NIST 2583 on an “inflamed” A549 + dTHP-1 co-culture. Cells were exposed for 24 h at an ALI using the VitroCell Cloud12, before analysing cytotoxicity ( A ) and membrane integrity (blue dextran) ( B ). n = 3 with all assays performed in triplicate. The data is presented as the mean ± standard deviation. Significance is denoted as the following: compared to the medium control p < 0.01(*)

    Techniques Used: Membrane, Co-Culture Assay, Standard Deviation

    Cell morphology 24 h post exposure of NIST 2583 on an “inflamed” A549 + dTHP-1 co-culture. Cells were exposed for 24 h at an ALI using the VitroCell Cloud12—Negative control ( A ), 232ng/cm 2 ( B ), 464ng/cm 2 ( C ), and 608ng/cm 2 ( D ). DAPI is indicated by blue while phalloidin is indicated by red. Scale bar is 50µm
    Figure Legend Snippet: Cell morphology 24 h post exposure of NIST 2583 on an “inflamed” A549 + dTHP-1 co-culture. Cells were exposed for 24 h at an ALI using the VitroCell Cloud12—Negative control ( A ), 232ng/cm 2 ( B ), 464ng/cm 2 ( C ), and 608ng/cm 2 ( D ). DAPI is indicated by blue while phalloidin is indicated by red. Scale bar is 50µm

    Techniques Used: Co-Culture Assay, Negative Control

    Genotoxicology assessment 24 h post exposure of NIST 2583 on an “inflamed” A549 + dTHP-1 co-culture. Cells were exposed for 24 h at an ALI using the VitroCell Cloud12, before completing the comet assay ( A ) and CBMN assay ( B ). n = 3 with all assays performed in triplicate. The data is presented as the mean ± standard deviation. Significance is denoted as the following: compared to the negative control p < 0.01(*)
    Figure Legend Snippet: Genotoxicology assessment 24 h post exposure of NIST 2583 on an “inflamed” A549 + dTHP-1 co-culture. Cells were exposed for 24 h at an ALI using the VitroCell Cloud12, before completing the comet assay ( A ) and CBMN assay ( B ). n = 3 with all assays performed in triplicate. The data is presented as the mean ± standard deviation. Significance is denoted as the following: compared to the negative control p < 0.01(*)

    Techniques Used: Co-Culture Assay, Single Cell Gel Electrophoresis, Standard Deviation, Negative Control

    Inflammatory mediators of interest 24 h post exposure of NIST 2583 on an “inflamed” A549 + dTHP-1 co-culture. TNF-α ( A ), IL-1β ( B ), IL-33 ( C ), SLPI ( D ), IL-6 ( E ) and IL-10 ( F ) basal concentration was analysed 24 h post particle exposure (onto the apical side). Cells were exposed for 24 h at an ALI using the VitroCell Cloud12 and left to incubate for 24 h. n = 3 with all assays performed in triplicate. The data is presented as the mean ± standard deviation. Significance is denoted as the following: compared to the medium control p < 0.01(*)
    Figure Legend Snippet: Inflammatory mediators of interest 24 h post exposure of NIST 2583 on an “inflamed” A549 + dTHP-1 co-culture. TNF-α ( A ), IL-1β ( B ), IL-33 ( C ), SLPI ( D ), IL-6 ( E ) and IL-10 ( F ) basal concentration was analysed 24 h post particle exposure (onto the apical side). Cells were exposed for 24 h at an ALI using the VitroCell Cloud12 and left to incubate for 24 h. n = 3 with all assays performed in triplicate. The data is presented as the mean ± standard deviation. Significance is denoted as the following: compared to the medium control p < 0.01(*)

    Techniques Used: Co-Culture Assay, Concentration Assay, Standard Deviation

    nist srm 2583 indoor dust standard  (Merck & Co)

     
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    Merck & Co nist srm 2583 indoor dust standard
    Nist Srm 2583 Indoor Dust Standard, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nist srm 2583 indoor dust standard/product/Merck & Co
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    nist srm 2583 indoor dust standard - by Bioz Stars, 2024-09
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    ia 50063  (Hy-Line North America LLC)

     
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    Hy-Line North America LLC ia 50063
    Ia 50063, supplied by Hy-Line North America LLC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ia 50063/product/Hy-Line North America LLC
    Average 86 stars, based on 1 article reviews
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    ia 50063 - by Bioz Stars, 2024-09
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    coronary artery smooth muscle cells  (Lonza)


    Bioz Manufacturer Symbol Lonza manufactures this product  
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    Lonza coronary artery smooth muscle cells
    Coronary Artery Smooth Muscle Cells, supplied by Lonza, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/coronary artery smooth muscle cells/product/Lonza
    Average 86 stars, based on 1 article reviews
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    dust standard reference material  (National Institute of Standards and Technology)

     
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    National Institute of Standards and Technology dust standard reference material
    Dust Standard Reference Material, supplied by National Institute of Standards and Technology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dust standard reference material/product/National Institute of Standards and Technology
    Average 86 stars, based on 1 article reviews
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    scanning microscopy  (Nikon)

     
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    Nikon scanning microscopy
    Scanning Microscopy, supplied by Nikon, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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