Journal: Biotechnology and bioengineering

Article Title: Bioinformatics-Driven Discovery of Novel Clostridioides difficile Lysins and Experimental Comparison with Highly Active Benchmarks

doi: 10.1002/bit.27759

Figure Lengend Snippet: Specific lytic rates of CATs towards panel of C. difficile isolates. Strains are (A) BAA-1801, (B) BAA-1382, (C) BAA-1814, and (D) BAA-1812. (E) Average normalized activity across the entire panel of 10 C. difficile isolates, where the remaining isolates are provided in Fig. S2. Shown are mean and standard deviation of assay replicates (n=5).

Article Snippet: Preparation of C. difficile Spores Single colonies of C. difficile strain ATCC BAA-1801, chosen for its consistent results when evaluating colony forming units from spores under our experimental conditions, were inoculated into cooked meat broth and incubated anaerobically at 37°C.

Techniques: Activity Assay, Standard Deviation

Journal: Biotechnology and bioengineering

Article Title: Bioinformatics-Driven Discovery of Novel Clostridioides difficile Lysins and Experimental Comparison with Highly Active Benchmarks

doi: 10.1002/bit.27759

Figure Lengend Snippet: Bactericidal activity of CATs towards actively germinating spores. CFUs from quantitative cultures following germination of C. difficile strain BAA-1801 spores and CAT treatment. Shown are mean and standard deviation of assay replicates (n=4).

Article Snippet: Preparation of C. difficile Spores Single colonies of C. difficile strain ATCC BAA-1801, chosen for its consistent results when evaluating colony forming units from spores under our experimental conditions, were inoculated into cooked meat broth and incubated anaerobically at 37°C.

Techniques: Activity Assay, Standard Deviation

Journal: Biotechnology and bioengineering

Article Title: Bioinformatics-Driven Discovery of Novel Clostridioides difficile Lysins and Experimental Comparison with Highly Active Benchmarks

doi: 10.1002/bit.27759

Figure Lengend Snippet: Specific lytic rates of CATs towards panel of C. difficile isolates. Strains are (A) BAA-1801, (B) BAA-1382, (C) BAA-1814, and (D) BAA-1812. (E) Average normalized activity across the entire panel of 10 C. difficile isolates, where the remaining isolates are provided in Fig. S2. Shown are mean and standard deviation of assay replicates (n=5).

Article Snippet: Bacterial Strains and Culturing Conditions C. difficile strains ATCC BAA-1804, ATCC BAA-1870, ATCC BAA-1803, ATCC BAA-1801, ATCC BAA-1875, ATCC BAA-1812, ATCC BAA-1814, ATCC 43598, and ATCC BAA-1382 were obtained from ATCC while C. difficile strain ATCC 9689 was acquired from Microbiologics.

Techniques: Activity Assay, Standard Deviation

Journal: Biotechnology and bioengineering

Article Title: Bioinformatics-Driven Discovery of Novel Clostridioides difficile Lysins and Experimental Comparison with Highly Active Benchmarks

doi: 10.1002/bit.27759

Figure Lengend Snippet: Bactericidal activity of CATs towards actively germinating spores. CFUs from quantitative cultures following germination of C. difficile strain BAA-1801 spores and CAT treatment. Shown are mean and standard deviation of assay replicates (n=4).

Article Snippet: Bacterial Strains and Culturing Conditions C. difficile strains ATCC BAA-1804, ATCC BAA-1870, ATCC BAA-1803, ATCC BAA-1801, ATCC BAA-1875, ATCC BAA-1812, ATCC BAA-1814, ATCC 43598, and ATCC BAA-1382 were obtained from ATCC while C. difficile strain ATCC 9689 was acquired from Microbiologics.

Techniques: Activity Assay, Standard Deviation