Nmuli, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Francisellatularensis Uses Cholesterol and Clathrin-Based Endocytic Mechanisms to Invade Hepatocytes"
Article Title: Francisellatularensis Uses Cholesterol and Clathrin-Based Endocytic Mechanisms to Invade Hepatocytes
Journal: Scientific Reports
Figure Legend Snippet: F. novicida invades and replicates within BNL CL.2 and NMuLi hepatocytes. (A) Immunofluorescent images of F. novicida infections in cultured hepatocytes. Hepatocytes were fixed following 24 h F. novicida infections with both BNL CL.2 and NMuLi cells. Samples were labelled with anti- F. novicida antibodies (green), fluorescent phalloidin to indicate the cell boundaries (red) and DAPI (blue). Arrowheads indicate some of the bacteria within the infected cells. Scale bar = 10m. (B) Quantification of the proportion of infected hepatocytes as assessed by microscopic examination. NMuLi (n = 17) and BNL CL.2 (n = 16). (C) Titre of intracellular F. novicida at various timepoints following infection of BNL CL.2 and NMuLi cell infections by invasion assay (n = 3).
Techniques Used: Cell Culture, Infection, Invasion Assay
Figure Legend Snippet: F. novicida entry does not utilize caveolin-dependent or macropinocytosis pathways. (A) BNL CL.2 and NMuLi cells were treated with the caveolin-inhibitor filipin and infected with F. novicida for 22 h followed by 2 h gentamicin treatment for invasion assays. (B) Immunolocalization of caveolin-1 (green) and DAPI (blue) of uninfected BNL CL.2 cells or following 8 h, 16 h, and 24 h infections with F. novicida showed no co-localization between F. novicida and caveolin-1. Arrowheads indicate the localization of F. novicida . Scale bar = 5m. (C) 5mM of amiloride was used to pre-treat BNL CL.2 cells and HeLa cells for 15 min prior to infection with F. novicida and Salmonella Thyphimurium (SL1344), respectively. Untreated cells were treated with media containing DMSO. Cells infected with F. novicida for 6 h followed by 2 h gentamicin treatment did not show a decrease in invasion while cells infected with S. Typhimurium for 30 min followed by 1 h gentamicin treatment showed a significant decrease in invasion *P
Techniques Used: Infection