Journal: Standards in Genomic Sciences
Article Title: Draft genome sequence of Mycobacterium rufum JS14 T , a polycyclic-aromatic-hydrocarbon-degrading bacterium from petroleum-contaminated soil in Hawaii
doi: 10.1186/s40793-016-0167-5
Figure Lengend Snippet: A neighbor-joining phylogenetic tree depicting the position of M. rufum JS14 T (shown in boldface with an asterisk) relative to the other species within the genus Mycobacterium . In this genus, species carrying the full length of 16S rRNA gene sequence were selected from the NCBI database . The collected nucleotide sequences were aligned using ClustalW , and the phylogenetic tree was constructed using software MEGA version 6 by the neighbor-joining method with 1000 bootstrap replicates . The generated bootstrap values for each species are presented at the nodes, and the scale bar indicates 0.005 nucleotide changes per nucleotide position. The strains under study and their corresponding GenBank accession numbers for 16S rRNA genes are as follows: M. chlorophenolicum PCP-I [ , ] (NR_119093); M. gilvum Spyr1 [ , ] (NR_074644); M. gilvum PYR-GCK [ , ] (NR_074553); M. rhodesiae NBB3 (NR_102870); M. vanbaalenii PYR-1 (NR_074572); M. fluoranthenivorans FA4 [ , ] (NR_042224); M. wolinskyi 700010 (NR_119253); M. mageritense 938 (NR_042265); M. smegmatis str. MC2 155 [ , ] (NR_074726); M. flavescens ATCC 14474 [ , ] (NR_044815); M. novocastrense 73 (NR_029208); M. insubricum FI-06250 (NR_125525); M. florentinum FI-93171 (NR_042223); M. montefiorense ATCC BAA-256 [ , ] (NR_028808); M. confluentis 1389/90 (NR_042245); M. holsaticum 1406 (NR_028945); M. elephantis DSM 44368 (NR_025296); M. marinum M [ , ] (NR_074864); M. ulcerans Agy99 [ , ] (NR_074861); M. bovis BCG str. Pasteur 1173P2 [ , ] (NR_074838); M. canettii CIPT 140010059 (NR_074836); M. africanum GM041182 [ , ] (NR_074835)
Article Snippet: Mycobacterium rufum JS14 T (=ATCC BAA-1377 T , CIP 109273 T , JCM 16372 T , DSM 45406 T ), a type strain of the species Mycobacterium rufum sp. . belonging to the family Mycobacteriaceae , was isolated from polycyclic aromatic hydrocarbon (PAH)-contaminated soil in Hilo (HI, USA) because it harbors the capability of degrading PAH.
Techniques: Sequencing, Construct, Software, Generated