d556  (ATCC)


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    ATCC d556
    D556, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/d556/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    d556 - by Bioz Stars, 2024-06
    86/100 stars

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    d556  (ATCC)


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    ATCC d556
    LIN28B promotes Group 3 medulloblastoma viability and proliferation. (A) Relative levels of LIN28B protein in Group 3 MB cell lines <t>D556,</t> HDMB03, D341, and D425 and patient‐derived cells BT52 and BT52CTC. (B) CellTiter‐Glo assay demonstrates a significant reduction in viability in shLIN28B cells compared with shctl cells. LIN28B levels in HDMB03, D341, and D425 cells treated with either control shRNA (shctl) or LIN28B shRNA (shLIN28B‐1) demonstrate reduction in LIN28B following shRNA transfection (see Fig. for experiments using a 2 nd shRNA (shLIN28B‐5)). (C) BrdU incorporation analysis using immunofluorescence in Group 3 MB cell lines demonstrates a significant decrease in BrdU‐positive cells following LIN28B knockdown ( n = 3 for each group). (D) In D341 and (E), HDMB03 shLIN28B‐1 knockdown cells LIN28B rescue leads to restoration of cell viability. Western blot of corresponding cell lysates demonstrating rescue of LIN28B protein in knockdown cells (LV105 overexpression plasmid control; LVLIN28B LIN28B expression plasmid) is shown below (unpaired t ‐test P ‐value: * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; n = 3 for each group). Error bars represent SEM.
    D556, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/d556/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    d556 - by Bioz Stars, 2024-06
    86/100 stars

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    1) Product Images from "The LIN28B –let‐7– PBK pathway is essential for group 3 medulloblastoma tumor growth and survival"

    Article Title: The LIN28B –let‐7– PBK pathway is essential for group 3 medulloblastoma tumor growth and survival

    Journal: Molecular Oncology

    doi: 10.1002/1878-0261.13477

    LIN28B promotes Group 3 medulloblastoma viability and proliferation. (A) Relative levels of LIN28B protein in Group 3 MB cell lines D556, HDMB03, D341, and D425 and patient‐derived cells BT52 and BT52CTC. (B) CellTiter‐Glo assay demonstrates a significant reduction in viability in shLIN28B cells compared with shctl cells. LIN28B levels in HDMB03, D341, and D425 cells treated with either control shRNA (shctl) or LIN28B shRNA (shLIN28B‐1) demonstrate reduction in LIN28B following shRNA transfection (see Fig. for experiments using a 2 nd shRNA (shLIN28B‐5)). (C) BrdU incorporation analysis using immunofluorescence in Group 3 MB cell lines demonstrates a significant decrease in BrdU‐positive cells following LIN28B knockdown ( n = 3 for each group). (D) In D341 and (E), HDMB03 shLIN28B‐1 knockdown cells LIN28B rescue leads to restoration of cell viability. Western blot of corresponding cell lysates demonstrating rescue of LIN28B protein in knockdown cells (LV105 overexpression plasmid control; LVLIN28B LIN28B expression plasmid) is shown below (unpaired t ‐test P ‐value: * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; n = 3 for each group). Error bars represent SEM.
    Figure Legend Snippet: LIN28B promotes Group 3 medulloblastoma viability and proliferation. (A) Relative levels of LIN28B protein in Group 3 MB cell lines D556, HDMB03, D341, and D425 and patient‐derived cells BT52 and BT52CTC. (B) CellTiter‐Glo assay demonstrates a significant reduction in viability in shLIN28B cells compared with shctl cells. LIN28B levels in HDMB03, D341, and D425 cells treated with either control shRNA (shctl) or LIN28B shRNA (shLIN28B‐1) demonstrate reduction in LIN28B following shRNA transfection (see Fig. for experiments using a 2 nd shRNA (shLIN28B‐5)). (C) BrdU incorporation analysis using immunofluorescence in Group 3 MB cell lines demonstrates a significant decrease in BrdU‐positive cells following LIN28B knockdown ( n = 3 for each group). (D) In D341 and (E), HDMB03 shLIN28B‐1 knockdown cells LIN28B rescue leads to restoration of cell viability. Western blot of corresponding cell lysates demonstrating rescue of LIN28B protein in knockdown cells (LV105 overexpression plasmid control; LVLIN28B LIN28B expression plasmid) is shown below (unpaired t ‐test P ‐value: * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; n = 3 for each group). Error bars represent SEM.

    Techniques Used: Derivative Assay, Glo Assay, shRNA, Transfection, BrdU Incorporation Assay, Immunofluorescence, Western Blot, Over Expression, Plasmid Preparation, Expressing

    d556  (ATCC)


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    ATCC d556
    D556, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/d556/product/ATCC
    Average 86 stars, based on 1 article reviews
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    aust  (ATCC)


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    ATCC aust
    Aust, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    aust - by Bioz Stars, 2024-06
    86/100 stars

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