dnase i  (Worthington Biochemical)


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  • 99
    Name:
    Deoxyribonuclease I
    Description:
    Chromatographically purified A lyophilized powder with glycine as a stabilizer
    Catalog Number:
    ls002004
    Price:
    33
    Size:
    5 mg
    Source:
    Bovine Pancreas
    Cas Number:
    9003.98.9
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    Structured Review

    Worthington Biochemical dnase i
    Constitutive binding to interphase chromatin of an NFAT5a mutant lacking the CTD. (A) Flow cytometry analysis of the cell cycle profile ( left histogram ) and proportion of interphase and mitotic cells ( dot plot ) in asynchronous HEK293 cultures. (B) HEK293 cells cultured in isotonic medium (310 mOsm/kg) or exposed to hypertonic conditions (470 mOsm/kg) during 4 hours were lysed and proteins were fractionated into soluble (S) or chromatin (Ch) fractions. One set of samples was treated with <t>DNase</t> I during lysis, which caused the release of chromatin-associated proteins to the soluble fraction. NFAT5 and markers of soluble (pyruvate kinase) and chromatin-associated proteins (histone H3) were detected by Western blotting. (C) HEK293 cells expressing Myc-tagged full length NFAT5a (FL5), a DNA-binding mutant (FL5 DB1 ), or a construct comprising the amino-terminal region plus DNA-binding domain (ND5) (diagram in Figure 1B ) and its DNA-binding mutant (ND5 DB1 ) were cultured in isotonic medium (290 mOsm/kg) or exposed to hypertonic conditions (470 mOsm/kg) during 4 hours, lysed, and proteins were fractionated into soluble (S) or chromatin (Ch) fractions. NFAT5 constructs were detected by Western blotting with an anti-Myc antibody. Results shown are representative of four independent experiments.
    Chromatographically purified A lyophilized powder with glycine as a stabilizer
    https://www.bioz.com/result/dnase i/product/Worthington Biochemical
    Average 99 stars, based on 934 article reviews
    Price from $9.99 to $1999.99
    dnase i - by Bioz Stars, 2020-05
    99/100 stars

    Images

    1) Product Images from "Exclusion of NFAT5 from Mitotic Chromatin Resets Its Nucleo-Cytoplasmic Distribution in Interphase"

    Article Title: Exclusion of NFAT5 from Mitotic Chromatin Resets Its Nucleo-Cytoplasmic Distribution in Interphase

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0007036

    Constitutive binding to interphase chromatin of an NFAT5a mutant lacking the CTD. (A) Flow cytometry analysis of the cell cycle profile ( left histogram ) and proportion of interphase and mitotic cells ( dot plot ) in asynchronous HEK293 cultures. (B) HEK293 cells cultured in isotonic medium (310 mOsm/kg) or exposed to hypertonic conditions (470 mOsm/kg) during 4 hours were lysed and proteins were fractionated into soluble (S) or chromatin (Ch) fractions. One set of samples was treated with DNase I during lysis, which caused the release of chromatin-associated proteins to the soluble fraction. NFAT5 and markers of soluble (pyruvate kinase) and chromatin-associated proteins (histone H3) were detected by Western blotting. (C) HEK293 cells expressing Myc-tagged full length NFAT5a (FL5), a DNA-binding mutant (FL5 DB1 ), or a construct comprising the amino-terminal region plus DNA-binding domain (ND5) (diagram in Figure 1B ) and its DNA-binding mutant (ND5 DB1 ) were cultured in isotonic medium (290 mOsm/kg) or exposed to hypertonic conditions (470 mOsm/kg) during 4 hours, lysed, and proteins were fractionated into soluble (S) or chromatin (Ch) fractions. NFAT5 constructs were detected by Western blotting with an anti-Myc antibody. Results shown are representative of four independent experiments.
    Figure Legend Snippet: Constitutive binding to interphase chromatin of an NFAT5a mutant lacking the CTD. (A) Flow cytometry analysis of the cell cycle profile ( left histogram ) and proportion of interphase and mitotic cells ( dot plot ) in asynchronous HEK293 cultures. (B) HEK293 cells cultured in isotonic medium (310 mOsm/kg) or exposed to hypertonic conditions (470 mOsm/kg) during 4 hours were lysed and proteins were fractionated into soluble (S) or chromatin (Ch) fractions. One set of samples was treated with DNase I during lysis, which caused the release of chromatin-associated proteins to the soluble fraction. NFAT5 and markers of soluble (pyruvate kinase) and chromatin-associated proteins (histone H3) were detected by Western blotting. (C) HEK293 cells expressing Myc-tagged full length NFAT5a (FL5), a DNA-binding mutant (FL5 DB1 ), or a construct comprising the amino-terminal region plus DNA-binding domain (ND5) (diagram in Figure 1B ) and its DNA-binding mutant (ND5 DB1 ) were cultured in isotonic medium (290 mOsm/kg) or exposed to hypertonic conditions (470 mOsm/kg) during 4 hours, lysed, and proteins were fractionated into soluble (S) or chromatin (Ch) fractions. NFAT5 constructs were detected by Western blotting with an anti-Myc antibody. Results shown are representative of four independent experiments.

    Techniques Used: Binding Assay, Mutagenesis, Flow Cytometry, Cytometry, Cell Culture, Lysis, Western Blot, Expressing, Construct

    Related Articles

    In Vitro:

    Article Title: Extracellular traps are associated with human and mouse neutrophil and macrophage mediated killing of larval Strongyloides stercoralis
    Article Snippet: .. Treatment with DNase I eliminated the presence of released DNA, but did not block killing of the larvae by mouse neutrophils and macrophages in vitro. .. This observation suggests that in contrast to human neutrophils and macrophages, mouse cells do not require ET formation in vitro to kill the worms.

    Positive Control:

    Article Title: STAT3, STAT4, NFATc1, and CTCF regulate PD-1 through multiple novel regulatory regions in murine T cells
    Article Snippet: .. Concentration ranges for DNase I were determined empirically for each lot and cell type by titrating DNase I for its ability to digest CR-C as the positive control but not regions previously found to be resistant to DNase I (e.g., +6.3 region). .. Following purification of the digested DNA, PCR was performed across the Pdcd1 locus using a set of 59 primer pairs ( ).

    Blocking Assay:

    Article Title: Extracellular traps are associated with human and mouse neutrophil and macrophage mediated killing of larval Strongyloides stercoralis
    Article Snippet: .. Treatment with DNase I eliminated the presence of released DNA, but did not block killing of the larvae by mouse neutrophils and macrophages in vitro. .. This observation suggests that in contrast to human neutrophils and macrophages, mouse cells do not require ET formation in vitro to kill the worms.

    Concentration Assay:

    Article Title: Interactions of NBU1 IntN1 and Orf2x Proteins with Attachment Site DNA
    Article Snippet: .. DNase I (Worthington) diluted in DNase I dilution buffer (2.5 mM MgCl2 , 0.5 mM CaCl2 , 10 mM Tris-HCl, pH 7.6, 0.1 mg/ml BSA) to a final concentration of 0.0625 μg/ml was incubated with reaction mixtures for 1 min. ..

    Article Title: STAT3, STAT4, NFATc1, and CTCF regulate PD-1 through multiple novel regulatory regions in murine T cells
    Article Snippet: .. Concentration ranges for DNase I were determined empirically for each lot and cell type by titrating DNase I for its ability to digest CR-C as the positive control but not regions previously found to be resistant to DNase I (e.g., +6.3 region). .. Following purification of the digested DNA, PCR was performed across the Pdcd1 locus using a set of 59 primer pairs ( ).

    Incubation:

    Article Title: Epigenetic Control of Cell Cycle-Dependent Histone Gene Expression Is a Principal Component of the Abbreviated Pluripotent Cell Cycle
    Article Snippet: .. Nuclei were then resuspended in RSB buffer supplemented with 1 mM CaCl2 and incubated with increasing concentrations of DNase I (DPRF; Worthington Biochemical Corporation, Lakewood, NJ) for 10 min at room temperature with gentle agitation. ..

    Article Title: Interactions of NBU1 IntN1 and Orf2x Proteins with Attachment Site DNA
    Article Snippet: .. DNase I (Worthington) diluted in DNase I dilution buffer (2.5 mM MgCl2 , 0.5 mM CaCl2 , 10 mM Tris-HCl, pH 7.6, 0.1 mg/ml BSA) to a final concentration of 0.0625 μg/ml was incubated with reaction mixtures for 1 min. ..

    other:

    Article Title: Interactions of NBU1 IntN1 and Orf2x Proteins with Attachment Site DNA
    Article Snippet: The region is dA+dT rich, and protection seen from positions +126 to +129 is subtle because DNase I does not cleave DNA efficiently in this region.

    Article Title: Structural and functional conservation at the boundaries of the chicken ?-globin domain
    Article Snippet: The DNase I HS, 3′HS, was detected by digestion of nuclei with increasing amounts of DNase I as above followed by digestion of extracted DNA with Kpn I and probing with i112 (1000 bp; Kpn I –Pst I at 23.3–24.3 map units).