dnase i (Worthington Biochemical)


99
Name:
Deoxyribonuclease I
Description:
Chromatographically purified A lyophilized powder with glycine as a stabilizer
Catalog Number:
ls002004
Price:
33
Size:
5 mg
Source:
Bovine Pancreas
Cas Number:
9003.98.9
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Structured Review
Worthington Biochemical
dnase i

Chromatographically purified A lyophilized powder with glycine as a stabilizer
https://www.bioz.com/result/dnase i/product/Worthington Biochemical
Average 99 stars, based on 971 article reviews
Price from $9.99 to $1999.99

Chromatographically purified A lyophilized powder with glycine as a stabilizer
https://www.bioz.com/result/dnase i/product/Worthington Biochemical
Average 99 stars, based on 971 article reviews
Price from $9.99 to $1999.99
dnase i - by Bioz Stars,
2021-01
99/100 stars
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Images
1) Product Images from "Exclusion of NFAT5 from Mitotic Chromatin Resets Its Nucleo-Cytoplasmic Distribution in Interphase"
Article Title: Exclusion of NFAT5 from Mitotic Chromatin Resets Its Nucleo-Cytoplasmic Distribution in Interphase
Journal: PLoS ONE
doi: 10.1371/journal.pone.0007036

Figure Legend Snippet: Constitutive binding to interphase chromatin of an NFAT5a mutant lacking the CTD. (A) Flow cytometry analysis of the cell cycle profile ( left histogram ) and proportion of interphase and mitotic cells ( dot plot ) in asynchronous HEK293 cultures. (B) HEK293 cells cultured in isotonic medium (310 mOsm/kg) or exposed to hypertonic conditions (470 mOsm/kg) during 4 hours were lysed and proteins were fractionated into soluble (S) or chromatin (Ch) fractions. One set of samples was treated with DNase I during lysis, which caused the release of chromatin-associated proteins to the soluble fraction. NFAT5 and markers of soluble (pyruvate kinase) and chromatin-associated proteins (histone H3) were detected by Western blotting. (C) HEK293 cells expressing Myc-tagged full length NFAT5a (FL5), a DNA-binding mutant (FL5 DB1 ), or a construct comprising the amino-terminal region plus DNA-binding domain (ND5) (diagram in Figure 1B ) and its DNA-binding mutant (ND5 DB1 ) were cultured in isotonic medium (290 mOsm/kg) or exposed to hypertonic conditions (470 mOsm/kg) during 4 hours, lysed, and proteins were fractionated into soluble (S) or chromatin (Ch) fractions. NFAT5 constructs were detected by Western blotting with an anti-Myc antibody. Results shown are representative of four independent experiments.
Techniques Used: Binding Assay, Mutagenesis, Flow Cytometry, Cytometry, Cell Culture, Lysis, Western Blot, Expressing, Construct
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In Vitro:Article Title: Extracellular traps are associated with human and mouse neutrophil and macrophage mediated killing of larval Strongyloides stercoralis Article Snippet: .. Treatment with Positive Control:Article Title: STAT3, STAT4, NFATc1, and CTCF regulate PD-1 through multiple novel regulatory regions in murine T cells Article Snippet: .. Concentration ranges for Immunoprecipitation:Article Title: Association of Herpes Simplex Virus Type 1 ICP8 and ICP27 Proteins with Cellular RNA Polymerase II Holoenzyme Article Snippet: .. To investigate this hypothesis, we treated lysates of wt HSV-1 KOS strain-infected HEp-2 cells with a cocktail of RNase A and RNase T1 or with Footprinting:Article Title: Controller protein of restriction–modification system Kpn2I affects transcription of its gene by acting as a transcription elongation roadblock Article Snippet: .. To map the C.Kpn2I binding site we used Blocking Assay:Article Title: Extracellular traps are associated with human and mouse neutrophil and macrophage mediated killing of larval Strongyloides stercoralis Article Snippet: .. Treatment with Concentration Assay:Article Title: STAT3, STAT4, NFATc1, and CTCF regulate PD-1 through multiple novel regulatory regions in murine T cells Article Snippet: .. Concentration ranges for Incubation:Article Title: Epigenetic Control of Cell Cycle-Dependent Histone Gene Expression Is a Principal Component of the Abbreviated Pluripotent Cell Cycle Article Snippet: .. Nuclei were then resuspended in RSB buffer supplemented with 1 mM CaCl2 and incubated with increasing concentrations of Binding Assay:Article Title: Controller protein of restriction–modification system Kpn2I affects transcription of its gene by acting as a transcription elongation roadblock Article Snippet: .. To map the C.Kpn2I binding site we used |