xhoi  (New England Biolabs)


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  • 99
    Name:
    XhoI
    Description:
    XhoI 25 000 units
    Catalog Number:
    r0146l
    Price:
    290
    Size:
    25 000 units
    Category:
    Restriction Enzymes
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    Structured Review

    New England Biolabs xhoi
    XhoI
    XhoI 25 000 units
    https://www.bioz.com/result/xhoi/product/New England Biolabs
    Average 99 stars, based on 6407 article reviews
    Price from $9.99 to $1999.99
    xhoi - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title:
    Article Snippet: .. Restriction enzyme analysis of PCR products containing the CYP2B6 exon-9 region was performed by incubating 2 μ g DNA with 100 U XhoI (NEB) overnight. .. Total RNA was isolated using Trizol (Invitrogen).

    Article Title: A partial form of recessive STAT1 deficiency in humans
    Article Snippet: .. PCR products and empty pSPL3 plasmids (provided by Ralph Burkhardt, The Rockefeller University) were digested with Xho I and BamH 1 (New England BioLabs). .. Plasmids were then dephosphorylated, and 50 ng of purified plasmids and 250 ng of purified PCR products were ligated with T4 ligase (New England BioLabs).

    Article Title: A rationally designed nanoparticle for RNA interference therapy in B-lineage lymphoid malignancies
    Article Snippet: .. The correct PCR products (FL: 2541-bp and ΔE12–14: 2208-bp) were ligated into the 8497-bp lentiviral vector pCL6-2AEGwo through the NheI and XhoI restriction sites (underlined) using the Quick Ligase kit (New England Biolabs catalog no. M2200L) following the manufacturer's instructions. .. The pCL6-2AEGwo lentiviral backbone vector, a kind gift from Dr. Zanxian Xia, School of Biological Science and Technology, Central South University, Changsha, Hunan 410078, China, contains both a “ribosome-skip” fragment encoding the 2A-like peptide APVKQTLNFDLLKLAGDVESNPGP and an in-frame eGFP fluorescent coding sequence downstream of a multiple cloning site.

    Incubation:

    Article Title: Effects of size and topology of DNA molecules on intracellular delivery with non-viral gene carriers
    Article Snippet: .. Linear DNA (l-DNA) Purified c-DNA was linearized using the restriction enzyme, Xho I (New England Biolabs; Pickering, ON) for pORF9-hTNFRS11b or Cla I (Invitrogen; Burlington, ON) for pEGFP-N2, Restriction digestion were set up with 5 μg of DNA per 50 μL of reaction volume containing 3 units of enzyme and incubated at 37°C for 16 hours. .. The enzyme was then heat-inactivated by incubating the mixture at 65°C for 10 min. Digested DNA was purified using QIAEX II Gel Extraction Kit (Qiagen, Mississauga, ON).

    Agarose Gel Electrophoresis:

    Article Title: Retrofitting the BAC cloning vector pBeloBAC11 by the insertion of a mutant loxP site
    Article Snippet: .. The DNA samples were digested with 10 U of Xho I for 2 h at 37 °C (New England BioLabs) and electrophoresed in a 0.8% agarose gel in 0.5X TBE. .. Results Since the BAC cloning vector pBeloBAc11 was commercially available from New England BioLabs, I decided to modify this plasmid to increase its utility for genomics studies.

    Plasmid Preparation:

    Article Title: A rationally designed nanoparticle for RNA interference therapy in B-lineage lymphoid malignancies
    Article Snippet: .. The correct PCR products (FL: 2541-bp and ΔE12–14: 2208-bp) were ligated into the 8497-bp lentiviral vector pCL6-2AEGwo through the NheI and XhoI restriction sites (underlined) using the Quick Ligase kit (New England Biolabs catalog no. M2200L) following the manufacturer's instructions. .. The pCL6-2AEGwo lentiviral backbone vector, a kind gift from Dr. Zanxian Xia, School of Biological Science and Technology, Central South University, Changsha, Hunan 410078, China, contains both a “ribosome-skip” fragment encoding the 2A-like peptide APVKQTLNFDLLKLAGDVESNPGP and an in-frame eGFP fluorescent coding sequence downstream of a multiple cloning site.

    Article Title: A Telomeric Avirulence Gene Determines Efficacy for the Rice Blast Resistance Gene Pi-ta
    Article Snippet: .. Plasmid pCB783, containing the 791-bp telomeric SacI fragment, was digested first with KpnI and XhoI and then with Exonuclease III (New England Biolabs). .. The ExoIII-digested DNAs were treated with S1 nuclease (Sigma) followed by a fill-in reaction with the Klenow fragment of DNA polymerase I, ligation, and transformation into DH5αMCR cells (Gibco BRL).

    Purification:

    Article Title: Effects of size and topology of DNA molecules on intracellular delivery with non-viral gene carriers
    Article Snippet: .. Linear DNA (l-DNA) Purified c-DNA was linearized using the restriction enzyme, Xho I (New England Biolabs; Pickering, ON) for pORF9-hTNFRS11b or Cla I (Invitrogen; Burlington, ON) for pEGFP-N2, Restriction digestion were set up with 5 μg of DNA per 50 μL of reaction volume containing 3 units of enzyme and incubated at 37°C for 16 hours. .. The enzyme was then heat-inactivated by incubating the mixture at 65°C for 10 min. Digested DNA was purified using QIAEX II Gel Extraction Kit (Qiagen, Mississauga, ON).

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