bsa  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    BSA-Molecular Biology Grade
    Description:

    Catalog Number:
    B9000S
    Price:
    None
    Score:
    85
    Buy from Supplier


    Structured Review

    New England Biolabs bsa

    https://www.bioz.com/result/bsa/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bsa - by Bioz Stars, 2019-12
    99/100 stars

    Related Products / Commonly Used Together

    fbs
    transferrin
    imdm

    Images

    Related Articles

    Centrifugation:

    Article Title: YY1 Is a Structural Regulator of Enhancer-Promoter Loops
    Article Snippet: Nuclei were pelleted by centrifugation at 2500 r cf. for 5 min at 4°C and washed once with 500 μL of ice-cold Hi-C lysis buffer. .. Proximity ligation was performed by addition of 947 μL of ligation master mix, containing 150 μL of 10X NEB T4 DNA ligase buffer (NEB, B0202), 125 μL of 10% Triton X-100, 7.5 μL of 20 mg/mL BSA (NEB, B9000), 10 μL of 400 U/μL T4 DNA ligase (NEB, M0202), and 655.5 μL of water, and incubation at room temperature for 4 hours with rotation.

    Amplification:

    Article Title: Quantification and localization of oncogenic receptor tyrosine kinase variant transcripts using molecular inversion probes
    Article Snippet: Paragraph title: Padlock probe rolling circle amplification ... BNA primer hybridization and mRNA reverse transcription were performed o/n at 42 °C using 200 nM of BNA primer (IDT, Leuven Belgium), 20 U/µl RevertAid H minus M-MuLV reverse transcriptase in 1x M-MuLV reaction buffer (ThermoFisher Scientific, Waltham, MA, USA), 1 U/µl RiboLock RNase Inhibitor (ThermoFisher Scientific, Waltham, MA, USA), 500 µM dNTP and 0.2 µg/µl BSA (New England Biolabs, Ipswich, MA, USA).

    Article Title: The MIRA method for DNA methylation analysis
    Article Snippet: Paragraph title: 2.2. MIRA procedure and amplicon generation ... 1 MseI enzyme, NEB 2 buffer and 1 mg/mL BSA (New England Biolabs; Ipswich, MA).

    Article Title: Association between two ?-opioid receptor gene (OPRM1) haplotype blocks and drug or alcohol dependence
    Article Snippet: All other SNPs were genotyped using the TaqMan assay in a 384-well microplate format. .. Briefly, 1 ng of DNA was amplified in a final volume of 2 μl containing 0.05 μl of 20× (or 0.025 μl of 40×) MGB probes and primers, 1 μl of 2× TaqMan Universal PCR Master Mix, and 0.004 μl of 100× BSA (New England Biolabs Inc., Beverly, MA). .. Amplification conditions were 95°C for 10 min, followed by 60 cycles of 92°C for 15 s and 60°C for 1 min. Allelic discrimination was performed using the ABI PRISM® 7900 Sequence Detection System (Applied Biosystems Inc., Foster City, CA, USA).

    Synthesized:

    Article Title: Identification of Grass-specific Enzyme That Acylates Monolignols with p-Coumarate
    Article Snippet: Authentic coniferyl p -coumarate 3Ga and sinapyl p -coumarate 3Sa were synthesized as described previously ( ). p -Coumaryl p -coumarate 3Ha and sinapyl acetate were made by an analogous route ( ). .. The OsPMT enzyme activity assay, in 50 m m sodium phosphate buffer, pH 6, containing 1 m m dithiothreitol (DTT), 1 m m CoA thioester, 1 m m monolignol, and deionized water to produce a final volume of 50 μl, was initiated by adding of 1 μg of purified PMT protein in 1× BSA (NEB).

    Article Title: Mutation identification in a canine model of X-linked ectodermal dysplasia
    Article Snippet: Linkage analysis was performed using the computer program FASTLINK ( ; ; ). .. Total RNA was extracted from skin and kidney from normal and affected dogs using TRIzol reagent (Life Technologies, Grand Island, NY) according to the manufacturer’s protocol. cDNA was synthesized in a 50-μl reaction containing 10 μg total RNA 1 × first-strand synthesis buffer (Invitrogen, Carlsbad, CA), 0.1 mM DTT (Invitrogen), 8 mM dNTPs (Promega, Madison, WI), 0.2 μg/μl BSA (NEB), 8 μM random hexamers (Promega), 8 μM oligo d(T) (Promega), 80 U RNAsin (Promega), and 500 U Superscript II reverse transcriptase (Invitrogen). .. PCRs were carried out according to the protocols described below, using 1 μl of cDNA as the template for each reaction.

    ChIA Pet Assay:

    Article Title: Evolutionarily Conserved Principles Predict 3D Chromatin Organization
    Article Snippet: Paragraph title: Hi-C, ChIA-PET, and HiChIP Library Preparation and Processing ... This reaction was placed at 37°C for 1.5 h, after which samples were ligated for 4 h at room temperature with addition of 663 µl H2 O, 120 µl 10× NEB T4 DNA Ligase buffer, 100 µl 10% Triton X-100, 12 µl 10 mg/ml BSA, and 5 µl 400 u/µl T4 DNA Ligase (NEB M0202).

    Construct:

    Article Title: Specific DNA-binding by Apicomplexan AP2 transcription factors
    Article Snippet: Purified proteins were diluted to a final concentration of 100–500 nM in PBS, 2% (wt/vol) milk, 51.3 ng/μl salmon testes DNA (Sigma), 0.2 μg/μl BSA (New England Biolabs), and incubated for 1 h at 20°C. .. After washing, specific DNA–protein interactions were visualized by using a GSI Lumonics ScanArray 5000 scanner to detect fluorescence from an Alexa488-conjugated anti-GST antibody.

    Article Title: Common and Distinguishing Regulatory and Expression Characteristics of the Highly Related KorB Proteins of Streptomycete Plasmids pIJ101 and pSB24.2
    Article Snippet: Ampicillin was included at 50 μg/ml when plasmid-containing E. coli strains were being grown ( ); when Streptomyces strains containing resistance-encoding plasmids were being grown, thiostrepton was included at a concentration of 50 or 5 μg/ml in solid or liquid media, respectively, and hygromycin was included at a concentration of 200 or 20 μg/ml in solid or liquid media, respectively. .. Standard molecular biology protocols ( ) and enzymes purchased from New England BioLabs or Invitrogen were used to construct various pGSP and pSCON plasmids listed in Table . .. PCR performed as described by Pettis et al. ( ) was used to construct plasmids pSCON20 and pSCON30, except that plasmid pGSP368 was the amplification template and that Pfu polymerase (Stratagene) was used. pSCON20 was made by first amplifying a 283-bp DNA fragment by using primers korB.eq-5 (5′-AAAAATCTAGAGCCCATGACGCAGGCTGACAC-3′) and revkorB242 (5′-AAAAACTCGAGCTAGCTTCCGGAGCCCTTCTC-3′); the resulting product was extracted and precipitated as previously described ( ) and was then digested to completion with Xba I and Xho I and was ligated into pSP72 at these sites. pSCON30 was constructed by amplifying a 266-bp DNA fragment by using primers BamkrB242F (5′-AAAAAAGGATCCATGACGCACAAGACACCG-3′) and XhokrB242R (5′-AAAAACTCGAGCTAGCTTCCGGAGCCCTTC-3′); subsequently, this product was treated in a manner similar to that for the fragment used to create pSCON20, except that digestion was with Bam HI and Xho I and that ligation was into pET30a+ at these sites so that korB (pSB24.2) was in frame with the N-terminal His6 tag.

    Real-time Polymerase Chain Reaction:

    Article Title: Editing DNA methylation in the mammalian genome
    Article Snippet: Then 713 µl H2O, 120 µl 10 x T4 DNA ligase buffer (NEB, B0202), 100 µl 10% Triton X-100, 12 µl 10 mg/ml BSA, and 5 µl T4 DNA ligase (NEB, M0202) were added and incubated for 22 hour at 16 °C. .. Then 713 µl H2O, 120 µl 10 x T4 DNA ligase buffer (NEB, B0202), 100 µl 10% Triton X-100, 12 µl 10 mg/ml BSA, and 5 µl T4 DNA ligase (NEB, M0202) were added and incubated for 22 hour at 16 °C.

    Microarray:

    Article Title: Specific DNA-binding by Apicomplexan AP2 transcription factors
    Article Snippet: Primer extension from a universal 24-mer region on all 60-mers generates a double-stranded DNA microarray platform. .. Purified proteins were diluted to a final concentration of 100–500 nM in PBS, 2% (wt/vol) milk, 51.3 ng/μl salmon testes DNA (Sigma), 0.2 μg/μl BSA (New England Biolabs), and incubated for 1 h at 20°C.

    Incubation:

    Article Title: Editing DNA methylation in the mammalian genome
    Article Snippet: 25 µl 10 x NEB buffer 2 and 100 U BglII (NEB, R0144S) were added to digest chromatin over night at 37 °C. .. Then 713 µl H2O, 120 µl 10 x T4 DNA ligase buffer (NEB, B0202), 100 µl 10% Triton X-100, 12 µl 10 mg/ml BSA, and 5 µl T4 DNA ligase (NEB, M0202) were added and incubated for 22 hour at 16 °C. .. The chromatin was reverse cross-linked, and DNA was purified by phenol:chloroform:isoamyl alcohol (Sigma, P3803) extraction.

    Article Title: Quantification and localization of oncogenic receptor tyrosine kinase variant transcripts using molecular inversion probes
    Article Snippet: BNA primer hybridization and mRNA reverse transcription were performed o/n at 42 °C using 200 nM of BNA primer (IDT, Leuven Belgium), 20 U/µl RevertAid H minus M-MuLV reverse transcriptase in 1x M-MuLV reaction buffer (ThermoFisher Scientific, Waltham, MA, USA), 1 U/µl RiboLock RNase Inhibitor (ThermoFisher Scientific, Waltham, MA, USA), 500 µM dNTP and 0.2 µg/µl BSA (New England Biolabs, Ipswich, MA, USA). .. BNA primer hybridization and mRNA reverse transcription were performed o/n at 42 °C using 200 nM of BNA primer (IDT, Leuven Belgium), 20 U/µl RevertAid H minus M-MuLV reverse transcriptase in 1x M-MuLV reaction buffer (ThermoFisher Scientific, Waltham, MA, USA), 1 U/µl RiboLock RNase Inhibitor (ThermoFisher Scientific, Waltham, MA, USA), 500 µM dNTP and 0.2 µg/µl BSA (New England Biolabs, Ipswich, MA, USA).

    Article Title: Specific DNA-binding by Apicomplexan AP2 transcription factors
    Article Snippet: Primer extension from a universal 24-mer region on all 60-mers generates a double-stranded DNA microarray platform. .. Purified proteins were diluted to a final concentration of 100–500 nM in PBS, 2% (wt/vol) milk, 51.3 ng/μl salmon testes DNA (Sigma), 0.2 μg/μl BSA (New England Biolabs), and incubated for 1 h at 20°C. .. After washing, specific DNA–protein interactions were visualized by using a GSI Lumonics ScanArray 5000 scanner to detect fluorescence from an Alexa488-conjugated anti-GST antibody.

    Article Title: YY1 Is a Structural Regulator of Enhancer-Promoter Loops
    Article Snippet: Following digestion, MboI enzyme was heat inactivated by incubating the nuclei at 62°C for 20 min. To fill in the restriction fragment overhangs and mark the DNA ends with biotin, 52 μL of fill-in master mix, containing 37.5 μL of 0.4 mM biotin-dATP (Invitrogen, 19524016), 1.5 μL of 10 mM dCTP (Invitrogen, 18253013), 1.5 μL of 10 mM dGTP (Invitrogen, 18254011), 1.5 μL of 10 mM dTTP (Invitrogen, 18255018), and 10 μL of 5 U/μL DNA Polymerase I, Large (Klenow) Fragment (NEB, M0210), was added and the tubes were incubated at 37°C for 1 hour with rotation. .. Proximity ligation was performed by addition of 947 μL of ligation master mix, containing 150 μL of 10X NEB T4 DNA ligase buffer (NEB, B0202), 125 μL of 10% Triton X-100, 7.5 μL of 20 mg/mL BSA (NEB, B9000), 10 μL of 400 U/μL T4 DNA ligase (NEB, M0202), and 655.5 μL of water, and incubation at room temperature for 4 hours with rotation. .. After proximity ligation, nuclei were pelleted by centrifugation at 2500 r cf. for 5 minutes and resuspended in 1 mL of ChIP sonication buffer (50 mM HEPES-KOH pH 7.5, 140 mM NaCl, 1 mM EDTA pH 8.0, 1 mM EGTA pH 8.0, 1% Triton X-100, 0.1% sodium deoxycholate, and 0.1% SDS with protease inhibitor).

    Formalin-fixed Paraffin-Embedded:

    Article Title: A distinct subset of Atypical Spitz Tumors is characterized by BRAF mutation and loss of BAP1 expression
    Article Snippet: DNA was extracted and purified with a QIAamp DNA FFPE Tissue Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions. .. BSA (New England Biolabs), 1 U Hotstar Taq (Qiagen), 1× Hotstar Taq buffer (Qiagen) and 0.4 µM primer.

    Article Title: Quantification and localization of oncogenic receptor tyrosine kinase variant transcripts using molecular inversion probes
    Article Snippet: BNA primer hybridization and mRNA reverse transcription were performed o/n at 42 °C using 200 nM of BNA primer (IDT, Leuven Belgium), 20 U/µl RevertAid H minus M-MuLV reverse transcriptase in 1x M-MuLV reaction buffer (ThermoFisher Scientific, Waltham, MA, USA), 1 U/µl RiboLock RNase Inhibitor (ThermoFisher Scientific, Waltham, MA, USA), 500 µM dNTP and 0.2 µg/µl BSA (New England Biolabs, Ipswich, MA, USA). .. BNA primer hybridization and mRNA reverse transcription were performed o/n at 42 °C using 200 nM of BNA primer (IDT, Leuven Belgium), 20 U/µl RevertAid H minus M-MuLV reverse transcriptase in 1x M-MuLV reaction buffer (ThermoFisher Scientific, Waltham, MA, USA), 1 U/µl RiboLock RNase Inhibitor (ThermoFisher Scientific, Waltham, MA, USA), 500 µM dNTP and 0.2 µg/µl BSA (New England Biolabs, Ipswich, MA, USA).

    Footprinting:

    Article Title: Secondary structure and DNA binding by the C-terminal domain of the transcriptional activator NifA from Klebsiella pneumoniae
    Article Snippet: Paragraph title: DNase I footprinting ... Reactions for the binding assays contained 5 nM DNA, 8.1 ng/µl poly(dI–dC) (Boehringer Mannheim), 25 mM Tris acetate pH 8, 50 mM potassium acetate, 13.5 mM ammonium acetate, 0.5 µM acetylated BSA (NEB) and 2.5–7.5 µM NifA C-terminal DNA-binding domain in a final volume of 15 µl.

    Expressing:

    Article Title: Engineering yeast for the production of breviscapine by genomic analysis and synthetic biology approaches
    Article Snippet: T4 ligase, Bsa I, and 100 × bovine serum albumin (BSA) were purchased from NEB, USA. .. T4 ligase, Bsa I, and 100 × bovine serum albumin (BSA) were purchased from NEB, USA.

    Modification:

    Article Title: Identification of Grass-specific Enzyme That Acylates Monolignols with p-Coumarate
    Article Snippet: These CoA thioesters were purified using Sep-Pak cartridges (Waters Corporation) following a method modified from Beuerle and Pichersky ( ). .. The OsPMT enzyme activity assay, in 50 m m sodium phosphate buffer, pH 6, containing 1 m m dithiothreitol (DTT), 1 m m CoA thioester, 1 m m monolignol, and deionized water to produce a final volume of 50 μl, was initiated by adding of 1 μg of purified PMT protein in 1× BSA (NEB).

    Western Blot:

    Article Title: Specific DNA-binding by Apicomplexan AP2 transcription factors
    Article Snippet: Purity was verified by silver stain SDS/PAGE and Western blot analysis with an anti-GST antibody (Invitrogen). .. Purified proteins were diluted to a final concentration of 100–500 nM in PBS, 2% (wt/vol) milk, 51.3 ng/μl salmon testes DNA (Sigma), 0.2 μg/μl BSA (New England Biolabs), and incubated for 1 h at 20°C.

    Transformation Assay:

    Article Title: Common and Distinguishing Regulatory and Expression Characteristics of the Highly Related KorB Proteins of Streptomycete Plasmids pIJ101 and pSB24.2
    Article Snippet: Standard molecular biology protocols ( ) and enzymes purchased from New England BioLabs or Invitrogen were used to construct various pGSP and pSCON plasmids listed in Table . .. Standard molecular biology protocols ( ) and enzymes purchased from New England BioLabs or Invitrogen were used to construct various pGSP and pSCON plasmids listed in Table .

    Hybridization:

    Article Title: Quantification and localization of oncogenic receptor tyrosine kinase variant transcripts using molecular inversion probes
    Article Snippet: In situ reverse transcription was performed using BNA primers positioned within ≤1 nt from the 5′ end of the padlock probe target site (see Table for nucleotide sequences). .. BNA primer hybridization and mRNA reverse transcription were performed o/n at 42 °C using 200 nM of BNA primer (IDT, Leuven Belgium), 20 U/µl RevertAid H minus M-MuLV reverse transcriptase in 1x M-MuLV reaction buffer (ThermoFisher Scientific, Waltham, MA, USA), 1 U/µl RiboLock RNase Inhibitor (ThermoFisher Scientific, Waltham, MA, USA), 500 µM dNTP and 0.2 µg/µl BSA (New England Biolabs, Ipswich, MA, USA). .. Post-fixation was performed with 3.7% formaldehyde (5 min) or 3.7% PFA (45 min) for cells or tissues, respectively.

    Electroporation:

    Article Title: Common and Distinguishing Regulatory and Expression Characteristics of the Highly Related KorB Proteins of Streptomycete Plasmids pIJ101 and pSB24.2
    Article Snippet: Standard molecular biology protocols ( ) and enzymes purchased from New England BioLabs or Invitrogen were used to construct various pGSP and pSCON plasmids listed in Table . .. PCR performed as described by Pettis et al. ( ) was used to construct plasmids pSCON20 and pSCON30, except that plasmid pGSP368 was the amplification template and that Pfu polymerase (Stratagene) was used. pSCON20 was made by first amplifying a 283-bp DNA fragment by using primers korB.eq-5 (5′-AAAAATCTAGAGCCCATGACGCAGGCTGACAC-3′) and revkorB242 (5′-AAAAACTCGAGCTAGCTTCCGGAGCCCTTCTC-3′); the resulting product was extracted and precipitated as previously described ( ) and was then digested to completion with Xba I and Xho I and was ligated into pSP72 at these sites. pSCON30 was constructed by amplifying a 266-bp DNA fragment by using primers BamkrB242F (5′-AAAAAAGGATCCATGACGCACAAGACACCG-3′) and XhokrB242R (5′-AAAAACTCGAGCTAGCTTCCGGAGCCCTTC-3′); subsequently, this product was treated in a manner similar to that for the fragment used to create pSCON20, except that digestion was with Bam HI and Xho I and that ligation was into pET30a+ at these sites so that korB (pSB24.2) was in frame with the N-terminal His6 tag.

    Flow Cytometry:

    Article Title: Identification of Grass-specific Enzyme That Acylates Monolignols with p-Coumarate
    Article Snippet: The OsPMT enzyme activity assay, in 50 m m sodium phosphate buffer, pH 6, containing 1 m m dithiothreitol (DTT), 1 m m CoA thioester, 1 m m monolignol, and deionized water to produce a final volume of 50 μl, was initiated by adding of 1 μg of purified PMT protein in 1× BSA (NEB). .. The OsPMT enzyme activity assay, in 50 m m sodium phosphate buffer, pH 6, containing 1 m m dithiothreitol (DTT), 1 m m CoA thioester, 1 m m monolignol, and deionized water to produce a final volume of 50 μl, was initiated by adding of 1 μg of purified PMT protein in 1× BSA (NEB).

    Chromatography:

    Article Title: Engineering yeast for the production of breviscapine by genomic analysis and synthetic biology approaches
    Article Snippet: Chromatography-grade formic acid and isopropanol were obtained from Thermo Fisher Scientific, USA. .. T4 ligase, Bsa I, and 100 × bovine serum albumin (BSA) were purchased from NEB, USA.

    Article Title: Direct contacts between conserved motifs of different subunits provide major contribution to active site organization in human and mycobacterial dUTPases
    Article Snippet: To provide novel insights into the mechanism of this important and unique enzyme, with potential focus on the role of the metal co-factor, we decided to investigate the contribution of Motif I aspartate to structure and function. .. Materials for electrophoresis or chromatography were from Bio-Rad or Amersham Biosciences, phenol red from Merck, molecular biology products were from New England BioLabs or Fermentas. .. Other materials were from Sigma or Stratagene.

    Ligation:

    Article Title: YY1 Is a Structural Regulator of Enhancer-Promoter Loops
    Article Snippet: Following digestion, MboI enzyme was heat inactivated by incubating the nuclei at 62°C for 20 min. To fill in the restriction fragment overhangs and mark the DNA ends with biotin, 52 μL of fill-in master mix, containing 37.5 μL of 0.4 mM biotin-dATP (Invitrogen, 19524016), 1.5 μL of 10 mM dCTP (Invitrogen, 18253013), 1.5 μL of 10 mM dGTP (Invitrogen, 18254011), 1.5 μL of 10 mM dTTP (Invitrogen, 18255018), and 10 μL of 5 U/μL DNA Polymerase I, Large (Klenow) Fragment (NEB, M0210), was added and the tubes were incubated at 37°C for 1 hour with rotation. .. Proximity ligation was performed by addition of 947 μL of ligation master mix, containing 150 μL of 10X NEB T4 DNA ligase buffer (NEB, B0202), 125 μL of 10% Triton X-100, 7.5 μL of 20 mg/mL BSA (NEB, B9000), 10 μL of 400 U/μL T4 DNA ligase (NEB, M0202), and 655.5 μL of water, and incubation at room temperature for 4 hours with rotation. .. After proximity ligation, nuclei were pelleted by centrifugation at 2500 r cf. for 5 minutes and resuspended in 1 mL of ChIP sonication buffer (50 mM HEPES-KOH pH 7.5, 140 mM NaCl, 1 mM EDTA pH 8.0, 1 mM EGTA pH 8.0, 1% Triton X-100, 0.1% sodium deoxycholate, and 0.1% SDS with protease inhibitor).

    Protease Inhibitor:

    Article Title: Evolutionarily Conserved Principles Predict 3D Chromatin Organization
    Article Snippet: Cells were pelleted and resuspended in 500 µl cold Hi-C lysis buffer (10 mM Tris-HCl pH8, 10 mM NaCl, 0.2% Igepal CA-630, and 1× Protease Inhibitor (Roche 11873580001) and incubated on ice for 1 h. Nuclei were pelleted at 2500 rcf for 5 min at 4°C, resuspended in 100 µl 0. .. This reaction was placed at 37°C for 1.5 h, after which samples were ligated for 4 h at room temperature with addition of 663 µl H2 O, 120 µl 10× NEB T4 DNA Ligase buffer, 100 µl 10% Triton X-100, 12 µl 10 mg/ml BSA, and 5 µl 400 u/µl T4 DNA Ligase (NEB M0202).

    Article Title: YY1 Is a Structural Regulator of Enhancer-Promoter Loops
    Article Snippet: Cross-linked cell pellets were thawed on ice, resuspended in 800 μL of ice-cold Hi-C lysis buffer (10 mM Tris-HCl pH 8.0, 10 mM NaCl, and 0.2% IGEPAL CA-630 with 1× cOmplete protease inhibitor (Roche, 11697498001)), and incubated at 4°C for 30 minutes with rotation. .. Proximity ligation was performed by addition of 947 μL of ligation master mix, containing 150 μL of 10X NEB T4 DNA ligase buffer (NEB, B0202), 125 μL of 10% Triton X-100, 7.5 μL of 20 mg/mL BSA (NEB, B9000), 10 μL of 400 U/μL T4 DNA ligase (NEB, M0202), and 655.5 μL of water, and incubation at room temperature for 4 hours with rotation.

    Dissection:

    Article Title: A distinct subset of Atypical Spitz Tumors is characterized by BRAF mutation and loss of BAP1 expression
    Article Snippet: Tumor and non-tumor areas were separately microdissected from sections of archival paraffin-embedded tissue using a dissection microscope. .. BSA (New England Biolabs), 1 U Hotstar Taq (Qiagen), 1× Hotstar Taq buffer (Qiagen) and 0.4 µM primer.

    SDS Page:

    Article Title: Specific DNA-binding by Apicomplexan AP2 transcription factors
    Article Snippet: Purity was verified by silver stain SDS/PAGE and Western blot analysis with an anti-GST antibody (Invitrogen). .. Purified proteins were diluted to a final concentration of 100–500 nM in PBS, 2% (wt/vol) milk, 51.3 ng/μl salmon testes DNA (Sigma), 0.2 μg/μl BSA (New England Biolabs), and incubated for 1 h at 20°C.

    Generated:

    Article Title: Quantification and localization of oncogenic receptor tyrosine kinase variant transcripts using molecular inversion probes
    Article Snippet: BNA primer hybridization and mRNA reverse transcription were performed o/n at 42 °C using 200 nM of BNA primer (IDT, Leuven Belgium), 20 U/µl RevertAid H minus M-MuLV reverse transcriptase in 1x M-MuLV reaction buffer (ThermoFisher Scientific, Waltham, MA, USA), 1 U/µl RiboLock RNase Inhibitor (ThermoFisher Scientific, Waltham, MA, USA), 500 µM dNTP and 0.2 µg/µl BSA (New England Biolabs, Ipswich, MA, USA). .. BNA primer hybridization and mRNA reverse transcription were performed o/n at 42 °C using 200 nM of BNA primer (IDT, Leuven Belgium), 20 U/µl RevertAid H minus M-MuLV reverse transcriptase in 1x M-MuLV reaction buffer (ThermoFisher Scientific, Waltham, MA, USA), 1 U/µl RiboLock RNase Inhibitor (ThermoFisher Scientific, Waltham, MA, USA), 500 µM dNTP and 0.2 µg/µl BSA (New England Biolabs, Ipswich, MA, USA).

    Polymerase Chain Reaction:

    Article Title: A distinct subset of Atypical Spitz Tumors is characterized by BRAF mutation and loss of BAP1 expression
    Article Snippet: Mutations in BAP1 (all exons), BRAF (exon 15), and HRAS (exons 1 and 2) were determined by direct sequencing using previously described primers., The PCR reaction conditions were 0.25 mM dNTPs, 0.4. .. BSA (New England Biolabs), 1 U Hotstar Taq (Qiagen), 1× Hotstar Taq buffer (Qiagen) and 0.4 µM primer.

    Article Title: Staphylococcus aureus Cas9 is a multiple-turnover enzyme
    Article Snippet: Taken together, these findings suggest that SauCas9 may be an attractive alternative or complement to SpyCas9 and could possibly be leveraged for future biotechnological and therapeutic applications. .. S. pyogenes Cas9 (# M0386M), EnGen sgRNA Synthesis Kit, S. pyogenes (# E3322S), HiScribe T7 High Yield RNA Synthesis Kit (E2040S), 2× RNA Loading Dye (# B0363S), Nucleoside Digestion Mix (M0649S), Q5 Hot Start High-Fidelity 2× Master Mix (# M0494L), Monarch PCR & DNA Cleanup Kit (# T1030L), Proteinase K (# P8107S), Shrimp Alkaline Phosphatase (# M0371S), T4 Polynucleotide Kinase (# M0201S), Streptavidin Magnetic Beads (# S1420S), and NEBuffer 3.1 (# B7203S) with a 1× composition of 100 mM NaCl, 50 mM Tris-HCl, 10 mM MgCl2 , 100 μg/ml BSA, pH 7.9 at 25°C were all from New England Biolabs. .. Both S. pyogenes and S. aureus Cas9 were purified at New England Biolabs using standard liquid chromatography protein purification techniques.

    Article Title: Association between two ?-opioid receptor gene (OPRM1) haplotype blocks and drug or alcohol dependence
    Article Snippet: All other SNPs were genotyped using the TaqMan assay in a 384-well microplate format. .. Briefly, 1 ng of DNA was amplified in a final volume of 2 μl containing 0.05 μl of 20× (or 0.025 μl of 40×) MGB probes and primers, 1 μl of 2× TaqMan Universal PCR Master Mix, and 0.004 μl of 100× BSA (New England Biolabs Inc., Beverly, MA). .. Amplification conditions were 95°C for 10 min, followed by 60 cycles of 92°C for 15 s and 60°C for 1 min. Allelic discrimination was performed using the ABI PRISM® 7900 Sequence Detection System (Applied Biosystems Inc., Foster City, CA, USA).

    Article Title: Differential and Cross-Transcriptional Control of Duplicated Genes Encoding Alternative Sigma Factors in Streptomyces ambofaciens
    Article Snippet: Cosmid and plasmid DNAs were extracted from E. coli by the alkaline method ( ). .. All restriction enzymes, PCR reagents, and other molecular biology reagents were purchased from New England Biolabs or Roche Diagnostics. .. Digoxigenin-DNA labeling (digoxigenin dUTP), hybridization, washings, and detection were performed according to the recommendations of the manufacturer (Roche).

    DNA Sequencing:

    Article Title: Differential and Cross-Transcriptional Control of Duplicated Genes Encoding Alternative Sigma Factors in Streptomyces ambofaciens
    Article Snippet: All restriction enzymes, PCR reagents, and other molecular biology reagents were purchased from New England Biolabs or Roche Diagnostics. .. All restriction enzymes, PCR reagents, and other molecular biology reagents were purchased from New England Biolabs or Roche Diagnostics.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Mutation identification in a canine model of X-linked ectodermal dysplasia
    Article Snippet: Paragraph title: cDNA synthesis and RT-PCR ... Total RNA was extracted from skin and kidney from normal and affected dogs using TRIzol reagent (Life Technologies, Grand Island, NY) according to the manufacturer’s protocol. cDNA was synthesized in a 50-μl reaction containing 10 μg total RNA 1 × first-strand synthesis buffer (Invitrogen, Carlsbad, CA), 0.1 mM DTT (Invitrogen), 8 mM dNTPs (Promega, Madison, WI), 0.2 μg/μl BSA (NEB), 8 μM random hexamers (Promega), 8 μM oligo d(T) (Promega), 80 U RNAsin (Promega), and 500 U Superscript II reverse transcriptase (Invitrogen).

    Sonication:

    Article Title: The MIRA method for DNA methylation analysis
    Article Snippet: 1 MseI enzyme, NEB 2 buffer and 1 mg/mL BSA (New England Biolabs; Ipswich, MA). .. 1 MseI enzyme, NEB 2 buffer and 1 mg/mL BSA (New England Biolabs; Ipswich, MA).

    Article Title: YY1 Is a Structural Regulator of Enhancer-Promoter Loops
    Article Snippet: Proximity ligation was performed by addition of 947 μL of ligation master mix, containing 150 μL of 10X NEB T4 DNA ligase buffer (NEB, B0202), 125 μL of 10% Triton X-100, 7.5 μL of 20 mg/mL BSA (NEB, B9000), 10 μL of 400 U/μL T4 DNA ligase (NEB, M0202), and 655.5 μL of water, and incubation at room temperature for 4 hours with rotation. .. After proximity ligation, nuclei were pelleted by centrifugation at 2500 r cf. for 5 minutes and resuspended in 1 mL of ChIP sonication buffer (50 mM HEPES-KOH pH 7.5, 140 mM NaCl, 1 mM EDTA pH 8.0, 1 mM EGTA pH 8.0, 1% Triton X-100, 0.1% sodium deoxycholate, and 0.1% SDS with protease inhibitor).

    Binding Assay:

    Article Title: Secondary structure and DNA binding by the C-terminal domain of the transcriptional activator NifA from Klebsiella pneumoniae
    Article Snippet: The 341 bp Eco RI– Bam HI fragment of pMB1 ( , ) containing three UASs from the nifH – nifJ intergenic region, was 5′-end labelled using [γ-32 P]dATP and T4 polynucleotide kinase. .. Reactions for the binding assays contained 5 nM DNA, 8.1 ng/µl poly(dI–dC) (Boehringer Mannheim), 25 mM Tris acetate pH 8, 50 mM potassium acetate, 13.5 mM ammonium acetate, 0.5 µM acetylated BSA (NEB) and 2.5–7.5 µM NifA C-terminal DNA-binding domain in a final volume of 15 µl. .. The reaction mixes were incubated at 30°C for 20 min. DNase I (1.1 × 10–4 U; Boehringer Mannheim) was added in 3 µl of buffer containing 10 mM Tris–HCl, 10 mM CaCl2 , 40 mM MgCl2 and 10% glycerol.

    Hi-C:

    Article Title: Evolutionarily Conserved Principles Predict 3D Chromatin Organization
    Article Snippet: Paragraph title: Hi-C, ChIA-PET, and HiChIP Library Preparation and Processing ... This reaction was placed at 37°C for 1.5 h, after which samples were ligated for 4 h at room temperature with addition of 663 µl H2 O, 120 µl 10× NEB T4 DNA Ligase buffer, 100 µl 10% Triton X-100, 12 µl 10 mg/ml BSA, and 5 µl 400 u/µl T4 DNA Ligase (NEB M0202).

    Article Title: YY1 Is a Structural Regulator of Enhancer-Promoter Loops
    Article Snippet: Nuclei were pelleted by centrifugation at 2500 r cf. for 5 min at 4°C and washed once with 500 μL of ice-cold Hi-C lysis buffer. .. Proximity ligation was performed by addition of 947 μL of ligation master mix, containing 150 μL of 10X NEB T4 DNA ligase buffer (NEB, B0202), 125 μL of 10% Triton X-100, 7.5 μL of 20 mg/mL BSA (NEB, B9000), 10 μL of 400 U/μL T4 DNA ligase (NEB, M0202), and 655.5 μL of water, and incubation at room temperature for 4 hours with rotation.

    Molecular Weight:

    Article Title: Reconstituting the 4-Strand DNA Strand Exchange
    Article Snippet: Standard agarose gel running setup (Bio-Rad Wide Mini-Sub Cell GT) AlphaImager 3400 gel documentation station (Alpha Innotech) Vortex-2 Genie (Scientific Industries) Low-speed benchtop centrifuge Heat blocks or water baths set at 37°C and 65°C UV lamp Electroelution apparatus: Schleicher & Schuell Elutrap (Whatman) Micro Bio-Spin 6 columns (Bio-Rad, cat# 732-6221) 50-μL quartz cuvettes (Agilent Technologies, cat# 5062-2496) .. NanoPure water Xho I restriction endonucleases (NEB) Alw NI restriction endonucleases (NEB) Bovine serum albumin (BSA), 20 mg/mL (NEB, cat# B9000S) Ethanol 200 proof TAE buffer: 40 m M Tris, 20 m M acetic acid, 1 m M EDTA, pH 8.0 TE buffer: 10 m M Tris–HCl, 1 m M EDTA, pH 8.0 Agarose, Type I-A, Low-EEO (Sigma cat# A0169) for analytical electrophoresis Agarose, Certified Molecular Biology (Bio-Rad cat# 161-3101) for purification of dsDNA and gapped DNA molecules by electroelution 1-kb DNA ladder Molecular Weight Marker 10 × DNA loading buffer for agarose gels (0.25% bromophenol blue, 50% glycerol, 1 m M EDTA, pH 8.0) Ethidium bromide staining solution, 2 μg/mL in water 1-Butanol ≥99.4% ACS reagent (Sigma cat# 360465) 10 × annealing buffer: 250 m M Tris-acetate, pH 7.5, 500 m M NaCl Formamide ≥99.5% (Sigma cat# F9037) .. Carry out the cleavage of pBS II SK (+) plasmid dsDNA with Xho I and Alw NI restriction endonucleases.

    DNA Extraction:

    Article Title: A distinct subset of Atypical Spitz Tumors is characterized by BRAF mutation and loss of BAP1 expression
    Article Snippet: Paragraph title: DNA extraction and Sanger sequencing ... BSA (New England Biolabs), 1 U Hotstar Taq (Qiagen), 1× Hotstar Taq buffer (Qiagen) and 0.4 µM primer.

    Enzyme Activity Assay:

    Article Title: Identification of Grass-specific Enzyme That Acylates Monolignols with p-Coumarate
    Article Snippet: Authentic coniferyl p -coumarate 3Ga and sinapyl p -coumarate 3Sa were synthesized as described previously ( ). p -Coumaryl p -coumarate 3Ha and sinapyl acetate were made by an analogous route ( ). .. The OsPMT enzyme activity assay, in 50 m m sodium phosphate buffer, pH 6, containing 1 m m dithiothreitol (DTT), 1 m m CoA thioester, 1 m m monolignol, and deionized water to produce a final volume of 50 μl, was initiated by adding of 1 μg of purified PMT protein in 1× BSA (NEB). .. After a 30-min incubation, the reaction was stopped by the addition of 100 m m hydrochloric acid.

    Magnetic Beads:

    Article Title: Evolutionarily Conserved Principles Predict 3D Chromatin Organization
    Article Snippet: This reaction was placed at 37°C for 1.5 h, after which samples were ligated for 4 h at room temperature with addition of 663 µl H2 O, 120 µl 10× NEB T4 DNA Ligase buffer, 100 µl 10% Triton X-100, 12 µl 10 mg/ml BSA, and 5 µl 400 u/µl T4 DNA Ligase (NEB M0202). .. Cell debris was pelleted and the supernatant was transferred into a new 1.5 ml tube for immunoprecipitation.

    Article Title: Staphylococcus aureus Cas9 is a multiple-turnover enzyme
    Article Snippet: Taken together, these findings suggest that SauCas9 may be an attractive alternative or complement to SpyCas9 and could possibly be leveraged for future biotechnological and therapeutic applications. .. S. pyogenes Cas9 (# M0386M), EnGen sgRNA Synthesis Kit, S. pyogenes (# E3322S), HiScribe T7 High Yield RNA Synthesis Kit (E2040S), 2× RNA Loading Dye (# B0363S), Nucleoside Digestion Mix (M0649S), Q5 Hot Start High-Fidelity 2× Master Mix (# M0494L), Monarch PCR & DNA Cleanup Kit (# T1030L), Proteinase K (# P8107S), Shrimp Alkaline Phosphatase (# M0371S), T4 Polynucleotide Kinase (# M0201S), Streptavidin Magnetic Beads (# S1420S), and NEBuffer 3.1 (# B7203S) with a 1× composition of 100 mM NaCl, 50 mM Tris-HCl, 10 mM MgCl2 , 100 μg/ml BSA, pH 7.9 at 25°C were all from New England Biolabs. .. Both S. pyogenes and S. aureus Cas9 were purified at New England Biolabs using standard liquid chromatography protein purification techniques.

    Article Title: YY1 Is a Structural Regulator of Enhancer-Promoter Loops
    Article Snippet: Proximity ligation was performed by addition of 947 μL of ligation master mix, containing 150 μL of 10X NEB T4 DNA ligase buffer (NEB, B0202), 125 μL of 10% Triton X-100, 7.5 μL of 20 mg/mL BSA (NEB, B9000), 10 μL of 400 U/μL T4 DNA ligase (NEB, M0202), and 655.5 μL of water, and incubation at room temperature for 4 hours with rotation. .. Nuclei were sonicated using a Covaris S220 for 6 minutes with the following settings: fill level 8, duty cycle 5, peak incidence power 140, cycles per burst 200.

    Microscopy:

    Article Title: A distinct subset of Atypical Spitz Tumors is characterized by BRAF mutation and loss of BAP1 expression
    Article Snippet: Tumor and non-tumor areas were separately microdissected from sections of archival paraffin-embedded tissue using a dissection microscope. .. BSA (New England Biolabs), 1 U Hotstar Taq (Qiagen), 1× Hotstar Taq buffer (Qiagen) and 0.4 µM primer.

    Purification:

    Article Title: Reconstituting the 4-Strand DNA Strand Exchange
    Article Snippet: Standard agarose gel running setup (Bio-Rad Wide Mini-Sub Cell GT) AlphaImager 3400 gel documentation station (Alpha Innotech) Vortex-2 Genie (Scientific Industries) Low-speed benchtop centrifuge Heat blocks or water baths set at 37°C and 65°C UV lamp Electroelution apparatus: Schleicher & Schuell Elutrap (Whatman) Micro Bio-Spin 6 columns (Bio-Rad, cat# 732-6221) 50-μL quartz cuvettes (Agilent Technologies, cat# 5062-2496) .. NanoPure water Xho I restriction endonucleases (NEB) Alw NI restriction endonucleases (NEB) Bovine serum albumin (BSA), 20 mg/mL (NEB, cat# B9000S) Ethanol 200 proof TAE buffer: 40 m M Tris, 20 m M acetic acid, 1 m M EDTA, pH 8.0 TE buffer: 10 m M Tris–HCl, 1 m M EDTA, pH 8.0 Agarose, Type I-A, Low-EEO (Sigma cat# A0169) for analytical electrophoresis Agarose, Certified Molecular Biology (Bio-Rad cat# 161-3101) for purification of dsDNA and gapped DNA molecules by electroelution 1-kb DNA ladder Molecular Weight Marker 10 × DNA loading buffer for agarose gels (0.25% bromophenol blue, 50% glycerol, 1 m M EDTA, pH 8.0) Ethidium bromide staining solution, 2 μg/mL in water 1-Butanol ≥99.4% ACS reagent (Sigma cat# 360465) 10 × annealing buffer: 250 m M Tris-acetate, pH 7.5, 500 m M NaCl Formamide ≥99.5% (Sigma cat# F9037) .. Carry out the cleavage of pBS II SK (+) plasmid dsDNA with Xho I and Alw NI restriction endonucleases.

    Article Title: A distinct subset of Atypical Spitz Tumors is characterized by BRAF mutation and loss of BAP1 expression
    Article Snippet: DNA was extracted and purified with a QIAamp DNA FFPE Tissue Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions. .. BSA (New England Biolabs), 1 U Hotstar Taq (Qiagen), 1× Hotstar Taq buffer (Qiagen) and 0.4 µM primer.

    Article Title: Specific DNA-binding by Apicomplexan AP2 transcription factors
    Article Snippet: Primer extension from a universal 24-mer region on all 60-mers generates a double-stranded DNA microarray platform. .. Purified proteins were diluted to a final concentration of 100–500 nM in PBS, 2% (wt/vol) milk, 51.3 ng/μl salmon testes DNA (Sigma), 0.2 μg/μl BSA (New England Biolabs), and incubated for 1 h at 20°C. .. After washing, specific DNA–protein interactions were visualized by using a GSI Lumonics ScanArray 5000 scanner to detect fluorescence from an Alexa488-conjugated anti-GST antibody.

    Article Title: Identification of Grass-specific Enzyme That Acylates Monolignols with p-Coumarate
    Article Snippet: Authentic coniferyl p -coumarate 3Ga and sinapyl p -coumarate 3Sa were synthesized as described previously ( ). p -Coumaryl p -coumarate 3Ha and sinapyl acetate were made by an analogous route ( ). .. The OsPMT enzyme activity assay, in 50 m m sodium phosphate buffer, pH 6, containing 1 m m dithiothreitol (DTT), 1 m m CoA thioester, 1 m m monolignol, and deionized water to produce a final volume of 50 μl, was initiated by adding of 1 μg of purified PMT protein in 1× BSA (NEB). .. After a 30-min incubation, the reaction was stopped by the addition of 100 m m hydrochloric acid.

    Sequencing:

    Article Title: A distinct subset of Atypical Spitz Tumors is characterized by BRAF mutation and loss of BAP1 expression
    Article Snippet: Paragraph title: DNA extraction and Sanger sequencing ... BSA (New England Biolabs), 1 U Hotstar Taq (Qiagen), 1× Hotstar Taq buffer (Qiagen) and 0.4 µM primer.

    Article Title: Differential and Cross-Transcriptional Control of Duplicated Genes Encoding Alternative Sigma Factors in Streptomyces ambofaciens
    Article Snippet: All restriction enzymes, PCR reagents, and other molecular biology reagents were purchased from New England Biolabs or Roche Diagnostics. .. All restriction enzymes, PCR reagents, and other molecular biology reagents were purchased from New England Biolabs or Roche Diagnostics.

    Software:

    Article Title: A distinct subset of Atypical Spitz Tumors is characterized by BRAF mutation and loss of BAP1 expression
    Article Snippet: BSA (New England Biolabs), 1 U Hotstar Taq (Qiagen), 1× Hotstar Taq buffer (Qiagen) and 0.4 µM primer. .. BSA (New England Biolabs), 1 U Hotstar Taq (Qiagen), 1× Hotstar Taq buffer (Qiagen) and 0.4 µM primer.

    Article Title: Identification of Grass-specific Enzyme That Acylates Monolignols with p-Coumarate
    Article Snippet: Purified CoA thioesters were analyzed for purity using an Acquity Ultra Performance LC with an Acquity UPLC BEH C18 1.7 μm 2.1 ×100-mm column and the Acquity Console and Empower 2 Software (Waters Corporation). .. The OsPMT enzyme activity assay, in 50 m m sodium phosphate buffer, pH 6, containing 1 m m dithiothreitol (DTT), 1 m m CoA thioester, 1 m m monolignol, and deionized water to produce a final volume of 50 μl, was initiated by adding of 1 μg of purified PMT protein in 1× BSA (NEB).

    Article Title: Differential and Cross-Transcriptional Control of Duplicated Genes Encoding Alternative Sigma Factors in Streptomyces ambofaciens
    Article Snippet: All restriction enzymes, PCR reagents, and other molecular biology reagents were purchased from New England Biolabs or Roche Diagnostics. .. All restriction enzymes, PCR reagents, and other molecular biology reagents were purchased from New England Biolabs or Roche Diagnostics.

    Immunoprecipitation:

    Article Title: Evolutionarily Conserved Principles Predict 3D Chromatin Organization
    Article Snippet: This reaction was placed at 37°C for 1.5 h, after which samples were ligated for 4 h at room temperature with addition of 663 µl H2 O, 120 µl 10× NEB T4 DNA Ligase buffer, 100 µl 10% Triton X-100, 12 µl 10 mg/ml BSA, and 5 µl 400 u/µl T4 DNA Ligase (NEB M0202). .. Following ligation, nuclei were pelleted and resuspended in 200 µl cold Nuclei Lysis Buffer (50 mM Tris-HCl pH 9, 10 mM EDTA, 1% SDS, and 1× Protease Inhibitors) with incubation on ice for 20 min. After incubation we added 100 µl cold IP Dilution Buffer (0.01% SDS, 1.1% Trition X-100, 1.2 mM EDTA, 16.7 Tris-HCl pH 8, 16.7 mM NaCl, and 1× Protease Inhibitors) and sonicated to approximately 250 bp fragments.

    HiChIP:

    Article Title: Evolutionarily Conserved Principles Predict 3D Chromatin Organization
    Article Snippet: Paragraph title: Hi-C, ChIA-PET, and HiChIP Library Preparation and Processing ... This reaction was placed at 37°C for 1.5 h, after which samples were ligated for 4 h at room temperature with addition of 663 µl H2 O, 120 µl 10× NEB T4 DNA Ligase buffer, 100 µl 10% Triton X-100, 12 µl 10 mg/ml BSA, and 5 µl 400 u/µl T4 DNA Ligase (NEB M0202).

    Article Title: YY1 Is a Structural Regulator of Enhancer-Promoter Loops
    Article Snippet: Paragraph title: HiChIP ... Proximity ligation was performed by addition of 947 μL of ligation master mix, containing 150 μL of 10X NEB T4 DNA ligase buffer (NEB, B0202), 125 μL of 10% Triton X-100, 7.5 μL of 20 mg/mL BSA (NEB, B9000), 10 μL of 400 U/μL T4 DNA ligase (NEB, M0202), and 655.5 μL of water, and incubation at room temperature for 4 hours with rotation.

    Lysis:

    Article Title: Editing DNA methylation in the mammalian genome
    Article Snippet: Cell pellet was re-suspended with 550 µl lysis buffer (10 mM Tris-HCl with pH 8.0, 10 mM NaCl, and 0.2% IGEPAL CA630 with proteinase inhibitor), and incubated on ice for 20 min. .. Then 713 µl H2O, 120 µl 10 x T4 DNA ligase buffer (NEB, B0202), 100 µl 10% Triton X-100, 12 µl 10 mg/ml BSA, and 5 µl T4 DNA ligase (NEB, M0202) were added and incubated for 22 hour at 16 °C.

    Article Title: Evolutionarily Conserved Principles Predict 3D Chromatin Organization
    Article Snippet: Cells were pelleted and resuspended in 500 µl cold Hi-C lysis buffer (10 mM Tris-HCl pH8, 10 mM NaCl, 0.2% Igepal CA-630, and 1× Protease Inhibitor (Roche 11873580001) and incubated on ice for 1 h. Nuclei were pelleted at 2500 rcf for 5 min at 4°C, resuspended in 100 µl 0. .. This reaction was placed at 37°C for 1.5 h, after which samples were ligated for 4 h at room temperature with addition of 663 µl H2 O, 120 µl 10× NEB T4 DNA Ligase buffer, 100 µl 10% Triton X-100, 12 µl 10 mg/ml BSA, and 5 µl 400 u/µl T4 DNA Ligase (NEB M0202).

    Article Title: YY1 Is a Structural Regulator of Enhancer-Promoter Loops
    Article Snippet: Nuclei were pelleted by centrifugation at 2500 r cf. for 5 min at 4°C and washed once with 500 μL of ice-cold Hi-C lysis buffer. .. Proximity ligation was performed by addition of 947 μL of ligation master mix, containing 150 μL of 10X NEB T4 DNA ligase buffer (NEB, B0202), 125 μL of 10% Triton X-100, 7.5 μL of 20 mg/mL BSA (NEB, B9000), 10 μL of 400 U/μL T4 DNA ligase (NEB, M0202), and 655.5 μL of water, and incubation at room temperature for 4 hours with rotation.

    IA:

    Article Title: Archaeal JAB1/MPN/MOV34 metalloenzyme (HvJAMM1) cleaves ubiquitin-like small archaeal modifier proteins (SAMPs) from protein-conjugates
    Article Snippet: Molecular biology grade enzymes were purchased from New England Biolabs (Ipswich, MA) unless otherwise indicated. .. Molecular biology grade enzymes were purchased from New England Biolabs (Ipswich, MA) unless otherwise indicated.

    Silver Staining:

    Article Title: Specific DNA-binding by Apicomplexan AP2 transcription factors
    Article Snippet: Purity was verified by silver stain SDS/PAGE and Western blot analysis with an anti-GST antibody (Invitrogen). .. Purified proteins were diluted to a final concentration of 100–500 nM in PBS, 2% (wt/vol) milk, 51.3 ng/μl salmon testes DNA (Sigma), 0.2 μg/μl BSA (New England Biolabs), and incubated for 1 h at 20°C.

    Liquid Chromatography:

    Article Title: Identification of Grass-specific Enzyme That Acylates Monolignols with p-Coumarate
    Article Snippet: Purified CoA thioesters were analyzed for purity using an Acquity Ultra Performance LC with an Acquity UPLC BEH C18 1.7 μm 2.1 ×100-mm column and the Acquity Console and Empower 2 Software (Waters Corporation). .. The OsPMT enzyme activity assay, in 50 m m sodium phosphate buffer, pH 6, containing 1 m m dithiothreitol (DTT), 1 m m CoA thioester, 1 m m monolignol, and deionized water to produce a final volume of 50 μl, was initiated by adding of 1 μg of purified PMT protein in 1× BSA (NEB).

    Plasmid Preparation:

    Article Title: A solenoid design for assessing determinants of parallel β-sheet registration
    Article Snippet: Molecular biology reagents were purchased from New England Biolabs. .. Molecular biology reagents were purchased from New England Biolabs.

    Article Title: Differential and Cross-Transcriptional Control of Duplicated Genes Encoding Alternative Sigma Factors in Streptomyces ambofaciens
    Article Snippet: Cosmid and plasmid DNAs were extracted from E. coli by the alkaline method ( ). .. All restriction enzymes, PCR reagents, and other molecular biology reagents were purchased from New England Biolabs or Roche Diagnostics.

    Article Title: Common and Distinguishing Regulatory and Expression Characteristics of the Highly Related KorB Proteins of Streptomycete Plasmids pIJ101 and pSB24.2
    Article Snippet: Ampicillin was included at 50 μg/ml when plasmid-containing E. coli strains were being grown ( ); when Streptomyces strains containing resistance-encoding plasmids were being grown, thiostrepton was included at a concentration of 50 or 5 μg/ml in solid or liquid media, respectively, and hygromycin was included at a concentration of 200 or 20 μg/ml in solid or liquid media, respectively. .. Standard molecular biology protocols ( ) and enzymes purchased from New England BioLabs or Invitrogen were used to construct various pGSP and pSCON plasmids listed in Table .

    TaqMan Assay:

    Article Title: Association between two ?-opioid receptor gene (OPRM1) haplotype blocks and drug or alcohol dependence
    Article Snippet: All other SNPs were genotyped using the TaqMan assay in a 384-well microplate format. .. Briefly, 1 ng of DNA was amplified in a final volume of 2 μl containing 0.05 μl of 20× (or 0.025 μl of 40×) MGB probes and primers, 1 μl of 2× TaqMan Universal PCR Master Mix, and 0.004 μl of 100× BSA (New England Biolabs Inc., Beverly, MA).

    Electrophoresis:

    Article Title: Reconstituting the 4-Strand DNA Strand Exchange
    Article Snippet: Standard agarose gel running setup (Bio-Rad Wide Mini-Sub Cell GT) AlphaImager 3400 gel documentation station (Alpha Innotech) Vortex-2 Genie (Scientific Industries) Low-speed benchtop centrifuge Heat blocks or water baths set at 37°C and 65°C UV lamp Electroelution apparatus: Schleicher & Schuell Elutrap (Whatman) Micro Bio-Spin 6 columns (Bio-Rad, cat# 732-6221) 50-μL quartz cuvettes (Agilent Technologies, cat# 5062-2496) .. NanoPure water Xho I restriction endonucleases (NEB) Alw NI restriction endonucleases (NEB) Bovine serum albumin (BSA), 20 mg/mL (NEB, cat# B9000S) Ethanol 200 proof TAE buffer: 40 m M Tris, 20 m M acetic acid, 1 m M EDTA, pH 8.0 TE buffer: 10 m M Tris–HCl, 1 m M EDTA, pH 8.0 Agarose, Type I-A, Low-EEO (Sigma cat# A0169) for analytical electrophoresis Agarose, Certified Molecular Biology (Bio-Rad cat# 161-3101) for purification of dsDNA and gapped DNA molecules by electroelution 1-kb DNA ladder Molecular Weight Marker 10 × DNA loading buffer for agarose gels (0.25% bromophenol blue, 50% glycerol, 1 m M EDTA, pH 8.0) Ethidium bromide staining solution, 2 μg/mL in water 1-Butanol ≥99.4% ACS reagent (Sigma cat# 360465) 10 × annealing buffer: 250 m M Tris-acetate, pH 7.5, 500 m M NaCl Formamide ≥99.5% (Sigma cat# F9037) .. Carry out the cleavage of pBS II SK (+) plasmid dsDNA with Xho I and Alw NI restriction endonucleases.

    Article Title: Direct contacts between conserved motifs of different subunits provide major contribution to active site organization in human and mycobacterial dUTPases
    Article Snippet: To provide novel insights into the mechanism of this important and unique enzyme, with potential focus on the role of the metal co-factor, we decided to investigate the contribution of Motif I aspartate to structure and function. .. Materials for electrophoresis or chromatography were from Bio-Rad or Amersham Biosciences, phenol red from Merck, molecular biology products were from New England BioLabs or Fermentas. .. Other materials were from Sigma or Stratagene.

    Recombinant:

    Article Title: Engineering yeast for the production of breviscapine by genomic analysis and synthetic biology approaches
    Article Snippet: T4 ligase, Bsa I, and 100 × bovine serum albumin (BSA) were purchased from NEB, USA. .. T4 ligase, Bsa I, and 100 × bovine serum albumin (BSA) were purchased from NEB, USA.

    In Situ:

    Article Title: Quantification and localization of oncogenic receptor tyrosine kinase variant transcripts using molecular inversion probes
    Article Snippet: In situ reverse transcription was performed using BNA primers positioned within ≤1 nt from the 5′ end of the padlock probe target site (see Table for nucleotide sequences). .. BNA primer hybridization and mRNA reverse transcription were performed o/n at 42 °C using 200 nM of BNA primer (IDT, Leuven Belgium), 20 U/µl RevertAid H minus M-MuLV reverse transcriptase in 1x M-MuLV reaction buffer (ThermoFisher Scientific, Waltham, MA, USA), 1 U/µl RiboLock RNase Inhibitor (ThermoFisher Scientific, Waltham, MA, USA), 500 µM dNTP and 0.2 µg/µl BSA (New England Biolabs, Ipswich, MA, USA).

    Ethanol Precipitation:

    Article Title: Secondary structure and DNA binding by the C-terminal domain of the transcriptional activator NifA from Klebsiella pneumoniae
    Article Snippet: Reactions for the binding assays contained 5 nM DNA, 8.1 ng/µl poly(dI–dC) (Boehringer Mannheim), 25 mM Tris acetate pH 8, 50 mM potassium acetate, 13.5 mM ammonium acetate, 0.5 µM acetylated BSA (NEB) and 2.5–7.5 µM NifA C-terminal DNA-binding domain in a final volume of 15 µl. .. Reactions for the binding assays contained 5 nM DNA, 8.1 ng/µl poly(dI–dC) (Boehringer Mannheim), 25 mM Tris acetate pH 8, 50 mM potassium acetate, 13.5 mM ammonium acetate, 0.5 µM acetylated BSA (NEB) and 2.5–7.5 µM NifA C-terminal DNA-binding domain in a final volume of 15 µl.

    Concentration Assay:

    Article Title: Specific DNA-binding by Apicomplexan AP2 transcription factors
    Article Snippet: Primer extension from a universal 24-mer region on all 60-mers generates a double-stranded DNA microarray platform. .. Purified proteins were diluted to a final concentration of 100–500 nM in PBS, 2% (wt/vol) milk, 51.3 ng/μl salmon testes DNA (Sigma), 0.2 μg/μl BSA (New England Biolabs), and incubated for 1 h at 20°C. .. After washing, specific DNA–protein interactions were visualized by using a GSI Lumonics ScanArray 5000 scanner to detect fluorescence from an Alexa488-conjugated anti-GST antibody.

    Article Title: Identification of Grass-specific Enzyme That Acylates Monolignols with p-Coumarate
    Article Snippet: The concentration for each CoA thioester was calculated based on its absorbance maximum and extinction coefficient ( , ). .. The OsPMT enzyme activity assay, in 50 m m sodium phosphate buffer, pH 6, containing 1 m m dithiothreitol (DTT), 1 m m CoA thioester, 1 m m monolignol, and deionized water to produce a final volume of 50 μl, was initiated by adding of 1 μg of purified PMT protein in 1× BSA (NEB).

    Article Title: Staphylococcus aureus Cas9 is a multiple-turnover enzyme
    Article Snippet: S. pyogenes Cas9 (# M0386M), EnGen sgRNA Synthesis Kit, S. pyogenes (# E3322S), HiScribe T7 High Yield RNA Synthesis Kit (E2040S), 2× RNA Loading Dye (# B0363S), Nucleoside Digestion Mix (M0649S), Q5 Hot Start High-Fidelity 2× Master Mix (# M0494L), Monarch PCR & DNA Cleanup Kit (# T1030L), Proteinase K (# P8107S), Shrimp Alkaline Phosphatase (# M0371S), T4 Polynucleotide Kinase (# M0201S), Streptavidin Magnetic Beads (# S1420S), and NEBuffer 3.1 (# B7203S) with a 1× composition of 100 mM NaCl, 50 mM Tris-HCl, 10 mM MgCl2 , 100 μg/ml BSA, pH 7.9 at 25°C were all from New England Biolabs. .. Both S. pyogenes and S. aureus Cas9 were purified at New England Biolabs using standard liquid chromatography protein purification techniques.

    Article Title: Common and Distinguishing Regulatory and Expression Characteristics of the Highly Related KorB Proteins of Streptomycete Plasmids pIJ101 and pSB24.2
    Article Snippet: Ampicillin was included at 50 μg/ml when plasmid-containing E. coli strains were being grown ( ); when Streptomyces strains containing resistance-encoding plasmids were being grown, thiostrepton was included at a concentration of 50 or 5 μg/ml in solid or liquid media, respectively, and hygromycin was included at a concentration of 200 or 20 μg/ml in solid or liquid media, respectively. .. Standard molecular biology protocols ( ) and enzymes purchased from New England BioLabs or Invitrogen were used to construct various pGSP and pSCON plasmids listed in Table .

    Pulsed-Field Gel:

    Article Title: Differential and Cross-Transcriptional Control of Duplicated Genes Encoding Alternative Sigma Factors in Streptomyces ambofaciens
    Article Snippet: Standard or pulsed-field gel electrophoresis DNA preparations from S. ambofaciens were made as described earlier ( , ). .. All restriction enzymes, PCR reagents, and other molecular biology reagents were purchased from New England Biolabs or Roche Diagnostics.

    Marker:

    Article Title: Reconstituting the 4-Strand DNA Strand Exchange
    Article Snippet: Standard agarose gel running setup (Bio-Rad Wide Mini-Sub Cell GT) AlphaImager 3400 gel documentation station (Alpha Innotech) Vortex-2 Genie (Scientific Industries) Low-speed benchtop centrifuge Heat blocks or water baths set at 37°C and 65°C UV lamp Electroelution apparatus: Schleicher & Schuell Elutrap (Whatman) Micro Bio-Spin 6 columns (Bio-Rad, cat# 732-6221) 50-μL quartz cuvettes (Agilent Technologies, cat# 5062-2496) .. NanoPure water Xho I restriction endonucleases (NEB) Alw NI restriction endonucleases (NEB) Bovine serum albumin (BSA), 20 mg/mL (NEB, cat# B9000S) Ethanol 200 proof TAE buffer: 40 m M Tris, 20 m M acetic acid, 1 m M EDTA, pH 8.0 TE buffer: 10 m M Tris–HCl, 1 m M EDTA, pH 8.0 Agarose, Type I-A, Low-EEO (Sigma cat# A0169) for analytical electrophoresis Agarose, Certified Molecular Biology (Bio-Rad cat# 161-3101) for purification of dsDNA and gapped DNA molecules by electroelution 1-kb DNA ladder Molecular Weight Marker 10 × DNA loading buffer for agarose gels (0.25% bromophenol blue, 50% glycerol, 1 m M EDTA, pH 8.0) Ethidium bromide staining solution, 2 μg/mL in water 1-Butanol ≥99.4% ACS reagent (Sigma cat# 360465) 10 × annealing buffer: 250 m M Tris-acetate, pH 7.5, 500 m M NaCl Formamide ≥99.5% (Sigma cat# F9037) .. Carry out the cleavage of pBS II SK (+) plasmid dsDNA with Xho I and Alw NI restriction endonucleases.

    Staining:

    Article Title: Reconstituting the 4-Strand DNA Strand Exchange
    Article Snippet: Standard agarose gel running setup (Bio-Rad Wide Mini-Sub Cell GT) AlphaImager 3400 gel documentation station (Alpha Innotech) Vortex-2 Genie (Scientific Industries) Low-speed benchtop centrifuge Heat blocks or water baths set at 37°C and 65°C UV lamp Electroelution apparatus: Schleicher & Schuell Elutrap (Whatman) Micro Bio-Spin 6 columns (Bio-Rad, cat# 732-6221) 50-μL quartz cuvettes (Agilent Technologies, cat# 5062-2496) .. NanoPure water Xho I restriction endonucleases (NEB) Alw NI restriction endonucleases (NEB) Bovine serum albumin (BSA), 20 mg/mL (NEB, cat# B9000S) Ethanol 200 proof TAE buffer: 40 m M Tris, 20 m M acetic acid, 1 m M EDTA, pH 8.0 TE buffer: 10 m M Tris–HCl, 1 m M EDTA, pH 8.0 Agarose, Type I-A, Low-EEO (Sigma cat# A0169) for analytical electrophoresis Agarose, Certified Molecular Biology (Bio-Rad cat# 161-3101) for purification of dsDNA and gapped DNA molecules by electroelution 1-kb DNA ladder Molecular Weight Marker 10 × DNA loading buffer for agarose gels (0.25% bromophenol blue, 50% glycerol, 1 m M EDTA, pH 8.0) Ethidium bromide staining solution, 2 μg/mL in water 1-Butanol ≥99.4% ACS reagent (Sigma cat# 360465) 10 × annealing buffer: 250 m M Tris-acetate, pH 7.5, 500 m M NaCl Formamide ≥99.5% (Sigma cat# F9037) .. Carry out the cleavage of pBS II SK (+) plasmid dsDNA with Xho I and Alw NI restriction endonucleases.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars

  • 81
    New England Biolabs bovine serum albumen
    Bovine Serum Albumen, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 81/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bovine serum albumen/product/New England Biolabs
    Average 81 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bovine serum albumen - by Bioz Stars, 2019-12
    81/100 stars
      Buy from Supplier

    Image Search Results