bsa  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97

    Structured Review

    New England Biolabs bsa
    Bsa, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bsa/product/New England Biolabs
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bsa - by Bioz Stars, 2022-07
    97/100 stars

    Images

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97
    New England Biolabs bsa molecular biology grade
    Electrophoresis mobility shift assay (EMSA) and DNA footprinting assays indicate <t>HlyU</t> binding to a region within the exsA promoter. (A) Six percent TBE-polyacrylamide gels were loaded with reaction mixtures containing exsA promoter DNA and increasing amounts of purified HlyU-His or bovine serum albumin <t>(BSA).</t> An exsA promoter DNA-only control was included as indicated. The white box indicates slow-migrating, concentrated DNA species. (B) A 6% TBE-polyacrylamide gel was loaded with reaction mixtures containing nleH1 DNA and increasing amounts of purified HlyU-His. Following electrophoresis, gels were stained with SYBR green to specifically stain DNA. (C) A DNase I footprinting assay using purified HlyU-His was used to identify a DNA binding region for HlyU-His within the exsA promoter. The approximate base pair numbers of the HlyU-His footprint region are indicated (259 to 315 bp) and are based on capillary electrophoresis internal size standards. The DNA sequence associated with the identified HlyU protected region is displayed below the chromatogram. A 7-bp inverted repeat (labeled in blue) and separated by 14 bp is centrally located within the HlyU-His protected region.
    Bsa Molecular Biology Grade, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bsa molecular biology grade/product/New England Biolabs
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bsa molecular biology grade - by Bioz Stars, 2022-07
    97/100 stars
      Buy from Supplier

    Image Search Results


    Electrophoresis mobility shift assay (EMSA) and DNA footprinting assays indicate HlyU binding to a region within the exsA promoter. (A) Six percent TBE-polyacrylamide gels were loaded with reaction mixtures containing exsA promoter DNA and increasing amounts of purified HlyU-His or bovine serum albumin (BSA). An exsA promoter DNA-only control was included as indicated. The white box indicates slow-migrating, concentrated DNA species. (B) A 6% TBE-polyacrylamide gel was loaded with reaction mixtures containing nleH1 DNA and increasing amounts of purified HlyU-His. Following electrophoresis, gels were stained with SYBR green to specifically stain DNA. (C) A DNase I footprinting assay using purified HlyU-His was used to identify a DNA binding region for HlyU-His within the exsA promoter. The approximate base pair numbers of the HlyU-His footprint region are indicated (259 to 315 bp) and are based on capillary electrophoresis internal size standards. The DNA sequence associated with the identified HlyU protected region is displayed below the chromatogram. A 7-bp inverted repeat (labeled in blue) and separated by 14 bp is centrally located within the HlyU-His protected region.

    Journal: Journal of Bacteriology

    Article Title: The Transcriptional Regulator HlyU Positively Regulates Expression of exsA, Leading to Type III Secretion System 1 Activation in Vibrio parahaemolyticus

    doi: 10.1128/JB.00653-17

    Figure Lengend Snippet: Electrophoresis mobility shift assay (EMSA) and DNA footprinting assays indicate HlyU binding to a region within the exsA promoter. (A) Six percent TBE-polyacrylamide gels were loaded with reaction mixtures containing exsA promoter DNA and increasing amounts of purified HlyU-His or bovine serum albumin (BSA). An exsA promoter DNA-only control was included as indicated. The white box indicates slow-migrating, concentrated DNA species. (B) A 6% TBE-polyacrylamide gel was loaded with reaction mixtures containing nleH1 DNA and increasing amounts of purified HlyU-His. Following electrophoresis, gels were stained with SYBR green to specifically stain DNA. (C) A DNase I footprinting assay using purified HlyU-His was used to identify a DNA binding region for HlyU-His within the exsA promoter. The approximate base pair numbers of the HlyU-His footprint region are indicated (259 to 315 bp) and are based on capillary electrophoresis internal size standards. The DNA sequence associated with the identified HlyU protected region is displayed below the chromatogram. A 7-bp inverted repeat (labeled in blue) and separated by 14 bp is centrally located within the HlyU-His protected region.

    Article Snippet: Purified HlyU-His or BSA (B9000S; New England BioLabs) was mixed with a PCR-amplified exsA promoter DNA fragment in binding buffer (10 mM Tris [pH 7.5 at 20°C], 1 mM EDTA, 0.1 M KCl, 0.1 mM dithiothreitol, 5%, vol/vol, glycerol, 0.01 mg ml−1 BSA).

    Techniques: Electrophoresis, Mobility Shift, DNA Footprinting, Binding Assay, Purification, Staining, SYBR Green Assay, Footprinting, Sequencing, Labeling

    Enzymatic properties of recombinant hENDOV proteins. (A) RNA cleavage activity of hENDOV 282 (282), hENDOV 308 (308) and hENDOV 309 (309) enzymes. Increasing amounts of enzyme (20, 40, 80 and 160 fmol) were incubated with a single stranded (ss; upper) or double-stranded (ds; lower) RNA substrates containing inosine and reaction products were analyzed by denaturing gel electrophoresis. Quantification of the cleavage activity by ImageQuant TL software (n = 3) is shown below. (B) Electrophoretic mobility shift assay using hENDOV 282 (282), hENDOV 308 (308) and hENDOV 309 (309) enzymes (1, 2 or 3 pmol) and ss (left panel) or ds (right panel) RNA substrates containing inosine. Reactions were incubated on ice prior to separation of enzyme-substrate complexes from unbound substrate on native polyacrylamide gels. Quantification of band shifts by ImageQuant TL software (n = 3) is shown below. (C) Representative images of northern blot analyses of total RNA from HAP1 cells (WT) incubated with recombinant hENDOV 282 (282), hENDOV 308 (308) and hENDOV 309 (309) enzymes (75 and 150 pmol). The probes used recognize AlaAGC5’, ArgACG5’ or ValAAC5’ tRNAs. Sample without enzyme (-) or with 150 pmol BSA were included as controls. Equal loading is shown by ethidium bromide (Eth. Br.) staining of the gel (lower panel). Glyphs to the right of the membranes indicate full-length and fragmented tRNA species.

    Journal: PLoS ONE

    Article Title: Complex alternative splicing of human Endonuclease V mRNA, but evidence for only a single protein isoform

    doi: 10.1371/journal.pone.0225081

    Figure Lengend Snippet: Enzymatic properties of recombinant hENDOV proteins. (A) RNA cleavage activity of hENDOV 282 (282), hENDOV 308 (308) and hENDOV 309 (309) enzymes. Increasing amounts of enzyme (20, 40, 80 and 160 fmol) were incubated with a single stranded (ss; upper) or double-stranded (ds; lower) RNA substrates containing inosine and reaction products were analyzed by denaturing gel electrophoresis. Quantification of the cleavage activity by ImageQuant TL software (n = 3) is shown below. (B) Electrophoretic mobility shift assay using hENDOV 282 (282), hENDOV 308 (308) and hENDOV 309 (309) enzymes (1, 2 or 3 pmol) and ss (left panel) or ds (right panel) RNA substrates containing inosine. Reactions were incubated on ice prior to separation of enzyme-substrate complexes from unbound substrate on native polyacrylamide gels. Quantification of band shifts by ImageQuant TL software (n = 3) is shown below. (C) Representative images of northern blot analyses of total RNA from HAP1 cells (WT) incubated with recombinant hENDOV 282 (282), hENDOV 308 (308) and hENDOV 309 (309) enzymes (75 and 150 pmol). The probes used recognize AlaAGC5’, ArgACG5’ or ValAAC5’ tRNAs. Sample without enzyme (-) or with 150 pmol BSA were included as controls. Equal loading is shown by ethidium bromide (Eth. Br.) staining of the gel (lower panel). Glyphs to the right of the membranes indicate full-length and fragmented tRNA species.

    Article Snippet: A sample with only RNA (no enzyme) and another sample with 150 pmol BSA (Bionordika/NEB, B9000S) were included as controls.

    Techniques: Recombinant, Activity Assay, Incubation, Nucleic Acid Electrophoresis, Software, Electrophoretic Mobility Shift Assay, Northern Blot, Staining