nuclease free water  (New England Biolabs)


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  • 99
    Name:
    Nuclease free Water
    Description:
    Nuclease free Water 100 ml
    Catalog Number:
    b1500l
    Price:
    62
    Size:
    100 ml
    Category:
    Ribonuclease Protection Assays
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    Structured Review

    New England Biolabs nuclease free water
    Nuclease free Water
    Nuclease free Water 100 ml
    https://www.bioz.com/result/nuclease free water/product/New England Biolabs
    Average 99 stars, based on 69 article reviews
    Price from $9.99 to $1999.99
    nuclease free water - by Bioz Stars, 2020-05
    99/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Combination of microbiome analysis and serodiagnostics to assess the risk of pathogen transmission by ticks to humans and animals in central Germany
    Article Snippet: .. Each PCR reaction was performed in a total of 50 μl of reaction solution comrpising 14 μl of nuclease-free water, 10 ng of input DNA, 1 μl of barcode and 25 μl of LongAmp Taq 2X Mastermix (New England Biolabs, Frankfurt, Germany). .. The following PCR protocol was performed: 95 °C for 1 min; 25 cycles at 95 °C for 20 s, 55 °C for 30 s and 65 °C for 2 min, and a final extension step at 72 °C for 5 min. PCR products were purified using 30 μl of AMPure XP DNA beads (Beckman Coulter).

    Article Title: Early MinION™ nanopore single-molecule sequencing technology enables the characterization of hepatitis B virus genetic complexity in clinical samples
    Article Snippet: .. Briefly, the protocol for preparing a nanopore sequencing with SQK-MAP004 is as follows: 500 ng to 1μg of PCR products or cloned HBV DNA were diluted to 80μL using nuclease-free water and end-repaired using the NEBNext End Repair Module (New England Biolabs, Evry, France). .. The end-repaired products were then purified using 1.0 x volume of AMPure XP beads (Beckman-Coulter) and eluted in 25μL of nuclease-free.

    Article Title: RNA Framework: an all-in-one toolkit for the analysis of RNA structures and post-transcriptional modifications
    Article Snippet: .. Template RNA was degraded by adding 1 μl of 5 M NaOH and incubating at 95°C for 3 min. After reaction cleanup, cDNA was eluted in 20 μl nuclease-free water, and barcodes were introduced by 15 cycles of PCR in the presence of 25 pmol of each primer, and 25 μl NEBNext® High-Fidelity 2× PCR Master Mix. .. DMS was diluted 1:6 in 100% ethanol to a final concentration of 1.76 M. Diluted DMS was added to bacteria to a final concentration of ∼105 mM.

    Clone Assay:

    Article Title: Early MinION™ nanopore single-molecule sequencing technology enables the characterization of hepatitis B virus genetic complexity in clinical samples
    Article Snippet: .. Briefly, the protocol for preparing a nanopore sequencing with SQK-MAP004 is as follows: 500 ng to 1μg of PCR products or cloned HBV DNA were diluted to 80μL using nuclease-free water and end-repaired using the NEBNext End Repair Module (New England Biolabs, Evry, France). .. The end-repaired products were then purified using 1.0 x volume of AMPure XP beads (Beckman-Coulter) and eluted in 25μL of nuclease-free.

    Incubation:

    Article Title: MinION nanopore sequencing of an influenza genome
    Article Snippet: .. 8 μL nuclease-free water was added to 30 μL of the dA-tailed DNA, and then 10 μL adapter mix, 2 μL HP adapter and 50 μL Blunt/TA DNA ligase mastermix (New England Biolabs) were added in this order, and then incubated at 25°C for 10 min. .. The ligated DNA was purified using His-tag Dynabeads.

    Flow Cytometry:

    Article Title: Gene Expression-Based Identification of Antigen-Responsive CD8+ T Cells on a Single-Cell Level
    Article Snippet: .. Single cells were flow-sorted into 96-well plates containing 2 μL of nuclease-free water with 0.2% Triton X-100 and 4 U murine RNase inhibitor (NEB), centrifuged, and frozen at −80°C. .. After thawing, 2 μL of the primer mix [5 mM dNTP (Invitrogen), 0.5 μM oligo-dT primer, 4 U murine RNase inhibitor] was added to each well.

    Nanopore Sequencing:

    Article Title: Early MinION™ nanopore single-molecule sequencing technology enables the characterization of hepatitis B virus genetic complexity in clinical samples
    Article Snippet: .. Briefly, the protocol for preparing a nanopore sequencing with SQK-MAP004 is as follows: 500 ng to 1μg of PCR products or cloned HBV DNA were diluted to 80μL using nuclease-free water and end-repaired using the NEBNext End Repair Module (New England Biolabs, Evry, France). .. The end-repaired products were then purified using 1.0 x volume of AMPure XP beads (Beckman-Coulter) and eluted in 25μL of nuclease-free.

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  • 99
    New England Biolabs nuclease free water
    Nuclease Free Water, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nuclease free water/product/New England Biolabs
    Average 99 stars, based on 67 article reviews
    Price from $9.99 to $1999.99
    nuclease free water - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

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