antarctic phosphatase reaction buffer  (New England Biolabs)


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    Name:
    Antarctic Phosphatase Reaction Buffer
    Description:
    Antarctic Phosphatase Reaction Buffer 6 0 ml
    Catalog Number:
    b0289s
    Price:
    24
    Size:
    6 0 ml
    Category:
    Buffers
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    New England Biolabs antarctic phosphatase reaction buffer
    Antarctic Phosphatase Reaction Buffer
    Antarctic Phosphatase Reaction Buffer 6 0 ml
    https://www.bioz.com/result/antarctic phosphatase reaction buffer/product/New England Biolabs
    Average 94 stars, based on 52 article reviews
    Price from $9.99 to $1999.99
    antarctic phosphatase reaction buffer - by Bioz Stars, 2020-09
    94/100 stars

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    Related Articles

    Activation Assay:

    Article Title: Flexible linkers in CaMKII control the balance between activating and inhibitory autophosphorylation
    Article Snippet: .. A phosphatase buffer that contains 1 mM MnCl2 and 400-800 units of λ-phosphatase or 800 units of PP1α was added to the flow chambers displaying activated CaMKII, after the activation buffer has been washed out. .. 1 unit of PP1α is defined as the amount of enzyme that releases 1 nmol phosphate per minute from the phosphorylated substrate DiFMUP (6,8-difluoro-4-methylumbelliferyl phosphate) (EMD Millipore).

    Ethanol Precipitation:

    Article Title: Mapping 3D genome organization relative to nuclear compartments using TSA-Seq as a cytological ruler
    Article Snippet: .. Lamin B1 DamID DamID of lamin B1 was performed in K562 cells as described ( ; ) except that before the DpnI digestion, 1.5 µg genomic DNA was treated for 1 h with 5 U Antarctic phosphatase (M0289S; NEB) in 20 µl 1× Antarctic phosphatase reaction buffer (NEB) followed by ethanol precipitation. .. Lamin B1 DamIDDamID of lamin B1 was performed in K562 cells as described ( ; ) except that before the DpnI digestion, 1.5 µg genomic DNA was treated for 1 h with 5 U Antarctic phosphatase (M0289S; NEB) in 20 µl 1× Antarctic phosphatase reaction buffer (NEB) followed by ethanol precipitation.

    Concentration Assay:

    Article Title: Transcriptome maps of general eukaryotic RNA degradation factors
    Article Snippet: .. The dephosphorylation reaction was performed in antarctic phosphatase reaction buffer (NEB, Germany) supplemented with 1 U/µL of antarctic phosphatase and 1 U/µL of RNase OUT (Invitrogen) at 37°C for 30 min. For rephosphyorylation, beads were incubated in T4 PNK reaction buffer A (Invitrogen) with a final concentration of 1 U/µL T4 PNK, 1 U/µL RNase OUT and 1 mM ATP for 1 hr at 37°C. .. 3´ adapter ligation was performed in T4 RNA ligase buffer (NEB) with 10 U/µL T4 RNA ligase 2 (KQ) (NEB), 10 μM 3′ adapter (5′ 5rApp-TGGAATTCTCGGGTGCCAAGG-3ddC 3′ (IDT), 1 U/µL RNase OUT, and 15% (w/v) PEG 8000 overnight at 16°C.

    Incubation:

    Article Title: mRNA Transfection of Mouse and Human Neural Stem Cell Cultures
    Article Snippet: .. To remove 5′ triphosphate moieties from uncapped transcripts, 10 µl of Antarctic Phosphatase reaction buffer and 5 µl (25U) of Antarctic Phosphatase (New England BioLabs) was added to each RNA product and incubated for 30-minutes at 37°C. .. Synthesized RNA products were then repurified using RNeasy Mini Columns (Qiagen) and quantitated by spectrophotometry and denaturing gel electrophoresis.

    Article Title: Transcriptome maps of general eukaryotic RNA degradation factors
    Article Snippet: .. The dephosphorylation reaction was performed in antarctic phosphatase reaction buffer (NEB, Germany) supplemented with 1 U/µL of antarctic phosphatase and 1 U/µL of RNase OUT (Invitrogen) at 37°C for 30 min. For rephosphyorylation, beads were incubated in T4 PNK reaction buffer A (Invitrogen) with a final concentration of 1 U/µL T4 PNK, 1 U/µL RNase OUT and 1 mM ATP for 1 hr at 37°C. .. 3´ adapter ligation was performed in T4 RNA ligase buffer (NEB) with 10 U/µL T4 RNA ligase 2 (KQ) (NEB), 10 μM 3′ adapter (5′ 5rApp-TGGAATTCTCGGGTGCCAAGG-3ddC 3′ (IDT), 1 U/µL RNase OUT, and 15% (w/v) PEG 8000 overnight at 16°C.

    Article Title: RNA-dependent chromatin association of transcription elongation factors and Pol II CTD kinases
    Article Snippet: .. For dephosphorylation, 1× antarctic phosphatase reaction buffer (NEB, Germany) with 1 U/µL of antarctic phosphatase and 1 U/µL of RNase OUT (Invitrogen) were added and the suspension was incubated at 37°C for 30 min and 800 rpm. ..

    De-Phosphorylation Assay:

    Article Title: Transcriptome maps of general eukaryotic RNA degradation factors
    Article Snippet: .. The dephosphorylation reaction was performed in antarctic phosphatase reaction buffer (NEB, Germany) supplemented with 1 U/µL of antarctic phosphatase and 1 U/µL of RNase OUT (Invitrogen) at 37°C for 30 min. For rephosphyorylation, beads were incubated in T4 PNK reaction buffer A (Invitrogen) with a final concentration of 1 U/µL T4 PNK, 1 U/µL RNase OUT and 1 mM ATP for 1 hr at 37°C. .. 3´ adapter ligation was performed in T4 RNA ligase buffer (NEB) with 10 U/µL T4 RNA ligase 2 (KQ) (NEB), 10 μM 3′ adapter (5′ 5rApp-TGGAATTCTCGGGTGCCAAGG-3ddC 3′ (IDT), 1 U/µL RNase OUT, and 15% (w/v) PEG 8000 overnight at 16°C.

    Article Title: RNA-dependent chromatin association of transcription elongation factors and Pol II CTD kinases
    Article Snippet: .. For dephosphorylation, 1× antarctic phosphatase reaction buffer (NEB, Germany) with 1 U/µL of antarctic phosphatase and 1 U/µL of RNase OUT (Invitrogen) were added and the suspension was incubated at 37°C for 30 min and 800 rpm. ..

    Article Title: Flexible linkers in CaMKII control the balance between activating and inhibitory autophosphorylation
    Article Snippet: .. The dephosphorylation reactions were carried out for defined periods of time (3, 15, or 30 min), following which the phosphatase buffer was washed away with 2 mL DPBS and the autophosphorylation states were examined using the immunofluorescence assay described above. .. Phosphatase assay in solution The HEK 293T cell lysate was diluted by 1:100-200.

    Immunofluorescence:

    Article Title: Flexible linkers in CaMKII control the balance between activating and inhibitory autophosphorylation
    Article Snippet: .. The dephosphorylation reactions were carried out for defined periods of time (3, 15, or 30 min), following which the phosphatase buffer was washed away with 2 mL DPBS and the autophosphorylation states were examined using the immunofluorescence assay described above. .. Phosphatase assay in solution The HEK 293T cell lysate was diluted by 1:100-200.

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  • 94
    New England Biolabs antarctic phosphatase reaction buffer
    Antarctic Phosphatase Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antarctic phosphatase reaction buffer/product/New England Biolabs
    Average 94 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    antarctic phosphatase reaction buffer - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

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