5 bromo 4 chloro 3 indoylphosphate nitroblue tetrazolium bcip nbt color development substrate  (Gold Biotechnology Inc)


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    Gold Biotechnology Inc 5 bromo 4 chloro 3 indoylphosphate nitroblue tetrazolium bcip nbt color development substrate
    5 Bromo 4 Chloro 3 Indoylphosphate Nitroblue Tetrazolium Bcip Nbt Color Development Substrate, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/5 bromo 4 chloro 3 indoylphosphate nitroblue tetrazolium bcip nbt color development substrate/product/Gold Biotechnology Inc
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    5 bromo 4 chloro 3 indoylphosphate nitroblue tetrazolium bcip nbt color development substrate - by Bioz Stars, 2022-12
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    Gold Biotechnology Inc hygromycin solution
    Hygromycin Solution, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Gold Biotechnology Inc 5 bromo 4 chloro 3 indoylphosphate nitroblue tetrazolium bcip nbt color development substrate
    5 Bromo 4 Chloro 3 Indoylphosphate Nitroblue Tetrazolium Bcip Nbt Color Development Substrate, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/5 bromo 4 chloro 3 indoylphosphate nitroblue tetrazolium bcip nbt color development substrate/product/Gold Biotechnology Inc
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    5 bromo 4 chloro 3 indoylphosphate nitroblue tetrazolium bcip nbt color development substrate - by Bioz Stars, 2022-12
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    Gold Biotechnology Inc hygromycin b
    A one-vector CRISPR system for gene disruption in N. oceanica . (A) The pNOC-CRISPR vector series includes a <t>Hygromycin</t> B resistance cassette (HygC, green). A bidirectional promoter (Ribi) drives the transcription of the Cas9-reporter fusion and gRNA scaffold (scaffold, blue), along with the LDSP and CS terminators (LDSP-T, CS-T) respectively. Cas9 is fused to either the GFP or Nlux (with HA tag) reporters by a 3x glycine-serine linker (GSGSGS) and contains SV40 nuclear localization signals on the N’ and C’ termini (NLS). The gRNA scaffold and the 3’ self-cleaving HDV ribozyme is integrated into the vector. The 5’ hammerhead ribozyme (HH) specific for each guide sequence (GS, orange) is fused to the gRNA scaffold (scaffold) to form a sgRNA. Ribozymes are highlighted in yellow. Unique restriction sites are shown with an upwards line and the name in italics. (B) Confocal analysis of DAPI nuclear staining, Cas9-GFP signal, and merged brightfield, DAPI and GFP signal in N. oceanica cells. Scale bar of 2 μM. (C) Immunoblotting with an α-GFP antibody detected the Cas9-GFP produced in N. oceanica transformed with pNOC-CRISPR-GFP. A N. oceanica line producing a delta-5 fatty acid desaturase (~75 kDa) fused with CFP was used as a GFP positive control (+). (D) Immunoblotting with an α-HA antibody detected the appropriately sized Cas9-Nlux-HA in N. oceanica transformed with pNOC-CRISPR. Wild-type N. oceanica was included as a negative control (WT). For (C) and (D) numbers on the left of immunoblots indicate size markers (KDa).
    Hygromycin B, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    A one-vector CRISPR system for gene disruption in N. oceanica . (A) The pNOC-CRISPR vector series includes a Hygromycin B resistance cassette (HygC, green). A bidirectional promoter (Ribi) drives the transcription of the Cas9-reporter fusion and gRNA scaffold (scaffold, blue), along with the LDSP and CS terminators (LDSP-T, CS-T) respectively. Cas9 is fused to either the GFP or Nlux (with HA tag) reporters by a 3x glycine-serine linker (GSGSGS) and contains SV40 nuclear localization signals on the N’ and C’ termini (NLS). The gRNA scaffold and the 3’ self-cleaving HDV ribozyme is integrated into the vector. The 5’ hammerhead ribozyme (HH) specific for each guide sequence (GS, orange) is fused to the gRNA scaffold (scaffold) to form a sgRNA. Ribozymes are highlighted in yellow. Unique restriction sites are shown with an upwards line and the name in italics. (B) Confocal analysis of DAPI nuclear staining, Cas9-GFP signal, and merged brightfield, DAPI and GFP signal in N. oceanica cells. Scale bar of 2 μM. (C) Immunoblotting with an α-GFP antibody detected the Cas9-GFP produced in N. oceanica transformed with pNOC-CRISPR-GFP. A N. oceanica line producing a delta-5 fatty acid desaturase (~75 kDa) fused with CFP was used as a GFP positive control (+). (D) Immunoblotting with an α-HA antibody detected the appropriately sized Cas9-Nlux-HA in N. oceanica transformed with pNOC-CRISPR. Wild-type N. oceanica was included as a negative control (WT). For (C) and (D) numbers on the left of immunoblots indicate size markers (KDa).

    Journal: The Plant journal : for cell and molecular biology

    Article Title: Non-transgenic marker-free gene disruption by an episomal CRISPR system in the oleaginous microalga, Nannochloropsis oceanica CCMP1779

    doi: 10.1111/tpj.14314

    Figure Lengend Snippet: A one-vector CRISPR system for gene disruption in N. oceanica . (A) The pNOC-CRISPR vector series includes a Hygromycin B resistance cassette (HygC, green). A bidirectional promoter (Ribi) drives the transcription of the Cas9-reporter fusion and gRNA scaffold (scaffold, blue), along with the LDSP and CS terminators (LDSP-T, CS-T) respectively. Cas9 is fused to either the GFP or Nlux (with HA tag) reporters by a 3x glycine-serine linker (GSGSGS) and contains SV40 nuclear localization signals on the N’ and C’ termini (NLS). The gRNA scaffold and the 3’ self-cleaving HDV ribozyme is integrated into the vector. The 5’ hammerhead ribozyme (HH) specific for each guide sequence (GS, orange) is fused to the gRNA scaffold (scaffold) to form a sgRNA. Ribozymes are highlighted in yellow. Unique restriction sites are shown with an upwards line and the name in italics. (B) Confocal analysis of DAPI nuclear staining, Cas9-GFP signal, and merged brightfield, DAPI and GFP signal in N. oceanica cells. Scale bar of 2 μM. (C) Immunoblotting with an α-GFP antibody detected the Cas9-GFP produced in N. oceanica transformed with pNOC-CRISPR-GFP. A N. oceanica line producing a delta-5 fatty acid desaturase (~75 kDa) fused with CFP was used as a GFP positive control (+). (D) Immunoblotting with an α-HA antibody detected the appropriately sized Cas9-Nlux-HA in N. oceanica transformed with pNOC-CRISPR. Wild-type N. oceanica was included as a negative control (WT). For (C) and (D) numbers on the left of immunoblots indicate size markers (KDa).

    Article Snippet: Individual lines were transferred to 500 μl of F/2 with 100 μg/ml Hygromycin B (GoldBio) in a 96 deep well plate (Evergreen Biotech).

    Techniques: Plasmid Preparation, CRISPR, Sequencing, Staining, Produced, Transformation Assay, Positive Control, Negative Control, Western Blot

    Generation of marker-free non-transgenic mutants by episomal removal (curing). (A) Luminescence from equal number of cells of wild-type (WT), and NR-KO lines either containing the episome or cured of the episome. (B) PCR for detection of a positive control NR genomic locus and the Cas9 regions on the episome conducted on the same DNA extract obtained from WT and, NR-KO episomal and cured lines. (C) Plating of an equal number of cells of WT, NR-KO and NR-KO cured lines on NH 4 , NO 3 , and NH 4 with Hygromycin B on F/2 solid medium after 1 month of growth.

    Journal: The Plant journal : for cell and molecular biology

    Article Title: Non-transgenic marker-free gene disruption by an episomal CRISPR system in the oleaginous microalga, Nannochloropsis oceanica CCMP1779

    doi: 10.1111/tpj.14314

    Figure Lengend Snippet: Generation of marker-free non-transgenic mutants by episomal removal (curing). (A) Luminescence from equal number of cells of wild-type (WT), and NR-KO lines either containing the episome or cured of the episome. (B) PCR for detection of a positive control NR genomic locus and the Cas9 regions on the episome conducted on the same DNA extract obtained from WT and, NR-KO episomal and cured lines. (C) Plating of an equal number of cells of WT, NR-KO and NR-KO cured lines on NH 4 , NO 3 , and NH 4 with Hygromycin B on F/2 solid medium after 1 month of growth.

    Article Snippet: Individual lines were transferred to 500 μl of F/2 with 100 μg/ml Hygromycin B (GoldBio) in a 96 deep well plate (Evergreen Biotech).

    Techniques: Marker, Transgenic Assay, Polymerase Chain Reaction, Positive Control