rat p2x2  (Alomone Labs)


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    Structured Review

    Alomone Labs rat p2x2
    Expression of <t>P2X2</t> and P2X3 subunits in tissue sections of petrosal ganglia and carotid body as revealed by confocal immunofluorescence A – C , represent the same tissue section from the petrosal ganglion of a 15-day-old rat after immunostaining with P2X2 and P2X3 antibodies. Localization of P2X2 ( A ) and P2X3 ( B ) subunits is revealed by Cy3 and FITC fluorescence, respectively; dual exposure in C shows overlap of P2X2 and P2X3 staining. D – F , the same section from the carotid body of a 14-day-old rat after similar immunostaining with P2X2 and P2X3 antibodies as in A – C . Note complete overlap of P2X2 and P2X3 immunostaining in nerve terminals, which are apposed to lobules of chemoreceptor cells. Calibration bar represents 40 μm in A – C , and 50 μm in D – F .
    Rat P2x2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rat p2x2/product/Alomone Labs
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rat p2x2 - by Bioz Stars, 2022-05
    88/100 stars

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    1) Product Images from "Expression of P2X2 and P2X3 receptor subunits in rat carotid body afferent neurones: role in chemosensory signalling"

    Article Title: Expression of P2X2 and P2X3 receptor subunits in rat carotid body afferent neurones: role in chemosensory signalling

    Journal: The Journal of Physiology

    doi: 10.1111/j.1469-7793.2001.00667.x

    Expression of P2X2 and P2X3 subunits in tissue sections of petrosal ganglia and carotid body as revealed by confocal immunofluorescence A – C , represent the same tissue section from the petrosal ganglion of a 15-day-old rat after immunostaining with P2X2 and P2X3 antibodies. Localization of P2X2 ( A ) and P2X3 ( B ) subunits is revealed by Cy3 and FITC fluorescence, respectively; dual exposure in C shows overlap of P2X2 and P2X3 staining. D – F , the same section from the carotid body of a 14-day-old rat after similar immunostaining with P2X2 and P2X3 antibodies as in A – C . Note complete overlap of P2X2 and P2X3 immunostaining in nerve terminals, which are apposed to lobules of chemoreceptor cells. Calibration bar represents 40 μm in A – C , and 50 μm in D – F .
    Figure Legend Snippet: Expression of P2X2 and P2X3 subunits in tissue sections of petrosal ganglia and carotid body as revealed by confocal immunofluorescence A – C , represent the same tissue section from the petrosal ganglion of a 15-day-old rat after immunostaining with P2X2 and P2X3 antibodies. Localization of P2X2 ( A ) and P2X3 ( B ) subunits is revealed by Cy3 and FITC fluorescence, respectively; dual exposure in C shows overlap of P2X2 and P2X3 staining. D – F , the same section from the carotid body of a 14-day-old rat after similar immunostaining with P2X2 and P2X3 antibodies as in A – C . Note complete overlap of P2X2 and P2X3 immunostaining in nerve terminals, which are apposed to lobules of chemoreceptor cells. Calibration bar represents 40 μm in A – C , and 50 μm in D – F .

    Techniques Used: Expressing, Immunofluorescence, Immunostaining, Fluorescence, Staining

    Detection of mRNA for P2X2, P2X3 and β-actin in isolated petrosal neurones RT-PCR was carried out on groups of isolated petrosal neurones using gene-specific primers for P2X2 and P2X3 receptors, and β-actin. Expected product sizes are (bp): P2X2, 357; P2X3, 326; β actin, 327. The marker lane (M) shows bands at 100 bp increments with the 600 bp fragment at increased intensity. In negative control reactions without RT (-) no PCR products were observed. PCR products were visualized with 2 % agarose gel stained with ethidium bromide and viewed under UV illumination.
    Figure Legend Snippet: Detection of mRNA for P2X2, P2X3 and β-actin in isolated petrosal neurones RT-PCR was carried out on groups of isolated petrosal neurones using gene-specific primers for P2X2 and P2X3 receptors, and β-actin. Expected product sizes are (bp): P2X2, 357; P2X3, 326; β actin, 327. The marker lane (M) shows bands at 100 bp increments with the 600 bp fragment at increased intensity. In negative control reactions without RT (-) no PCR products were observed. PCR products were visualized with 2 % agarose gel stained with ethidium bromide and viewed under UV illumination.

    Techniques Used: Isolation, Reverse Transcription Polymerase Chain Reaction, Marker, Negative Control, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Staining

    Whole-cell current-clamp recording and single-cell RT-PCR products A , phase-contrast micrograph of co-cultured type 1 or glomus cell (GC) cluster and juxtaposed petrosal neurone (PN); this configuration was used to test for functional neurones in electrophysiological experiments. Scale bar represents 10 μm. Ba , whole-cell current-clamp recording from a single petrosal neurone juxtaposed to a type 1 cell cluster in co-culture (similar to A ). For the period indicated by the horizontal bar, hypoxia ( P O 2 ∼ 5 mmHg) was applied by the switching of the extracellular perfusate for one equilibrated with 100 % N 2 . Following recording, the cell contents were aspirated and subjected to RT-PCR analysis as described in Methods. Bb , 2 % agarose gel showing RT-PCR products from the cell in Ba , typical of results obtained in seven cells examined. Expected product sizes are (bp): P2X2, 357; P2X3, 326; β-actin, 327. The marker lane (M) shows bands at 100 bp increments with the 600 bp fragment at increased intensity. Lane C shows a negative control where extracellular perfusate, aspirated from above the cells in the recording chamber, was subjected to RT-PCR analysis as described using primers for P2X2, P2X3 and β-actin.
    Figure Legend Snippet: Whole-cell current-clamp recording and single-cell RT-PCR products A , phase-contrast micrograph of co-cultured type 1 or glomus cell (GC) cluster and juxtaposed petrosal neurone (PN); this configuration was used to test for functional neurones in electrophysiological experiments. Scale bar represents 10 μm. Ba , whole-cell current-clamp recording from a single petrosal neurone juxtaposed to a type 1 cell cluster in co-culture (similar to A ). For the period indicated by the horizontal bar, hypoxia ( P O 2 ∼ 5 mmHg) was applied by the switching of the extracellular perfusate for one equilibrated with 100 % N 2 . Following recording, the cell contents were aspirated and subjected to RT-PCR analysis as described in Methods. Bb , 2 % agarose gel showing RT-PCR products from the cell in Ba , typical of results obtained in seven cells examined. Expected product sizes are (bp): P2X2, 357; P2X3, 326; β-actin, 327. The marker lane (M) shows bands at 100 bp increments with the 600 bp fragment at increased intensity. Lane C shows a negative control where extracellular perfusate, aspirated from above the cells in the recording chamber, was subjected to RT-PCR analysis as described using primers for P2X2, P2X3 and β-actin.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Cell Culture, Functional Assay, Co-Culture Assay, Agarose Gel Electrophoresis, Marker, Negative Control

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    Alomone Labs rat p2x2
    Expression of <t>P2X2</t> and P2X3 subunits in tissue sections of petrosal ganglia and carotid body as revealed by confocal immunofluorescence A – C , represent the same tissue section from the petrosal ganglion of a 15-day-old rat after immunostaining with P2X2 and P2X3 antibodies. Localization of P2X2 ( A ) and P2X3 ( B ) subunits is revealed by Cy3 and FITC fluorescence, respectively; dual exposure in C shows overlap of P2X2 and P2X3 staining. D – F , the same section from the carotid body of a 14-day-old rat after similar immunostaining with P2X2 and P2X3 antibodies as in A – C . Note complete overlap of P2X2 and P2X3 immunostaining in nerve terminals, which are apposed to lobules of chemoreceptor cells. Calibration bar represents 40 μm in A – C , and 50 μm in D – F .
    Rat P2x2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rat p2x2/product/Alomone Labs
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rat p2x2 - by Bioz Stars, 2022-05
    88/100 stars
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    Expression of P2X2 and P2X3 subunits in tissue sections of petrosal ganglia and carotid body as revealed by confocal immunofluorescence A – C , represent the same tissue section from the petrosal ganglion of a 15-day-old rat after immunostaining with P2X2 and P2X3 antibodies. Localization of P2X2 ( A ) and P2X3 ( B ) subunits is revealed by Cy3 and FITC fluorescence, respectively; dual exposure in C shows overlap of P2X2 and P2X3 staining. D – F , the same section from the carotid body of a 14-day-old rat after similar immunostaining with P2X2 and P2X3 antibodies as in A – C . Note complete overlap of P2X2 and P2X3 immunostaining in nerve terminals, which are apposed to lobules of chemoreceptor cells. Calibration bar represents 40 μm in A – C , and 50 μm in D – F .

    Journal: The Journal of Physiology

    Article Title: Expression of P2X2 and P2X3 receptor subunits in rat carotid body afferent neurones: role in chemosensory signalling

    doi: 10.1111/j.1469-7793.2001.00667.x

    Figure Lengend Snippet: Expression of P2X2 and P2X3 subunits in tissue sections of petrosal ganglia and carotid body as revealed by confocal immunofluorescence A – C , represent the same tissue section from the petrosal ganglion of a 15-day-old rat after immunostaining with P2X2 and P2X3 antibodies. Localization of P2X2 ( A ) and P2X3 ( B ) subunits is revealed by Cy3 and FITC fluorescence, respectively; dual exposure in C shows overlap of P2X2 and P2X3 staining. D – F , the same section from the carotid body of a 14-day-old rat after similar immunostaining with P2X2 and P2X3 antibodies as in A – C . Note complete overlap of P2X2 and P2X3 immunostaining in nerve terminals, which are apposed to lobules of chemoreceptor cells. Calibration bar represents 40 μm in A – C , and 50 μm in D – F .

    Article Snippet: The primary antibodies were: (i) anti-P2X2 (1:800 dilution), a rabbit polyclonal antibody raised against a highly purified peptide corresponding to amino acid residues 457-472 of rat P2X2 (Alomone Laboratories, Jerusalem, Israel); and (ii) anti-P2X3 (1:500 dilution), a guinea-pig polyclonal antiserum raised against amino acid residues 383-397 of rat P2X3 (Neuromics, Minneapolis, MN, USA).

    Techniques: Expressing, Immunofluorescence, Immunostaining, Fluorescence, Staining

    Detection of mRNA for P2X2, P2X3 and β-actin in isolated petrosal neurones RT-PCR was carried out on groups of isolated petrosal neurones using gene-specific primers for P2X2 and P2X3 receptors, and β-actin. Expected product sizes are (bp): P2X2, 357; P2X3, 326; β actin, 327. The marker lane (M) shows bands at 100 bp increments with the 600 bp fragment at increased intensity. In negative control reactions without RT (-) no PCR products were observed. PCR products were visualized with 2 % agarose gel stained with ethidium bromide and viewed under UV illumination.

    Journal: The Journal of Physiology

    Article Title: Expression of P2X2 and P2X3 receptor subunits in rat carotid body afferent neurones: role in chemosensory signalling

    doi: 10.1111/j.1469-7793.2001.00667.x

    Figure Lengend Snippet: Detection of mRNA for P2X2, P2X3 and β-actin in isolated petrosal neurones RT-PCR was carried out on groups of isolated petrosal neurones using gene-specific primers for P2X2 and P2X3 receptors, and β-actin. Expected product sizes are (bp): P2X2, 357; P2X3, 326; β actin, 327. The marker lane (M) shows bands at 100 bp increments with the 600 bp fragment at increased intensity. In negative control reactions without RT (-) no PCR products were observed. PCR products were visualized with 2 % agarose gel stained with ethidium bromide and viewed under UV illumination.

    Article Snippet: The primary antibodies were: (i) anti-P2X2 (1:800 dilution), a rabbit polyclonal antibody raised against a highly purified peptide corresponding to amino acid residues 457-472 of rat P2X2 (Alomone Laboratories, Jerusalem, Israel); and (ii) anti-P2X3 (1:500 dilution), a guinea-pig polyclonal antiserum raised against amino acid residues 383-397 of rat P2X3 (Neuromics, Minneapolis, MN, USA).

    Techniques: Isolation, Reverse Transcription Polymerase Chain Reaction, Marker, Negative Control, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Staining

    Whole-cell current-clamp recording and single-cell RT-PCR products A , phase-contrast micrograph of co-cultured type 1 or glomus cell (GC) cluster and juxtaposed petrosal neurone (PN); this configuration was used to test for functional neurones in electrophysiological experiments. Scale bar represents 10 μm. Ba , whole-cell current-clamp recording from a single petrosal neurone juxtaposed to a type 1 cell cluster in co-culture (similar to A ). For the period indicated by the horizontal bar, hypoxia ( P O 2 ∼ 5 mmHg) was applied by the switching of the extracellular perfusate for one equilibrated with 100 % N 2 . Following recording, the cell contents were aspirated and subjected to RT-PCR analysis as described in Methods. Bb , 2 % agarose gel showing RT-PCR products from the cell in Ba , typical of results obtained in seven cells examined. Expected product sizes are (bp): P2X2, 357; P2X3, 326; β-actin, 327. The marker lane (M) shows bands at 100 bp increments with the 600 bp fragment at increased intensity. Lane C shows a negative control where extracellular perfusate, aspirated from above the cells in the recording chamber, was subjected to RT-PCR analysis as described using primers for P2X2, P2X3 and β-actin.

    Journal: The Journal of Physiology

    Article Title: Expression of P2X2 and P2X3 receptor subunits in rat carotid body afferent neurones: role in chemosensory signalling

    doi: 10.1111/j.1469-7793.2001.00667.x

    Figure Lengend Snippet: Whole-cell current-clamp recording and single-cell RT-PCR products A , phase-contrast micrograph of co-cultured type 1 or glomus cell (GC) cluster and juxtaposed petrosal neurone (PN); this configuration was used to test for functional neurones in electrophysiological experiments. Scale bar represents 10 μm. Ba , whole-cell current-clamp recording from a single petrosal neurone juxtaposed to a type 1 cell cluster in co-culture (similar to A ). For the period indicated by the horizontal bar, hypoxia ( P O 2 ∼ 5 mmHg) was applied by the switching of the extracellular perfusate for one equilibrated with 100 % N 2 . Following recording, the cell contents were aspirated and subjected to RT-PCR analysis as described in Methods. Bb , 2 % agarose gel showing RT-PCR products from the cell in Ba , typical of results obtained in seven cells examined. Expected product sizes are (bp): P2X2, 357; P2X3, 326; β-actin, 327. The marker lane (M) shows bands at 100 bp increments with the 600 bp fragment at increased intensity. Lane C shows a negative control where extracellular perfusate, aspirated from above the cells in the recording chamber, was subjected to RT-PCR analysis as described using primers for P2X2, P2X3 and β-actin.

    Article Snippet: The primary antibodies were: (i) anti-P2X2 (1:800 dilution), a rabbit polyclonal antibody raised against a highly purified peptide corresponding to amino acid residues 457-472 of rat P2X2 (Alomone Laboratories, Jerusalem, Israel); and (ii) anti-P2X3 (1:500 dilution), a guinea-pig polyclonal antiserum raised against amino acid residues 383-397 of rat P2X3 (Neuromics, Minneapolis, MN, USA).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Cell Culture, Functional Assay, Co-Culture Assay, Agarose Gel Electrophoresis, Marker, Negative Control