Probdnf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
Average 92 stars, based on 6 article reviews
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1) Product Images from "Dexmedetomidine Attenuates Neurotoxicity in Developing Rats Induced by Sevoflurane through Upregulating BDNF-TrkB-CREB and Downregulating ProBDNF-P75NRT-RhoA Signaling Pathway"
Article Title: Dexmedetomidine Attenuates Neurotoxicity in Developing Rats Induced by Sevoflurane through Upregulating BDNF-TrkB-CREB and Downregulating ProBDNF-P75NRT-RhoA Signaling Pathway
Journal: Mediators of Inflammation
Figure Legend Snippet: Dexmedetomidine could decrease the level of proBDNF and restore the ratio of proBDNF/mBDNF and alleviates activation of the proBDNF-P75NRT-RHOA pathway after sevoflurane. (a) Western blot band. (b) ProBDNF/mBDNF. (c–e) Bar graph of Western blot. (f) Immunofluorescence of proBDNF (scale bar = 50 μ m). ∗ Compare with the control group, P
Techniques Used: Activation Assay, Western Blot, Immunofluorescence
2) Product Images from "The decline in synaptic GluN2B and rise in inhibitory neurotransmission determine the end of a critical period"
Article Title: The decline in synaptic GluN2B and rise in inhibitory neurotransmission determine the end of a critical period
Journal: Scientific Reports
Figure Legend Snippet: CS synapses in the ventral spinal cord were eliminated upon increasing synaptic 2B. ( A ) Reduction of CS synaptic activity in the ventral side after the critical period in 2a −/− mice. Spatial distribution of CS synapses determined using optical CS-EPSPs in WT, proBDNF-treated or 2a −/− mice at 11–13 or 14–16 DIV. Scale bar = 250 μm. ( B ) Ventrodorsal ratios of optical CS-EPSPs in the indicated culture pairs from 11–13 DIV (0.40 ± 0.03 in WT mice, n = 12, Ns = 12, Nm = 6; 0.42 ± 0.03 in 2a −/− mice, n = 6, Ns = 6, Nm = 6) or from 14–16 DIV (0.46 ± 0.02 in WT mice, n = 17, Ns = 17, Nm = 8; 0.39 ± 0.02 in proBDNF-treated mice, n = 4, Ns = 4, Nm = 4; 0.31 ± 0.03 in 2a −/− mice, n = 7, Ns = 7, Nm = 6).
Techniques Used: Activity Assay, Mouse Assay
Figure Legend Snippet: Decline in 2B-containing NMDAR across the end of the critical period. ( A ) Developmental alterations of the total NMDA component of CS-EPSCs in the spinal cord. Averaged CS-EPSC traces recorded after application of NBQX to WT ( upper ) or proBDNF-treated ( middle ) and 2a −/− ( lower ) mice before ( black ) or after ( red ) Ro25-6981 treatment on 12 DIV. Calibration: 50 pA, 100ms. ( B ) Averaged 2B-CS-EPSCs in WT (55.0 ± 14.6 pA, 6–8 DIV, n = 21, Ns = 16, Nm = 32; 18.0 ± 1.99 pA, 12–15 DIV, n = 18, Ns = 10, Nm = 20), proBDNF-treated (44.1 ± 6.45 pA, 12–15 DIV, n = 13, Ns = 8, Nm = 16) and 2a −/− mice (54.2 ± 10.8 pA, 6–8 DIV, n = 7, Ns = 7, Nm = 14; 74.4 ± 30.1 pA, 12–15 DIV, n = 7, Ns = 6, Nm = 12). ( C ) Developmental alterations of synaptic 2B in WT or 2a −/− mice. Immunoblot analysis of synaptic expression of 2B (2B in SPMs), PSD-95 (in SPMs) and MAGUKs (in SPMs) in WT and 2a −/− mice. Full-length blots are presented in Supplementary Fig. 7S . (D) Ratio of 2B to PSD-95 or MAGUKs in SPMs: To quantitate synaptic 2B, the intensity of 2B in SPMs was normalized to that of PSD-95 or MAGUK in SPMs in WT or 2a −/− spinal cords (Ratio of 2B to PSD-95: 1.00, 6 DIV in WT mice; 0.44, 13 DIV in WT mice; 0.62, 6 DIV in 2a −/− mice; 0.66, 13 DIV in 2a −/− mice. Ratio of 2B to MAGUKs: 1.00, 6 DIV in WT mice; 0.48, 13 DIV in WT mice; 0.75, 6 DIV in 2a −/− mice; 0.66, 13 DIV in 2a −/− mice. Each sample represents an SPM extract prepared from 20 co-cultures from 4 mice). Data are represented as the mean ± standard error of the mean. Asterisks indicate statistical significance (Student t test). * p
Techniques Used: Mouse Assay, Expressing
Figure Legend Snippet: CS axons in the ventral spinal cord regressed upon increasing synaptic 2B. ( A ) Images of CS axons labeled with ChR2-EYFP in WT ( left ), proBDNF-treated and 2a −/− spinal explants ( right ). The first image was taken at 12 DIV and the second at 15 or 16 DIV. Enumeration of CS axons on the ventral side that crossed the 70% line ( red bold line ) from the dorsal to the ventral edge of spinal gray matter. Scale bar = 250 µm. ( B ) Ratios of CS axons on the ventral side at 15 or 16 DIV to that at 12 DIV (15 DIV/12 DIV: 1.13 ± 0.06 in WT mice, n = 9, Ns = 9, Nm = 4; 16 DIV/12 DIV: 0.60 ± 0.05 in proBDNF-treated mice, n = 11, Ns = 11, Nm = 6; 0.81 ± 0.05 in 2a −/− mice, n = 11, Ns = 11, Nm = 8) ( right ).
Techniques Used: Labeling, Mouse Assay
3) Product Images from "BDNF–TrkB signaling in the nucleus accumbens shell of mice has key role in methamphetamine withdrawal symptoms"
Article Title: BDNF–TrkB signaling in the nucleus accumbens shell of mice has key role in methamphetamine withdrawal symptoms
Journal: Translational Psychiatry
Figure Legend Snippet: Depression-like behavior and levels of proBDNF and BDNF in the brain regions after withdrawal from repeated METH exposure. ( a ) Schedule of treatment and behavioral tests. Saline (10 ml kg −1 per day for 5 days) or METH (3 mg kg −1 per day for 5 days) was injected into mice. Behavioral tests were performed at days 7, 11 and 18 (SPT), and days 8, 12 and 19 (LMT, TST, FST). ( b ) SPT: sucrose preference of METH-treated mice was significantly lower than that of control (saline-treated) mice. LMT: there were no differences between control and METH-treated mice. TST and FST: the immobility time of METH-treated mice was significantly higher than that of control mice. * P
Techniques Used: Injection, Mouse Assay, Single-particle Tracking
4) Product Images from "Human Immunodeficiency Virus-1 Alters Brain-derived Neurotrophic Factor Processing in neurons"
Article Title: Human Immunodeficiency Virus-1 Alters Brain-derived Neurotrophic Factor Processing in neurons
Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience
Figure Legend Snippet: Neuronal localization of proBDNF in human brains
Figure Legend Snippet: pBDNF and proBDNF levels in HIV subjects
Figure Legend Snippet: gp120 alters the release of mBDNF/proBDNF in cortical neurons
Figure Legend Snippet: gp120 increases proBDNF