bdnf  (Alomone Labs)


Bioz Verified Symbol Alomone Labs is a verified supplier
Bioz Manufacturer Symbol Alomone Labs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Alomone Labs bdnf
    Axon directionality on 1400 nm topographical pitch surface or 0 nm pitch flat surface is not altered by diffuse soluble factors. DRG were plated onto either 0 nm pitch control surfaces or 1400 nm pitch surfaces with a basal media enriched with either NGF, GDNF, <t>BDNF</t> or NT-3. Six days after plating, DRG were fixed, stained and imaged using confocal microscopy. The directionality of axons 1500 µm from the DRG body was quantified. DRG cultured on the 1400 nm pitch topography had 85–100% of their axons parallel to the underlying surface topography, independent of the soluble growth factor that was added to the media. The DRG cultured on the 0 nm pitch control substrates had individual axons that ranged from 0–100% parallel to an arbitrary, consistent axis. Each 1400 nm pitch category was significantly different (** = p
    Bdnf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bdnf/product/Alomone Labs
    Average 94 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    bdnf - by Bioz Stars, 2022-12
    94/100 stars

    Images

    1) Product Images from "Submicron Topographically Patterned 3D Substrates Enhance Directional Axon Outgrowth of Dorsal Root Ganglia Cultured Ex Vivo"

    Article Title: Submicron Topographically Patterned 3D Substrates Enhance Directional Axon Outgrowth of Dorsal Root Ganglia Cultured Ex Vivo

    Journal: Biomolecules

    doi: 10.3390/biom12081059

    Axon directionality on 1400 nm topographical pitch surface or 0 nm pitch flat surface is not altered by diffuse soluble factors. DRG were plated onto either 0 nm pitch control surfaces or 1400 nm pitch surfaces with a basal media enriched with either NGF, GDNF, BDNF or NT-3. Six days after plating, DRG were fixed, stained and imaged using confocal microscopy. The directionality of axons 1500 µm from the DRG body was quantified. DRG cultured on the 1400 nm pitch topography had 85–100% of their axons parallel to the underlying surface topography, independent of the soluble growth factor that was added to the media. The DRG cultured on the 0 nm pitch control substrates had individual axons that ranged from 0–100% parallel to an arbitrary, consistent axis. Each 1400 nm pitch category was significantly different (** = p
    Figure Legend Snippet: Axon directionality on 1400 nm topographical pitch surface or 0 nm pitch flat surface is not altered by diffuse soluble factors. DRG were plated onto either 0 nm pitch control surfaces or 1400 nm pitch surfaces with a basal media enriched with either NGF, GDNF, BDNF or NT-3. Six days after plating, DRG were fixed, stained and imaged using confocal microscopy. The directionality of axons 1500 µm from the DRG body was quantified. DRG cultured on the 1400 nm pitch topography had 85–100% of their axons parallel to the underlying surface topography, independent of the soluble growth factor that was added to the media. The DRG cultured on the 0 nm pitch control substrates had individual axons that ranged from 0–100% parallel to an arbitrary, consistent axis. Each 1400 nm pitch category was significantly different (** = p

    Techniques Used: Staining, Confocal Microscopy, Cell Culture

    2) Product Images from "Effects of Exercise Training during Advanced Maternal Age on the Cognitive Function of Offspring"

    Article Title: Effects of Exercise Training during Advanced Maternal Age on the Cognitive Function of Offspring

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms23105517

    Effects of exercise on BDNF and PSD95 in the hippocampus of the offspring from mothers with advanced maternal age (AMA). CON: offspring from the control group; CON + EX: offspring from the control and exercised group; AMA: offspring from the advanced maternal age group; AMA + EX: offspring from the advanced maternal age and exercised group. Data are expressed as means ± standard error of the mean (SEM). * p
    Figure Legend Snippet: Effects of exercise on BDNF and PSD95 in the hippocampus of the offspring from mothers with advanced maternal age (AMA). CON: offspring from the control group; CON + EX: offspring from the control and exercised group; AMA: offspring from the advanced maternal age group; AMA + EX: offspring from the advanced maternal age and exercised group. Data are expressed as means ± standard error of the mean (SEM). * p

    Techniques Used:

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Alomone Labs brain derived neurotrophic factor
    (A) The sequence of TRKB transmembrane (TM) domain from rat (UniProt: Q63604, residues 430-453) was mutated at Y433 residue (Y433F) or the entire motif was substituted by the rat sequence of TRKA TM (P35739, residues 419-442). (B) The interaction between <t>biotinylated</t> <t>BDNF</t> (bBDNF) and TRKB is not affected by the TRKB.Y433F mutation or in TRKB/TRKA.TM constructs. (C) TRKB.Y433F does not prevent the BDNF-induced dimerization of TRKB. The constructs used allow the formation of TRKB.wt homodimer or TRKB.wt/Y433F heterodimer. (D , E) Analysis of TRKB phosphorylation shows that, cortical cultures from TRKB.Y433F heterozygous mouse embryos respond to BDNF similarly to the the TRKB.wt littermates, regardless of the tyrosine residue tested ( D : Y515, E : Y816).
    Brain Derived Neurotrophic Factor, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/brain derived neurotrophic factor/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    brain derived neurotrophic factor - by Bioz Stars, 2022-12
    94/100 stars
      Buy from Supplier

    Image Search Results


    (A) The sequence of TRKB transmembrane (TM) domain from rat (UniProt: Q63604, residues 430-453) was mutated at Y433 residue (Y433F) or the entire motif was substituted by the rat sequence of TRKA TM (P35739, residues 419-442). (B) The interaction between biotinylated BDNF (bBDNF) and TRKB is not affected by the TRKB.Y433F mutation or in TRKB/TRKA.TM constructs. (C) TRKB.Y433F does not prevent the BDNF-induced dimerization of TRKB. The constructs used allow the formation of TRKB.wt homodimer or TRKB.wt/Y433F heterodimer. (D , E) Analysis of TRKB phosphorylation shows that, cortical cultures from TRKB.Y433F heterozygous mouse embryos respond to BDNF similarly to the the TRKB.wt littermates, regardless of the tyrosine residue tested ( D : Y515, E : Y816).

    Journal: bioRxiv

    Article Title: Mutation in the TRKB cholesterol recognition site that blocks antidepressant binding does not influence the basal or BDNF-stimulated activation of TRKB

    doi: 10.1101/2022.08.26.505413

    Figure Lengend Snippet: (A) The sequence of TRKB transmembrane (TM) domain from rat (UniProt: Q63604, residues 430-453) was mutated at Y433 residue (Y433F) or the entire motif was substituted by the rat sequence of TRKA TM (P35739, residues 419-442). (B) The interaction between biotinylated BDNF (bBDNF) and TRKB is not affected by the TRKB.Y433F mutation or in TRKB/TRKA.TM constructs. (C) TRKB.Y433F does not prevent the BDNF-induced dimerization of TRKB. The constructs used allow the formation of TRKB.wt homodimer or TRKB.wt/Y433F heterodimer. (D , E) Analysis of TRKB phosphorylation shows that, cortical cultures from TRKB.Y433F heterozygous mouse embryos respond to BDNF similarly to the the TRKB.wt littermates, regardless of the tyrosine residue tested ( D : Y515, E : Y816).

    Article Snippet: The plates were then washed 3x with PBS buffer, and various concentrations of biotinylated BDNF (Alomone Labs, cat#B-250, 0.1 – 100 pM) was added for 1h at RT.

    Techniques: Sequencing, Mutagenesis, Construct