rabbit anti kv1 1 (Alomone Labs)


Structured Review

Rabbit Anti Kv1 1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti kv1 1/product/Alomone Labs
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Kv1.1 channelopathy abolishes presynaptic spike width modulation by subthreshold somatic depolarization"
Article Title: Kv1.1 channelopathy abolishes presynaptic spike width modulation by subthreshold somatic depolarization
Journal: Proceedings of the National Academy of Sciences of the United States of America
doi: 10.1073/pnas.1608763114

Figure Legend Snippet: Kv1.1 channels determine spike width. ( A ) Example recordings from one neuron before and after 20 nM DTx-K application. This neurotoxin had no effect on somatic spikes but led to broadening of presynaptic action potentials. ( B ) Presynaptic spike widths
Techniques Used:

Figure Legend Snippet: Blockade or deletion of Kv1.1 does not prevent analog modulation of presynaptic spike width. ( A ) Trace analysis from one wild-type cell showing bidirectional changes in presynaptic spike width by subthreshold somatic current injections before evoking
Techniques Used:
2) Product Images from "Potassium channels Kv1.1, Kv1.2 and Kv1.6 influence excitability of rat visceral sensory neurons"
Article Title: Potassium channels Kv1.1, Kv1.2 and Kv1.6 influence excitability of rat visceral sensory neurons
Journal: The Journal of Physiology
doi: 10.1113/jphysiol.2001.018333

Figure Legend Snippet: Kv1.1, Kv1.2 and Kv1.6 are detected in Western blots of nodose ganglia and brain protein Western blots of channel expression in nodose ganglia ( A , B and C ), brain lysates ( A and B ) and brain crude membrane fraction ( C ) probed with monoclonal anti-Kv1.1 ( A ), monoclonal anti-Kv1.2 ( B ) and polyclonal anti-Kv1.6 ( C ) (50 μg protein per lane). Immunoreactive bands were visualized with ECL-Plus (Amersham Pharmacia Biotech). Molecular weight markers (kDa) are indicated on the left.
Techniques Used: Western Blot, Expressing, Molecular Weight
3) Product Images from "The mono-ADP-ribosyltransferase ARTD10 regulates the voltage-gated K+ channel Kv1.1 through protein kinase C delta"
Article Title: The mono-ADP-ribosyltransferase ARTD10 regulates the voltage-gated K+ channel Kv1.1 through protein kinase C delta
Journal: BMC Biology
doi: 10.1186/s12915-020-00878-1

Figure Legend Snippet: ARTD10 inhibition reduces the proportion of the inactivating Kv1.1 current and enhances spontaneous excitation in hippocampal neurons. a Left, whole cell currents of mouse hippocampal neurons in the presence of tetrodotoxin with and without an inhibitor of ARTD10 (OUL35). Right, bar graphs representing I steady-state / I peak ( I s / I p ) and peak current amplitudes. b Resting membrane potential (RMP) and spontaneous spikes/s from control and OUL35 treated hippocampal neurons. * p
Techniques Used: Inhibition

Figure Legend Snippet: Scheme illustrating the interplay of PKA, PKCδ, and ARTD10 in the regulation of Kv1.1. The regulation of a phosphatase by PKCδ is hypothetical. Current traces above the schemes of the Kv1.1 channels illustrate the typical inactivation pattern of phosphorylated vs. un-phosphorylated Kv1.1α. I/F, IBMX/forskolin; PMA, phorbol-myristate-acetate
Techniques Used:

Figure Legend Snippet: ARTD10 inhibition enhances excitability of hippocampal neurons via Kv1.1. a Left, representative current clamp recordings of APs elicited by step current pulses in control neurons and neurons treated with OUL35. Right, bar graphs represent the rheobase and the latency to the first spike. For cells with a RMP more positive than − 60 mV, the membrane potential was adjusted to ~ − 60 mV. b The number of spikes elicited by step current pulses were counted and for stimuli from 10 to 30 pA they were fitted with a linear function. Right, bar graphs summarize the AP amplitude from neurons with and without OUL35 treatment. c Left, bar graphs representing the rheobase. Right, summary of spikes/s with and without the Kv1 inhibitor α-dendrotoxin (DTX) and from neurons with and without OUL35 treatment. * p
Techniques Used: Inhibition

Figure Legend Snippet: Inactivation of Kv1.1 is regulated by Kvβ and phosphorylation at S446. a Representative recordings, I steady-state / I peak ( I s / I p ), and peak current amplitudes of Kv1.1 with or without co-expression of Kvβ1.1 in HeLa cells. b Representative recordings and I s / I p before and after the application of either IBMX/forskolin (I/F) or of the phorbol ester PMA. c Representative recordings, I s / I p , and peak current amplitudes of the phosphorylation-deficient mutant Kv1.1 S446A co-expressed with Kvβ1.1. d Representative recordings, I s / I p , and peak current amplitudes of Kv1.1 after 1.5 h pre-incubation with a phosphatase inhibitor cocktail. * p
Techniques Used: Expressing, Mutagenesis, Incubation

Figure Legend Snippet: ARTD10 leads to phosphorylation of Kv1.1 at S446. a , b Left, Representative current traces of wild type ( a ) or the phosphorylation-deficient mutant ( b ) of Kv1.1 overexpressed in three different HeLa cell lines. Right, bar graphs representing I steady-state / I peak ( I s / I p ) and peak current amplitudes. * p
Techniques Used: Mutagenesis

Figure Legend Snippet: Kv1.1 is regulated by PKCδ in HeLa cells. a , b Wild type Kv1.1 or mutant Kv1.1-S446A were co-expressed with Kvβ1 and either the catalytic domain (CAT) or a dominant negative mutant (DN) of PKCδ. Bar graphs represent I steady-state / I peak ( I s / I p ) and peak current amplitudes
Techniques Used: Mutagenesis, Dominant Negative Mutation