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biotinylated sambucus nigra lectin sna  (Vector Laboratories)


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    Vector Laboratories biotinylated sambucus nigra lectin sna
    Biotinylated Sambucus Nigra Lectin Sna, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 649 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated sambucus nigra lectin sna/product/Vector Laboratories
    Average 96 stars, based on 649 article reviews
    biotinylated sambucus nigra lectin sna - by Bioz Stars, 2026-02
    96/100 stars

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    TSC1 deficiency remodels sialic acid metabolic homeostasis via the mTORC1-S6K1 signaling pathway and promotes tumor immune escape. (A) qPCR analysis of ST6GALNAC1, Neu4, and NPL mRNA expression. (B) Representative Western blot images showing the expression levels of ST6GALNAC1, Neu4, and PD-L1 in indicated groups. (C) Analysis of PD-L1 sialylation levels by <t>lectin</t> blot assay. (D) Assessment of CD8+ T cell cytotoxic function. Data are presented as mean ± SEM (n=3). *** p < 0.001, ** p < 0.01, * p < 0.05 vs control group.
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    TSC1 deficiency remodels sialic acid metabolic homeostasis via the mTORC1-S6K1 signaling pathway and promotes tumor immune escape. (A) qPCR analysis of ST6GALNAC1, Neu4, and NPL mRNA expression. (B) Representative Western blot images showing the expression levels of ST6GALNAC1, Neu4, and PD-L1 in indicated groups. (C) Analysis of PD-L1 sialylation levels by lectin blot assay. (D) Assessment of CD8+ T cell cytotoxic function. Data are presented as mean ± SEM (n=3). *** p < 0.001, ** p < 0.01, * p < 0.05 vs control group.

    Journal: Frontiers in Immunology

    Article Title: TSC1 deficiency drives immune evasion in colorectal cancer via mTORC1-mediated dysregulation of PD-L1 sialylation

    doi: 10.3389/fimmu.2025.1692210

    Figure Lengend Snippet: TSC1 deficiency remodels sialic acid metabolic homeostasis via the mTORC1-S6K1 signaling pathway and promotes tumor immune escape. (A) qPCR analysis of ST6GALNAC1, Neu4, and NPL mRNA expression. (B) Representative Western blot images showing the expression levels of ST6GALNAC1, Neu4, and PD-L1 in indicated groups. (C) Analysis of PD-L1 sialylation levels by lectin blot assay. (D) Assessment of CD8+ T cell cytotoxic function. Data are presented as mean ± SEM (n=3). *** p < 0.001, ** p < 0.01, * p < 0.05 vs control group.

    Article Snippet: Membranes were blocked with 5% non-fat dry milk in TBST for 1 h at room temperature before incubation with biotinylated Sambucus nigra lectin (SNA, Vector Laboratories; Cat# B-1305; 1:1000 dilution in blocking buffer) at 4°C overnight to specifically detect α2,6-linked sialic acid modifications.

    Techniques: Expressing, Western Blot, Control