Journal: bioRxiv
Article Title: Substrate recognition and cleavage by mucin degrading O -glycopeptidases from the gut microbe Bacteroides caccae
doi: 10.1101/2025.10.14.682407
Figure Lengend Snippet: Mucinase assay showing the activity of twelve B. caccae peptidase_M60 proteins on bovine maxillary mucin (BSM). A reduction in signal indicates loss of immobilized biotinylated BSM and therefore mucinase assay. Pairwise comparisons of the no-EDTA samples to the BSA control all gave P-values <0.0001, except for BM60B, which is indicated in the figure. Pairwise comparisons of the EDTA samples to the BSA EDTA control sample all gave P-values > 0.05. IMPa was included as a positive control. P-value symbols for unpaired two-tailed T-tests are as follows: P<0.05 (*), P<0.01 (**), P<0.001 (***), P<0.0001 (****).
Article Snippet: Biotinylated VVA and PNA were purchased from Vector Laboratories.
Techniques: Activity Assay, Control, Positive Control, Two Tailed Test