rabbit polyclonal anti p2rx7 extracellular antibody (Alomone Labs)

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Name:

Anti P2X7 Receptor extracellular Antibody

Description:

Catalog Number:

APR-008

Price:

397.0

Category:

Primary Antibody

Applications:

Immunocytochemistry, Immunofluorescence, Indirect Flow Cytometry, Immunohistochemistry, Live Cell Imaging, Western Blot

Purity:

Affinity purified on immobilized antigen.

Immunogen:

Synthetic peptide

Size:

25 mcl

Antibody Type:

Polyclonal Primary Antibodies

Format:

Lyophilized Powder

Host:

Rabbit

Isotype:

Rabbit IgG

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Structured Review

Alomone Labs rabbit polyclonal anti p2rx7 extracellular antibody

Anti P2X7 Receptor extracellular Antibody APR 008 is a highly specific antibody directed against an extracellular epitope of the mouse protein The antibody can be used in western blot indirect flow cytometry immunohistochemistry live cell imaging and immunocytochemistry applications It has been designed to recognize P2X7 purinergic receptor from mouse rat and human samples
https://www.bioz.com/result/rabbit polyclonal anti p2rx7 extracellular antibody/product/Alomone Labs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99

rabbit polyclonal anti p2rx7 extracellular antibody - by Bioz Stars, 2021-09

94/100 stars

Images

1) Product Images from "Nucleoside reverse transcriptase inhibitors and Kamuvudines inhibit amyloid-β induced retinal pigmented epithelium degeneration"

Article Title: Nucleoside reverse transcriptase inhibitors and Kamuvudines inhibit amyloid-β induced retinal pigmented epithelium degeneration

Journal: Signal Transduction and Targeted Therapy

doi: 10.1038/s41392-021-00537-z

P2RX7 expression is required for AβOs-induced RPE degeneration. Eyes were treated with a single subretinal injection of 1 μM AβOs. Tissue was collected 7 days after injection. a P2rx7 −/− mice are protected from AβOs-induced RPE degeneration, n = 8. b Lower magnification (left panel) and higher magnification (right panel) of RPE flat mounts of P2rx7 hP2RX7Flox and P2rx7 hP2RX7Flox / Best1-Cre+ mice stained with phalloidin (white) and P2RX7 (yellow) demonstrating reduction of P2RX7 signal in the RPE of P2rx7 hP2RX7Flox / Best1-Cre+ mice compared to P2rx7 hP2RX7Flox mice. Black arrowhead points to the optic nerve of P2rx7 hP2RX7Flox / Best1-Cre+ mice, where expression of P2RX persists in non-RPE tissue. c AβOs induced degeneration in P2rx7 hP2RX7Flox ( n = 6) but not in P2rx7 hP2RX7Flox / Best1-Cre+ mice ( n = 8) ( d ). Representative images are shown. Fundus photographs, top row; Flat mounts stained for zonula occludens-1 (ZO-1; red), bottom row. Degeneration outlined by white arrowheads. Binary (Healthy %) and morphometric (PM, polymegethism (mean (SEM)) quantification of RPE degeneration is shown (Fisher’s exact test for binary; two-tailed t -test for morphometry; * P

Figure Legend Snippet: P2RX7 expression is required for AβOs-induced RPE degeneration. Eyes were treated with a single subretinal injection of 1 μM AβOs. Tissue was collected 7 days after injection. a P2rx7 −/− mice are protected from AβOs-induced RPE degeneration, n = 8. b Lower magnification (left panel) and higher magnification (right panel) of RPE flat mounts of P2rx7 hP2RX7Flox and P2rx7 hP2RX7Flox / Best1-Cre+ mice stained with phalloidin (white) and P2RX7 (yellow) demonstrating reduction of P2RX7 signal in the RPE of P2rx7 hP2RX7Flox / Best1-Cre+ mice compared to P2rx7 hP2RX7Flox mice. Black arrowhead points to the optic nerve of P2rx7 hP2RX7Flox / Best1-Cre+ mice, where expression of P2RX persists in non-RPE tissue. c AβOs induced degeneration in P2rx7 hP2RX7Flox ( n = 6) but not in P2rx7 hP2RX7Flox / Best1-Cre+ mice ( n = 8) ( d ). Representative images are shown. Fundus photographs, top row; Flat mounts stained for zonula occludens-1 (ZO-1; red), bottom row. Degeneration outlined by white arrowheads. Binary (Healthy %) and morphometric (PM, polymegethism (mean (SEM)) quantification of RPE degeneration is shown (Fisher’s exact test for binary; two-tailed t -test for morphometry; * P

Techniques Used: Expressing, Injection, Mouse Assay, Staining, Two Tailed Test

Staining:

Article Title: Nucleoside reverse transcriptase inhibitors and Kamuvudines inhibit amyloid-β induced retinal pigmented epithelium degeneration
Article Snippet: .. The RPE flat mounts were stained with Dylight phalloidin 650 (1:10, Cell Signaling) and a rabbit polyclonal anti-P2RX7 (extracellular) antibody (1:100, Alomone Labs), followed by a goat anti-rabbit Alexa-555 antibody (1:200, Invitrogen). ..

Incubation:

Article Title: P2X7 Receptor Upregulation in Huntington’s Disease Brains
Article Snippet: .. Membranes were incubated overnight at 4°C with different anti-P2X7R antibodies directed to C-terminus (rabbit, 1:1,000, Alomone Lab, APR-004), N-terminus (goat, 1:500, NovusBio, NBP1-37775), or extracellular domain (rabbit, 1:200, Alomone Lab, APR-008) in TBS-T supplemented with 5% non-fat dry milk, washed with TBS-T, and next incubated with HRP-conjugated anti-rabbit IgG (1:2,000, DAKO, P0448) or anti-goat IgG (1:5,000, Bethyl, A50-101P) and developed using the ECL detection kit (PerkinElmer, NEL105001EA). ..

Article Title: Differential sensitivity of acute myeloid leukemia cells to daunorubicin depends on P2X7A versus P2X7B receptor expression
Article Snippet: .. Membranes were incubated overnight at 4 °C with primary antibodies as follows: anti-P2X7 (P8232, Sigma-Aldrich) antibody, recognizing the C terminal domain of the protein was diluted 1:300, anti-P2X7 (APR008, Alomone Labs, Jerusalem, Israel) antibody, recognizing the extracellular loop of the protein was diluted 1:300, anti-c-myc antibody (A190-105A, Bethyl Laboratories, INC) was diluted 1:2500, and anti-SKP-1 antibody (MA5-15928, Thermo Scientific) was diluted 1:1000. ..

Immunohistochemistry:

Article Title: P2X7 Receptor Promotes Mouse Mammary Cancer Cell Invasiveness and Tumour Progression, and Is a Target for Anticancer Treatment
Article Snippet: .. Immunohistochemistry was performed using a primary antibody anti-P2X7 (APR-008 Alomone Labs Ltd.) and streptavidin-biotin-peroxidase method with diaminobenzidine as the chromogen (Kit LSAB, Dakocytomation, Dako France SAS, Les Ullis, France). ..

Proximity Ligation Assay:

Article Title: Pannexin1: Role as a Sensor to Injury Is Attenuated in Pretype 2 Corneal Diabetic Epithelium
Article Snippet: .. For proximity ligation assay, anti-pannexin1 monoclonal mouse antibody directed against pannexin1 (Cat. #SC-515941) was purchased from Santa Cruz Biotechnology (Dallas, TX), and anti-P2X7 polyclonal rabbit antibody targeting the extracellular domain of P2X7 receptors (Cat. #APR-008) was purchased from Alomone Labs. ..