anti vpac1 vipr1 extracellular antibody  (Alomone Labs)


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    Alomone Labs anti vpac1 vipr1 extracellular antibody
    Retinoic acid induces <t>Vipr1</t> gene expression. (A) SMG organoids were treated with or without RA (300 nM) for the indicated time. After harvesting, the organoids were subjected to qRT-PCR to evaluate gene expression associated with VIP signaling ( n = 4). (B) SMG organoids were treated with varying doses of RA for 7 days and subjected to qRT-PCR for the evaluation of gene expression of Vipr1 and Rarb , a positive control, for RA-mediated signaling ( n = 4). (C) Expression of VIPR1 (green) in mouse SMG tissues (left) was assessed by co-staining with KRT5 (magenta, left), KRT7 (cyan, left), ACTA2 (magenta, right), and MIST1 (cyan, right). Nuclei were counterstained with DAPI (white) and each single channel image was placed below. Scale bars indicate 20 μm. (D) The mouse SMG organoids cultured with (bottom) or without RA (top) were subjected to immunofluorescence for the evaluation of VIPR1 (green), KRT5 (magenta), and KRT7 (cyan) expressions. Nuclei were counterstained with DAPI (white), and each single channel image was placed below. Scale bars indicate 50 μm. (E) The expression of several salivary gland markers in organoids treated with or without RA were analyzed using qRT-PCR at the indicated time points ( n = 4). Results are expressed as the mean ± SEM. * p
    Anti Vpac1 Vipr1 Extracellular Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti vpac1 vipr1 extracellular antibody/product/Alomone Labs
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    anti vpac1 vipr1 extracellular antibody - by Bioz Stars, 2022-08
    93/100 stars

    Images

    1) Product Images from "3D Organoid Culture From Adult Salivary Gland Tissues as an ex vivo Modeling of Salivary Gland Morphogenesis"

    Article Title: 3D Organoid Culture From Adult Salivary Gland Tissues as an ex vivo Modeling of Salivary Gland Morphogenesis

    Journal: Frontiers in Cell and Developmental Biology

    doi: 10.3389/fcell.2021.698292

    Retinoic acid induces Vipr1 gene expression. (A) SMG organoids were treated with or without RA (300 nM) for the indicated time. After harvesting, the organoids were subjected to qRT-PCR to evaluate gene expression associated with VIP signaling ( n = 4). (B) SMG organoids were treated with varying doses of RA for 7 days and subjected to qRT-PCR for the evaluation of gene expression of Vipr1 and Rarb , a positive control, for RA-mediated signaling ( n = 4). (C) Expression of VIPR1 (green) in mouse SMG tissues (left) was assessed by co-staining with KRT5 (magenta, left), KRT7 (cyan, left), ACTA2 (magenta, right), and MIST1 (cyan, right). Nuclei were counterstained with DAPI (white) and each single channel image was placed below. Scale bars indicate 20 μm. (D) The mouse SMG organoids cultured with (bottom) or without RA (top) were subjected to immunofluorescence for the evaluation of VIPR1 (green), KRT5 (magenta), and KRT7 (cyan) expressions. Nuclei were counterstained with DAPI (white), and each single channel image was placed below. Scale bars indicate 50 μm. (E) The expression of several salivary gland markers in organoids treated with or without RA were analyzed using qRT-PCR at the indicated time points ( n = 4). Results are expressed as the mean ± SEM. * p
    Figure Legend Snippet: Retinoic acid induces Vipr1 gene expression. (A) SMG organoids were treated with or without RA (300 nM) for the indicated time. After harvesting, the organoids were subjected to qRT-PCR to evaluate gene expression associated with VIP signaling ( n = 4). (B) SMG organoids were treated with varying doses of RA for 7 days and subjected to qRT-PCR for the evaluation of gene expression of Vipr1 and Rarb , a positive control, for RA-mediated signaling ( n = 4). (C) Expression of VIPR1 (green) in mouse SMG tissues (left) was assessed by co-staining with KRT5 (magenta, left), KRT7 (cyan, left), ACTA2 (magenta, right), and MIST1 (cyan, right). Nuclei were counterstained with DAPI (white) and each single channel image was placed below. Scale bars indicate 20 μm. (D) The mouse SMG organoids cultured with (bottom) or without RA (top) were subjected to immunofluorescence for the evaluation of VIPR1 (green), KRT5 (magenta), and KRT7 (cyan) expressions. Nuclei were counterstained with DAPI (white), and each single channel image was placed below. Scale bars indicate 50 μm. (E) The expression of several salivary gland markers in organoids treated with or without RA were analyzed using qRT-PCR at the indicated time points ( n = 4). Results are expressed as the mean ± SEM. * p

    Techniques Used: Expressing, Quantitative RT-PCR, Positive Control, Staining, Cell Culture, Immunofluorescence

    RAR activation induces lumen formation in SMG organoids. (A) SMG organoids were cultured with 300 nM RA, TTNPB (RAR agonist), or bexarotene (RXR agonist) for 7 days and subjected to qRT-PCR for the detection of Vipr1 and Krt7 expression ( n = 4). (B) SMG organoids were treated with RA (300 nM), RA + 3 μM AGN-193109 (RAR antagonist), or RA + 3 μM HX (HX-531, RXR antagonist) throughout the culturing period and subjected to qRT-PCR for the detection of Vipr1 and Krt7 expression ( n = 4). (C–D) SMG organoids were treated with RA (300 nM), AGN-193109 (3 μM), or both. The organoids were treated with VIP (200 nM) on day 2 for lumen formation. After 3 days, lumen formation was observed via microscopic images (C) . Black arrows indicate organoids containing visible lumen. The proportion of organoids with lumen were calculated and statistically analyzed (D) ( n = 3). Scale bars indicate 100 μm in (C) . (E) SMG organoids were treated with 50μM z-VAD-FMK, a pan-caspase inhibitor, for 2 days, followed by whole-mount immunostaining with antibodies against CC3 (cleaved caspase-3) and TJP1 (ZO-1). DAPI (blue) was used for counterstaining the nucleus. Scale bars indicate 20 μm. (F) Apoptotic cell death (Annexin-V + PI – ) in SMG organoids treatedwith or without zVAD was examined using flow cytometry. (G) Representative bright-field images of organoids with lumen were obtained on day 5. Black arrows indicate organoids containing visible lumen. Scale bars indicate 100 μm. (H) The proportion of organoids with lumen were calculated ( n = 3). Results are expressed as the mean ± SEM. * p
    Figure Legend Snippet: RAR activation induces lumen formation in SMG organoids. (A) SMG organoids were cultured with 300 nM RA, TTNPB (RAR agonist), or bexarotene (RXR agonist) for 7 days and subjected to qRT-PCR for the detection of Vipr1 and Krt7 expression ( n = 4). (B) SMG organoids were treated with RA (300 nM), RA + 3 μM AGN-193109 (RAR antagonist), or RA + 3 μM HX (HX-531, RXR antagonist) throughout the culturing period and subjected to qRT-PCR for the detection of Vipr1 and Krt7 expression ( n = 4). (C–D) SMG organoids were treated with RA (300 nM), AGN-193109 (3 μM), or both. The organoids were treated with VIP (200 nM) on day 2 for lumen formation. After 3 days, lumen formation was observed via microscopic images (C) . Black arrows indicate organoids containing visible lumen. The proportion of organoids with lumen were calculated and statistically analyzed (D) ( n = 3). Scale bars indicate 100 μm in (C) . (E) SMG organoids were treated with 50μM z-VAD-FMK, a pan-caspase inhibitor, for 2 days, followed by whole-mount immunostaining with antibodies against CC3 (cleaved caspase-3) and TJP1 (ZO-1). DAPI (blue) was used for counterstaining the nucleus. Scale bars indicate 20 μm. (F) Apoptotic cell death (Annexin-V + PI – ) in SMG organoids treatedwith or without zVAD was examined using flow cytometry. (G) Representative bright-field images of organoids with lumen were obtained on day 5. Black arrows indicate organoids containing visible lumen. Scale bars indicate 100 μm. (H) The proportion of organoids with lumen were calculated ( n = 3). Results are expressed as the mean ± SEM. * p

    Techniques Used: Activation Assay, Cell Culture, Quantitative RT-PCR, Expressing, Immunostaining, Flow Cytometry

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    Alomone Labs anti vpac1 vipr1 extracellular antibody
    Retinoic acid induces <t>Vipr1</t> gene expression. (A) SMG organoids were treated with or without RA (300 nM) for the indicated time. After harvesting, the organoids were subjected to qRT-PCR to evaluate gene expression associated with VIP signaling ( n = 4). (B) SMG organoids were treated with varying doses of RA for 7 days and subjected to qRT-PCR for the evaluation of gene expression of Vipr1 and Rarb , a positive control, for RA-mediated signaling ( n = 4). (C) Expression of VIPR1 (green) in mouse SMG tissues (left) was assessed by co-staining with KRT5 (magenta, left), KRT7 (cyan, left), ACTA2 (magenta, right), and MIST1 (cyan, right). Nuclei were counterstained with DAPI (white) and each single channel image was placed below. Scale bars indicate 20 μm. (D) The mouse SMG organoids cultured with (bottom) or without RA (top) were subjected to immunofluorescence for the evaluation of VIPR1 (green), KRT5 (magenta), and KRT7 (cyan) expressions. Nuclei were counterstained with DAPI (white), and each single channel image was placed below. Scale bars indicate 50 μm. (E) The expression of several salivary gland markers in organoids treated with or without RA were analyzed using qRT-PCR at the indicated time points ( n = 4). Results are expressed as the mean ± SEM. * p
    Anti Vpac1 Vipr1 Extracellular Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti vpac1 vipr1 extracellular antibody/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti vpac1 vipr1 extracellular antibody - by Bioz Stars, 2022-08
    93/100 stars
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    Retinoic acid induces Vipr1 gene expression. (A) SMG organoids were treated with or without RA (300 nM) for the indicated time. After harvesting, the organoids were subjected to qRT-PCR to evaluate gene expression associated with VIP signaling ( n = 4). (B) SMG organoids were treated with varying doses of RA for 7 days and subjected to qRT-PCR for the evaluation of gene expression of Vipr1 and Rarb , a positive control, for RA-mediated signaling ( n = 4). (C) Expression of VIPR1 (green) in mouse SMG tissues (left) was assessed by co-staining with KRT5 (magenta, left), KRT7 (cyan, left), ACTA2 (magenta, right), and MIST1 (cyan, right). Nuclei were counterstained with DAPI (white) and each single channel image was placed below. Scale bars indicate 20 μm. (D) The mouse SMG organoids cultured with (bottom) or without RA (top) were subjected to immunofluorescence for the evaluation of VIPR1 (green), KRT5 (magenta), and KRT7 (cyan) expressions. Nuclei were counterstained with DAPI (white), and each single channel image was placed below. Scale bars indicate 50 μm. (E) The expression of several salivary gland markers in organoids treated with or without RA were analyzed using qRT-PCR at the indicated time points ( n = 4). Results are expressed as the mean ± SEM. * p

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: 3D Organoid Culture From Adult Salivary Gland Tissues as an ex vivo Modeling of Salivary Gland Morphogenesis

    doi: 10.3389/fcell.2021.698292

    Figure Lengend Snippet: Retinoic acid induces Vipr1 gene expression. (A) SMG organoids were treated with or without RA (300 nM) for the indicated time. After harvesting, the organoids were subjected to qRT-PCR to evaluate gene expression associated with VIP signaling ( n = 4). (B) SMG organoids were treated with varying doses of RA for 7 days and subjected to qRT-PCR for the evaluation of gene expression of Vipr1 and Rarb , a positive control, for RA-mediated signaling ( n = 4). (C) Expression of VIPR1 (green) in mouse SMG tissues (left) was assessed by co-staining with KRT5 (magenta, left), KRT7 (cyan, left), ACTA2 (magenta, right), and MIST1 (cyan, right). Nuclei were counterstained with DAPI (white) and each single channel image was placed below. Scale bars indicate 20 μm. (D) The mouse SMG organoids cultured with (bottom) or without RA (top) were subjected to immunofluorescence for the evaluation of VIPR1 (green), KRT5 (magenta), and KRT7 (cyan) expressions. Nuclei were counterstained with DAPI (white), and each single channel image was placed below. Scale bars indicate 50 μm. (E) The expression of several salivary gland markers in organoids treated with or without RA were analyzed using qRT-PCR at the indicated time points ( n = 4). Results are expressed as the mean ± SEM. * p

    Article Snippet: The sections or organoids were incubated with primary antibodies overnight at 4°C as follows: chicken anti-KRT5 (905901, BioLegend, 1:1,000); mouse anti-KRT7 (ab9021, Abcam, 1:200); rabbit anti-KRT-19 (ab52625, Abcam, 1:500); rabbit anti-CD133 (orb99113, Biorbyt, 1:50); rabbit anti-VIPR1 (AVR-001, Alomone, 1:100); mouse anti-MIST1 (ab110919, Abcam, 1:50), goat anti-ACTA2 (NB300-978, Novus Bio, 1:1,000), rabbit anti-cleaved Caspase-3 (9661s, CST, 1:500), and goat anti-TJP1 (ABIN6254231, antibodies-online.

    Techniques: Expressing, Quantitative RT-PCR, Positive Control, Staining, Cell Culture, Immunofluorescence

    RAR activation induces lumen formation in SMG organoids. (A) SMG organoids were cultured with 300 nM RA, TTNPB (RAR agonist), or bexarotene (RXR agonist) for 7 days and subjected to qRT-PCR for the detection of Vipr1 and Krt7 expression ( n = 4). (B) SMG organoids were treated with RA (300 nM), RA + 3 μM AGN-193109 (RAR antagonist), or RA + 3 μM HX (HX-531, RXR antagonist) throughout the culturing period and subjected to qRT-PCR for the detection of Vipr1 and Krt7 expression ( n = 4). (C–D) SMG organoids were treated with RA (300 nM), AGN-193109 (3 μM), or both. The organoids were treated with VIP (200 nM) on day 2 for lumen formation. After 3 days, lumen formation was observed via microscopic images (C) . Black arrows indicate organoids containing visible lumen. The proportion of organoids with lumen were calculated and statistically analyzed (D) ( n = 3). Scale bars indicate 100 μm in (C) . (E) SMG organoids were treated with 50μM z-VAD-FMK, a pan-caspase inhibitor, for 2 days, followed by whole-mount immunostaining with antibodies against CC3 (cleaved caspase-3) and TJP1 (ZO-1). DAPI (blue) was used for counterstaining the nucleus. Scale bars indicate 20 μm. (F) Apoptotic cell death (Annexin-V + PI – ) in SMG organoids treatedwith or without zVAD was examined using flow cytometry. (G) Representative bright-field images of organoids with lumen were obtained on day 5. Black arrows indicate organoids containing visible lumen. Scale bars indicate 100 μm. (H) The proportion of organoids with lumen were calculated ( n = 3). Results are expressed as the mean ± SEM. * p

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: 3D Organoid Culture From Adult Salivary Gland Tissues as an ex vivo Modeling of Salivary Gland Morphogenesis

    doi: 10.3389/fcell.2021.698292

    Figure Lengend Snippet: RAR activation induces lumen formation in SMG organoids. (A) SMG organoids were cultured with 300 nM RA, TTNPB (RAR agonist), or bexarotene (RXR agonist) for 7 days and subjected to qRT-PCR for the detection of Vipr1 and Krt7 expression ( n = 4). (B) SMG organoids were treated with RA (300 nM), RA + 3 μM AGN-193109 (RAR antagonist), or RA + 3 μM HX (HX-531, RXR antagonist) throughout the culturing period and subjected to qRT-PCR for the detection of Vipr1 and Krt7 expression ( n = 4). (C–D) SMG organoids were treated with RA (300 nM), AGN-193109 (3 μM), or both. The organoids were treated with VIP (200 nM) on day 2 for lumen formation. After 3 days, lumen formation was observed via microscopic images (C) . Black arrows indicate organoids containing visible lumen. The proportion of organoids with lumen were calculated and statistically analyzed (D) ( n = 3). Scale bars indicate 100 μm in (C) . (E) SMG organoids were treated with 50μM z-VAD-FMK, a pan-caspase inhibitor, for 2 days, followed by whole-mount immunostaining with antibodies against CC3 (cleaved caspase-3) and TJP1 (ZO-1). DAPI (blue) was used for counterstaining the nucleus. Scale bars indicate 20 μm. (F) Apoptotic cell death (Annexin-V + PI – ) in SMG organoids treatedwith or without zVAD was examined using flow cytometry. (G) Representative bright-field images of organoids with lumen were obtained on day 5. Black arrows indicate organoids containing visible lumen. Scale bars indicate 100 μm. (H) The proportion of organoids with lumen were calculated ( n = 3). Results are expressed as the mean ± SEM. * p

    Article Snippet: The sections or organoids were incubated with primary antibodies overnight at 4°C as follows: chicken anti-KRT5 (905901, BioLegend, 1:1,000); mouse anti-KRT7 (ab9021, Abcam, 1:200); rabbit anti-KRT-19 (ab52625, Abcam, 1:500); rabbit anti-CD133 (orb99113, Biorbyt, 1:50); rabbit anti-VIPR1 (AVR-001, Alomone, 1:100); mouse anti-MIST1 (ab110919, Abcam, 1:50), goat anti-ACTA2 (NB300-978, Novus Bio, 1:1,000), rabbit anti-cleaved Caspase-3 (9661s, CST, 1:500), and goat anti-TJP1 (ABIN6254231, antibodies-online.

    Techniques: Activation Assay, Cell Culture, Quantitative RT-PCR, Expressing, Immunostaining, Flow Cytometry