ht3br asr032  (Alomone Labs)

Journal: Scientific Reports

Article Title: Sexual dimorphic impacts of systemic vincristine on lower urinary tract function

doi: 10.1038/s41598-022-08585-3

Figure Lengend Snippet: Antibodies.

Article Snippet: Htr3b , Alomone Labs (Jerusalem, Israel), ASR-032 , 1:100 (IF), 1:500 (WB) , Rabbit , Fig. A .

Techniques:

Journal: Scientific Reports

Article Title: Sexual dimorphic impacts of systemic vincristine on lower urinary tract function

doi: 10.1038/s41598-022-08585-3

Figure Lengend Snippet: Immunofluorescence labeling of the bladders. Representative immunohistochemical images with antibodies against Htr3b (green, a and d ), Tubb3 (red, b and e ), and merged image ( c and f ) along nuclei staining using DAPI (blue) on the bladder sections from female mice in the control ( a – c ) and VCR ( d – f ) groups. Arrowheads indicate the cluster-forming Tubb3 IR. Representative immunohistochemical images on the bladder sections from male mice in the control ( g – i and m – o ) and VCR ( j – l and p – r ) groups with antibodies against Itga1 (green, g and j ), Tubb3 (red, h and k ), Desmin (green, m and p ), Trpa1 (red, n and q ) and merged image ( i , l , o , and r ) along nuclei staining using DAPI (blue). Scale bars = 250 µm. Figures were prepared using Adobe Photoshop CS6.

Article Snippet: Htr3b , Alomone Labs (Jerusalem, Israel), ASR-032 , 1:100 (IF), 1:500 (WB) , Rabbit , Fig. A .

Techniques: Immunofluorescence, Labeling, Immunohistochemical staining, Staining

Journal: Scientific Reports

Article Title: Sexual dimorphic impacts of systemic vincristine on lower urinary tract function

doi: 10.1038/s41598-022-08585-3

Figure Lengend Snippet: Schematic diagram of the proposed mechanisms underlying vincristine induced alterations in lower urinary tract function. Systemic VCR exposure induces deficiency in bladder sensation, leading to a decrease in micturition frequency accompanied with an increased bladder capacity. In addition to the VCR-induced impairments of axonal transport and distal axonopathy, sexual dimorphic mechanisms contribute to the phenotype; in females, a decrease in bladder afferent firing through downregulation of Htr3b signaling while in males, neuroinflammation. Syndrome of inappropriate secretion of antidiuretic hormone (SIADH) can exacerbate the symptoms. In males, the dysregulations of cellular ion homeostasis in the bladder through enhanced Trpa1 signaling induce increases in cell excitability, leading to detrusor overactivity. The VCR-induced neuroinflammation also can play a role in detrusor overactivity in male. Systemic VCR exposure can increase risk of developing long-term sex specific LUTD via multiple mechanisms.

Article Snippet: Htr3b , Alomone Labs (Jerusalem, Israel), ASR-032 , 1:100 (IF), 1:500 (WB) , Rabbit , Fig. A .

Techniques: