unconjugated anti s1pr3 antibody  (Alomone Labs)


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    Alomone Labs unconjugated anti s1pr3 antibody
    On-site delivery of FTY720 recruits blood-derived non-classical monocytes and increases the frequency of alternatively activated macrophages. PLGA films loaded with FTY720 were implanted immediately after DWC surgery. ( a ) Proportion of latex bead-positive (LX+) Ly6C lo monocytes that were derived from blood in tissue 3 days post-injury following labeling of Ly6C lo monocytes. ( b ) Overall frequency of CD206+ F4/80+CD11b+ macrophages in the presence of FTY720. ( c ) LX+ cells in tissue surrounding unloaded or FTY720-loaded PLGA implants. ( d ) Proportion of LX+ Ly6C hi monocytes that were derived from blood following labeling of Ly6C hi monocytes with Clod Lip. ( e ) Overall frequency of CD206+ F4/80+CD11b+ macrophages in the presence of FTY720 after Clod Lip administration. ( f ) LX+ cells in tissue surrounding unloaded or FTY720-loaded PLGA implants. ( g ) Ly6C expression in CX3CR1 hi monocytes and CX3CR1 lo monocytes obtained from the blood of CX3CR1 GFP/+ mice identify Ly6C lo monocytes and Ly6C hi monocytes, respectively. ( h,i ) Surface <t>S1PR3</t> expression in blood CX3CR1 hi cells and CX3CR1 lo cells. To control for background staining, CX3CR1 GFP/+ cells were stained with secondary antibody (2° Ab) only, which is shown in the gray histogram. Scale bars, 500 µm. Data presented as mean ± S.E.M. Statistical analyses were performed using two-tailed t-tests. *p < 0.05, n = 4–10 animals per group.
    Unconjugated Anti S1pr3 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/unconjugated anti s1pr3 antibody/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    unconjugated anti s1pr3 antibody - by Bioz Stars, 2023-10
    86/100 stars

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    1) Product Images from "Non-classical monocytes are biased progenitors of wound healing macrophages during soft tissue injury"

    Article Title: Non-classical monocytes are biased progenitors of wound healing macrophages during soft tissue injury

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-00477-1

    On-site delivery of FTY720 recruits blood-derived non-classical monocytes and increases the frequency of alternatively activated macrophages. PLGA films loaded with FTY720 were implanted immediately after DWC surgery. ( a ) Proportion of latex bead-positive (LX+) Ly6C lo monocytes that were derived from blood in tissue 3 days post-injury following labeling of Ly6C lo monocytes. ( b ) Overall frequency of CD206+ F4/80+CD11b+ macrophages in the presence of FTY720. ( c ) LX+ cells in tissue surrounding unloaded or FTY720-loaded PLGA implants. ( d ) Proportion of LX+ Ly6C hi monocytes that were derived from blood following labeling of Ly6C hi monocytes with Clod Lip. ( e ) Overall frequency of CD206+ F4/80+CD11b+ macrophages in the presence of FTY720 after Clod Lip administration. ( f ) LX+ cells in tissue surrounding unloaded or FTY720-loaded PLGA implants. ( g ) Ly6C expression in CX3CR1 hi monocytes and CX3CR1 lo monocytes obtained from the blood of CX3CR1 GFP/+ mice identify Ly6C lo monocytes and Ly6C hi monocytes, respectively. ( h,i ) Surface S1PR3 expression in blood CX3CR1 hi cells and CX3CR1 lo cells. To control for background staining, CX3CR1 GFP/+ cells were stained with secondary antibody (2° Ab) only, which is shown in the gray histogram. Scale bars, 500 µm. Data presented as mean ± S.E.M. Statistical analyses were performed using two-tailed t-tests. *p < 0.05, n = 4–10 animals per group.
    Figure Legend Snippet: On-site delivery of FTY720 recruits blood-derived non-classical monocytes and increases the frequency of alternatively activated macrophages. PLGA films loaded with FTY720 were implanted immediately after DWC surgery. ( a ) Proportion of latex bead-positive (LX+) Ly6C lo monocytes that were derived from blood in tissue 3 days post-injury following labeling of Ly6C lo monocytes. ( b ) Overall frequency of CD206+ F4/80+CD11b+ macrophages in the presence of FTY720. ( c ) LX+ cells in tissue surrounding unloaded or FTY720-loaded PLGA implants. ( d ) Proportion of LX+ Ly6C hi monocytes that were derived from blood following labeling of Ly6C hi monocytes with Clod Lip. ( e ) Overall frequency of CD206+ F4/80+CD11b+ macrophages in the presence of FTY720 after Clod Lip administration. ( f ) LX+ cells in tissue surrounding unloaded or FTY720-loaded PLGA implants. ( g ) Ly6C expression in CX3CR1 hi monocytes and CX3CR1 lo monocytes obtained from the blood of CX3CR1 GFP/+ mice identify Ly6C lo monocytes and Ly6C hi monocytes, respectively. ( h,i ) Surface S1PR3 expression in blood CX3CR1 hi cells and CX3CR1 lo cells. To control for background staining, CX3CR1 GFP/+ cells were stained with secondary antibody (2° Ab) only, which is shown in the gray histogram. Scale bars, 500 µm. Data presented as mean ± S.E.M. Statistical analyses were performed using two-tailed t-tests. *p < 0.05, n = 4–10 animals per group.

    Techniques Used: Derivative Assay, Labeling, Expressing, Staining, Two Tailed Test

    Local FTY720 release recruits biased progenitors of wound healing macrophages to inflamed tissue. Circulating non-classical S1PR3 hi monocytes are recruited by local delivery of FTY720 from a material implanted within an injury site. Upon entrance into inflamed tissue, non-classical monocytes give rise to alternatively activated, wound healing macrophages. Conversely, classical monocytes differentiate into both inflammatory and wound healing macrophages.
    Figure Legend Snippet: Local FTY720 release recruits biased progenitors of wound healing macrophages to inflamed tissue. Circulating non-classical S1PR3 hi monocytes are recruited by local delivery of FTY720 from a material implanted within an injury site. Upon entrance into inflamed tissue, non-classical monocytes give rise to alternatively activated, wound healing macrophages. Conversely, classical monocytes differentiate into both inflammatory and wound healing macrophages.

    Techniques Used:

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    • unconjugated anti s1pr3 antibody  (Alomone Labs)
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    Alomone Labs unconjugated anti s1pr3 antibody
    On-site delivery of FTY720 recruits blood-derived non-classical monocytes and increases the frequency of alternatively activated macrophages. PLGA films loaded with FTY720 were implanted immediately after DWC surgery. ( a ) Proportion of latex bead-positive (LX+) Ly6C lo monocytes that were derived from blood in tissue 3 days post-injury following labeling of Ly6C lo monocytes. ( b ) Overall frequency of CD206+ F4/80+CD11b+ macrophages in the presence of FTY720. ( c ) LX+ cells in tissue surrounding unloaded or FTY720-loaded PLGA implants. ( d ) Proportion of LX+ Ly6C hi monocytes that were derived from blood following labeling of Ly6C hi monocytes with Clod Lip. ( e ) Overall frequency of CD206+ F4/80+CD11b+ macrophages in the presence of FTY720 after Clod Lip administration. ( f ) LX+ cells in tissue surrounding unloaded or FTY720-loaded PLGA implants. ( g ) Ly6C expression in CX3CR1 hi monocytes and CX3CR1 lo monocytes obtained from the blood of CX3CR1 GFP/+ mice identify Ly6C lo monocytes and Ly6C hi monocytes, respectively. ( h,i ) Surface <t>S1PR3</t> expression in blood CX3CR1 hi cells and CX3CR1 lo cells. To control for background staining, CX3CR1 GFP/+ cells were stained with secondary antibody (2° Ab) only, which is shown in the gray histogram. Scale bars, 500 µm. Data presented as mean ± S.E.M. Statistical analyses were performed using two-tailed t-tests. *p < 0.05, n = 4–10 animals per group.
    Unconjugated Anti S1pr3 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/unconjugated anti s1pr3 antibody/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    unconjugated anti s1pr3 antibody - by Bioz Stars, 2023-10
    86/100 stars
    		  Buy from Supplier

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    On-site delivery of FTY720 recruits blood-derived non-classical monocytes and increases the frequency of alternatively activated macrophages. PLGA films loaded with FTY720 were implanted immediately after DWC surgery. ( a ) Proportion of latex bead-positive (LX+) Ly6C lo monocytes that were derived from blood in tissue 3 days post-injury following labeling of Ly6C lo monocytes. ( b ) Overall frequency of CD206+ F4/80+CD11b+ macrophages in the presence of FTY720. ( c ) LX+ cells in tissue surrounding unloaded or FTY720-loaded PLGA implants. ( d ) Proportion of LX+ Ly6C hi monocytes that were derived from blood following labeling of Ly6C hi monocytes with Clod Lip. ( e ) Overall frequency of CD206+ F4/80+CD11b+ macrophages in the presence of FTY720 after Clod Lip administration. ( f ) LX+ cells in tissue surrounding unloaded or FTY720-loaded PLGA implants. ( g ) Ly6C expression in CX3CR1 hi monocytes and CX3CR1 lo monocytes obtained from the blood of CX3CR1 GFP/+ mice identify Ly6C lo monocytes and Ly6C hi monocytes, respectively. ( h,i ) Surface S1PR3 expression in blood CX3CR1 hi cells and CX3CR1 lo cells. To control for background staining, CX3CR1 GFP/+ cells were stained with secondary antibody (2° Ab) only, which is shown in the gray histogram. Scale bars, 500 µm. Data presented as mean ± S.E.M. Statistical analyses were performed using two-tailed t-tests. *p < 0.05, n = 4–10 animals per group.

    Journal: Scientific Reports

    Article Title: Non-classical monocytes are biased progenitors of wound healing macrophages during soft tissue injury

    doi: 10.1038/s41598-017-00477-1

    Figure Lengend Snippet: On-site delivery of FTY720 recruits blood-derived non-classical monocytes and increases the frequency of alternatively activated macrophages. PLGA films loaded with FTY720 were implanted immediately after DWC surgery. ( a ) Proportion of latex bead-positive (LX+) Ly6C lo monocytes that were derived from blood in tissue 3 days post-injury following labeling of Ly6C lo monocytes. ( b ) Overall frequency of CD206+ F4/80+CD11b+ macrophages in the presence of FTY720. ( c ) LX+ cells in tissue surrounding unloaded or FTY720-loaded PLGA implants. ( d ) Proportion of LX+ Ly6C hi monocytes that were derived from blood following labeling of Ly6C hi monocytes with Clod Lip. ( e ) Overall frequency of CD206+ F4/80+CD11b+ macrophages in the presence of FTY720 after Clod Lip administration. ( f ) LX+ cells in tissue surrounding unloaded or FTY720-loaded PLGA implants. ( g ) Ly6C expression in CX3CR1 hi monocytes and CX3CR1 lo monocytes obtained from the blood of CX3CR1 GFP/+ mice identify Ly6C lo monocytes and Ly6C hi monocytes, respectively. ( h,i ) Surface S1PR3 expression in blood CX3CR1 hi cells and CX3CR1 lo cells. To control for background staining, CX3CR1 GFP/+ cells were stained with secondary antibody (2° Ab) only, which is shown in the gray histogram. Scale bars, 500 µm. Data presented as mean ± S.E.M. Statistical analyses were performed using two-tailed t-tests. *p < 0.05, n = 4–10 animals per group.

    Article Snippet: S1PR3 flow cytometry was performed by first performing Fc block (Biolegend), followed by staining cells with primary unconjugated anti-S1PR3 antibody (Alomone Labs) and secondary staining with DyLight 650 anti-rabbit IgG (Abcam).

    Techniques: Derivative Assay, Labeling, Expressing, Staining, Two Tailed Test

    Local FTY720 release recruits biased progenitors of wound healing macrophages to inflamed tissue. Circulating non-classical S1PR3 hi monocytes are recruited by local delivery of FTY720 from a material implanted within an injury site. Upon entrance into inflamed tissue, non-classical monocytes give rise to alternatively activated, wound healing macrophages. Conversely, classical monocytes differentiate into both inflammatory and wound healing macrophages.

    Journal: Scientific Reports

    Article Title: Non-classical monocytes are biased progenitors of wound healing macrophages during soft tissue injury

    doi: 10.1038/s41598-017-00477-1

    Figure Lengend Snippet: Local FTY720 release recruits biased progenitors of wound healing macrophages to inflamed tissue. Circulating non-classical S1PR3 hi monocytes are recruited by local delivery of FTY720 from a material implanted within an injury site. Upon entrance into inflamed tissue, non-classical monocytes give rise to alternatively activated, wound healing macrophages. Conversely, classical monocytes differentiate into both inflammatory and wound healing macrophages.

    Article Snippet: S1PR3 flow cytometry was performed by first performing Fc block (Biolegend), followed by staining cells with primary unconjugated anti-S1PR3 antibody (Alomone Labs) and secondary staining with DyLight 650 anti-rabbit IgG (Abcam).

    Techniques: