Rabbit Anti S1pr1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Sphingosine 1-Phosphate Receptor 1 Modulates CNTF-Induced Axonal Growth and Neuroprotection in the Mouse Visual System"
Article Title: Sphingosine 1-Phosphate Receptor 1 Modulates CNTF-Induced Axonal Growth and Neuroprotection in the Mouse Visual System
Journal: Neural Plasticity
Figure Legend Snippet: Modulation of S1PR1 expression by the CNTF/Stat3 pathway in optic nerve-injured retinae. (a, b) S1PR1 expression changes were monitored 3 d after ONC in retinae infected with ShH10.CNTF or ShH10.Empty, a control vector that was deprived of cDNA sequence. ShH10 viruses preferentially infected Müller glia in the retina [ 16 ]. P-Stat3 and S1PR1 were markedly increased by ShH10.CNTF in RGC somata identified using β 3-tubulin as a specific marker. (c) Five days after ONC, qRT-PCR measurements showed that the infection of RGCs with AAV2.Stat3 significantly increased the mRNA level of S1pr1 compared with control AAV2.GFP vector. ShH10 and AAV2 viruses were intravitreally injected 4 weeks before ONC. Three mice were analyzed/grouped. Statistics: one-way ANOVA; ∗ p
Techniques Used: Expressing, Infection, Plasmid Preparation, Sequencing, Marker, Quantitative RT-PCR, Injection, Mouse Assay
Figure Legend Snippet: S1PR1 knockdown alters CNTF-induced RGC survival after ONC. (a) Two weeks after ONC, surviving RGCs were observed in retinal flat-mounts after immunofluorescent staining for β 3-tubulin. Less RGCs were visible in retinae infected with AAV2.shRNA-S1PR1 and ShH10.CNTF ( n = 5 mice) than in mice injected with ShH10.CNTF/AAV2.GFP ( n = 7 mice) or ShH10.CNTF/AAV2.shRNA-S1PR1 ( n = 5 mice). (b) Quantitatively, the average number of surviving RGCs was statistically lower in whole retinae transduced with ShH10.CNTF/AAV2.shRNA-S1PR1 than in the two other groups of animals (ANOVA, ∗∗∗ p
Techniques Used: Staining, Infection, shRNA, Mouse Assay, Injection, Transduction
Figure Legend Snippet: The expression of P-Stat3 is not changed by S1PR1 silencing after CNTF stimulation. The expression of P-Stat3 was assessed by western blotting in protein lysates (20 μ g) from retinae treated with ShH10 and AAV2 viruses. P-Stat3 and Stat3 blots were quantified by densitometry using the ImageJ software (NIH). The level of P-Stat3/Stat3 was not significantly different between mice treated with ShH10.CNTF/AAV2.shRNA-S1PR1 and ShH10.CNTF/AAV2.GFP. Three mice were analyzed for each group.
Techniques Used: Expressing, Western Blot, Software, Mouse Assay, shRNA
Figure Legend Snippet: Hypothetical mechanism by which CNTF/Stat3 and S1P/S1PR1 interaction may orchestrate neuronal survival and axonal growth. CNTF binds and activates a heterotrimeric receptor complex, composed of CNTFR α , leukemia inhibitory factor receptor (LIFR), and gp130, leading to Stat3 phosphorylation (P-Stat3) and activation. (a) P-Stat3-driven transcription may increase the expression of S1PR1 and its translocation to the plasma membrane. The activation of S1PR1 by S1P may trigger downstream growth mechanisms resulting in (b) neuronal survival and (c) axonal growth.
Techniques Used: Activation Assay, Expressing, Translocation Assay
Figure Legend Snippet: S1PR1 knockdown potentiates CNTF-induced axonal regeneration. (a) Axonal regeneration was visualized on longitudinal sections of optic nerves two weeks after crush injury and 4 weeks after coinfection with ShH10.CNTF and AAV2 vectors. Axons were traced with cholera toxin β subunit (CTb) conjugated to Alexa 594 the day before tissue fixation. (b) The infection of retinal cells with ShH10.CNTF and AAV2.shRNA-S1PR1 promoted lengthy axonal regeneration in the optic nerve compared with the ShH10.CNTF/AAV2.GFP combination. (c) Quantitatively, axonal fibers were significantly more numerous between 1300 and 1800 μ m past the lesion site with ShH10.CNTF/AAV2.shRNA-S1PR1 ( n = 6 mice) than with ShH10.CNTF/AAV2.GFP ( n = 5 mice) treatments (ANOVA, ∗ p
Techniques Used: CtB Assay, Infection, shRNA, Mouse Assay
2) Product Images from "Differential sphingosine-1-phosphate receptor-1 (S1PR1) protein expressions in the dorsolateral prefrontal cortex between schizophrenia Type 1 and Type 2"
Article Title: Differential sphingosine-1-phosphate receptor-1 (S1PR1) protein expressions in the dorsolateral prefrontal cortex between schizophrenia Type 1 and Type 2
Figure Legend Snippet: Autoradiograph analysis of S1PR1 using S1PR1 specific [ 3 H]CS1P1 in control and schizophrenia DLPFC. A) Comparison among control, schizophrenia Type 1 and schizophrenia Type 2 (* represents p
Techniques Used: Autoradiography
Figure Legend Snippet: Representative images of autoradiograph analysis of S1PR1 using S1PR1 specific [ 3 H]CS1P1 in control and schizophrenia DLPFC.
Techniques Used: Autoradiography
Figure Legend Snippet: Immunohistochemistry of S1PR1 in control and schizophrenia DLPFC.
Techniques Used: Immunohistochemistry