Asic1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Acid-sensing ion channel 1a contributes to the effect of extracellular acidosis on NLRP1 inflammasome activation in cortical neurons"
Article Title: Acid-sensing ion channel 1a contributes to the effect of extracellular acidosis on NLRP1 inflammasome activation in cortical neurons
Journal: Journal of Neuroinflammation
Figure Legend Snippet: ASICs and BK channels are co-expressed in cortical neurons. Detection of the expression of ASICs and BK channels in cortical neurons by double-staining immunofluorescence (original magnification ×400). Nuclei were counterstained with Hoechst33258 ( blue ). ASICs and BK channels were labeled with FITC ( green ) and TRITC ( red ). There is a co-expression between ASIC1, ASIC2, ASIC3, and BK channels
Techniques Used: Expressing, Double Immunofluorescence Staining, Labeling
2) Product Images from "Alveolar nonselective channels are ASIC1a/α-ENaC channels and contribute to AFC"
Article Title: Alveolar nonselective channels are ASIC1a/α-ENaC channels and contribute to AFC
Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology
Figure Legend Snippet: AT 2 cells contain ACCN2 . A : PCR analysis showing expression of ACCN isoforms in cDNA prepared from AT 2 cells. Only ACCN2 (ASIC1) has a visible band. B : PCR reamplification showing expression of ACCN2 variant 2 (ASIC1a, lane 3 ) and lack of expression of ACCN2 variant 1 (ASIC1b, lane 2 ). NCBI, National Center for Biotechnology Information.
Techniques Used: Polymerase Chain Reaction, Expressing, Variant Assay
Figure Legend Snippet: KO mice have no detectable ASIC1a. PCR analysis shows expression of ACCN2 in cDNA prepared from wild-type lungs and from rat lung as a positive control. The cDNA prepared from ASIC1 KO lung has no detectable ACCN2 (ASIC1).
Techniques Used: Mouse Assay, Polymerase Chain Reaction, Expressing, Positive Control
Figure Legend Snippet: NSC channel frequency strongly depends on ASIC1a and α-ENaC presence. A : Western blot showing ASIC1 expression in L2 cells treated with scrambled shRNA or ASIC1 silencing vectors. Although the blot is cropped, there are no other bands in the blot. Reduction of ASIC1 protein positively correlates to amount of ASIC1a shRNA used. B , left : percentage patches with NSC channels present under ASIC1 or α-ENaC knockdown conditions. Right : percentage patches with HSC channels under ASIC1 or α-ENaC knockdown conditions. Numbers above bars indicate total number of patches.
Techniques Used: Western Blot, Expressing, shRNA
Figure Legend Snippet: Interaction of ASIC1a and α-ENaC subunits. A , top : L2 cell protein lysate immunoprecipitated (IP) for α-ENaC and immunoblotted (IB) for ASIC1. Bottom : L2 cell protein lysate immunoprecipitated for ASIC1 and immunoblotted for α-ENaC. B : quantification of mammalian two-hybrid assay between ASIC1a and ENaC subunits. Normalized luciferase luminescence is proportional to binding affinity to ASIC1a. Negative control represents random association, and positive control represents maximum affinity. Fold increase for α-ENaC (6.6 ± 0.73) indicates a high affinity for ASIC1a. Data represent a total n of 15 ( n = 3 for each condition); * P
Techniques Used: Immunoprecipitation, Two Hybrid Assay, Luciferase, Binding Assay, Negative Control, Positive Control
Figure Legend Snippet: ASIC1a KO increases lung water content and reduces alveolar fluid clearance. A : lung wet wt-to-dry wt ratios. Higher wet wt-to-dry wt ratio indicates increased lung water content and decreased alveolar fluid clearance. The difference in the two groups is significant. Data represent n = 8 for each treatment group. B : Evans blue dye assay showed that alveolar fluid clearance was significantly reduced in ASIC1a KO mice compared with wild-type mice. Amiloride blocked about half of AFC in wild-type mice, but there is little residual AFC after amiloride in ASIC1 KO mice. Data represent a total n = 3 mice for each treatment group; n.s., not significant. C : bronchalveolar lavage (BAL) fluid protein from wild-type and ASIC1 KO mice. There is no significant difference in BAL protein between the two groups ( n = 4 mice for each treatment group; P = 0.424).
Techniques Used: Mouse Assay
Figure Legend Snippet: NSC channels are not observable in single-channel patches on lung slices from ASIC1 KO mice. Single-channel recordings were measured from AT 2 cells in lung slices. A : single-channel currents ( right ) and distribution of current amplitudes ( left ) in a patch on an AT 2 cell from a wild-type lung slice, which has both NSC and HSC channels (sometimes overlapping). B : single-channel currents ( right ) and distribution of current amplitudes ( left ) in a patch on an AT 2 cell from an ASIC1 KO lung slice, which has only HSC channels.
Techniques Used: Mouse Assay
Figure Legend Snippet: NSC channels are sensitive to ASIC1-modifying toxins. A : NSC channels were activated by venom of the Texas coral snake ( Micrurus tener tener ). B : psalmotoxin-1 isolated from the venom of the spider, Psalmopoeus cambridgei (Trinidad chevron tarantula), is a potent and selective acid-sensing ion channel 1a (ASIC1a) blocker (IC 50 ). When applied to AT 2 cells in primary culture, it uniformly decreased NSC open probability. Neither toxin had any effect on HSC channels. * P
Techniques Used: Isolation