asic1  (Alomone Labs)


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    Structured Review

    Alomone Labs asic1
    ASICs and BK channels are co-expressed in cortical neurons. Detection of the expression of ASICs and BK channels in cortical neurons by double-staining immunofluorescence (original magnification ×400). Nuclei were counterstained with Hoechst33258 ( blue ). ASICs and BK channels were labeled with FITC ( green ) and TRITC ( red ). There is a co-expression between <t>ASIC1,</t> ASIC2, ASIC3, and BK channels
    Asic1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/asic1/product/Alomone Labs
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    asic1 - by Bioz Stars, 2022-08
    93/100 stars

    Images

    1) Product Images from "Acid-sensing ion channel 1a contributes to the effect of extracellular acidosis on NLRP1 inflammasome activation in cortical neurons"

    Article Title: Acid-sensing ion channel 1a contributes to the effect of extracellular acidosis on NLRP1 inflammasome activation in cortical neurons

    Journal: Journal of Neuroinflammation

    doi: 10.1186/s12974-015-0465-7

    ASICs and BK channels are co-expressed in cortical neurons. Detection of the expression of ASICs and BK channels in cortical neurons by double-staining immunofluorescence (original magnification ×400). Nuclei were counterstained with Hoechst33258 ( blue ). ASICs and BK channels were labeled with FITC ( green ) and TRITC ( red ). There is a co-expression between ASIC1, ASIC2, ASIC3, and BK channels
    Figure Legend Snippet: ASICs and BK channels are co-expressed in cortical neurons. Detection of the expression of ASICs and BK channels in cortical neurons by double-staining immunofluorescence (original magnification ×400). Nuclei were counterstained with Hoechst33258 ( blue ). ASICs and BK channels were labeled with FITC ( green ) and TRITC ( red ). There is a co-expression between ASIC1, ASIC2, ASIC3, and BK channels

    Techniques Used: Expressing, Double Immunofluorescence Staining, Labeling

    2) Product Images from "Alveolar nonselective channels are ASIC1a/α-ENaC channels and contribute to AFC"

    Article Title: Alveolar nonselective channels are ASIC1a/α-ENaC channels and contribute to AFC

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    doi: 10.1152/ajplung.00379.2016

    AT 2 cells contain ACCN2 . A : PCR analysis showing expression of ACCN isoforms in cDNA prepared from AT 2 cells. Only ACCN2 (ASIC1) has a visible band. B : PCR reamplification showing expression of ACCN2 variant 2 (ASIC1a, lane 3 ) and lack of expression of ACCN2 variant 1 (ASIC1b, lane 2 ). NCBI, National Center for Biotechnology Information.
    Figure Legend Snippet: AT 2 cells contain ACCN2 . A : PCR analysis showing expression of ACCN isoforms in cDNA prepared from AT 2 cells. Only ACCN2 (ASIC1) has a visible band. B : PCR reamplification showing expression of ACCN2 variant 2 (ASIC1a, lane 3 ) and lack of expression of ACCN2 variant 1 (ASIC1b, lane 2 ). NCBI, National Center for Biotechnology Information.

    Techniques Used: Polymerase Chain Reaction, Expressing, Variant Assay

    KO mice have no detectable ASIC1a. PCR analysis shows expression of ACCN2 in cDNA prepared from wild-type lungs and from rat lung as a positive control. The cDNA prepared from ASIC1 KO lung has no detectable ACCN2 (ASIC1).
    Figure Legend Snippet: KO mice have no detectable ASIC1a. PCR analysis shows expression of ACCN2 in cDNA prepared from wild-type lungs and from rat lung as a positive control. The cDNA prepared from ASIC1 KO lung has no detectable ACCN2 (ASIC1).

    Techniques Used: Mouse Assay, Polymerase Chain Reaction, Expressing, Positive Control

    NSC channel frequency strongly depends on ASIC1a and α-ENaC presence. A : Western blot showing ASIC1 expression in L2 cells treated with scrambled shRNA or ASIC1 silencing vectors. Although the blot is cropped, there are no other bands in the blot. Reduction of ASIC1 protein positively correlates to amount of ASIC1a shRNA used. B , left : percentage patches with NSC channels present under ASIC1 or α-ENaC knockdown conditions. Right : percentage patches with HSC channels under ASIC1 or α-ENaC knockdown conditions. Numbers above bars indicate total number of patches.
    Figure Legend Snippet: NSC channel frequency strongly depends on ASIC1a and α-ENaC presence. A : Western blot showing ASIC1 expression in L2 cells treated with scrambled shRNA or ASIC1 silencing vectors. Although the blot is cropped, there are no other bands in the blot. Reduction of ASIC1 protein positively correlates to amount of ASIC1a shRNA used. B , left : percentage patches with NSC channels present under ASIC1 or α-ENaC knockdown conditions. Right : percentage patches with HSC channels under ASIC1 or α-ENaC knockdown conditions. Numbers above bars indicate total number of patches.

    Techniques Used: Western Blot, Expressing, shRNA

    Interaction of ASIC1a and α-ENaC subunits. A , top : L2 cell protein lysate immunoprecipitated (IP) for α-ENaC and immunoblotted (IB) for ASIC1. Bottom : L2 cell protein lysate immunoprecipitated for ASIC1 and immunoblotted for α-ENaC. B : quantification of mammalian two-hybrid assay between ASIC1a and ENaC subunits. Normalized luciferase luminescence is proportional to binding affinity to ASIC1a. Negative control represents random association, and positive control represents maximum affinity. Fold increase for α-ENaC (6.6 ± 0.73) indicates a high affinity for ASIC1a. Data represent a total n of 15 ( n = 3 for each condition); * P
    Figure Legend Snippet: Interaction of ASIC1a and α-ENaC subunits. A , top : L2 cell protein lysate immunoprecipitated (IP) for α-ENaC and immunoblotted (IB) for ASIC1. Bottom : L2 cell protein lysate immunoprecipitated for ASIC1 and immunoblotted for α-ENaC. B : quantification of mammalian two-hybrid assay between ASIC1a and ENaC subunits. Normalized luciferase luminescence is proportional to binding affinity to ASIC1a. Negative control represents random association, and positive control represents maximum affinity. Fold increase for α-ENaC (6.6 ± 0.73) indicates a high affinity for ASIC1a. Data represent a total n of 15 ( n = 3 for each condition); * P

    Techniques Used: Immunoprecipitation, Two Hybrid Assay, Luciferase, Binding Assay, Negative Control, Positive Control

    ASIC1a KO increases lung water content and reduces alveolar fluid clearance. A : lung wet wt-to-dry wt ratios. Higher wet wt-to-dry wt ratio indicates increased lung water content and decreased alveolar fluid clearance. The difference in the two groups is significant. Data represent n = 8 for each treatment group. B : Evans blue dye assay showed that alveolar fluid clearance was significantly reduced in ASIC1a KO mice compared with wild-type mice. Amiloride blocked about half of AFC in wild-type mice, but there is little residual AFC after amiloride in ASIC1 KO mice. Data represent a total n = 3 mice for each treatment group; n.s., not significant. C : bronchalveolar lavage (BAL) fluid protein from wild-type and ASIC1 KO mice. There is no significant difference in BAL protein between the two groups ( n = 4 mice for each treatment group; P = 0.424).
    Figure Legend Snippet: ASIC1a KO increases lung water content and reduces alveolar fluid clearance. A : lung wet wt-to-dry wt ratios. Higher wet wt-to-dry wt ratio indicates increased lung water content and decreased alveolar fluid clearance. The difference in the two groups is significant. Data represent n = 8 for each treatment group. B : Evans blue dye assay showed that alveolar fluid clearance was significantly reduced in ASIC1a KO mice compared with wild-type mice. Amiloride blocked about half of AFC in wild-type mice, but there is little residual AFC after amiloride in ASIC1 KO mice. Data represent a total n = 3 mice for each treatment group; n.s., not significant. C : bronchalveolar lavage (BAL) fluid protein from wild-type and ASIC1 KO mice. There is no significant difference in BAL protein between the two groups ( n = 4 mice for each treatment group; P = 0.424).

    Techniques Used: Mouse Assay

    NSC channels are not observable in single-channel patches on lung slices from ASIC1 KO mice. Single-channel recordings were measured from AT 2 cells in lung slices. A : single-channel currents ( right ) and distribution of current amplitudes ( left ) in a patch on an AT 2 cell from a wild-type lung slice, which has both NSC and HSC channels (sometimes overlapping). B : single-channel currents ( right ) and distribution of current amplitudes ( left ) in a patch on an AT 2 cell from an ASIC1 KO lung slice, which has only HSC channels.
    Figure Legend Snippet: NSC channels are not observable in single-channel patches on lung slices from ASIC1 KO mice. Single-channel recordings were measured from AT 2 cells in lung slices. A : single-channel currents ( right ) and distribution of current amplitudes ( left ) in a patch on an AT 2 cell from a wild-type lung slice, which has both NSC and HSC channels (sometimes overlapping). B : single-channel currents ( right ) and distribution of current amplitudes ( left ) in a patch on an AT 2 cell from an ASIC1 KO lung slice, which has only HSC channels.

    Techniques Used: Mouse Assay

    NSC channels are sensitive to ASIC1-modifying toxins. A : NSC channels were activated by venom of the Texas coral snake ( Micrurus tener tener ). B : psalmotoxin-1 isolated from the venom of the spider, Psalmopoeus cambridgei (Trinidad chevron tarantula), is a potent and selective acid-sensing ion channel 1a (ASIC1a) blocker (IC 50 ). When applied to AT 2 cells in primary culture, it uniformly decreased NSC open probability. Neither toxin had any effect on HSC channels. * P
    Figure Legend Snippet: NSC channels are sensitive to ASIC1-modifying toxins. A : NSC channels were activated by venom of the Texas coral snake ( Micrurus tener tener ). B : psalmotoxin-1 isolated from the venom of the spider, Psalmopoeus cambridgei (Trinidad chevron tarantula), is a potent and selective acid-sensing ion channel 1a (ASIC1a) blocker (IC 50 ). When applied to AT 2 cells in primary culture, it uniformly decreased NSC open probability. Neither toxin had any effect on HSC channels. * P

    Techniques Used: Isolation

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    Alomone Labs anti asic1
    <t>ASIC1</t> and ASIC2a protein expression during OGD-Rep in three types of neurons. Cells were treated with OGD-Rep and prepared for immunofluorescence and western blot analysis. A, C. Representative pictures and summary data showing expressions of ASIC1 and ASIC2a using immunofluorescence. B, D. Representative bands and summary data of ASIC1 and ASIC2a expression in western blotting. Data are the mean ± SD in each group. *P
    Anti Asic1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti asic1/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti asic1 - by Bioz Stars, 2022-08
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    ASIC1 and ASIC2a protein expression during OGD-Rep in three types of neurons. Cells were treated with OGD-Rep and prepared for immunofluorescence and western blot analysis. A, C. Representative pictures and summary data showing expressions of ASIC1 and ASIC2a using immunofluorescence. B, D. Representative bands and summary data of ASIC1 and ASIC2a expression in western blotting. Data are the mean ± SD in each group. *P

    Journal: International Journal of Clinical and Experimental Pathology

    Article Title: Down-regulation of ASICs current and the calcium transients by disrupting PICK1 protects primary cultured mouse cortical neurons from OGD-Rep insults

    doi:

    Figure Lengend Snippet: ASIC1 and ASIC2a protein expression during OGD-Rep in three types of neurons. Cells were treated with OGD-Rep and prepared for immunofluorescence and western blot analysis. A, C. Representative pictures and summary data showing expressions of ASIC1 and ASIC2a using immunofluorescence. B, D. Representative bands and summary data of ASIC1 and ASIC2a expression in western blotting. Data are the mean ± SD in each group. *P

    Article Snippet: Cerebral cortical neurons were collected and fixed with 4% paraformaldehyde in PBS for 30 min. Then permeabilized with PBS/0.3% Triton X-100 for 30 min, and all cells were blocked with 2% goat serum and 1% bovine serum albumin (BSA) in PBS for 1 h, and then incubated with anti-ASIC1 (Alomone labs, Israel) or anti-ASIC2a (Alomone labs, Israel) overnight at 4°C.

    Techniques: Expressing, Immunofluorescence, Western Blot

    ASIC1, involved in the enterodynia of PMS offspring rats, was co‐localization with spinal dorsal horn neurons. (A) PMS significantly reduced the pain threshold of the offspring rats at the age of 6 weeks compared with CON rats ( n = 7 for each group, *** P = 0.0006

    Journal: CNS Neuroscience & Therapeutics

    Article Title: Upregulation of spinal ASIC1 by miR‐485 mediates enterodynia in adult offspring rats with prenatal maternal stress, et al. Upregulation of spinal ASIC1 by miR‐485 mediates enterodynia in adult offspring rats with prenatal maternal stress

    doi: 10.1111/cns.13542

    Figure Lengend Snippet: ASIC1, involved in the enterodynia of PMS offspring rats, was co‐localization with spinal dorsal horn neurons. (A) PMS significantly reduced the pain threshold of the offspring rats at the age of 6 weeks compared with CON rats ( n = 7 for each group, *** P = 0.0006

    Article Snippet: The primary antibodies incubated on the spinal cord included anti‐ASIC1 (1:50, Alomone Labs, Jerusalem, Israel), anti‐NeuN (1:50, Merck Millipore, Darmstadt, Germany), anti‐GFAP (1:100, Cell Signaling Technology, Danvers, MA, USA), and anti‐CD11b (1:50, Bio‐Rad, California, USA).

    Techniques:

    MiR‐485 agomir negatively regulated ASIC1 expression and reversed the synaptic transmission of spinal dorsal horn in PMS offspring rats. (A) The expression of ASIC1 was obviously reduced in spinal dorsal horn after intrathecally injecting miR‐485 agomir for consecutive 7 days ( n = 7 for each group, * P

    Journal: CNS Neuroscience & Therapeutics

    Article Title: Upregulation of spinal ASIC1 by miR‐485 mediates enterodynia in adult offspring rats with prenatal maternal stress, et al. Upregulation of spinal ASIC1 by miR‐485 mediates enterodynia in adult offspring rats with prenatal maternal stress

    doi: 10.1111/cns.13542

    Figure Lengend Snippet: MiR‐485 agomir negatively regulated ASIC1 expression and reversed the synaptic transmission of spinal dorsal horn in PMS offspring rats. (A) The expression of ASIC1 was obviously reduced in spinal dorsal horn after intrathecally injecting miR‐485 agomir for consecutive 7 days ( n = 7 for each group, * P

    Article Snippet: The primary antibodies incubated on the spinal cord included anti‐ASIC1 (1:50, Alomone Labs, Jerusalem, Israel), anti‐NeuN (1:50, Merck Millipore, Darmstadt, Germany), anti‐GFAP (1:100, Cell Signaling Technology, Danvers, MA, USA), and anti‐CD11b (1:50, Bio‐Rad, California, USA).

    Techniques: Expressing, Transmission Assay

    MiR‐485 was reduced in the spinal dorsal horn of PMS offspring rats and co‐expressed with ASIC1 in spinal dorsal horn. (A) PMS significantly reduced miR‐485 expression in T13‐L2 spinal dorsal horn at the age of 6 weeks compared with CON ( n = 4 for each group, ** P

    Journal: CNS Neuroscience & Therapeutics

    Article Title: Upregulation of spinal ASIC1 by miR‐485 mediates enterodynia in adult offspring rats with prenatal maternal stress, et al. Upregulation of spinal ASIC1 by miR‐485 mediates enterodynia in adult offspring rats with prenatal maternal stress

    doi: 10.1111/cns.13542

    Figure Lengend Snippet: MiR‐485 was reduced in the spinal dorsal horn of PMS offspring rats and co‐expressed with ASIC1 in spinal dorsal horn. (A) PMS significantly reduced miR‐485 expression in T13‐L2 spinal dorsal horn at the age of 6 weeks compared with CON ( n = 4 for each group, ** P

    Article Snippet: The primary antibodies incubated on the spinal cord included anti‐ASIC1 (1:50, Alomone Labs, Jerusalem, Israel), anti‐NeuN (1:50, Merck Millipore, Darmstadt, Germany), anti‐GFAP (1:100, Cell Signaling Technology, Danvers, MA, USA), and anti‐CD11b (1:50, Bio‐Rad, California, USA).

    Techniques: Expressing

    Expressions of ASIC subunits in rat cardiomyocytes. ( a ) Expressions of ASIC1, 2a and 3 proteins in rat cultured cardiomyocytes. Individual ASIC subunits peptides were added as negative controls. The blots were cropped from Supplementary Fig. S1a . The representative full-length blot for ASIC2a was shown in Supplementary Fig. S2 . ( b ) Expressions of ASIC1, 2 and 3 transcripts in cultured rat ventricular myocytes. GAPDH transcript was used as control. M: marker; 1, 4: cardiomyocytes; 2: cortex; 3: negative control. ( c ) Double - labeling fluorescence of ASICs ( green ) and nucleus ( blue , marker: Hoechst 33258) in cultured ratcardiomyocytes. NC: pretreatment with immunogenic peptide as negative control. Scale bars: 20 μm. All data were represented from at least three similar independent experiments.

    Journal: Scientific Reports

    Article Title: Multiple H+ sensors mediate the extracellular acidification-induced [Ca2+]i elevation in cultured rat ventricular cardiomyocytes

    doi: 10.1038/srep44951

    Figure Lengend Snippet: Expressions of ASIC subunits in rat cardiomyocytes. ( a ) Expressions of ASIC1, 2a and 3 proteins in rat cultured cardiomyocytes. Individual ASIC subunits peptides were added as negative controls. The blots were cropped from Supplementary Fig. S1a . The representative full-length blot for ASIC2a was shown in Supplementary Fig. S2 . ( b ) Expressions of ASIC1, 2 and 3 transcripts in cultured rat ventricular myocytes. GAPDH transcript was used as control. M: marker; 1, 4: cardiomyocytes; 2: cortex; 3: negative control. ( c ) Double - labeling fluorescence of ASICs ( green ) and nucleus ( blue , marker: Hoechst 33258) in cultured ratcardiomyocytes. NC: pretreatment with immunogenic peptide as negative control. Scale bars: 20 μm. All data were represented from at least three similar independent experiments.

    Article Snippet: After blocking, the proteins were probed with the appropriate primary antibodies against ASIC1, ASIC2a, ASIC3, TRPV1 (Alomone labs, Jerusalem, Israel.ASC-014, ASC-012, ASC-018, ACC-030, all in 1:200 dilution) and OGR1 (Santa Cruz Biotechnology, USA. sc-98437).

    Techniques: Cell Culture, Marker, Negative Control, Labeling, Fluorescence