Journal: Stem Cells and Development
Article Title: Acid-Sensitive Ion Channels Are Expressed in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
Figure Lengend Snippet: mRNA expression of ASIC family in hiPSC differentiated cardiomyocytes. (A) Expression of ASIC1 and ASIC2 transcripts in human iPSC-derived cardiac clusters (IMR-90 line) and control cell lines. Beating hiPSC-derived cardiac clusters were collected on days 30 and 100 of differentiation using END2 protocol. hASIC1 mRNA was detected in undifferentiated hiPSCs and hiPSC-CMs (day 30), but was very low in day 100 hiPSC-CM. hiPSC-derived NPCs and hiPSC-NPC-derived neurons were used as positive controls. Human ASIC2 transcripts were detected only in hiPSC-NPC-derived neurons but not in any stage of hiPSC-CMs. (B) ASIC1a , ASIC2a , and ASIC2b section) in 55 days old hiPSC-CMs derived from iPSC-K3 line. (C, D) Quantitative polymerase chain reaction data confirmed the expression of ASIC1a , ASIC2a , and ASIC3 mRNA in hiPSC-CMs derived from both K3 and IMR-90 iPSC lines, n = 3 and n = 4, respectively. The ** p
Article Snippet: The following primary antibodies were used: guinea pig polyclonal anti-ASIC1 (1:200, Cat. No. AGP-053; Alomone Labs), mouse monoclonal anti-α-actinin (1:800, Cat. No. A-7811, Clone: EA-53; Sigma), and rabbit polyclonal anti-MAP2 (1:200, Cat. No. ab32454; Abcam).
Techniques: Expressing, Derivative Assay, Real-time Polymerase Chain Reaction