asics  (Alomone Labs)


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    Structured Review

    Alomone Labs asics
    Asics, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/asics/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    asics - by Bioz Stars, 2022-12
    93/100 stars

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    Alomone Labs guinea pig anti asic1 antibody
    mRNA expression of ASIC family in hiPSC differentiated cardiomyocytes. (A) Expression of <t>ASIC1</t> and ASIC2 transcripts in human iPSC-derived cardiac clusters (IMR-90 line) and control cell lines. Beating hiPSC-derived cardiac clusters were collected on days 30 and 100 of differentiation using END2 protocol. hASIC1 mRNA was detected in undifferentiated hiPSCs and hiPSC-CMs (day 30), but was very low in day 100 hiPSC-CM. hiPSC-derived NPCs and hiPSC-NPC-derived neurons were used as positive controls. Human ASIC2 transcripts were detected only in hiPSC-NPC-derived neurons but not in any stage of hiPSC-CMs. (B) ASIC1a , ASIC2a , and ASIC2b section) in 55 days old hiPSC-CMs derived from iPSC-K3 line. (C, D) Quantitative polymerase chain reaction data confirmed the expression of ASIC1a , ASIC2a , and ASIC3 mRNA in hiPSC-CMs derived from both K3 and IMR-90 iPSC lines, n = 3 and n = 4, respectively. The ** p
    Guinea Pig Anti Asic1 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/guinea pig anti asic1 antibody/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    guinea pig anti asic1 antibody - by Bioz Stars, 2022-12
    93/100 stars
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    mRNA expression of ASIC family in hiPSC differentiated cardiomyocytes. (A) Expression of ASIC1 and ASIC2 transcripts in human iPSC-derived cardiac clusters (IMR-90 line) and control cell lines. Beating hiPSC-derived cardiac clusters were collected on days 30 and 100 of differentiation using END2 protocol. hASIC1 mRNA was detected in undifferentiated hiPSCs and hiPSC-CMs (day 30), but was very low in day 100 hiPSC-CM. hiPSC-derived NPCs and hiPSC-NPC-derived neurons were used as positive controls. Human ASIC2 transcripts were detected only in hiPSC-NPC-derived neurons but not in any stage of hiPSC-CMs. (B) ASIC1a , ASIC2a , and ASIC2b section) in 55 days old hiPSC-CMs derived from iPSC-K3 line. (C, D) Quantitative polymerase chain reaction data confirmed the expression of ASIC1a , ASIC2a , and ASIC3 mRNA in hiPSC-CMs derived from both K3 and IMR-90 iPSC lines, n = 3 and n = 4, respectively. The ** p

    Journal: Stem Cells and Development

    Article Title: Acid-Sensitive Ion Channels Are Expressed in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

    doi: 10.1089/scd.2018.0234

    Figure Lengend Snippet: mRNA expression of ASIC family in hiPSC differentiated cardiomyocytes. (A) Expression of ASIC1 and ASIC2 transcripts in human iPSC-derived cardiac clusters (IMR-90 line) and control cell lines. Beating hiPSC-derived cardiac clusters were collected on days 30 and 100 of differentiation using END2 protocol. hASIC1 mRNA was detected in undifferentiated hiPSCs and hiPSC-CMs (day 30), but was very low in day 100 hiPSC-CM. hiPSC-derived NPCs and hiPSC-NPC-derived neurons were used as positive controls. Human ASIC2 transcripts were detected only in hiPSC-NPC-derived neurons but not in any stage of hiPSC-CMs. (B) ASIC1a , ASIC2a , and ASIC2b section) in 55 days old hiPSC-CMs derived from iPSC-K3 line. (C, D) Quantitative polymerase chain reaction data confirmed the expression of ASIC1a , ASIC2a , and ASIC3 mRNA in hiPSC-CMs derived from both K3 and IMR-90 iPSC lines, n = 3 and n = 4, respectively. The ** p

    Article Snippet: The following primary antibodies were used: guinea pig polyclonal anti-ASIC1 (1:200, Cat. No. AGP-053; Alomone Labs), mouse monoclonal anti-α-actinin (1:800, Cat. No. A-7811, Clone: EA-53; Sigma), and rabbit polyclonal anti-MAP2 (1:200, Cat. No. ab32454; Abcam).

    Techniques: Expressing, Derivative Assay, Real-time Polymerase Chain Reaction

    Assessment of ASIC1 expression in human iPSC-CMs using immunocytochemistry. Representative immunofluorescence panels show colocalization of ASIC1 ( red ) with the cardiomyocyte marker a-actinin ( green ) in human iPSC-derived cardiomyocytes both at day 20 and at day 62 of directed differentiation. The neuroblastoma SH-SY5Y cells were used as positive control and exhibit more intense ASIC1 signal than CMs. Cells incubated only with fluorescently labeled secondary antibodies did not produce any specific signals. Nuclei were counterstained with DAPI ( blue ). CMs were differentiated from NP0040 iPSC line. Scale bars: 50 μm. Color images are available online.

    Journal: Stem Cells and Development

    Article Title: Acid-Sensitive Ion Channels Are Expressed in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

    doi: 10.1089/scd.2018.0234

    Figure Lengend Snippet: Assessment of ASIC1 expression in human iPSC-CMs using immunocytochemistry. Representative immunofluorescence panels show colocalization of ASIC1 ( red ) with the cardiomyocyte marker a-actinin ( green ) in human iPSC-derived cardiomyocytes both at day 20 and at day 62 of directed differentiation. The neuroblastoma SH-SY5Y cells were used as positive control and exhibit more intense ASIC1 signal than CMs. Cells incubated only with fluorescently labeled secondary antibodies did not produce any specific signals. Nuclei were counterstained with DAPI ( blue ). CMs were differentiated from NP0040 iPSC line. Scale bars: 50 μm. Color images are available online.

    Article Snippet: The following primary antibodies were used: guinea pig polyclonal anti-ASIC1 (1:200, Cat. No. AGP-053; Alomone Labs), mouse monoclonal anti-α-actinin (1:800, Cat. No. A-7811, Clone: EA-53; Sigma), and rabbit polyclonal anti-MAP2 (1:200, Cat. No. ab32454; Abcam).

    Techniques: Expressing, Immunocytochemistry, Immunofluorescence, Marker, Derivative Assay, Positive Control, Incubation, Labeling

    Immunohistochemical detection of ASIC1 in adult rat brain and heart tissue cryoslices. Representative immunofluorescence panels show colocalization of ASIC1 with the neuronal marker microtubule-associated protein 2 (Map2) in the adult rat brain but no ASIC1 expression in the adult rat heart muscle cells, which stained positive for the cardiomyocyte marker sarcomeric α-actinin (Actn2). Tissue specimens incubated only with fluorescently labeled secondary antibodies gave no specific signals. Nuclei were counterstained with Hoechst 33342. Scale bars: 100 μm. Color images are available online.

    Journal: Stem Cells and Development

    Article Title: Acid-Sensitive Ion Channels Are Expressed in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

    doi: 10.1089/scd.2018.0234

    Figure Lengend Snippet: Immunohistochemical detection of ASIC1 in adult rat brain and heart tissue cryoslices. Representative immunofluorescence panels show colocalization of ASIC1 with the neuronal marker microtubule-associated protein 2 (Map2) in the adult rat brain but no ASIC1 expression in the adult rat heart muscle cells, which stained positive for the cardiomyocyte marker sarcomeric α-actinin (Actn2). Tissue specimens incubated only with fluorescently labeled secondary antibodies gave no specific signals. Nuclei were counterstained with Hoechst 33342. Scale bars: 100 μm. Color images are available online.

    Article Snippet: The following primary antibodies were used: guinea pig polyclonal anti-ASIC1 (1:200, Cat. No. AGP-053; Alomone Labs), mouse monoclonal anti-α-actinin (1:800, Cat. No. A-7811, Clone: EA-53; Sigma), and rabbit polyclonal anti-MAP2 (1:200, Cat. No. ab32454; Abcam).

    Techniques: Immunohistochemistry, Immunofluorescence, Marker, Expressing, Staining, Incubation, Labeling