asic2a (Alomone Labs)


Structured Review

Asic2a, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/asic2a/product/Alomone Labs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels"
Article Title: Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels
Journal:
doi: 10.1091/mbc.E05-11-1027

Figure Legend Snippet: Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after
Techniques Used:

Figure Legend Snippet: Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded
Techniques Used: Knock-Out, Mouse Assay
2) Product Images from "Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels"
Article Title: Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels
Journal:
doi: 10.1091/mbc.E05-11-1027

Figure Legend Snippet: Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after
Techniques Used:

Figure Legend Snippet: Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded
Techniques Used: Knock-Out, Mouse Assay
3) Product Images from "Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels"
Article Title: Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels
Journal:
doi: 10.1091/mbc.E05-11-1027

Figure Legend Snippet: Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after
Techniques Used:

Figure Legend Snippet: Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded
Techniques Used: Knock-Out, Mouse Assay
4) Product Images from "Acid-sensing ion channels are expressed in the ventrolateral medulla and contribute to central chemoreception"
Article Title: Acid-sensing ion channels are expressed in the ventrolateral medulla and contribute to central chemoreception
Journal: Scientific Reports
doi: 10.1038/srep38777

Figure Legend Snippet: ASIC1 and ASIC 2a were expressed and co-localized in the VLM neurons of adult rat. (a) and ( b) Representative confocal photomicrographs showed co-localization of ASIC1-ir and ASIC2a-ir (green) with neurofilament-ir (red) in the VLM. ( a) and ( b ) are same scale bar (scale bar = 20 μM). (c) Representative confocal photomicrographs showed co-localization of ASIC1 (green) with ASIC2a (red) in the VLM. (d) Representative confocal photomicrographs showed there are very few of neurons expressed ASIC2a (red) only. ( c) and ( d ) are same scale bar (scale bar = 80 μM).
Techniques Used:

Figure Legend Snippet: The distribution of ASIC1 and ASIC2a in the medulla of neonatal and adult rats. (a) and ( b) ASIC1 positive cells in the VLM and DM of medulla in neonatal rats, respectively; ( a1 ) and ( b1 and b2 ) Representative high power visual field of area in ( a ) and ( b ), respectively. ( c ) Negative control. ( d ) and (e) ASIC1 positive cells in the VLM and DM of medulla in adult rats, respectively; ( d1 ) and ( e1 and e2 ) Representative high power visual field of area in ( d ) and ( e ), respectively. ( f ) Absorption control, and antibody was pre-absorbed by ASIC1. ( g ) Number of ASIC1 positive cells was decreased in adult rats compared to neonatal rats (*** p
Techniques Used: Negative Control

Figure Legend Snippet: Expression of ASIC1a and ASIC2a in the medulla of adult and neonatal SD rat. (a) RNA levels of ASIC1a and ASIC2a were measured by real-time qualitative PCR. RNA levels of ASIC1a and ASIC2a were significantly decreased in adult rats as compared to their neonatal rats. The RNA expression levels of ASIC2a is higher than ASIC1a. RNA level of ASIC1a of neonatal rats was set as 1.0, all others were normalized to the ASIC1a of neonatal rats. * p
Techniques Used: Expressing, Polymerase Chain Reaction, RNA Expression
5) Product Images from "Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels"
Article Title: Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels
Journal:
doi: 10.1091/mbc.E05-11-1027

Figure Legend Snippet: Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after
Techniques Used:

Figure Legend Snippet: Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded
Techniques Used: Knock-Out, Mouse Assay
6) Product Images from "Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels"
Article Title: Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels
Journal:
doi: 10.1091/mbc.E05-11-1027

Figure Legend Snippet: Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after
Techniques Used:

Figure Legend Snippet: Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded
Techniques Used: Knock-Out, Mouse Assay
7) Product Images from "Multiple H+ sensors mediate the extracellular acidification-induced [Ca2+]i elevation in cultured rat ventricular cardiomyocytes"
Article Title: Multiple H+ sensors mediate the extracellular acidification-induced [Ca2+]i elevation in cultured rat ventricular cardiomyocytes
Journal: Scientific Reports
doi: 10.1038/srep44951

Figure Legend Snippet: Expressions of ASIC subunits in rat cardiomyocytes. ( a ) Expressions of ASIC1, 2a and 3 proteins in rat cultured cardiomyocytes. Individual ASIC subunits peptides were added as negative controls. The blots were cropped from Supplementary Fig. S1a . The representative full-length blot for ASIC2a was shown in Supplementary Fig. S2 . ( b ) Expressions of ASIC1, 2 and 3 transcripts in cultured rat ventricular myocytes. GAPDH transcript was used as control. M: marker; 1, 4: cardiomyocytes; 2: cortex; 3: negative control. ( c ) Double - labeling fluorescence of ASICs ( green ) and nucleus ( blue , marker: Hoechst 33258) in cultured ratcardiomyocytes. NC: pretreatment with immunogenic peptide as negative control. Scale bars: 20 μm. All data were represented from at least three similar independent experiments.
Techniques Used: Cell Culture, Marker, Negative Control, Labeling, Fluorescence
8) Product Images from "Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels"
Article Title: Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels
Journal:
doi: 10.1091/mbc.E05-11-1027

Figure Legend Snippet: Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after
Techniques Used:

Figure Legend Snippet: Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded
Techniques Used: Knock-Out, Mouse Assay
9) Product Images from "Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels"
Article Title: Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels
Journal:
doi: 10.1091/mbc.E05-11-1027

Figure Legend Snippet: Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after
Techniques Used:

Figure Legend Snippet: Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded
Techniques Used: Knock-Out, Mouse Assay
10) Product Images from "Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels"
Article Title: Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels
Journal:
doi: 10.1091/mbc.E05-11-1027

Figure Legend Snippet: Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after
Techniques Used:

Figure Legend Snippet: Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded
Techniques Used: Knock-Out, Mouse Assay
11) Product Images from "Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels"
Article Title: Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels
Journal:
doi: 10.1091/mbc.E05-11-1027

Figure Legend Snippet: Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after
Techniques Used:

Figure Legend Snippet: Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded
Techniques Used: Knock-Out, Mouse Assay
12) Product Images from "Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels"
Article Title: Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels
Journal:
doi: 10.1091/mbc.E05-11-1027

Figure Legend Snippet: Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after
Techniques Used:

Figure Legend Snippet: Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded
Techniques Used: Knock-Out, Mouse Assay
13) Product Images from "Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels"
Article Title: Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels
Journal:
doi: 10.1091/mbc.E05-11-1027

Figure Legend Snippet: Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after
Techniques Used:

Figure Legend Snippet: Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded
Techniques Used: Knock-Out, Mouse Assay
14) Product Images from "Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels"
Article Title: Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels
Journal:
doi: 10.1091/mbc.E05-11-1027

Figure Legend Snippet: Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after
Techniques Used:

Figure Legend Snippet: Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded
Techniques Used: Knock-Out, Mouse Assay
15) Product Images from "Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels"
Article Title: Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels
Journal:
doi: 10.1091/mbc.E05-11-1027

Figure Legend Snippet: Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after
Techniques Used:

Figure Legend Snippet: Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded
Techniques Used: Knock-Out, Mouse Assay