asic2a  (Alomone Labs)


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    Alomone Labs asic2a
    Expressions of ASIC subunits in rat cardiomyocytes. ( a ) Expressions of ASIC1, 2a and 3 proteins in rat cultured cardiomyocytes. Individual ASIC subunits peptides were added as negative controls. The blots were cropped from Supplementary Fig. S1a . The representative full-length blot for <t>ASIC2a</t> was shown in Supplementary Fig. S2 . ( b ) Expressions of ASIC1, 2 and 3 transcripts in cultured rat ventricular myocytes. GAPDH transcript was used as control. M: marker; 1, 4: cardiomyocytes; 2: cortex; 3: negative control. ( c ) Double - labeling fluorescence of ASICs ( green ) and nucleus ( blue , marker: Hoechst 33258) in cultured ratcardiomyocytes. NC: pretreatment with immunogenic peptide as negative control. Scale bars: 20 μm. All data were represented from at least three similar independent experiments.
    Asic2a, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/asic2a/product/Alomone Labs
    Average 93 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    asic2a - by Bioz Stars, 2022-12
    93/100 stars

    Images

    1) Product Images from "Multiple H+ sensors mediate the extracellular acidification-induced [Ca2+]i elevation in cultured rat ventricular cardiomyocytes"

    Article Title: Multiple H+ sensors mediate the extracellular acidification-induced [Ca2+]i elevation in cultured rat ventricular cardiomyocytes

    Journal: Scientific Reports

    doi: 10.1038/srep44951

    Expressions of ASIC subunits in rat cardiomyocytes. ( a ) Expressions of ASIC1, 2a and 3 proteins in rat cultured cardiomyocytes. Individual ASIC subunits peptides were added as negative controls. The blots were cropped from Supplementary Fig. S1a . The representative full-length blot for ASIC2a was shown in Supplementary Fig. S2 . ( b ) Expressions of ASIC1, 2 and 3 transcripts in cultured rat ventricular myocytes. GAPDH transcript was used as control. M: marker; 1, 4: cardiomyocytes; 2: cortex; 3: negative control. ( c ) Double - labeling fluorescence of ASICs ( green ) and nucleus ( blue , marker: Hoechst 33258) in cultured ratcardiomyocytes. NC: pretreatment with immunogenic peptide as negative control. Scale bars: 20 μm. All data were represented from at least three similar independent experiments.
    Figure Legend Snippet: Expressions of ASIC subunits in rat cardiomyocytes. ( a ) Expressions of ASIC1, 2a and 3 proteins in rat cultured cardiomyocytes. Individual ASIC subunits peptides were added as negative controls. The blots were cropped from Supplementary Fig. S1a . The representative full-length blot for ASIC2a was shown in Supplementary Fig. S2 . ( b ) Expressions of ASIC1, 2 and 3 transcripts in cultured rat ventricular myocytes. GAPDH transcript was used as control. M: marker; 1, 4: cardiomyocytes; 2: cortex; 3: negative control. ( c ) Double - labeling fluorescence of ASICs ( green ) and nucleus ( blue , marker: Hoechst 33258) in cultured ratcardiomyocytes. NC: pretreatment with immunogenic peptide as negative control. Scale bars: 20 μm. All data were represented from at least three similar independent experiments.

    Techniques Used: Cell Culture, Marker, Negative Control, Labeling, Fluorescence

    2) Product Images from "Acid-sensing ion channels are expressed in the ventrolateral medulla and contribute to central chemoreception"

    Article Title: Acid-sensing ion channels are expressed in the ventrolateral medulla and contribute to central chemoreception

    Journal: Scientific Reports

    doi: 10.1038/srep38777

    ASIC1 and ASIC 2a were expressed and co-localized in the VLM neurons of adult rat. (a) and ( b) Representative confocal photomicrographs showed co-localization of ASIC1-ir and ASIC2a-ir (green) with neurofilament-ir (red) in the VLM. ( a) and ( b ) are same scale bar (scale bar = 20 μM). (c) Representative confocal photomicrographs showed co-localization of ASIC1 (green) with ASIC2a (red) in the VLM. (d) Representative confocal photomicrographs showed there are very few of neurons expressed ASIC2a (red) only. ( c) and ( d ) are same scale bar (scale bar = 80 μM).
    Figure Legend Snippet: ASIC1 and ASIC 2a were expressed and co-localized in the VLM neurons of adult rat. (a) and ( b) Representative confocal photomicrographs showed co-localization of ASIC1-ir and ASIC2a-ir (green) with neurofilament-ir (red) in the VLM. ( a) and ( b ) are same scale bar (scale bar = 20 μM). (c) Representative confocal photomicrographs showed co-localization of ASIC1 (green) with ASIC2a (red) in the VLM. (d) Representative confocal photomicrographs showed there are very few of neurons expressed ASIC2a (red) only. ( c) and ( d ) are same scale bar (scale bar = 80 μM).

    Techniques Used:

    The distribution of ASIC1 and ASIC2a in the medulla of neonatal and adult rats. (a) and ( b) ASIC1 positive cells in the VLM and DM of medulla in neonatal rats, respectively; ( a1 ) and ( b1 and b2 ) Representative high power visual field of area in ( a ) and ( b ), respectively. ( c ) Negative control. ( d ) and (e) ASIC1 positive cells in the VLM and DM of medulla in adult rats, respectively; ( d1 ) and ( e1 and e2 ) Representative high power visual field of area in ( d ) and ( e ), respectively. ( f ) Absorption control, and antibody was pre-absorbed by ASIC1. ( g ) Number of ASIC1 positive cells was decreased in adult rats compared to neonatal rats (*** p
    Figure Legend Snippet: The distribution of ASIC1 and ASIC2a in the medulla of neonatal and adult rats. (a) and ( b) ASIC1 positive cells in the VLM and DM of medulla in neonatal rats, respectively; ( a1 ) and ( b1 and b2 ) Representative high power visual field of area in ( a ) and ( b ), respectively. ( c ) Negative control. ( d ) and (e) ASIC1 positive cells in the VLM and DM of medulla in adult rats, respectively; ( d1 ) and ( e1 and e2 ) Representative high power visual field of area in ( d ) and ( e ), respectively. ( f ) Absorption control, and antibody was pre-absorbed by ASIC1. ( g ) Number of ASIC1 positive cells was decreased in adult rats compared to neonatal rats (*** p

    Techniques Used: Negative Control

    Expression of ASIC1a and ASIC2a in the medulla of adult and neonatal SD rat. (a) RNA levels of ASIC1a and ASIC2a were measured by real-time qualitative PCR. RNA levels of ASIC1a and ASIC2a were significantly decreased in adult rats as compared to their neonatal rats. The RNA expression levels of ASIC2a is higher than ASIC1a. RNA level of ASIC1a of neonatal rats was set as 1.0, all others were normalized to the ASIC1a of neonatal rats. * p
    Figure Legend Snippet: Expression of ASIC1a and ASIC2a in the medulla of adult and neonatal SD rat. (a) RNA levels of ASIC1a and ASIC2a were measured by real-time qualitative PCR. RNA levels of ASIC1a and ASIC2a were significantly decreased in adult rats as compared to their neonatal rats. The RNA expression levels of ASIC2a is higher than ASIC1a. RNA level of ASIC1a of neonatal rats was set as 1.0, all others were normalized to the ASIC1a of neonatal rats. * p

    Techniques Used: Expressing, Polymerase Chain Reaction, RNA Expression

    3) Product Images from "Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels"

    Article Title: Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels

    Journal:

    doi: 10.1091/mbc.E05-11-1027

    Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after
    Figure Legend Snippet: Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after

    Techniques Used:

    Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded
    Figure Legend Snippet: Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded

    Techniques Used: Knock-Out, Mouse Assay

    4) Product Images from "Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels"

    Article Title: Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels

    Journal:

    doi: 10.1091/mbc.E05-11-1027

    Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after
    Figure Legend Snippet: Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after

    Techniques Used:

    Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded
    Figure Legend Snippet: Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded

    Techniques Used: Knock-Out, Mouse Assay

    5) Product Images from "Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels"

    Article Title: Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels

    Journal:

    doi: 10.1091/mbc.E05-11-1027

    Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after
    Figure Legend Snippet: Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after

    Techniques Used:

    Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded
    Figure Legend Snippet: Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded

    Techniques Used: Knock-Out, Mouse Assay

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    • anti asic2a  (Alomone Labs)
    93
    Alomone Labs anti asic2a
    Functional expression of ASIC2 in X. laevis oocyte. ( A ) ASIC2b coexpression attenuates <t>ASIC2a</t> desensitization. Original traces of whole-cell current (holding potential = –70 mV) in oocytes expressing ASIC2a alone or with ASIC2b, or t-ASIC2b, as indicated below the traces. Inward currents were induced by a rapid extracellular acidification from pH 7.4 to pH 4.0 (indicated by the horizontal bar). ( B ) Mean transient-induced (peak) and residual-induced (plateau) currents (corrected by currents recorded in control oocytes) in oocytes expressing ASIC2a alone (black bars) or coexpressing ASIC2b (red) or t-ASIC2b (blue). ( C ) Acid-induced plateau currents (normalized to maximal value achieved at pH 4) at different extracellular pH; colors as above. ( D ) Effect of amiloride: acid-induced (pH 4) plateau currents (normalized to the value measured in the absence of inhibitor) in the presence of 100 μM amiloride. Data are shown as mean ± SEM (each value represents an oocyte, n is shown in italic in each figure). Comparison between groups was performed by variance analysis (1-way ANOVA) followed by post hoc multiple comparison Tukey’s test. P
    Anti Asic2a, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti asic2a/product/Alomone Labs
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    anti asic2a - by Bioz Stars, 2022-12
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Functional expression of ASIC2 in X. laevis oocyte. ( A ) ASIC2b coexpression attenuates ASIC2a desensitization. Original traces of whole-cell current (holding potential = –70 mV) in oocytes expressing ASIC2a alone or with ASIC2b, or t-ASIC2b, as indicated below the traces. Inward currents were induced by a rapid extracellular acidification from pH 7.4 to pH 4.0 (indicated by the horizontal bar). ( B ) Mean transient-induced (peak) and residual-induced (plateau) currents (corrected by currents recorded in control oocytes) in oocytes expressing ASIC2a alone (black bars) or coexpressing ASIC2b (red) or t-ASIC2b (blue). ( C ) Acid-induced plateau currents (normalized to maximal value achieved at pH 4) at different extracellular pH; colors as above. ( D ) Effect of amiloride: acid-induced (pH 4) plateau currents (normalized to the value measured in the absence of inhibitor) in the presence of 100 μM amiloride. Data are shown as mean ± SEM (each value represents an oocyte, n is shown in italic in each figure). Comparison between groups was performed by variance analysis (1-way ANOVA) followed by post hoc multiple comparison Tukey’s test. P

    Journal: JCI Insight

    Article Title: A variant of ASIC2 mediates sodium retention in nephrotic syndrome

    doi: 10.1172/jci.insight.148588

    Figure Lengend Snippet: Functional expression of ASIC2 in X. laevis oocyte. ( A ) ASIC2b coexpression attenuates ASIC2a desensitization. Original traces of whole-cell current (holding potential = –70 mV) in oocytes expressing ASIC2a alone or with ASIC2b, or t-ASIC2b, as indicated below the traces. Inward currents were induced by a rapid extracellular acidification from pH 7.4 to pH 4.0 (indicated by the horizontal bar). ( B ) Mean transient-induced (peak) and residual-induced (plateau) currents (corrected by currents recorded in control oocytes) in oocytes expressing ASIC2a alone (black bars) or coexpressing ASIC2b (red) or t-ASIC2b (blue). ( C ) Acid-induced plateau currents (normalized to maximal value achieved at pH 4) at different extracellular pH; colors as above. ( D ) Effect of amiloride: acid-induced (pH 4) plateau currents (normalized to the value measured in the absence of inhibitor) in the presence of 100 μM amiloride. Data are shown as mean ± SEM (each value represents an oocyte, n is shown in italic in each figure). Comparison between groups was performed by variance analysis (1-way ANOVA) followed by post hoc multiple comparison Tukey’s test. P

    Article Snippet: An anti-ASIC2a–specific antibody (anti-ASIC2a, ASC-012, Alomone) revealed a single band approximately 80 kDa in kidney from CC-PAN rats.

    Techniques: Functional Assay, Expressing

    Renal expression of ASIC2. ( A and B ) Western blot analysis of ASIC2 expression in kidney of WT and ASIC2b –/– CC-PAN rats and in CCDs of CC-Control and CC-PAN rats using a specific ASIC2a antibody ( A ) or a pan-ASIC2 antibody ( B ). Left, representative blot; right, densitometric analysis. Each value represents a rat. ( C ) Immunolabeling of isolated CCD from WT and ASIC2b –/– CC-Control and CC-PAN rats with a pan-ASIC2 antibody. Scale bar: 10 μm. ( D ) Immunolabelling of isolated CCD from PAN WT rat with a pan-ASIC2 antibody (green) and an anti-AE1 antibody (red). Comparison between groups was performed by 2-tailed unpaired t test. P

    Journal: JCI Insight

    Article Title: A variant of ASIC2 mediates sodium retention in nephrotic syndrome

    doi: 10.1172/jci.insight.148588

    Figure Lengend Snippet: Renal expression of ASIC2. ( A and B ) Western blot analysis of ASIC2 expression in kidney of WT and ASIC2b –/– CC-PAN rats and in CCDs of CC-Control and CC-PAN rats using a specific ASIC2a antibody ( A ) or a pan-ASIC2 antibody ( B ). Left, representative blot; right, densitometric analysis. Each value represents a rat. ( C ) Immunolabeling of isolated CCD from WT and ASIC2b –/– CC-Control and CC-PAN rats with a pan-ASIC2 antibody. Scale bar: 10 μm. ( D ) Immunolabelling of isolated CCD from PAN WT rat with a pan-ASIC2 antibody (green) and an anti-AE1 antibody (red). Comparison between groups was performed by 2-tailed unpaired t test. P

    Article Snippet: An anti-ASIC2a–specific antibody (anti-ASIC2a, ASC-012, Alomone) revealed a single band approximately 80 kDa in kidney from CC-PAN rats.

    Techniques: Expressing, Western Blot, Immunolabeling, Isolation

    Glycosylation of t-ASIC2b. ( A ) Western blot analysis of ASIC2 expression in X . laevis oocytes injected with ASIC2b or t-ASIC2b cRNA or water and in protein extracts from CC-PAN rat kidneys. Samples were treated or not with N-glycosidase F (PNGase). ( B ) Original traces of whole-cell current (holding potential = –70 mV) in oocytes expressing ASIC2a with t-ASIC2b or a nonglycosylable form of truncated ASIC2b (ng-t-ASIC2b). Inward currents were induced by a rapid extracellular acidification from pH 7.4 to pH 4.0 (indicated by the horizontal bar). ( C ) Acid-induced (pH 4) plateau currents in the absence or presence of 100 μM amiloride. Data are shown as mean ± SEM (each value represents an oocyte, n is shown in italic in the figure). Comparison between groups was performed by 2-tailed unpaired t test. P

    Journal: JCI Insight

    Article Title: A variant of ASIC2 mediates sodium retention in nephrotic syndrome

    doi: 10.1172/jci.insight.148588

    Figure Lengend Snippet: Glycosylation of t-ASIC2b. ( A ) Western blot analysis of ASIC2 expression in X . laevis oocytes injected with ASIC2b or t-ASIC2b cRNA or water and in protein extracts from CC-PAN rat kidneys. Samples were treated or not with N-glycosidase F (PNGase). ( B ) Original traces of whole-cell current (holding potential = –70 mV) in oocytes expressing ASIC2a with t-ASIC2b or a nonglycosylable form of truncated ASIC2b (ng-t-ASIC2b). Inward currents were induced by a rapid extracellular acidification from pH 7.4 to pH 4.0 (indicated by the horizontal bar). ( C ) Acid-induced (pH 4) plateau currents in the absence or presence of 100 μM amiloride. Data are shown as mean ± SEM (each value represents an oocyte, n is shown in italic in the figure). Comparison between groups was performed by 2-tailed unpaired t test. P

    Article Snippet: An anti-ASIC2a–specific antibody (anti-ASIC2a, ASC-012, Alomone) revealed a single band approximately 80 kDa in kidney from CC-PAN rats.

    Techniques: Western Blot, Expressing, Injection