asic2a  (Alomone Labs)


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    Name:
    Anti ASIC2a Antibody
    Description:
    Anti ASIC2a Antibody ASC 012 is a highly specific antibody directed against an epitope of the human protein The antibody can be used in western blot immunoprecipitation immunohistochemistry and immunocytochemistry applications It has been designed to recognize ASIC2a from human rat and mouse samples The antibody is specific for ASIC2a and will not recognize the ASIC2b variant
    Catalog Number:
    ASC-012
    Price:
    397.0
    Category:
    Primary Antibody
    Applications:
    Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
    Purity:
    Affinity purified on immobilized antigen.
    Immunogen:
    Synthetic peptide
    Size:
    25 mcl
    Antibody Type:
    Polyclonal Primary Antibodies
    Format:
    Lyophilized Powder
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
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    Structured Review

    Alomone Labs asic2a
    Anti ASIC2a Antibody
    Anti ASIC2a Antibody ASC 012 is a highly specific antibody directed against an epitope of the human protein The antibody can be used in western blot immunoprecipitation immunohistochemistry and immunocytochemistry applications It has been designed to recognize ASIC2a from human rat and mouse samples The antibody is specific for ASIC2a and will not recognize the ASIC2b variant
    https://www.bioz.com/result/asic2a/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    asic2a - by Bioz Stars, 2021-09
    94/100 stars

    Images

    1) Product Images from "Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels"

    Article Title: Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels

    Journal:

    doi: 10.1091/mbc.E05-11-1027

    Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after
    Figure Legend Snippet: Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after

    Techniques Used:

    Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded
    Figure Legend Snippet: Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded

    Techniques Used: Knock-Out, Mouse Assay

    2) Product Images from "Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels"

    Article Title: Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels

    Journal:

    doi: 10.1091/mbc.E05-11-1027

    Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after
    Figure Legend Snippet: Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after

    Techniques Used:

    Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded
    Figure Legend Snippet: Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded

    Techniques Used: Knock-Out, Mouse Assay

    3) Product Images from "Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels"

    Article Title: Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels

    Journal:

    doi: 10.1091/mbc.E05-11-1027

    Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after
    Figure Legend Snippet: Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after

    Techniques Used:

    Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded
    Figure Legend Snippet: Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded

    Techniques Used: Knock-Out, Mouse Assay

    4) Product Images from "Acid-sensing ion channels are expressed in the ventrolateral medulla and contribute to central chemoreception"

    Article Title: Acid-sensing ion channels are expressed in the ventrolateral medulla and contribute to central chemoreception

    Journal: Scientific Reports

    doi: 10.1038/srep38777

    ASIC1 and ASIC 2a were expressed and co-localized in the VLM neurons of adult rat. (a) and ( b) Representative confocal photomicrographs showed co-localization of ASIC1-ir and ASIC2a-ir (green) with neurofilament-ir (red) in the VLM. ( a) and ( b ) are same scale bar (scale bar = 20 μM). (c) Representative confocal photomicrographs showed co-localization of ASIC1 (green) with ASIC2a (red) in the VLM. (d) Representative confocal photomicrographs showed there are very few of neurons expressed ASIC2a (red) only. ( c) and ( d ) are same scale bar (scale bar = 80 μM).
    Figure Legend Snippet: ASIC1 and ASIC 2a were expressed and co-localized in the VLM neurons of adult rat. (a) and ( b) Representative confocal photomicrographs showed co-localization of ASIC1-ir and ASIC2a-ir (green) with neurofilament-ir (red) in the VLM. ( a) and ( b ) are same scale bar (scale bar = 20 μM). (c) Representative confocal photomicrographs showed co-localization of ASIC1 (green) with ASIC2a (red) in the VLM. (d) Representative confocal photomicrographs showed there are very few of neurons expressed ASIC2a (red) only. ( c) and ( d ) are same scale bar (scale bar = 80 μM).

    Techniques Used:

    The distribution of ASIC1 and ASIC2a in the medulla of neonatal and adult rats. (a) and ( b) ASIC1 positive cells in the VLM and DM of medulla in neonatal rats, respectively; ( a1 ) and ( b1 and b2 ) Representative high power visual field of area in ( a ) and ( b ), respectively. ( c ) Negative control. ( d ) and (e) ASIC1 positive cells in the VLM and DM of medulla in adult rats, respectively; ( d1 ) and ( e1 and e2 ) Representative high power visual field of area in ( d ) and ( e ), respectively. ( f ) Absorption control, and antibody was pre-absorbed by ASIC1. ( g ) Number of ASIC1 positive cells was decreased in adult rats compared to neonatal rats (*** p
    Figure Legend Snippet: The distribution of ASIC1 and ASIC2a in the medulla of neonatal and adult rats. (a) and ( b) ASIC1 positive cells in the VLM and DM of medulla in neonatal rats, respectively; ( a1 ) and ( b1 and b2 ) Representative high power visual field of area in ( a ) and ( b ), respectively. ( c ) Negative control. ( d ) and (e) ASIC1 positive cells in the VLM and DM of medulla in adult rats, respectively; ( d1 ) and ( e1 and e2 ) Representative high power visual field of area in ( d ) and ( e ), respectively. ( f ) Absorption control, and antibody was pre-absorbed by ASIC1. ( g ) Number of ASIC1 positive cells was decreased in adult rats compared to neonatal rats (*** p

    Techniques Used: Negative Control

    Expression of ASIC1a and ASIC2a in the medulla of adult and neonatal SD rat. (a) RNA levels of ASIC1a and ASIC2a were measured by real-time qualitative PCR. RNA levels of ASIC1a and ASIC2a were significantly decreased in adult rats as compared to their neonatal rats. The RNA expression levels of ASIC2a is higher than ASIC1a. RNA level of ASIC1a of neonatal rats was set as 1.0, all others were normalized to the ASIC1a of neonatal rats. * p
    Figure Legend Snippet: Expression of ASIC1a and ASIC2a in the medulla of adult and neonatal SD rat. (a) RNA levels of ASIC1a and ASIC2a were measured by real-time qualitative PCR. RNA levels of ASIC1a and ASIC2a were significantly decreased in adult rats as compared to their neonatal rats. The RNA expression levels of ASIC2a is higher than ASIC1a. RNA level of ASIC1a of neonatal rats was set as 1.0, all others were normalized to the ASIC1a of neonatal rats. * p

    Techniques Used: Expressing, Polymerase Chain Reaction, RNA Expression

    5) Product Images from "Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels"

    Article Title: Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels

    Journal:

    doi: 10.1091/mbc.E05-11-1027

    Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after
    Figure Legend Snippet: Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after

    Techniques Used:

    Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded
    Figure Legend Snippet: Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded

    Techniques Used: Knock-Out, Mouse Assay

    6) Product Images from "Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels"

    Article Title: Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels

    Journal:

    doi: 10.1091/mbc.E05-11-1027

    Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after
    Figure Legend Snippet: Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after

    Techniques Used:

    Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded
    Figure Legend Snippet: Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded

    Techniques Used: Knock-Out, Mouse Assay

    7) Product Images from "Multiple H+ sensors mediate the extracellular acidification-induced [Ca2+]i elevation in cultured rat ventricular cardiomyocytes"

    Article Title: Multiple H+ sensors mediate the extracellular acidification-induced [Ca2+]i elevation in cultured rat ventricular cardiomyocytes

    Journal: Scientific Reports

    doi: 10.1038/srep44951

    Expressions of ASIC subunits in rat cardiomyocytes. ( a ) Expressions of ASIC1, 2a and 3 proteins in rat cultured cardiomyocytes. Individual ASIC subunits peptides were added as negative controls. The blots were cropped from Supplementary Fig. S1a . The representative full-length blot for ASIC2a was shown in Supplementary Fig. S2 . ( b ) Expressions of ASIC1, 2 and 3 transcripts in cultured rat ventricular myocytes. GAPDH transcript was used as control. M: marker; 1, 4: cardiomyocytes; 2: cortex; 3: negative control. ( c ) Double - labeling fluorescence of ASICs ( green ) and nucleus ( blue , marker: Hoechst 33258) in cultured ratcardiomyocytes. NC: pretreatment with immunogenic peptide as negative control. Scale bars: 20 μm. All data were represented from at least three similar independent experiments.
    Figure Legend Snippet: Expressions of ASIC subunits in rat cardiomyocytes. ( a ) Expressions of ASIC1, 2a and 3 proteins in rat cultured cardiomyocytes. Individual ASIC subunits peptides were added as negative controls. The blots were cropped from Supplementary Fig. S1a . The representative full-length blot for ASIC2a was shown in Supplementary Fig. S2 . ( b ) Expressions of ASIC1, 2 and 3 transcripts in cultured rat ventricular myocytes. GAPDH transcript was used as control. M: marker; 1, 4: cardiomyocytes; 2: cortex; 3: negative control. ( c ) Double - labeling fluorescence of ASICs ( green ) and nucleus ( blue , marker: Hoechst 33258) in cultured ratcardiomyocytes. NC: pretreatment with immunogenic peptide as negative control. Scale bars: 20 μm. All data were represented from at least three similar independent experiments.

    Techniques Used: Cell Culture, Marker, Negative Control, Labeling, Fluorescence

    8) Product Images from "Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels"

    Article Title: Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels

    Journal:

    doi: 10.1091/mbc.E05-11-1027

    Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after
    Figure Legend Snippet: Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after

    Techniques Used:

    Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded
    Figure Legend Snippet: Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded

    Techniques Used: Knock-Out, Mouse Assay

    9) Product Images from "Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels"

    Article Title: Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels

    Journal:

    doi: 10.1091/mbc.E05-11-1027

    Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after
    Figure Legend Snippet: Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after

    Techniques Used:

    Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded
    Figure Legend Snippet: Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded

    Techniques Used: Knock-Out, Mouse Assay

    10) Product Images from "Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels"

    Article Title: Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels

    Journal:

    doi: 10.1091/mbc.E05-11-1027

    Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after
    Figure Legend Snippet: Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after

    Techniques Used:

    Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded
    Figure Legend Snippet: Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded

    Techniques Used: Knock-Out, Mouse Assay

    11) Product Images from "Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels"

    Article Title: Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels

    Journal:

    doi: 10.1091/mbc.E05-11-1027

    Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after
    Figure Legend Snippet: Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after

    Techniques Used:

    Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded
    Figure Legend Snippet: Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded

    Techniques Used: Knock-Out, Mouse Assay

    12) Product Images from "Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels"

    Article Title: Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels

    Journal:

    doi: 10.1091/mbc.E05-11-1027

    Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after
    Figure Legend Snippet: Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after

    Techniques Used:

    Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded
    Figure Legend Snippet: Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded

    Techniques Used: Knock-Out, Mouse Assay

    13) Product Images from "Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels"

    Article Title: Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels

    Journal:

    doi: 10.1091/mbc.E05-11-1027

    Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after
    Figure Legend Snippet: Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after

    Techniques Used:

    Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded
    Figure Legend Snippet: Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded

    Techniques Used: Knock-Out, Mouse Assay

    14) Product Images from "Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels"

    Article Title: Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels

    Journal:

    doi: 10.1091/mbc.E05-11-1027

    Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after
    Figure Legend Snippet: Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after

    Techniques Used:

    Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded
    Figure Legend Snippet: Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded

    Techniques Used: Knock-Out, Mouse Assay

    15) Product Images from "Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels"

    Article Title: Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels

    Journal:

    doi: 10.1091/mbc.E05-11-1027

    Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after
    Figure Legend Snippet: Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after

    Techniques Used:

    Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded
    Figure Legend Snippet: Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded

    Techniques Used: Knock-Out, Mouse Assay

    Related Articles

    Incubation:

    Article Title: Down-regulation of ASICs current and the calcium transients by disrupting PICK1 protects primary cultured mouse cortical neurons from OGD-Rep insults
    Article Snippet: .. Cerebral cortical neurons were collected and fixed with 4% paraformaldehyde in PBS for 30 min. Then permeabilized with PBS/0.3% Triton X-100 for 30 min, and all cells were blocked with 2% goat serum and 1% bovine serum albumin (BSA) in PBS for 1 h, and then incubated with anti-ASIC1 (Alomone labs, Israel) or anti-ASIC2a (Alomone labs, Israel) overnight at 4°C. ..

    Article Title: A new variant of ASIC2 mediates sodium retention in nephrotic syndrome
    Article Snippet: .. After blocking, blots were successively incubated with specific anti-ASIC2a antibody (Alomone labs, ASC-012, 1/1000) or pan-ASIC2 antibody (Abcam, ab77384, 1/700) and anti-rabbit IgG antibody coupled to horseradish peroxidase (Promega France, W401B, 1/2500) and revealed with the Western Lightning chemiluminescence reagent Plus (PerkinElmer Life Sciences). ..

    Article Title: Acid-sensing ion channels are expressed in the ventrolateral medulla and contribute to central chemoreception
    Article Snippet: .. The slides were incubated with first antibody of ASIC1 or ASIC2a (Alomone Lab, Israel, rabbit anti rat IgG, 1:100) diluted in 1% bovine serum albumin buffer (1% BSA) and the controls were in 1% BSA or in the antibody which was absorbed by relevant peptides before use for 24 h in 4 °C. ..

    Blocking Assay:

    Article Title: Multiple H+ sensors mediate the extracellular acidification-induced [Ca2+]i elevation in cultured rat ventricular cardiomyocytes
    Article Snippet: .. After blocking, the proteins were probed with the appropriate primary antibodies against ASIC1, ASIC2a, ASIC3, TRPV1 (Alomone labs, Jerusalem, Israel.ASC-014, ASC-012, ASC-018, ACC-030, all in 1:200 dilution) and OGR1 (Santa Cruz Biotechnology, USA. sc-98437). ..

    Article Title: A new variant of ASIC2 mediates sodium retention in nephrotic syndrome
    Article Snippet: .. After blocking, blots were successively incubated with specific anti-ASIC2a antibody (Alomone labs, ASC-012, 1/1000) or pan-ASIC2 antibody (Abcam, ab77384, 1/700) and anti-rabbit IgG antibody coupled to horseradish peroxidase (Promega France, W401B, 1/2500) and revealed with the Western Lightning chemiluminescence reagent Plus (PerkinElmer Life Sciences). ..

    Western Blot:

    Article Title: A new variant of ASIC2 mediates sodium retention in nephrotic syndrome
    Article Snippet: .. After blocking, blots were successively incubated with specific anti-ASIC2a antibody (Alomone labs, ASC-012, 1/1000) or pan-ASIC2 antibody (Abcam, ab77384, 1/700) and anti-rabbit IgG antibody coupled to horseradish peroxidase (Promega France, W401B, 1/2500) and revealed with the Western Lightning chemiluminescence reagent Plus (PerkinElmer Life Sciences). ..

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    Alomone Labs asic2a
    Parkin suppresses PICK1-mediated potentiation of <t>ASIC2a</t> currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after
    Asic2a, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/asic2a/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
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    Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after

    Journal:

    Article Title: Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels

    doi: 10.1091/mbc.E05-11-1027

    Figure Lengend Snippet: Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after

    Article Snippet: The antibodies used were: mouse monoclonal Flag and synaptophysin (Sigma-Aldrich), PSD95, NR1, CASK and synuclein (BD Bioscience, San Jose, CA), ubiquitin (Covance, Madison, WI), 9E10 myc, C2 actin, and PRK8 parkin (Santa Cruz Biotechnology, Santa Cruz, CA); rabbit polyclonal MALS-1/Veli-1 (Zymed, San Francisco, CA), ASIC2a (Alomone Laboratories, Jerusalem, Israel), PICK1 (kind gift from Dr. Richard Huganir, Johns Hopkins, Baltimore, MD), PICK1 (Affinity BioReagents, Golden, CO), and parkin (Cell Signaling, Beverly, MA); goat polyclonal N-18 PICK1 (Santa Cruz).

    Techniques:

    Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded

    Journal:

    Article Title: Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels

    doi: 10.1091/mbc.E05-11-1027

    Figure Lengend Snippet: Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded

    Article Snippet: The antibodies used were: mouse monoclonal Flag and synaptophysin (Sigma-Aldrich), PSD95, NR1, CASK and synuclein (BD Bioscience, San Jose, CA), ubiquitin (Covance, Madison, WI), 9E10 myc, C2 actin, and PRK8 parkin (Santa Cruz Biotechnology, Santa Cruz, CA); rabbit polyclonal MALS-1/Veli-1 (Zymed, San Francisco, CA), ASIC2a (Alomone Laboratories, Jerusalem, Israel), PICK1 (kind gift from Dr. Richard Huganir, Johns Hopkins, Baltimore, MD), PICK1 (Affinity BioReagents, Golden, CO), and parkin (Cell Signaling, Beverly, MA); goat polyclonal N-18 PICK1 (Santa Cruz).

    Techniques: Knock-Out, Mouse Assay

    ASIC1 and ASIC 2a were expressed and co-localized in the VLM neurons of adult rat. (a) and ( b) Representative confocal photomicrographs showed co-localization of ASIC1-ir and ASIC2a-ir (green) with neurofilament-ir (red) in the VLM. ( a) and ( b ) are same scale bar (scale bar = 20 μM). (c) Representative confocal photomicrographs showed co-localization of ASIC1 (green) with ASIC2a (red) in the VLM. (d) Representative confocal photomicrographs showed there are very few of neurons expressed ASIC2a (red) only. ( c) and ( d ) are same scale bar (scale bar = 80 μM).

    Journal: Scientific Reports

    Article Title: Acid-sensing ion channels are expressed in the ventrolateral medulla and contribute to central chemoreception

    doi: 10.1038/srep38777

    Figure Lengend Snippet: ASIC1 and ASIC 2a were expressed and co-localized in the VLM neurons of adult rat. (a) and ( b) Representative confocal photomicrographs showed co-localization of ASIC1-ir and ASIC2a-ir (green) with neurofilament-ir (red) in the VLM. ( a) and ( b ) are same scale bar (scale bar = 20 μM). (c) Representative confocal photomicrographs showed co-localization of ASIC1 (green) with ASIC2a (red) in the VLM. (d) Representative confocal photomicrographs showed there are very few of neurons expressed ASIC2a (red) only. ( c) and ( d ) are same scale bar (scale bar = 80 μM).

    Article Snippet: The slides were incubated with first antibody of ASIC1 or ASIC2a (Alomone Lab, Israel, rabbit anti rat IgG, 1:100) diluted in 1% bovine serum albumin buffer (1% BSA) and the controls were in 1% BSA or in the antibody which was absorbed by relevant peptides before use for 24 h in 4 °C.

    Techniques:

    The distribution of ASIC1 and ASIC2a in the medulla of neonatal and adult rats. (a) and ( b) ASIC1 positive cells in the VLM and DM of medulla in neonatal rats, respectively; ( a1 ) and ( b1 and b2 ) Representative high power visual field of area in ( a ) and ( b ), respectively. ( c ) Negative control. ( d ) and (e) ASIC1 positive cells in the VLM and DM of medulla in adult rats, respectively; ( d1 ) and ( e1 and e2 ) Representative high power visual field of area in ( d ) and ( e ), respectively. ( f ) Absorption control, and antibody was pre-absorbed by ASIC1. ( g ) Number of ASIC1 positive cells was decreased in adult rats compared to neonatal rats (*** p

    Journal: Scientific Reports

    Article Title: Acid-sensing ion channels are expressed in the ventrolateral medulla and contribute to central chemoreception

    doi: 10.1038/srep38777

    Figure Lengend Snippet: The distribution of ASIC1 and ASIC2a in the medulla of neonatal and adult rats. (a) and ( b) ASIC1 positive cells in the VLM and DM of medulla in neonatal rats, respectively; ( a1 ) and ( b1 and b2 ) Representative high power visual field of area in ( a ) and ( b ), respectively. ( c ) Negative control. ( d ) and (e) ASIC1 positive cells in the VLM and DM of medulla in adult rats, respectively; ( d1 ) and ( e1 and e2 ) Representative high power visual field of area in ( d ) and ( e ), respectively. ( f ) Absorption control, and antibody was pre-absorbed by ASIC1. ( g ) Number of ASIC1 positive cells was decreased in adult rats compared to neonatal rats (*** p

    Article Snippet: The slides were incubated with first antibody of ASIC1 or ASIC2a (Alomone Lab, Israel, rabbit anti rat IgG, 1:100) diluted in 1% bovine serum albumin buffer (1% BSA) and the controls were in 1% BSA or in the antibody which was absorbed by relevant peptides before use for 24 h in 4 °C.

    Techniques: Negative Control

    Expression of ASIC1a and ASIC2a in the medulla of adult and neonatal SD rat. (a) RNA levels of ASIC1a and ASIC2a were measured by real-time qualitative PCR. RNA levels of ASIC1a and ASIC2a were significantly decreased in adult rats as compared to their neonatal rats. The RNA expression levels of ASIC2a is higher than ASIC1a. RNA level of ASIC1a of neonatal rats was set as 1.0, all others were normalized to the ASIC1a of neonatal rats. * p

    Journal: Scientific Reports

    Article Title: Acid-sensing ion channels are expressed in the ventrolateral medulla and contribute to central chemoreception

    doi: 10.1038/srep38777

    Figure Lengend Snippet: Expression of ASIC1a and ASIC2a in the medulla of adult and neonatal SD rat. (a) RNA levels of ASIC1a and ASIC2a were measured by real-time qualitative PCR. RNA levels of ASIC1a and ASIC2a were significantly decreased in adult rats as compared to their neonatal rats. The RNA expression levels of ASIC2a is higher than ASIC1a. RNA level of ASIC1a of neonatal rats was set as 1.0, all others were normalized to the ASIC1a of neonatal rats. * p

    Article Snippet: The slides were incubated with first antibody of ASIC1 or ASIC2a (Alomone Lab, Israel, rabbit anti rat IgG, 1:100) diluted in 1% bovine serum albumin buffer (1% BSA) and the controls were in 1% BSA or in the antibody which was absorbed by relevant peptides before use for 24 h in 4 °C.

    Techniques: Expressing, Polymerase Chain Reaction, RNA Expression