red fluorescence conjugated nav1 6 primary antibody  (Alomone Labs)


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    Name:
    Anti SCN1A Nav1 1 ATTO Fluor 594 Antibody
    Description:
    Anti SCN1A NaV1 1 Antibody ASC 001 is a highly specific antibody directed against an epitope of the rat protein The antibody can be used in western blot immunoprecipitation immunohistochemistry and immunocytochemistry applications It has been designed to recognize NaV1 1 from rat human and mouse samples nAnti SCN1A NaV1 1 ATTO Fluor 594 Antibody ASC 001 AR is directly labeled with an ATTO 594 fluorescent dye ATTO dyes are characterized by strong absorption high extinction coefficient high fluorescence quantum yield and high photo stability The ATTO 594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa 594 Anti SCN1A NaV1 1 ATTO Fluor 594 Antibody is specially suited to experiments requiring simultaneous labeling of different markers
    Catalog Number:
    ASC-001-AR
    Price:
    686.0
    Category:
    Primary Antibody
    Applications:
    Immunofluorescence, Immunohistochemistry
    Purity:
    Affinity purified on immobilized antigen.
    Immunogen:
    Synthetic peptide
    Size:
    50 mcl
    Antibody Type:
    Polyclonal ATTO 594 (Red) Conjugated Primary Antibody
    Format:
    Lyophilized Powder
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
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    Structured Review

    Alomone Labs red fluorescence conjugated nav1 6 primary antibody
    Anti SCN1A Nav1 1 ATTO Fluor 594 Antibody
    Anti SCN1A NaV1 1 Antibody ASC 001 is a highly specific antibody directed against an epitope of the rat protein The antibody can be used in western blot immunoprecipitation immunohistochemistry and immunocytochemistry applications It has been designed to recognize NaV1 1 from rat human and mouse samples nAnti SCN1A NaV1 1 ATTO Fluor 594 Antibody ASC 001 AR is directly labeled with an ATTO 594 fluorescent dye ATTO dyes are characterized by strong absorption high extinction coefficient high fluorescence quantum yield and high photo stability The ATTO 594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa 594 Anti SCN1A NaV1 1 ATTO Fluor 594 Antibody is specially suited to experiments requiring simultaneous labeling of different markers
    https://www.bioz.com/result/red fluorescence conjugated nav1 6 primary antibody/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    red fluorescence conjugated nav1 6 primary antibody - by Bioz Stars, 2021-09
    92/100 stars

    Images

    1) Product Images from "Circuit-Specific Early Impairment of Proprioceptive Sensory Neurons in the SOD1G93A Mouse Model for ALS"

    Article Title: Circuit-Specific Early Impairment of Proprioceptive Sensory Neurons in the SOD1G93A Mouse Model for ALS

    Journal: bioRxiv

    doi: 10.1101/669788

    Rescue of Nav 1.6-type sodium currents in mSOD1 Mes V neurons using dynamic-clamp. a. Schematic shows the dynamic-clamp setup used to introduce conductance-based models of Nav1.6-type Na + currents into mSOD1 Mes V neurons in real-time during whole-cell patch-clamp recording; I dyn is the computer-generated model Na + current in combination with a step depolarization to drive the patched Mes V neuron; V m is the measured membrane voltage. b. Representative traces showing the membrane voltage in a bursting mSOD1 Mes V neuron with control/default behavior ( left red ), followed by addition of Nav1.6-type currents ( middle black ), that restores WT-like rhythmic bursting; subsequent removal of added currents returns default mSOD1 behavior ( right red ) in this neuron; Lower green trace shows I dyn . c. Time series plots of IEIs (log scale) for the three different conditions in ( b ); each dot represents an interval between two consecutive spikes (see detailed results). d. Autocorrelation function of the membrane voltage for default mSOD1 ( left ) and with addition of Nav1.6 currents (middle); the height of the 2 nd autocorrelation peak highlights rhythmicity (arrowhead); Right: measured 2 nd peak values for 4 mSOD1 cells are shown, with dashed horizontal line showing average WT values and shaded grey region indicates ±SD. e. Treatment effects on burst characteristics including burst duration (BD), inter-burst-intervals (IBI) and inter-spike-intervals within bursts (ISI) shown for various mSOD1 cells tested under the different conditions as in ( b ); dashed lines indicate average WT values with grey regions marking the ± s.d.
    Figure Legend Snippet: Rescue of Nav 1.6-type sodium currents in mSOD1 Mes V neurons using dynamic-clamp. a. Schematic shows the dynamic-clamp setup used to introduce conductance-based models of Nav1.6-type Na + currents into mSOD1 Mes V neurons in real-time during whole-cell patch-clamp recording; I dyn is the computer-generated model Na + current in combination with a step depolarization to drive the patched Mes V neuron; V m is the measured membrane voltage. b. Representative traces showing the membrane voltage in a bursting mSOD1 Mes V neuron with control/default behavior ( left red ), followed by addition of Nav1.6-type currents ( middle black ), that restores WT-like rhythmic bursting; subsequent removal of added currents returns default mSOD1 behavior ( right red ) in this neuron; Lower green trace shows I dyn . c. Time series plots of IEIs (log scale) for the three different conditions in ( b ); each dot represents an interval between two consecutive spikes (see detailed results). d. Autocorrelation function of the membrane voltage for default mSOD1 ( left ) and with addition of Nav1.6 currents (middle); the height of the 2 nd autocorrelation peak highlights rhythmicity (arrowhead); Right: measured 2 nd peak values for 4 mSOD1 cells are shown, with dashed horizontal line showing average WT values and shaded grey region indicates ±SD. e. Treatment effects on burst characteristics including burst duration (BD), inter-burst-intervals (IBI) and inter-spike-intervals within bursts (ISI) shown for various mSOD1 cells tested under the different conditions as in ( b ); dashed lines indicate average WT values with grey regions marking the ± s.d.

    Techniques Used: Introduce, Patch Clamp, Generated

    Immunofluorescent quantification of Nav1.6 protein expression in WT and mSOD1 Mes V cells. a) Representative images showing three rostro-caudal levels of coronal brainstem sections, stained with NeuN (left column of images); scalebar shows 500 μm. In these images, the Mes V nucleus is highlighted with dashed contours. At Level B, the subjacent trigeminal motor nucleus (mot V) is highlighted. For each level, comparative images consisting of Mes V neurons from WT and mSOD1 mice are shown at 60x magnification; green is NeuN, red is Nav1.6 protein. Merged images are shown enlarged with white arrows highlighting representative Mes V neurons. Left bottom boxed inset illustrates the pontine levels at which sections were collected. b) Representative image showing regions of interest (ROIs) drawn around 12 Mes V neurons for immunofluorescence quantification; white arrows highlight three representative ROIs c) Scatterplot showing WT (black circles) and mSOD1 (red circles) values of ROI area and mean intensity quantification. Trendlines show a positive linear regression. d) Box plots show mean ROI intensity per cell for WT (black) and mSOD1 (red) respectively; n values indicate number of cells obtained from 4 WT and 4 mSOD1 mice across 9 and 8 sections respectively. A two-tailed Student t-test was used for statistical comparison.
    Figure Legend Snippet: Immunofluorescent quantification of Nav1.6 protein expression in WT and mSOD1 Mes V cells. a) Representative images showing three rostro-caudal levels of coronal brainstem sections, stained with NeuN (left column of images); scalebar shows 500 μm. In these images, the Mes V nucleus is highlighted with dashed contours. At Level B, the subjacent trigeminal motor nucleus (mot V) is highlighted. For each level, comparative images consisting of Mes V neurons from WT and mSOD1 mice are shown at 60x magnification; green is NeuN, red is Nav1.6 protein. Merged images are shown enlarged with white arrows highlighting representative Mes V neurons. Left bottom boxed inset illustrates the pontine levels at which sections were collected. b) Representative image showing regions of interest (ROIs) drawn around 12 Mes V neurons for immunofluorescence quantification; white arrows highlight three representative ROIs c) Scatterplot showing WT (black circles) and mSOD1 (red circles) values of ROI area and mean intensity quantification. Trendlines show a positive linear regression. d) Box plots show mean ROI intensity per cell for WT (black) and mSOD1 (red) respectively; n values indicate number of cells obtained from 4 WT and 4 mSOD1 mice across 9 and 8 sections respectively. A two-tailed Student t-test was used for statistical comparison.

    Techniques Used: Expressing, Staining, Mouse Assay, Immunofluorescence, Two Tailed Test

    Related Articles

    Incubation:

    Article Title: Fetal brain hypometabolism during prolonged hypoxaemia in the llama
    Article Snippet: .. The blots were blocked with 4% nonfat milk in phosphate-buffered saline (PBS), and then incubated with one of the following primary antibodies: polyclonal anti-NaV1.1, anti-NaV1.2, anti-NaV1.3, anti-NaV1.6 (all from Alomone Laboratories, Jerusalem, Israel; used at 1:200 dilution), monoclonal antiβ-actin (Clone AC-74; Sigma, St Louis, MO, USA; used at a dilution of 1:5000), or monoclonal anti-PARP (Clone C2-10; BD PharMingen, San Diego, CA, USA; used at a dilution of 1:2000), followed by incubation with HRP-conjugated anti-rabbit IgG or anti-mouse IgG secondary antibodies (both from Jackson ImmunoResearch Laboratories, Inc., PA, USA; used at 1:5000 dilution) with washes with PBS/0.1% Tween 20 between primary and secondary antibody incubation. ..

    Article Title: Circuit-Specific Early Impairment of Proprioceptive Sensory Neurons in the SOD1G93A Mouse Model for ALS
    Article Snippet: .. Red fluorescence-conjugated Nav1.6 primary antibody derived from rabbit (Alomone Lab) was added (1:200 dilution) and incubated for 24 hours at 40 C. Appropriate dilution was determined based on initial dilution series experiments (1:100, 1:200 and 1:500) with suitable negative controls. ..

    Article Title: Functional modulation of voltage-dependent sodium channel expression by wild type and mutated C121W-β1 subunit.
    Article Snippet: .. Voltage dependent sodium channels are membrane proteins essential for cell excitability.. They are composed by a pore-forming α-subunit, encoded in mammals by up to 9 different genes, and 4 different ancillary β-subunits. ..

    Fluorescence:

    Article Title: Circuit-Specific Early Impairment of Proprioceptive Sensory Neurons in the SOD1G93A Mouse Model for ALS
    Article Snippet: .. Red fluorescence-conjugated Nav1.6 primary antibody derived from rabbit (Alomone Lab) was added (1:200 dilution) and incubated for 24 hours at 40 C. Appropriate dilution was determined based on initial dilution series experiments (1:100, 1:200 and 1:500) with suitable negative controls. ..

    Derivative Assay:

    Article Title: Circuit-Specific Early Impairment of Proprioceptive Sensory Neurons in the SOD1G93A Mouse Model for ALS
    Article Snippet: .. Red fluorescence-conjugated Nav1.6 primary antibody derived from rabbit (Alomone Lab) was added (1:200 dilution) and incubated for 24 hours at 40 C. Appropriate dilution was determined based on initial dilution series experiments (1:100, 1:200 and 1:500) with suitable negative controls. ..

    Transfection:

    Article Title: Functional modulation of voltage-dependent sodium channel expression by wild type and mutated C121W-β1 subunit.
    Article Snippet: .. Voltage dependent sodium channels are membrane proteins essential for cell excitability.. They are composed by a pore-forming α-subunit, encoded in mammals by up to 9 different genes, and 4 different ancillary β-subunits. ..

    Mutagenesis:

    Article Title: Functional modulation of voltage-dependent sodium channel expression by wild type and mutated C121W-β1 subunit.
    Article Snippet: .. Voltage dependent sodium channels are membrane proteins essential for cell excitability.. They are composed by a pore-forming α-subunit, encoded in mammals by up to 9 different genes, and 4 different ancillary β-subunits. ..

    Construct:

    Article Title: Functional modulation of voltage-dependent sodium channel expression by wild type and mutated C121W-β1 subunit.
    Article Snippet: .. Voltage dependent sodium channels are membrane proteins essential for cell excitability.. They are composed by a pore-forming α-subunit, encoded in mammals by up to 9 different genes, and 4 different ancillary β-subunits. ..

    Western Blot:

    Article Title: Antisense-mediated post-transcriptional silencing of SCN1B gene modulates sodium channel functional expression.
    Article Snippet: .. Voltage-dependent sodium channels are membrane proteins essential for cell excitability.. They are composed by a pore-forming α-subunit and one or more β subunits. ..

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  • 92
    Alomone Labs red fluorescence conjugated nav1 6 primary antibody
    Rescue of Nav 1.6-type sodium currents in mSOD1 Mes V neurons using dynamic-clamp. a. Schematic shows the dynamic-clamp setup used to introduce conductance-based models of <t>Nav1.6-type</t> Na + currents into mSOD1 Mes V neurons in real-time during whole-cell patch-clamp recording; I dyn is the computer-generated model Na + current in combination with a step depolarization to drive the patched Mes V neuron; V m is the measured membrane voltage. b. Representative traces showing the membrane voltage in a bursting mSOD1 Mes V neuron with control/default behavior ( left red ), followed by addition of Nav1.6-type currents ( middle black ), that restores WT-like rhythmic bursting; subsequent removal of added currents returns default mSOD1 behavior ( right red ) in this neuron; Lower green trace shows I dyn . c. Time series plots of IEIs (log scale) for the three different conditions in ( b ); each dot represents an interval between two consecutive spikes (see detailed results). d. Autocorrelation function of the membrane voltage for default mSOD1 ( left ) and with addition of Nav1.6 currents (middle); the height of the 2 nd autocorrelation peak highlights rhythmicity (arrowhead); Right: measured 2 nd peak values for 4 mSOD1 cells are shown, with dashed horizontal line showing average WT values and shaded grey region indicates ±SD. e. Treatment effects on burst characteristics including burst duration (BD), inter-burst-intervals (IBI) and inter-spike-intervals within bursts (ISI) shown for various mSOD1 cells tested under the different conditions as in ( b ); dashed lines indicate average WT values with grey regions marking the ± s.d.
    Red Fluorescence Conjugated Nav1 6 Primary Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/red fluorescence conjugated nav1 6 primary antibody/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    red fluorescence conjugated nav1 6 primary antibody - by Bioz Stars, 2021-09
    92/100 stars
      Buy from Supplier

    Image Search Results


    Rescue of Nav 1.6-type sodium currents in mSOD1 Mes V neurons using dynamic-clamp. a. Schematic shows the dynamic-clamp setup used to introduce conductance-based models of Nav1.6-type Na + currents into mSOD1 Mes V neurons in real-time during whole-cell patch-clamp recording; I dyn is the computer-generated model Na + current in combination with a step depolarization to drive the patched Mes V neuron; V m is the measured membrane voltage. b. Representative traces showing the membrane voltage in a bursting mSOD1 Mes V neuron with control/default behavior ( left red ), followed by addition of Nav1.6-type currents ( middle black ), that restores WT-like rhythmic bursting; subsequent removal of added currents returns default mSOD1 behavior ( right red ) in this neuron; Lower green trace shows I dyn . c. Time series plots of IEIs (log scale) for the three different conditions in ( b ); each dot represents an interval between two consecutive spikes (see detailed results). d. Autocorrelation function of the membrane voltage for default mSOD1 ( left ) and with addition of Nav1.6 currents (middle); the height of the 2 nd autocorrelation peak highlights rhythmicity (arrowhead); Right: measured 2 nd peak values for 4 mSOD1 cells are shown, with dashed horizontal line showing average WT values and shaded grey region indicates ±SD. e. Treatment effects on burst characteristics including burst duration (BD), inter-burst-intervals (IBI) and inter-spike-intervals within bursts (ISI) shown for various mSOD1 cells tested under the different conditions as in ( b ); dashed lines indicate average WT values with grey regions marking the ± s.d.

    Journal: bioRxiv

    Article Title: Circuit-Specific Early Impairment of Proprioceptive Sensory Neurons in the SOD1G93A Mouse Model for ALS

    doi: 10.1101/669788

    Figure Lengend Snippet: Rescue of Nav 1.6-type sodium currents in mSOD1 Mes V neurons using dynamic-clamp. a. Schematic shows the dynamic-clamp setup used to introduce conductance-based models of Nav1.6-type Na + currents into mSOD1 Mes V neurons in real-time during whole-cell patch-clamp recording; I dyn is the computer-generated model Na + current in combination with a step depolarization to drive the patched Mes V neuron; V m is the measured membrane voltage. b. Representative traces showing the membrane voltage in a bursting mSOD1 Mes V neuron with control/default behavior ( left red ), followed by addition of Nav1.6-type currents ( middle black ), that restores WT-like rhythmic bursting; subsequent removal of added currents returns default mSOD1 behavior ( right red ) in this neuron; Lower green trace shows I dyn . c. Time series plots of IEIs (log scale) for the three different conditions in ( b ); each dot represents an interval between two consecutive spikes (see detailed results). d. Autocorrelation function of the membrane voltage for default mSOD1 ( left ) and with addition of Nav1.6 currents (middle); the height of the 2 nd autocorrelation peak highlights rhythmicity (arrowhead); Right: measured 2 nd peak values for 4 mSOD1 cells are shown, with dashed horizontal line showing average WT values and shaded grey region indicates ±SD. e. Treatment effects on burst characteristics including burst duration (BD), inter-burst-intervals (IBI) and inter-spike-intervals within bursts (ISI) shown for various mSOD1 cells tested under the different conditions as in ( b ); dashed lines indicate average WT values with grey regions marking the ± s.d.

    Article Snippet: Red fluorescence-conjugated Nav1.6 primary antibody derived from rabbit (Alomone Lab) was added (1:200 dilution) and incubated for 24 hours at 40 C. Appropriate dilution was determined based on initial dilution series experiments (1:100, 1:200 and 1:500) with suitable negative controls.

    Techniques: Introduce, Patch Clamp, Generated

    Immunofluorescent quantification of Nav1.6 protein expression in WT and mSOD1 Mes V cells. a) Representative images showing three rostro-caudal levels of coronal brainstem sections, stained with NeuN (left column of images); scalebar shows 500 μm. In these images, the Mes V nucleus is highlighted with dashed contours. At Level B, the subjacent trigeminal motor nucleus (mot V) is highlighted. For each level, comparative images consisting of Mes V neurons from WT and mSOD1 mice are shown at 60x magnification; green is NeuN, red is Nav1.6 protein. Merged images are shown enlarged with white arrows highlighting representative Mes V neurons. Left bottom boxed inset illustrates the pontine levels at which sections were collected. b) Representative image showing regions of interest (ROIs) drawn around 12 Mes V neurons for immunofluorescence quantification; white arrows highlight three representative ROIs c) Scatterplot showing WT (black circles) and mSOD1 (red circles) values of ROI area and mean intensity quantification. Trendlines show a positive linear regression. d) Box plots show mean ROI intensity per cell for WT (black) and mSOD1 (red) respectively; n values indicate number of cells obtained from 4 WT and 4 mSOD1 mice across 9 and 8 sections respectively. A two-tailed Student t-test was used for statistical comparison.

    Journal: bioRxiv

    Article Title: Circuit-Specific Early Impairment of Proprioceptive Sensory Neurons in the SOD1G93A Mouse Model for ALS

    doi: 10.1101/669788

    Figure Lengend Snippet: Immunofluorescent quantification of Nav1.6 protein expression in WT and mSOD1 Mes V cells. a) Representative images showing three rostro-caudal levels of coronal brainstem sections, stained with NeuN (left column of images); scalebar shows 500 μm. In these images, the Mes V nucleus is highlighted with dashed contours. At Level B, the subjacent trigeminal motor nucleus (mot V) is highlighted. For each level, comparative images consisting of Mes V neurons from WT and mSOD1 mice are shown at 60x magnification; green is NeuN, red is Nav1.6 protein. Merged images are shown enlarged with white arrows highlighting representative Mes V neurons. Left bottom boxed inset illustrates the pontine levels at which sections were collected. b) Representative image showing regions of interest (ROIs) drawn around 12 Mes V neurons for immunofluorescence quantification; white arrows highlight three representative ROIs c) Scatterplot showing WT (black circles) and mSOD1 (red circles) values of ROI area and mean intensity quantification. Trendlines show a positive linear regression. d) Box plots show mean ROI intensity per cell for WT (black) and mSOD1 (red) respectively; n values indicate number of cells obtained from 4 WT and 4 mSOD1 mice across 9 and 8 sections respectively. A two-tailed Student t-test was used for statistical comparison.

    Article Snippet: Red fluorescence-conjugated Nav1.6 primary antibody derived from rabbit (Alomone Lab) was added (1:200 dilution) and incubated for 24 hours at 40 C. Appropriate dilution was determined based on initial dilution series experiments (1:100, 1:200 and 1:500) with suitable negative controls.

    Techniques: Expressing, Staining, Mouse Assay, Immunofluorescence, Two Tailed Test