glt1  (Alomone Labs)


Bioz Verified Symbol Alomone Labs is a verified supplier
Bioz Manufacturer Symbol Alomone Labs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92

    Structured Review

    Alomone Labs glt1
    Adeno-associated virus (AAV)-mediated deletion of Per2 in glial cells of adult mice leads to a depression resistant behavior. (A) Fluorescent imaging of whole brains 3 weeks after no injection (left), intravenous (i.v.) injection of the engineered AAV-PHP.eB, which can pass the blood-brain barrier (BBB), containing the general CAG driver (second from left) or the glial Gfap driver (middle). The second to last brain is from an animal with i.v. injected AAV-9, which does not pass the BBB, containing the general CAG driver. The last brain (right) is from an animal injected intraperitoneally (i.p.) with the AAV-PHP.eB Gfap -driven construct. Note that only the brains of animals that received the AAV-PHP.eb i.v. display significant fluorescent signal after 3 weeks (orange and yellow color). (B) Fluorescent imaging of whole brains 2 months after injection of the AAV-PHP.eB. Note that the fluorescence is still maintained after 2 months post injection and that even the i.p. injected AAV-PHP.eB Gfap is showing signal in the brain now. (C) Sorting of neurons and astrocytes by flow cytometry from brain tissue including the nucleus accumbens (NAc). The left panel shows the removal of debris from a single cell suspension, showing the distribution of debris in the forward as well as in the side scatter (FSC and SSC, respectively). The middle panel shows the removal of CD11b + cells (microglia) from the cell suspension (lower left corner from left panel). The CD11b - cells (bottom half from middle panel) were then sorted into two distinct cell populations corresponding to astrocytes <t>(GLT1</t> + /CD90.2 - ) and neurons (CD90.2 + /GLT1 - ) (right panel). (D) PCR analysis of astrocytes and neurons from the cell sorting. Microglia (CD11 + ) as well as astrocytes (GLT1 + /CD90.2 - ), but not neurons (CD90.2 + /GLT1 - ) from PHP.eB Gfap-iCre infected animals show the presence of iCre , indicating that only glia and not neurons could express iCre in order to delete Per2 in the Per2 fl/fl mice. (E) Immunohistochemistry of vG Per2 brain tissue from nucleus accumbens (NAc) isolated at ZT6. The signal for PER2 (green) mainly overlaps with neuronal NeuN signal (red) giving rise to the yellow color. Scale bar: 100 µm. (F) Immobility time in the forced swim test (FST) of vG Per2 (PHP.eB Gfap-iCre , green) and control (PHP.eB control, blue) animals are shown (n = 5, two-tailed t-test, *p
    Glt1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glt1/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    glt1 - by Bioz Stars, 2022-05
    92/100 stars

    Images

    1) Product Images from "Deletion of the Clock Gene Period2 (Per2) in Glial Cells Alters Mood-Related Behavior in Mice"

    Article Title: Deletion of the Clock Gene Period2 (Per2) in Glial Cells Alters Mood-Related Behavior in Mice

    Journal: bioRxiv

    doi: 10.1101/2020.12.09.417162

    Adeno-associated virus (AAV)-mediated deletion of Per2 in glial cells of adult mice leads to a depression resistant behavior. (A) Fluorescent imaging of whole brains 3 weeks after no injection (left), intravenous (i.v.) injection of the engineered AAV-PHP.eB, which can pass the blood-brain barrier (BBB), containing the general CAG driver (second from left) or the glial Gfap driver (middle). The second to last brain is from an animal with i.v. injected AAV-9, which does not pass the BBB, containing the general CAG driver. The last brain (right) is from an animal injected intraperitoneally (i.p.) with the AAV-PHP.eB Gfap -driven construct. Note that only the brains of animals that received the AAV-PHP.eb i.v. display significant fluorescent signal after 3 weeks (orange and yellow color). (B) Fluorescent imaging of whole brains 2 months after injection of the AAV-PHP.eB. Note that the fluorescence is still maintained after 2 months post injection and that even the i.p. injected AAV-PHP.eB Gfap is showing signal in the brain now. (C) Sorting of neurons and astrocytes by flow cytometry from brain tissue including the nucleus accumbens (NAc). The left panel shows the removal of debris from a single cell suspension, showing the distribution of debris in the forward as well as in the side scatter (FSC and SSC, respectively). The middle panel shows the removal of CD11b + cells (microglia) from the cell suspension (lower left corner from left panel). The CD11b - cells (bottom half from middle panel) were then sorted into two distinct cell populations corresponding to astrocytes (GLT1 + /CD90.2 - ) and neurons (CD90.2 + /GLT1 - ) (right panel). (D) PCR analysis of astrocytes and neurons from the cell sorting. Microglia (CD11 + ) as well as astrocytes (GLT1 + /CD90.2 - ), but not neurons (CD90.2 + /GLT1 - ) from PHP.eB Gfap-iCre infected animals show the presence of iCre , indicating that only glia and not neurons could express iCre in order to delete Per2 in the Per2 fl/fl mice. (E) Immunohistochemistry of vG Per2 brain tissue from nucleus accumbens (NAc) isolated at ZT6. The signal for PER2 (green) mainly overlaps with neuronal NeuN signal (red) giving rise to the yellow color. Scale bar: 100 µm. (F) Immobility time in the forced swim test (FST) of vG Per2 (PHP.eB Gfap-iCre , green) and control (PHP.eB control, blue) animals are shown (n = 5, two-tailed t-test, *p
    Figure Legend Snippet: Adeno-associated virus (AAV)-mediated deletion of Per2 in glial cells of adult mice leads to a depression resistant behavior. (A) Fluorescent imaging of whole brains 3 weeks after no injection (left), intravenous (i.v.) injection of the engineered AAV-PHP.eB, which can pass the blood-brain barrier (BBB), containing the general CAG driver (second from left) or the glial Gfap driver (middle). The second to last brain is from an animal with i.v. injected AAV-9, which does not pass the BBB, containing the general CAG driver. The last brain (right) is from an animal injected intraperitoneally (i.p.) with the AAV-PHP.eB Gfap -driven construct. Note that only the brains of animals that received the AAV-PHP.eb i.v. display significant fluorescent signal after 3 weeks (orange and yellow color). (B) Fluorescent imaging of whole brains 2 months after injection of the AAV-PHP.eB. Note that the fluorescence is still maintained after 2 months post injection and that even the i.p. injected AAV-PHP.eB Gfap is showing signal in the brain now. (C) Sorting of neurons and astrocytes by flow cytometry from brain tissue including the nucleus accumbens (NAc). The left panel shows the removal of debris from a single cell suspension, showing the distribution of debris in the forward as well as in the side scatter (FSC and SSC, respectively). The middle panel shows the removal of CD11b + cells (microglia) from the cell suspension (lower left corner from left panel). The CD11b - cells (bottom half from middle panel) were then sorted into two distinct cell populations corresponding to astrocytes (GLT1 + /CD90.2 - ) and neurons (CD90.2 + /GLT1 - ) (right panel). (D) PCR analysis of astrocytes and neurons from the cell sorting. Microglia (CD11 + ) as well as astrocytes (GLT1 + /CD90.2 - ), but not neurons (CD90.2 + /GLT1 - ) from PHP.eB Gfap-iCre infected animals show the presence of iCre , indicating that only glia and not neurons could express iCre in order to delete Per2 in the Per2 fl/fl mice. (E) Immunohistochemistry of vG Per2 brain tissue from nucleus accumbens (NAc) isolated at ZT6. The signal for PER2 (green) mainly overlaps with neuronal NeuN signal (red) giving rise to the yellow color. Scale bar: 100 µm. (F) Immobility time in the forced swim test (FST) of vG Per2 (PHP.eB Gfap-iCre , green) and control (PHP.eB control, blue) animals are shown (n = 5, two-tailed t-test, *p

    Techniques Used: Mouse Assay, Imaging, Injection, Construct, Fluorescence, Flow Cytometry, Polymerase Chain Reaction, FACS, Infection, Immunohistochemistry, Isolation, Two Tailed Test

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Alomone Labs anti kcnq1
    The chronic effect of PMA on I Ks current in HEK 293B cell. (a) The representative I Ks current under the PMA (100 nM) treatment. (b) The current–voltage relationship for the tail currents under the PMA (100 nM) treatment. (c) The summary data for the tail currents under the PMA (100 nM) treatment at +40 mV prepulse. (d) The normalized I–V relationship for I Ks current. The solid lines represent fits to a Boltzmann function. (e) The representative immunoblot and summary data for the <t>KCNQ1</t> channel membrane protein under the PMA (100 nM) treatment (** P
    Anti Kcnq1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti kcnq1/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti kcnq1 - by Bioz Stars, 2022-05
    93/100 stars
      Buy from Supplier

    94
    Alomone Labs cav1 2
    <t>Cav1.2</t> knock-down prevents astrocyte activation after scratch
    Cav1 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cav1 2/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cav1 2 - by Bioz Stars, 2022-05
    94/100 stars
      Buy from Supplier

    Image Search Results


    The chronic effect of PMA on I Ks current in HEK 293B cell. (a) The representative I Ks current under the PMA (100 nM) treatment. (b) The current–voltage relationship for the tail currents under the PMA (100 nM) treatment. (c) The summary data for the tail currents under the PMA (100 nM) treatment at +40 mV prepulse. (d) The normalized I–V relationship for I Ks current. The solid lines represent fits to a Boltzmann function. (e) The representative immunoblot and summary data for the KCNQ1 channel membrane protein under the PMA (100 nM) treatment (** P

    Journal: Channels

    Article Title: Specific protein kinase C isoform exerts chronic inhibition on the slowly activating delayed-rectifier potassium current by affecting channel trafficking

    doi: 10.1080/19336950.2021.1882112

    Figure Lengend Snippet: The chronic effect of PMA on I Ks current in HEK 293B cell. (a) The representative I Ks current under the PMA (100 nM) treatment. (b) The current–voltage relationship for the tail currents under the PMA (100 nM) treatment. (c) The summary data for the tail currents under the PMA (100 nM) treatment at +40 mV prepulse. (d) The normalized I–V relationship for I Ks current. The solid lines represent fits to a Boltzmann function. (e) The representative immunoblot and summary data for the KCNQ1 channel membrane protein under the PMA (100 nM) treatment (** P

    Article Snippet: The following primary antibodies were used as follows: anti-Na/K-ATPase (Proteintech, 1:500) and anti-KCNQ1 (Alomone, 1:700).

    Techniques:

    The chronic effect of Ang II on I Ks current in HEK 293B cell. (a) The representative I Ks current evoked by voltage protocol shown in inset under the Ang II (100 nM) treatment. (b) The current–voltage relationship for the tail currents under the Ang II (100 nM) treatment. (c) The summary data for the tail currents under the Ang II (100 nM) treatment at +40 mV prepulse. (d) The normalized I–V relationship for I Ks current. The solid lines represent fits to a Boltzmann function. (e) The representative immunoblot and summary data for the KCNQ1 channel membrane protein under the Ang II (100 nM) treatment (** P

    Journal: Channels

    Article Title: Specific protein kinase C isoform exerts chronic inhibition on the slowly activating delayed-rectifier potassium current by affecting channel trafficking

    doi: 10.1080/19336950.2021.1882112

    Figure Lengend Snippet: The chronic effect of Ang II on I Ks current in HEK 293B cell. (a) The representative I Ks current evoked by voltage protocol shown in inset under the Ang II (100 nM) treatment. (b) The current–voltage relationship for the tail currents under the Ang II (100 nM) treatment. (c) The summary data for the tail currents under the Ang II (100 nM) treatment at +40 mV prepulse. (d) The normalized I–V relationship for I Ks current. The solid lines represent fits to a Boltzmann function. (e) The representative immunoblot and summary data for the KCNQ1 channel membrane protein under the Ang II (100 nM) treatment (** P

    Article Snippet: The following primary antibodies were used as follows: anti-Na/K-ATPase (Proteintech, 1:500) and anti-KCNQ1 (Alomone, 1:700).

    Techniques:

    Effects of enalapril (ENA) or losartan (LOS) on the decreased protein expression of I Ks subunits KCNQ1 and KCNE1 by aldosterone (ALD). Cells were incubated with aldosterone (1 μmol·L -1 ) alone or accompanied with enalapril (1 μmol·L -1 ), losartan (1 μmol·L -1 ) for 24 h. We used western blot to assess I Ks subunits KCNQ1 and KCNE1. A. Representative immunoblots for KCNQ1, KCNE1 proteins along with internal standard GAPDH. B. Mean ± SEM expression levels presented as fold change compared to the control group’s mean value and the quantification of the band intensities were normalized with GAPDH ( n = 3). ** P

    Journal: American Journal of Translational Research

    Article Title: Aldosterone downregulates delayed rectifier potassium currents through an angiotensin type 1 receptor-dependent mechanism

    doi:

    Figure Lengend Snippet: Effects of enalapril (ENA) or losartan (LOS) on the decreased protein expression of I Ks subunits KCNQ1 and KCNE1 by aldosterone (ALD). Cells were incubated with aldosterone (1 μmol·L -1 ) alone or accompanied with enalapril (1 μmol·L -1 ), losartan (1 μmol·L -1 ) for 24 h. We used western blot to assess I Ks subunits KCNQ1 and KCNE1. A. Representative immunoblots for KCNQ1, KCNE1 proteins along with internal standard GAPDH. B. Mean ± SEM expression levels presented as fold change compared to the control group’s mean value and the quantification of the band intensities were normalized with GAPDH ( n = 3). ** P

    Article Snippet: Antibodies were anti-GAPDH (Proteintech, China), anti-KCNQ1 (Alomone lab, Israel), anti-KCNE1 (Alomone lab, Israel).

    Techniques: Expressing, Incubation, Western Blot

    Characterization of the KCNQ1 concatenated tetrameric construct without and with KCNE1. A , top panel , scheme of the KCNQ1 concatenated tetrameric channel construct (Con′), where subunits D 1 , D 2 , D 3 , and D 4 are connected by flexible linkers. Each

    Journal: The Journal of Biological Chemistry

    Article Title: KCNQ1 Channels Do Not Undergo Concerted but Sequential Gating Transitions in Both the Absence and the Presence of KCNE1 Protein *

    doi: 10.1074/jbc.M112.364901

    Figure Lengend Snippet: Characterization of the KCNQ1 concatenated tetrameric construct without and with KCNE1. A , top panel , scheme of the KCNQ1 concatenated tetrameric channel construct (Con′), where subunits D 1 , D 2 , D 3 , and D 4 are connected by flexible linkers. Each

    Article Snippet: Equal amounts of lysate proteins were resolved by 8% SDS-PAGE, and blots were reacted using rabbit anti-KCNQ1 antibodies (Alomone Labs).

    Techniques: Construct

    R231W-bearing KCNQ1 subunits in the presence of KCNE1 produce a gradual left-shift in the voltage dependence of activation. A , representative current traces of Con′, with one, two, three, and four subunits harboring the R231W mutation, in the

    Journal: The Journal of Biological Chemistry

    Article Title: KCNQ1 Channels Do Not Undergo Concerted but Sequential Gating Transitions in Both the Absence and the Presence of KCNE1 Protein *

    doi: 10.1074/jbc.M112.364901

    Figure Lengend Snippet: R231W-bearing KCNQ1 subunits in the presence of KCNE1 produce a gradual left-shift in the voltage dependence of activation. A , representative current traces of Con′, with one, two, three, and four subunits harboring the R231W mutation, in the

    Article Snippet: Equal amounts of lysate proteins were resolved by 8% SDS-PAGE, and blots were reacted using rabbit anti-KCNQ1 antibodies (Alomone Labs).

    Techniques: Activation Assay, Mutagenesis

    R231W-bearing KCNQ1 subunits in the presence of KCNE1 produce a gradual increase in the instantaneous component of the current and in the fractional constitutive conductance. A , representative traces of Con′ harboring the indicated combinations

    Journal: The Journal of Biological Chemistry

    Article Title: KCNQ1 Channels Do Not Undergo Concerted but Sequential Gating Transitions in Both the Absence and the Presence of KCNE1 Protein *

    doi: 10.1074/jbc.M112.364901

    Figure Lengend Snippet: R231W-bearing KCNQ1 subunits in the presence of KCNE1 produce a gradual increase in the instantaneous component of the current and in the fractional constitutive conductance. A , representative traces of Con′ harboring the indicated combinations

    Article Snippet: Equal amounts of lysate proteins were resolved by 8% SDS-PAGE, and blots were reacted using rabbit anti-KCNQ1 antibodies (Alomone Labs).

    Techniques:

    Each KCNQ1 Subunit Endowed with a Gain of Function Mutation in the VSD Produces an Incremental Gating Perturbation

    Journal: The Journal of Biological Chemistry

    Article Title: KCNQ1 Channels Do Not Undergo Concerted but Sequential Gating Transitions in Both the Absence and the Presence of KCNE1 Protein *

    doi: 10.1074/jbc.M112.364901

    Figure Lengend Snippet: Each KCNQ1 Subunit Endowed with a Gain of Function Mutation in the VSD Produces an Incremental Gating Perturbation

    Article Snippet: Equal amounts of lysate proteins were resolved by 8% SDS-PAGE, and blots were reacted using rabbit anti-KCNQ1 antibodies (Alomone Labs).

    Techniques: Mutagenesis

    R243W-bearing KCNQ1 subunits produce a gradual right-shift in the voltage dependence of activation. A and C , representative current traces of Con′, with the indicated combinations of subunits harboring the R243W mutation in the absence ( A ) or

    Journal: The Journal of Biological Chemistry

    Article Title: KCNQ1 Channels Do Not Undergo Concerted but Sequential Gating Transitions in Both the Absence and the Presence of KCNE1 Protein *

    doi: 10.1074/jbc.M112.364901

    Figure Lengend Snippet: R243W-bearing KCNQ1 subunits produce a gradual right-shift in the voltage dependence of activation. A and C , representative current traces of Con′, with the indicated combinations of subunits harboring the R243W mutation in the absence ( A ) or

    Article Snippet: Equal amounts of lysate proteins were resolved by 8% SDS-PAGE, and blots were reacted using rabbit anti-KCNQ1 antibodies (Alomone Labs).

    Techniques: Activation Assay, Mutagenesis

    R231W-bearing KCNQ1 subunits produce a gradual left-shift in the voltage dependence of activation. A , representative current traces of Con′, with one, two, three, and four subunits harboring the R231W mutation. CHO cells were held at −90

    Journal: The Journal of Biological Chemistry

    Article Title: KCNQ1 Channels Do Not Undergo Concerted but Sequential Gating Transitions in Both the Absence and the Presence of KCNE1 Protein *

    doi: 10.1074/jbc.M112.364901

    Figure Lengend Snippet: R231W-bearing KCNQ1 subunits produce a gradual left-shift in the voltage dependence of activation. A , representative current traces of Con′, with one, two, three, and four subunits harboring the R231W mutation. CHO cells were held at −90

    Article Snippet: Equal amounts of lysate proteins were resolved by 8% SDS-PAGE, and blots were reacted using rabbit anti-KCNQ1 antibodies (Alomone Labs).

    Techniques: Activation Assay, Mutagenesis

    Each KCNQ1 Subunit Endowed with a Gain of Function Mutation in the VSD Produces an Incremental Gating Perturbation

    Journal: The Journal of Biological Chemistry

    Article Title: KCNQ1 Channels Do Not Undergo Concerted but Sequential Gating Transitions in Both the Absence and the Presence of KCNE1 Protein *

    doi: 10.1074/jbc.M112.364901

    Figure Lengend Snippet: Each KCNQ1 Subunit Endowed with a Gain of Function Mutation in the VSD Produces an Incremental Gating Perturbation

    Article Snippet: Equal amounts of lysate proteins were resolved by 8% SDS-PAGE, and blots were reacted using rabbit anti-KCNQ1 antibodies (Alomone Labs).

    Techniques: Mutagenesis

    L353K-bearing KCNQ1 subunits produce a gradual left-shift in the voltage dependence of activation. A , representative current traces of Con′, with one, two, three, and four subunits harboring the L353K mutation. The membrane voltage was stepped

    Journal: The Journal of Biological Chemistry

    Article Title: KCNQ1 Channels Do Not Undergo Concerted but Sequential Gating Transitions in Both the Absence and the Presence of KCNE1 Protein *

    doi: 10.1074/jbc.M112.364901

    Figure Lengend Snippet: L353K-bearing KCNQ1 subunits produce a gradual left-shift in the voltage dependence of activation. A , representative current traces of Con′, with one, two, three, and four subunits harboring the L353K mutation. The membrane voltage was stepped

    Article Snippet: Equal amounts of lysate proteins were resolved by 8% SDS-PAGE, and blots were reacted using rabbit anti-KCNQ1 antibodies (Alomone Labs).

    Techniques: Activation Assay, Mutagenesis

    Each KCNQ1 Subunit Endowed with a Gain of Function Mutation in the VSD Produces an Incremental Gating Perturbation

    Journal: The Journal of Biological Chemistry

    Article Title: KCNQ1 Channels Do Not Undergo Concerted but Sequential Gating Transitions in Both the Absence and the Presence of KCNE1 Protein *

    doi: 10.1074/jbc.M112.364901

    Figure Lengend Snippet: Each KCNQ1 Subunit Endowed with a Gain of Function Mutation in the VSD Produces an Incremental Gating Perturbation

    Article Snippet: Equal amounts of lysate proteins were resolved by 8% SDS-PAGE, and blots were reacted using rabbit anti-KCNQ1 antibodies (Alomone Labs).

    Techniques: Mutagenesis

    R231W-bearing KCNQ1 subunits produce a gradual increase in the instantaneous component of the current and in the fractional constitutive conductance. A , representative traces of Con′ harboring the indicated combinations of R231W mutated subunits.

    Journal: The Journal of Biological Chemistry

    Article Title: KCNQ1 Channels Do Not Undergo Concerted but Sequential Gating Transitions in Both the Absence and the Presence of KCNE1 Protein *

    doi: 10.1074/jbc.M112.364901

    Figure Lengend Snippet: R231W-bearing KCNQ1 subunits produce a gradual increase in the instantaneous component of the current and in the fractional constitutive conductance. A , representative traces of Con′ harboring the indicated combinations of R231W mutated subunits.

    Article Snippet: Equal amounts of lysate proteins were resolved by 8% SDS-PAGE, and blots were reacted using rabbit anti-KCNQ1 antibodies (Alomone Labs).

    Techniques:

    Cav1.2 knock-down prevents astrocyte activation after scratch

    Journal: Glia

    Article Title: L-type voltage-operated calcium channels contribute to astrocyte activation in vitro

    doi: 10.1002/glia.23013

    Figure Lengend Snippet: Cav1.2 knock-down prevents astrocyte activation after scratch

    Article Snippet: In contrast, RT-PCR, western blot and immunocytochemistry experiments show that Cav1.2 and Cav1.3 are highly expressed in cortical astrocytes ( ). shows representative images of labeled cultured astrocytes at 6 days in vitro ( DIV ); while GFAP abundantly labeled cell processes and cell bodies, labeling with Cav1.2 and Cav1.3 antibodies displayed a punctate staining on the cells surface ( ).

    Techniques: Activation Assay

    LPS enhance the activity and the expression of Cav1.2 Ca ++ channels in astrocytes

    Journal: Glia

    Article Title: L-type voltage-operated calcium channels contribute to astrocyte activation in vitro

    doi: 10.1002/glia.23013

    Figure Lengend Snippet: LPS enhance the activity and the expression of Cav1.2 Ca ++ channels in astrocytes

    Article Snippet: In contrast, RT-PCR, western blot and immunocytochemistry experiments show that Cav1.2 and Cav1.3 are highly expressed in cortical astrocytes ( ). shows representative images of labeled cultured astrocytes at 6 days in vitro ( DIV ); while GFAP abundantly labeled cell processes and cell bodies, labeling with Cav1.2 and Cav1.3 antibodies displayed a punctate staining on the cells surface ( ).

    Techniques: Activity Assay, Expressing

    Cav1.2 KO astrocytes are not sensitive to LPS

    Journal: Glia

    Article Title: L-type voltage-operated calcium channels contribute to astrocyte activation in vitro

    doi: 10.1002/glia.23013

    Figure Lengend Snippet: Cav1.2 KO astrocytes are not sensitive to LPS

    Article Snippet: In contrast, RT-PCR, western blot and immunocytochemistry experiments show that Cav1.2 and Cav1.3 are highly expressed in cortical astrocytes ( ). shows representative images of labeled cultured astrocytes at 6 days in vitro ( DIV ); while GFAP abundantly labeled cell processes and cell bodies, labeling with Cav1.2 and Cav1.3 antibodies displayed a punctate staining on the cells surface ( ).

    Techniques:

    Cav1.2 knock-down prevents astrocyte activation by LPS

    Journal: Glia

    Article Title: L-type voltage-operated calcium channels contribute to astrocyte activation in vitro

    doi: 10.1002/glia.23013

    Figure Lengend Snippet: Cav1.2 knock-down prevents astrocyte activation by LPS

    Article Snippet: In contrast, RT-PCR, western blot and immunocytochemistry experiments show that Cav1.2 and Cav1.3 are highly expressed in cortical astrocytes ( ). shows representative images of labeled cultured astrocytes at 6 days in vitro ( DIV ); while GFAP abundantly labeled cell processes and cell bodies, labeling with Cav1.2 and Cav1.3 antibodies displayed a punctate staining on the cells surface ( ).

    Techniques: Activation Assay