dsdna (Worthington Biochemical)

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DNA Cellulose Double Stranded

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Prepared by a method developed at Worthington in which native double stranded calf thymus DNA is covalently bound to cellulose Suitable for the purification of many DNA binding proteins such as polymerases transcription factors and terminators etc Supplied as a dry powder One gram of DNA cellulose will swell to 3 4ml when fully hydrated

Catalog Number:

ls01120

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Size:

1 gm

Source:

Calf Thymus

Cas Number:

9007.49.2

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Structured Review

Worthington Biochemical dsdna
$TCR revision upon repeated immunization with antigen. (A) TCR CDR3 length profiles of mice immunized 8× with PBS, 2× or 8× with SEB. TCR repertoire of splenic CD4 + T cell was skewed only after immunization 8× with SEB. (B) Expression of V(D)J recombinase complex and related molecules in the spleen of PBS- or SEB-injected BALB/c mice. (C) GFP + cells in the Vβ8 + CD4 + T population of rag1/gfp knock-in mice. <t>IgG-RF</t> as induced in rag1/gfp knock-in mice after immunization 8× with SEB (lower left). The GFP + T cell fraction was also increased among Vβ8 + CD4 + T cells (mean ± SD, 4–5 mice/group). (D) TCRα chain revision in the spleen of mice immunized 8× with SEB was determined by LM-PCR detection of <t>dsDNA</t> breaks at the RSS flanking the TCRAV2 , with PCR-amplified TCRα constant region ( TCRAC ) as a DNA quality control.$
Prepared by a method developed at Worthington in which native double stranded calf thymus DNA is covalently bound to cellulose Suitable for the purification of many DNA binding proteins such as polymerases transcription factors and terminators etc Supplied as a dry powder One gram of DNA cellulose will swell to 3 4ml when fully hydrated
https://www.bioz.com/result/dsdna/product/Worthington Biochemical
Average 94 stars, based on 194 article reviews
Price from $9.99 to $1999.99

dsdna - by Bioz Stars, 2021-01

94/100 stars

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Images

1) Product Images from "Self-Organized Criticality Theory of Autoimmunity"

Article Title: Self-Organized Criticality Theory of Autoimmunity

Journal: PLoS ONE

doi: 10.1371/journal.pone.0008382

$TCR revision upon repeated immunization with antigen. (A) TCR CDR3 length profiles of mice immunized 8× with PBS, 2× or 8× with SEB. TCR repertoire of splenic CD4 + T cell was skewed only after immunization 8× with SEB. (B) Expression of V(D)J recombinase complex and related molecules in the spleen of PBS- or SEB-injected BALB/c mice. (C) GFP + cells in the Vβ8 + CD4 + T population of rag1/gfp knock-in mice. IgG-RF as induced in rag1/gfp knock-in mice after immunization 8× with SEB (lower left). The GFP + T cell fraction was also increased among Vβ8 + CD4 + T cells (mean ± SD, 4–5 mice/group). (D) TCRα chain revision in the spleen of mice immunized 8× with SEB was determined by LM-PCR detection of dsDNA breaks at the RSS flanking the TCRAV2 , with PCR-amplified TCRα constant region ( TCRAC ) as a DNA quality control.$
Figure Legend Snippet: TCR revision upon repeated immunization with antigen. (A) TCR CDR3 length profiles of mice immunized 8× with PBS, 2× or 8× with SEB. TCR repertoire of splenic CD4 + T cell was skewed only after immunization 8× with SEB. (B) Expression of V(D)J recombinase complex and related molecules in the spleen of PBS- or SEB-injected BALB/c mice. (C) GFP + cells in the Vβ8 + CD4 + T population of rag1/gfp knock-in mice. IgG-RF as induced in rag1/gfp knock-in mice after immunization 8× with SEB (lower left). The GFP + T cell fraction was also increased among Vβ8 + CD4 + T cells (mean ± SD, 4–5 mice/group). (D) TCRα chain revision in the spleen of mice immunized 8× with SEB was determined by LM-PCR detection of dsDNA breaks at the RSS flanking the TCRAV2 , with PCR-amplified TCRα constant region ( TCRAC ) as a DNA quality control.

Techniques Used: Mouse Assay, Expressing, Injection, Knock-In, Polymerase Chain Reaction, Amplification

2) Product Images from "Carbonium vs. carbenium ion-like transition state geometries for carbocation cyclization - how strain associated with bridging affects 5-exo vs. 6-endo selectivity"

Article Title: Carbonium vs. carbenium ion-like transition state geometries for carbocation cyclization - how strain associated with bridging affects 5-exo vs. 6-endo selectivity

Journal: Chemical science (Royal Society of Chemistry : 2010)

doi: 10.1039/C3SC51657A

Computed reaction enthalpies (free energies in parentheses; kcal mol −1 ; in DCE) and barriers for [Pt(PH 3 ) 3 ] 2+ and [PdCl 2 (NCMe)] promoted 6- endo and 5- exo cyclizations. Black are for R 1 = R 2 = R 3 = CH 3 ; blue are for R1 = R3 = CH 3 /R2 = H; red are

Figure Legend Snippet: Computed reaction enthalpies (free energies in parentheses; kcal mol −1 ; in DCE) and barriers for [Pt(PH 3 ) 3 ] 2+ and [PdCl 2 (NCMe)] promoted 6- endo and 5- exo cyclizations. Black are for R 1 = R 2 = R 3 = CH 3 ; blue are for R1 = R3 = CH 3 /R2 = H; red are

Techniques Used:

3) Product Images from "Self-Organized Criticality Theory of Autoimmunity"

Article Title: Self-Organized Criticality Theory of Autoimmunity

Journal: PLoS ONE

doi: 10.1371/journal.pone.0008382

Techniques Used: Mouse Assay, Expressing, Injection, Knock-In, Polymerase Chain Reaction, Amplification

4) Product Images from "Palmitoylation Regulates Intracellular Trafficking of ?2 Adrenergic Receptor/Arrestin/Phosphodiesterase 4D Complexes in Cardiomyocytes"

Article Title: Palmitoylation Regulates Intracellular Trafficking of ?2 Adrenergic Receptor/Arrestin/Phosphodiesterase 4D Complexes in Cardiomyocytes

Journal: PLoS ONE

doi: 10.1371/journal.pone.0042658

Mutation of palmitoylation site does not inhibit agonist-induced β 2 AR trafficking. A) β 1 β 2 AR-KO cardiomyocytes expressing wild type or mutant flag-β 2 ARs were stimulated with 10 µM isoproterenol to examine receptor internalization or followed by agonist removal to examine receptor recycling. Receptors were stained by anti-flag antibody M1 and Alex 488-conjugated goat-anti-mouse secondary antibody. Maximal internalization of different flag-β 2 ARs in β 1 β 2 AR-KO cardiomyocytes at 30 minute of stimulation was plotted in bar graph. Receptors remaining on the cell surface after internalization (B and D) or recycling (C) were quantified using Alex 488-conjugated flag antibody labeling. Quantitative measurements of cell surface receptor were average from at least three different experiments. *, p

Figure Legend Snippet: Mutation of palmitoylation site does not inhibit agonist-induced β 2 AR trafficking. A) β 1 β 2 AR-KO cardiomyocytes expressing wild type or mutant flag-β 2 ARs were stimulated with 10 µM isoproterenol to examine receptor internalization or followed by agonist removal to examine receptor recycling. Receptors were stained by anti-flag antibody M1 and Alex 488-conjugated goat-anti-mouse secondary antibody. Maximal internalization of different flag-β 2 ARs in β 1 β 2 AR-KO cardiomyocytes at 30 minute of stimulation was plotted in bar graph. Receptors remaining on the cell surface after internalization (B and D) or recycling (C) were quantified using Alex 488-conjugated flag antibody labeling. Quantitative measurements of cell surface receptor were average from at least three different experiments. *, p

Techniques Used: Mutagenesis, Expressing, Staining, Antibody Labeling, Cell Surface Receptor Assay

Palmitoylation of cysteine 341 of β 2 AR is required for agonist-induced recruitment of β arrestin 2, but not receptor phosphorylation by PKA and GRK. β 1 β 2 AR-KO cardiomyocytes expressing flag-β 2 ARs and β arrestin 2-GFP were serum-starved for 30 minutes before stimulation with 10 µM of isoproterenol as indicated. (A) Cell lysates were incubated with anti-flag M2 beads to immunoprecipitate flag-β 2 ARs. The bound proteins were detected in Western blot by anti-flag and anti-GFP antibodies. *, p

Figure Legend Snippet: Palmitoylation of cysteine 341 of β 2 AR is required for agonist-induced recruitment of β arrestin 2, but not receptor phosphorylation by PKA and GRK. β 1 β 2 AR-KO cardiomyocytes expressing flag-β 2 ARs and β arrestin 2-GFP were serum-starved for 30 minutes before stimulation with 10 µM of isoproterenol as indicated. (A) Cell lysates were incubated with anti-flag M2 beads to immunoprecipitate flag-β 2 ARs. The bound proteins were detected in Western blot by anti-flag and anti-GFP antibodies. *, p

Techniques Used: Expressing, Incubation, Western Blot

$Mutation of palmitoylation site promotes caveolin-dependent β 2 AR internalization upon agonist stimulation. A) Membrane fractionations of β 1 β 2 AR-KO cardiomyocytes expressing β 2 AR or β 2 AR-C341A were blotted with caveolin-3, Src, and flag antibodies. P is the pellet fraction after centrifugation. B) Cell lysates were incubated with M2 beads for immunoprecipitation of flag-β 2 ARs to detect endogenous caveolin-3 association. (C–F) HEK293 cells expressing β 2 AR or together with other proteins were stimulated with 10 µM isoproterenol for 30 minutes to examine receptor internalization. C) Cells expressing flag-β 2 AR alone, D) cells expressing flag-β 2 ARs together dominant negative dynamin K44E, F) cell expressing flag-β 2 ARs were treated with 2 µg/ml filipin for 30 minutes, and E) cells expressing flag-β 2 AR-C341A together with β arrestin 2 (β arr2) or dominant negative β arrestin 2-V54D (β arr2-V54D), before stimulation with isoproterenol 10 µM for 30 minutes to examine receptor internalization. Cells were fixed, and receptors were stained by anti-flag antibody M1 and Alex 594-conjugated goat-anti-mouse secondary antibody.$
Figure Legend Snippet: Mutation of palmitoylation site promotes caveolin-dependent β 2 AR internalization upon agonist stimulation. A) Membrane fractionations of β 1 β 2 AR-KO cardiomyocytes expressing β 2 AR or β 2 AR-C341A were blotted with caveolin-3, Src, and flag antibodies. P is the pellet fraction after centrifugation. B) Cell lysates were incubated with M2 beads for immunoprecipitation of flag-β 2 ARs to detect endogenous caveolin-3 association. (C–F) HEK293 cells expressing β 2 AR or together with other proteins were stimulated with 10 µM isoproterenol for 30 minutes to examine receptor internalization. C) Cells expressing flag-β 2 AR alone, D) cells expressing flag-β 2 ARs together dominant negative dynamin K44E, F) cell expressing flag-β 2 ARs were treated with 2 µg/ml filipin for 30 minutes, and E) cells expressing flag-β 2 AR-C341A together with β arrestin 2 (β arr2) or dominant negative β arrestin 2-V54D (β arr2-V54D), before stimulation with isoproterenol 10 µM for 30 minutes to examine receptor internalization. Cells were fixed, and receptors were stained by anti-flag antibody M1 and Alex 594-conjugated goat-anti-mouse secondary antibody.

Techniques Used: Mutagenesis, Expressing, Centrifugation, Incubation, Immunoprecipitation, Dominant Negative Mutation, Staining

Mutation of palmitoylation reduces β 2 AR/G protein-dependent cAMP production in cardiomyocytes. β 1 β 2 AR-KO cardiomyocytes were infected with adenoviruses expressing β 2 ARs together with the plasma membrane-targeted cAMP FRET biosensor PM-ICUE3 (A and B) or cytoplasmic cAMP FRET biosensor ICUE3 (C and D). Cells were stimulated with 10 nM of isoproterenol (A and C) or 10 µM of isoproterenol (B and D). The maximal increases in FRET ratio were plotted. Rol, rolipram, was added together with Iso at 10 µM. Bar graphs were average from the maximal increases in time courses. *, p

Figure Legend Snippet: Mutation of palmitoylation reduces β 2 AR/G protein-dependent cAMP production in cardiomyocytes. β 1 β 2 AR-KO cardiomyocytes were infected with adenoviruses expressing β 2 ARs together with the plasma membrane-targeted cAMP FRET biosensor PM-ICUE3 (A and B) or cytoplasmic cAMP FRET biosensor ICUE3 (C and D). Cells were stimulated with 10 nM of isoproterenol (A and C) or 10 µM of isoproterenol (B and D). The maximal increases in FRET ratio were plotted. Rol, rolipram, was added together with Iso at 10 µM. Bar graphs were average from the maximal increases in time courses. *, p

Techniques Used: Mutagenesis, Infection, Expressing

Palmitoylation of β 2 AR is required for agonist-dependent recruitment of arrestin-PDE4D complexes to the receptor. β 1 β 2 AR-KO cardiomyocytes expressing flag-β 2 ARs together with GFP-PDE4D5 (A), GFP-PDE4D8 (B), or GFP-PDE4D9 (C) were serum-starved for 30 minutes before stimulation with 10 µM of isoproterenol as indicated. Cell lysates were incubated with anti-flag M2 beads for co-immunoprecipitation of β 2 ARs and PDE4D proteins. The bound proteins were detected in Western blot by anti-flag and anti-GFP antibodies as indicated. D) Flag-β 2 ARs and PDE4D5-RFP were expressed in wild type and arrestins double knockout (KO) MEF cells. Co-IP was done like those in panel (A–C). The bound proteins were detected in Western blot by anti-flag and anti-RFP antibodies as indicated. Western blot was quantified and normalized against the IP protein. Bar graphs were average from 3–5 different experiments. *, p

Figure Legend Snippet: Palmitoylation of β 2 AR is required for agonist-dependent recruitment of arrestin-PDE4D complexes to the receptor. β 1 β 2 AR-KO cardiomyocytes expressing flag-β 2 ARs together with GFP-PDE4D5 (A), GFP-PDE4D8 (B), or GFP-PDE4D9 (C) were serum-starved for 30 minutes before stimulation with 10 µM of isoproterenol as indicated. Cell lysates were incubated with anti-flag M2 beads for co-immunoprecipitation of β 2 ARs and PDE4D proteins. The bound proteins were detected in Western blot by anti-flag and anti-GFP antibodies as indicated. D) Flag-β 2 ARs and PDE4D5-RFP were expressed in wild type and arrestins double knockout (KO) MEF cells. Co-IP was done like those in panel (A–C). The bound proteins were detected in Western blot by anti-flag and anti-RFP antibodies as indicated. Western blot was quantified and normalized against the IP protein. Bar graphs were average from 3–5 different experiments. *, p

Techniques Used: Expressing, Incubation, Immunoprecipitation, Western Blot, Double Knockout, Co-Immunoprecipitation Assay

Stimulation of palmitoylation mutant β 2 AR leads to higher and sustained intracellular cAMP-PKA activities and higher increases in cardiomyocyte contraction rate. β 1 β 2 AR-KO cardiomyocytes expressing β 2 ARs via adenovirus infection were stimulated with isoproterenol (10 µM). The spontaneous contraction rate was recorded before and after isoproterenol stimulation. A) Quantification of isoproterenol-induced cardiomyocyte contraction rate increases over the baseline levels was plotted in the absence or presence of treatment with 2-bromopalmitic acid. B) 20 µg membrane protein containing β 2 ARs was determined by radioligand [ 3 H] DHA binding to measure expression levels of β 2 AR and β 2 AR-C341A. The expressed receptors were also detected by Western blot using anti-flag antibody. β 1 β 2 AR-KO cardiomyocytes expressing β 2 ARs together with cytoplasmic cAMP FRET biosensor ICUE3 (C-E) or PKA activity biosensor AKAR 2.2 (F). (C) and (D) Cells were stimulated with isoproterenol (10 µM) to record the changes of FRET ratio. 2-Bro, 2-bromopalmitic acid (100 µM) was added 15 minutes before Iso stimulation; Quantification of isoproterenol-induced maximal increases in cAMP FRET ratio (E) and PKA FRET ratio (F) was plotted. In each panel, bar graphs were average from the maximal increases in the indicated number of cells (FRET assay) or dishes (beating assay) from at least three independent experiments. *, p

Figure Legend Snippet: Stimulation of palmitoylation mutant β 2 AR leads to higher and sustained intracellular cAMP-PKA activities and higher increases in cardiomyocyte contraction rate. β 1 β 2 AR-KO cardiomyocytes expressing β 2 ARs via adenovirus infection were stimulated with isoproterenol (10 µM). The spontaneous contraction rate was recorded before and after isoproterenol stimulation. A) Quantification of isoproterenol-induced cardiomyocyte contraction rate increases over the baseline levels was plotted in the absence or presence of treatment with 2-bromopalmitic acid. B) 20 µg membrane protein containing β 2 ARs was determined by radioligand [ 3 H] DHA binding to measure expression levels of β 2 AR and β 2 AR-C341A. The expressed receptors were also detected by Western blot using anti-flag antibody. β 1 β 2 AR-KO cardiomyocytes expressing β 2 ARs together with cytoplasmic cAMP FRET biosensor ICUE3 (C-E) or PKA activity biosensor AKAR 2.2 (F). (C) and (D) Cells were stimulated with isoproterenol (10 µM) to record the changes of FRET ratio. 2-Bro, 2-bromopalmitic acid (100 µM) was added 15 minutes before Iso stimulation; Quantification of isoproterenol-induced maximal increases in cAMP FRET ratio (E) and PKA FRET ratio (F) was plotted. In each panel, bar graphs were average from the maximal increases in the indicated number of cells (FRET assay) or dishes (beating assay) from at least three independent experiments. *, p

Techniques Used: Mutagenesis, Expressing, Infection, Binding Assay, Western Blot, Activity Assay

5) Product Images from "Four Phosphates at One Blow: Access to Pentaphosphorylated Magic Spot Nucleotides and Their Analysis by Capillary Electrophoresis"

Article Title: Four Phosphates at One Blow: Access to Pentaphosphorylated Magic Spot Nucleotides and Their Analysis by Capillary Electrophoresis

Journal: The Journal of Organic Chemistry

doi: 10.1021/acs.joc.0c00841

Regioselective Tetraphosphorylation of Nucleosides Using cPyPA ( 6 ) and RNase T2 Synthetic details: (a) 6 (5.0 equiv), ETT (12 equiv), DMF, rt, 45 min; (b) m CPBA (7.5 equiv), 0 °C, 10 min; (c) amine (150 equiv); (d) RNase T2, H 2 O, 37 °C, 3–48 h. (*) In the case of 14 , a mixture of ammonia and diazabicycloundecen (DBU) was applied.

Figure Legend Snippet: Regioselective Tetraphosphorylation of Nucleosides Using cPyPA ( 6 ) and RNase T2 Synthetic details: (a) 6 (5.0 equiv), ETT (12 equiv), DMF, rt, 45 min; (b) m CPBA (7.5 equiv), 0 °C, 10 min; (c) amine (150 equiv); (d) RNase T2, H 2 O, 37 °C, 3–48 h. (*) In the case of 14 , a mixture of ammonia and diazabicycloundecen (DBU) was applied.

Techniques Used:

Fluorescence:

Article Title: The Properties of Microparticles from RAW 264.7 Macrophage Cells Undergoing in vitro Activation or Apoptosis
Article Snippet: .. The concentration of DNA (ng/ml) was calculated according to fluorescence values for a standard solution of double-stranded DNA from calf thymus (Worthington Biochemical Corporation, Lakewood, NJ). .. Briefly, actively growing RAW 264.7 cells were plated at a density of 2-3 × 106 cells per T175 tissue culture flask in complete medium and incubated overnight at 37°C, 5% CO2 .

Isolation:

Article Title: Characterization of Valvular Interstitial Cell Function in Three Dimensional Matrix Metalloproteinase Degradable PEG Hydrogels
Article Snippet: .. Double stranded DNA (dsDNA) was isolated from similarly sized hydrogel constructs by papainase (Worthington) digestion at 60°C overnight. .. The resulting digestion solution was analyzed for dsDNA content using the Quant-It PicoGreen dsDNA assay (Invitrogen) per manufacturer's instructions.

Construct:

Enzyme-linked Immunosorbent Assay:

Article Title: The Inhibition of Anti-DNA Binding to DNA by Nucleic Acid Binding Polymers
Article Snippet: .. For the direct binding assay, wells of ELISA plates were coated with 5 µg/ml native double stranded calf thymus (CT) DNA (Worthington Biochemical, Lakewood, NJ) in 1 x SSC (150 mM NaCl, 15 mM Na citrate, pH 7) overnight at 4°C. .. For assays involving biotinylated DNA, native CT DNA was purified by phenol:chloroform extraction and then diluted to 0.5 mg/ml in 10 mM Tris, 1 mM EDTA buffer pH 8.0 (TE buffer).

Concentration Assay:

Article Title: Solution Structure of the B3 DNA Binding Domain of the Arabidopsis Cold-Responsive Transcription Factor RAV1 W⃞
Article Snippet: .. The concentration of the double-stranded DNA was determined using an extinction coefficient calculated after digestion of the strands with phosphodiesterase I (Worthington Biochemical, Lakewood, NJ). .. The HOMOLOGY module of Insight II (Accelrys, San Diego, CA) was used for the structural modeling of the ARF1- and ABI3-B3 domains based on the sequence homology to RAV1-B3.

Article Title: Structural Basis for Sequence-specific DNA Recognition by an Arabidopsis WRKY Transcription Factor *
Article Snippet: .. The concentration of the protein was determined at A 280 with an extinction coefficient estimated from the amino acid sequence , whereas that of the double-stranded DNA was determined at A 260 using an extinction coefficient that was calculated after digestion of the strands with phosphodiesterase I (Worthington). .. For the NMR measurements, 0.4–1.0 m m 1:1 protein-DNA complex was dissolved in 20 m m potassium phosphate buffer (pH 6.0) containing 200 m m KCl, 20 μ m ZnCl2 , 1 m m deuterated dithiothreitol (Isotec Inc.), 0.05 m m sodium 2,2-dimethyl-2-silapentane-5-sulfonate, and 5% D2 O unless stated otherwise.

other:

Article Title: The Mycoplasma pneumoniae MPN229 gene encodes a protein that selectively binds single-stranded DNA and stimulates Recombinase A-mediated DNA strand exchange
Article Snippet: The DNA-cellulose was poured into a column, and protein was eluted stepwise by the addition of 0.5 ml of a buffer (20 mM Tris-HCl pH 7.5, 1 mM DTT) containing increasing concentrations of NaCl (from 200 to 1500 mM).

dsdna (Worthington Biochemical)

Structured Review

Related Products / Commonly Used Together

Images

1) Product Images from "Self-Organized Criticality Theory of Autoimmunity"

2) Product Images from "Carbonium vs. carbenium ion-like transition state geometries for carbocation cyclization - how strain associated with bridging affects 5-exo vs. 6-endo selectivity"

3) Product Images from "Self-Organized Criticality Theory of Autoimmunity"

4) Product Images from "Palmitoylation Regulates Intracellular Trafficking of ?2 Adrenergic Receptor/Arrestin/Phosphodiesterase 4D Complexes in Cardiomyocytes"

5) Product Images from "Four Phosphates at One Blow: Access to Pentaphosphorylated Magic Spot Nucleotides and Their Analysis by Capillary Electrophoresis"

Related Articles

Fluorescence:

Isolation:

Construct:

Enzyme-linked Immunosorbent Assay:

Concentration Assay:

other:

Sequencing:

Binding Assay:

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