aqp3  (Alomone Labs)


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    Name:
    Anti Aquaporin 3 Antibody
    Description:
    Anti Aquaporin 3 Antibody AQP 003 is a highly specific antibody directed against an epitope of the rat protein The antibody can be used in western blot immunoprecipitation indirect flow cytometry and immunohistochemistry applications It has been designed to recognize the AQP3 channel from rat human and mouse samples
    Catalog Number:
    AQP-003
    Price:
    495.0
    Category:
    Primary Antibody
    Applications:
    Indirect Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
    Purity:
    Affinity purified on immobilized antigen.
    Immunogen:
    Synthetic peptide
    Size:
    50 mcl
    Antibody Type:
    Polyclonal Primary Antibodies
    Format:
    Lyophilized Powder
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
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    Structured Review

    Alomone Labs aqp3
    Anti Aquaporin 3 Antibody
    Anti Aquaporin 3 Antibody AQP 003 is a highly specific antibody directed against an epitope of the rat protein The antibody can be used in western blot immunoprecipitation indirect flow cytometry and immunohistochemistry applications It has been designed to recognize the AQP3 channel from rat human and mouse samples
    https://www.bioz.com/result/aqp3/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    aqp3 - by Bioz Stars, 2021-09
    94/100 stars

    Images

    Related Articles

    Blocking Assay:

    Article Title: AQP3 Increases Intercellular Cohesion in NSCLC A549 Cell Spheroids through Exploratory Cell Protrusions
    Article Snippet: .. In short, the cells on coverslips were fixed with 4% paraformaldehyde for 10 min and permeabilized with 0.15% Triton-X 100 for 5 min. Then, the cells were blocked for 1 h with the blocking solution of 3% bovine serum albumin in PBS and incubated with the primary antibody against AQP3 for 2 h at room temperature. ..

    Article Title: Differential roles of VPS and RAAS in water homeostasis and a risk for kidney dysfunction in rats undergoing rapid fasting/dehydration with regular exercise
    Article Snippet: .. After blocking with a blocking solution (AQPs, 5% skimmed milk; caspase‐3, Blocking One [Nacalai Tesque]), the membranes were incubated overnight with the rabbit primary antibody anti‐AQP2 (1:5,000 dilution) (Alomone Labs, Jerusalem, Israel), anti‐AQP3 (1:10,000 dilution) (Alomone Labs), anti‐AQP4 (1:10,000 dilution) (Alomone Labs), or anti‐caspase‐3 (1:2000) (Cell Signaling Technology, Beverly, MA, USA) at 4°C with gentle shaking. ..

    Incubation:

    Article Title: AQP3 Increases Intercellular Cohesion in NSCLC A549 Cell Spheroids through Exploratory Cell Protrusions
    Article Snippet: .. In short, the cells on coverslips were fixed with 4% paraformaldehyde for 10 min and permeabilized with 0.15% Triton-X 100 for 5 min. Then, the cells were blocked for 1 h with the blocking solution of 3% bovine serum albumin in PBS and incubated with the primary antibody against AQP3 for 2 h at room temperature. ..

    Article Title: Differential roles of VPS and RAAS in water homeostasis and a risk for kidney dysfunction in rats undergoing rapid fasting/dehydration with regular exercise
    Article Snippet: .. After blocking with a blocking solution (AQPs, 5% skimmed milk; caspase‐3, Blocking One [Nacalai Tesque]), the membranes were incubated overnight with the rabbit primary antibody anti‐AQP2 (1:5,000 dilution) (Alomone Labs, Jerusalem, Israel), anti‐AQP3 (1:10,000 dilution) (Alomone Labs), anti‐AQP4 (1:10,000 dilution) (Alomone Labs), or anti‐caspase‐3 (1:2000) (Cell Signaling Technology, Beverly, MA, USA) at 4°C with gentle shaking. ..

    Immunofluorescence:

    Article Title: Aquaporin-3 regulates endosome-to-cytosol transfer via lipid peroxidation for cross presentation
    Article Snippet: .. Immunofluorescence and phagosome association analysis On day 6 of culture, WT and AQP3 -/- BMDCs were fixed in 4% PFA and permeabilized with 0.05% saponin, followed by detection with anti-AQP3 (Alomone Labs) and Hoechst 33342 (Life Technologies). ..

    Article Title: Aquaporin-3 regulates endosome-to-cytosol transfer via lipid peroxidation for cross presentation
    Article Snippet: .. Immunofluorescence and phagosome association analysis On day 6 of culture, WT and AQP3 -/- BMDCs were fixed in 4% PFA and permeabilized with 0.05% saponin, followed by detection with anti-AQP3 (Alomone Labs) and Hoechst 33342 (Life Technologies). ..

    Western Blot:

    Article Title: Dioleoylphosphatidylglycerol Accelerates Corneal Epithelial Wound Healing
    Article Snippet: .. The antibody recognizing AQP3 (Alomone Labs, Jerusalem, Israel) was previously validated in our laboratory using western analysis and by demonstrating increased AQP3 immunoreactivity in the skin of an epidermal-targeted AQP3-overexpressing transgenic mouse model. , . ..

    Article Title: Dioleoylphosphatidylglycerol Accelerates Corneal Epithelial Wound Healing
    Article Snippet: .. Co-ImmunoprecipitationCells were harvested using radioimmunoprecipitation assay (RIPA) buffer and immunoprecipitated using an antibody recognizing AQP3 (Alomone Labs) as described in Zheng et al. Immunoprecipitates were collected, solubilized in Laemmli buffer and 30 µg protein (determined using a BioRad protein assay kit with bovine serum albumin as the standard), and analyzed by western blotting, as described below. ..

    Transgenic Assay:

    Article Title: Dioleoylphosphatidylglycerol Accelerates Corneal Epithelial Wound Healing
    Article Snippet: .. The antibody recognizing AQP3 (Alomone Labs, Jerusalem, Israel) was previously validated in our laboratory using western analysis and by demonstrating increased AQP3 immunoreactivity in the skin of an epidermal-targeted AQP3-overexpressing transgenic mouse model. , . ..

    Radio Immunoprecipitation:

    Article Title: Dioleoylphosphatidylglycerol Accelerates Corneal Epithelial Wound Healing
    Article Snippet: .. Co-ImmunoprecipitationCells were harvested using radioimmunoprecipitation assay (RIPA) buffer and immunoprecipitated using an antibody recognizing AQP3 (Alomone Labs) as described in Zheng et al. Immunoprecipitates were collected, solubilized in Laemmli buffer and 30 µg protein (determined using a BioRad protein assay kit with bovine serum albumin as the standard), and analyzed by western blotting, as described below. ..

    Immunoprecipitation:

    Article Title: Dioleoylphosphatidylglycerol Accelerates Corneal Epithelial Wound Healing
    Article Snippet: .. Co-ImmunoprecipitationCells were harvested using radioimmunoprecipitation assay (RIPA) buffer and immunoprecipitated using an antibody recognizing AQP3 (Alomone Labs) as described in Zheng et al. Immunoprecipitates were collected, solubilized in Laemmli buffer and 30 µg protein (determined using a BioRad protein assay kit with bovine serum albumin as the standard), and analyzed by western blotting, as described below. ..

    Immunostaining:

    Article Title: Pax2 and Pax8 Proteins Regulate Urea Transporters and Aquaporins to Control Urine Concentration in the Adult Kidney
    Article Snippet: .. Antibodies used for immunostaining were Pax8, 1:300 (10336–1-AP; Proteintech, Rosemont IL); SLC14a2, 1:50 (ab95365; Abcam); AQP1, 1:100 (AQP-001; Alomone Labs, Jerusalem, Israel); AQP2, 1:100 (AQP-002; Alomone Labs); AQP3, 1:100 (AQP-003; Alomone Labs); and AQP4, 1:100 (AQP-004; Alomone Labs). ..

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  • 94
    Alomone Labs anti aqp3
    <t>AQP3</t> Immunoreactivity was Mislocalized in a Majority of Psoriatic Lesions (A through C) Archived paraffin-embedded psoriasis samples were sectioned (4 μm) and deparaffinized. Sections were then stained with an antibody recognizing AQP3 and visualized with an ABC kit and DAB. Results from three patients are shown and the staining pattern is representative of 8 of 10 psoriatic samples examined. (D) A negative control was performed by omitting primary antibody. Note that AQP3 is localized to the cytoplasm rather than to the plasma membrane in a majority of the psoriatic epidermis. All sections were counterstained with hematoxylin.
    Anti Aqp3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti aqp3/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti aqp3 - by Bioz Stars, 2021-09
    94/100 stars
      Buy from Supplier

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    AQP3 Immunoreactivity was Mislocalized in a Majority of Psoriatic Lesions (A through C) Archived paraffin-embedded psoriasis samples were sectioned (4 μm) and deparaffinized. Sections were then stained with an antibody recognizing AQP3 and visualized with an ABC kit and DAB. Results from three patients are shown and the staining pattern is representative of 8 of 10 psoriatic samples examined. (D) A negative control was performed by omitting primary antibody. Note that AQP3 is localized to the cytoplasm rather than to the plasma membrane in a majority of the psoriatic epidermis. All sections were counterstained with hematoxylin.

    Journal: Archives of dermatological research

    Article Title: ABNORMAL AQUAPORIN-3 PROTEIN EXPRESSION IN HYPERPROLIFERATIVE SKIN DISORDERS

    doi: 10.1007/s00403-011-1136-x

    Figure Lengend Snippet: AQP3 Immunoreactivity was Mislocalized in a Majority of Psoriatic Lesions (A through C) Archived paraffin-embedded psoriasis samples were sectioned (4 μm) and deparaffinized. Sections were then stained with an antibody recognizing AQP3 and visualized with an ABC kit and DAB. Results from three patients are shown and the staining pattern is representative of 8 of 10 psoriatic samples examined. (D) A negative control was performed by omitting primary antibody. Note that AQP3 is localized to the cytoplasm rather than to the plasma membrane in a majority of the psoriatic epidermis. All sections were counterstained with hematoxylin.

    Article Snippet: Slides for AQP3 staining were deparaffinized, washed twice for 5 minutes in phosphate-buffered saline (PBS), incubated 30 minutes in 3% hydrogen peroxide, washed twice for 5 minutes in PBS, incubated 1 hour in 0.3% goat serum, and then incubated overnight with anti-AQP3 (Alomone Labs, Jerusalem, Israel; 1:1000 dilution) in a humidified chamber at 4°C.

    Techniques: Staining, Negative Control

    AQP3 Immunoreactivity was “Patchy” in Squamous Cell Carcinoma (SCC) Archived paraffin-embedded squamous cell carcinoma (SCC) samples were sectioned (4 μm) and deparaffinized. Sections were then stained with antibodies recognizing AQP3 and visualized with an ABC kit and DAB. Sections were counterstained with hematoxylin. Illustrated in panels A through C is AQP3 staining in 3 patients with results representative of 5 of 5 SCCs. A negative control in which the primary antibody was omitted showed no AQP3 staining (panel D).

    Journal: Archives of dermatological research

    Article Title: ABNORMAL AQUAPORIN-3 PROTEIN EXPRESSION IN HYPERPROLIFERATIVE SKIN DISORDERS

    doi: 10.1007/s00403-011-1136-x

    Figure Lengend Snippet: AQP3 Immunoreactivity was “Patchy” in Squamous Cell Carcinoma (SCC) Archived paraffin-embedded squamous cell carcinoma (SCC) samples were sectioned (4 μm) and deparaffinized. Sections were then stained with antibodies recognizing AQP3 and visualized with an ABC kit and DAB. Sections were counterstained with hematoxylin. Illustrated in panels A through C is AQP3 staining in 3 patients with results representative of 5 of 5 SCCs. A negative control in which the primary antibody was omitted showed no AQP3 staining (panel D).

    Article Snippet: Slides for AQP3 staining were deparaffinized, washed twice for 5 minutes in phosphate-buffered saline (PBS), incubated 30 minutes in 3% hydrogen peroxide, washed twice for 5 minutes in PBS, incubated 1 hour in 0.3% goat serum, and then incubated overnight with anti-AQP3 (Alomone Labs, Jerusalem, Israel; 1:1000 dilution) in a humidified chamber at 4°C.

    Techniques: Staining, Negative Control

    AQP3 Staining was Reduced in Ki67-Positive Cells in Squamous Cell Carcinoma Archived paraffin-embedded squamous cell carcinoma (SCC) samples were sectioned (4 μm) and deparaffinized. Sequential serial sections were then stained with antibodies recognizing (A, C) AQP3 or (B, D) Ki67 [an antigen expressed in proliferating cells (Dako, Carpinteria, CA)] and visualized with an ABC kit and DAB. Sections were counterstained with hematoxylin. Note that cells that are Ki67 positive exhibit reduced or absent AQP3 staining (arrows in panels C and D). The inverse correlation of Ki67 positivity with AQP3 immunoreactivity is representative of 4 of 4 SCCs. (E) Human tonsil stained with Ki67 as a positive control. (F) Primary antibody was omitted as a negative control.

    Journal: Archives of dermatological research

    Article Title: ABNORMAL AQUAPORIN-3 PROTEIN EXPRESSION IN HYPERPROLIFERATIVE SKIN DISORDERS

    doi: 10.1007/s00403-011-1136-x

    Figure Lengend Snippet: AQP3 Staining was Reduced in Ki67-Positive Cells in Squamous Cell Carcinoma Archived paraffin-embedded squamous cell carcinoma (SCC) samples were sectioned (4 μm) and deparaffinized. Sequential serial sections were then stained with antibodies recognizing (A, C) AQP3 or (B, D) Ki67 [an antigen expressed in proliferating cells (Dako, Carpinteria, CA)] and visualized with an ABC kit and DAB. Sections were counterstained with hematoxylin. Note that cells that are Ki67 positive exhibit reduced or absent AQP3 staining (arrows in panels C and D). The inverse correlation of Ki67 positivity with AQP3 immunoreactivity is representative of 4 of 4 SCCs. (E) Human tonsil stained with Ki67 as a positive control. (F) Primary antibody was omitted as a negative control.

    Article Snippet: Slides for AQP3 staining were deparaffinized, washed twice for 5 minutes in phosphate-buffered saline (PBS), incubated 30 minutes in 3% hydrogen peroxide, washed twice for 5 minutes in PBS, incubated 1 hour in 0.3% goat serum, and then incubated overnight with anti-AQP3 (Alomone Labs, Jerusalem, Israel; 1:1000 dilution) in a humidified chamber at 4°C.

    Techniques: Staining, Positive Control, Negative Control

    AQP3 Immunoreactivity was Reduced or Absent in Basal Cell Carcinoma (BCC) Archived paraffin-embedded basal cell carcinoma samples were sectioned (4 μm), deparaffinized, stained with antibodies recognizing AQP3 and visualized with an ABC kit and 3,3’-diaminobenzidine (DAB). Sections were counterstained with hematoxylin. Results illustrate three BCCs from different patients and are representative of 13 of 13 BCCs. The results were quantified as 3+ (intense staining), 2+ (moderate staining), + (weak staining) or 0 (no staining) by three independent observers and the values averaged. Shown in panel D is the quantitation (means ± SEM) of the 13 BCCs relative to normal-appearing overlying epidermis with the data analyzed using a student's t-test; *p

    Journal: Archives of dermatological research

    Article Title: ABNORMAL AQUAPORIN-3 PROTEIN EXPRESSION IN HYPERPROLIFERATIVE SKIN DISORDERS

    doi: 10.1007/s00403-011-1136-x

    Figure Lengend Snippet: AQP3 Immunoreactivity was Reduced or Absent in Basal Cell Carcinoma (BCC) Archived paraffin-embedded basal cell carcinoma samples were sectioned (4 μm), deparaffinized, stained with antibodies recognizing AQP3 and visualized with an ABC kit and 3,3’-diaminobenzidine (DAB). Sections were counterstained with hematoxylin. Results illustrate three BCCs from different patients and are representative of 13 of 13 BCCs. The results were quantified as 3+ (intense staining), 2+ (moderate staining), + (weak staining) or 0 (no staining) by three independent observers and the values averaged. Shown in panel D is the quantitation (means ± SEM) of the 13 BCCs relative to normal-appearing overlying epidermis with the data analyzed using a student's t-test; *p

    Article Snippet: Slides for AQP3 staining were deparaffinized, washed twice for 5 minutes in phosphate-buffered saline (PBS), incubated 30 minutes in 3% hydrogen peroxide, washed twice for 5 minutes in PBS, incubated 1 hour in 0.3% goat serum, and then incubated overnight with anti-AQP3 (Alomone Labs, Jerusalem, Israel; 1:1000 dilution) in a humidified chamber at 4°C.

    Techniques: Staining, Quantitation Assay

    AQP3 and PLD2 co-localize in the cornea in situ and in immortalized human corneal limbal epithelial cells in vitro. Formalin-fixed human corneas obtained from the Georgia Eye Bank were paraffin-embedded and sectioned onto microscope slides. Sections were stained using an Opal immunofluorescence kit and incubated with a rabbit polyclonal antibody recognizing PLD2 and a mouse monoclonal antibody against AQP3. Sections were then processed according to the supplier's instructions with red staining (Opal 570) representing PLD2 and green (Opal 520) representing AQP3. Staining was visualized using confocal microscopy on a Zeiss laser-scanning microscope. ( A ) A more central region of the corneal epithelium, ( B ) the limbal region of the cornea, and ( C ) immortalized human corneal limbal epithelial cells are shown. Results are representative of at least three corneas or cell passages; negative controls in which primary antibodies were omitted demonstrated essentially no staining.

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Dioleoylphosphatidylglycerol Accelerates Corneal Epithelial Wound Healing

    doi: 10.1167/iovs.61.3.29

    Figure Lengend Snippet: AQP3 and PLD2 co-localize in the cornea in situ and in immortalized human corneal limbal epithelial cells in vitro. Formalin-fixed human corneas obtained from the Georgia Eye Bank were paraffin-embedded and sectioned onto microscope slides. Sections were stained using an Opal immunofluorescence kit and incubated with a rabbit polyclonal antibody recognizing PLD2 and a mouse monoclonal antibody against AQP3. Sections were then processed according to the supplier's instructions with red staining (Opal 570) representing PLD2 and green (Opal 520) representing AQP3. Staining was visualized using confocal microscopy on a Zeiss laser-scanning microscope. ( A ) A more central region of the corneal epithelium, ( B ) the limbal region of the cornea, and ( C ) immortalized human corneal limbal epithelial cells are shown. Results are representative of at least three corneas or cell passages; negative controls in which primary antibodies were omitted demonstrated essentially no staining.

    Article Snippet: The antibody recognizing AQP3 (Alomone Labs, Jerusalem, Israel) was previously validated in our laboratory using western analysis and by demonstrating increased AQP3 immunoreactivity in the skin of an epidermal-targeted AQP3-overexpressing transgenic mouse model. , .

    Techniques: In Situ, In Vitro, Microscopy, Staining, Immunofluorescence, Incubation, Confocal Microscopy, Laser-Scanning Microscopy

    The ability of DOPG to enhance corneal epithelial wound healing is not affected by the sex of the animal. Male and female AQP3 knockout mice treated as in Figure 6 were analyzed separately (n = 4–6). Regression slopes (the healing rate) were compared by an unpaired Student's t -test and illustrated as values for individual animals by sex as indicated ( * P

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Dioleoylphosphatidylglycerol Accelerates Corneal Epithelial Wound Healing

    doi: 10.1167/iovs.61.3.29

    Figure Lengend Snippet: The ability of DOPG to enhance corneal epithelial wound healing is not affected by the sex of the animal. Male and female AQP3 knockout mice treated as in Figure 6 were analyzed separately (n = 4–6). Regression slopes (the healing rate) were compared by an unpaired Student's t -test and illustrated as values for individual animals by sex as indicated ( * P

    Article Snippet: The antibody recognizing AQP3 (Alomone Labs, Jerusalem, Israel) was previously validated in our laboratory using western analysis and by demonstrating increased AQP3 immunoreactivity in the skin of an epidermal-targeted AQP3-overexpressing transgenic mouse model. , .

    Techniques: Knock-Out, Mouse Assay

    Immortalized human corneal epithelial cells express both AQP3 and PLD2 proteins, which can be co-immunoprecipitated from these cells. SV40-immortalized human corneal epithelial cells were cultured in a 1:1 mixture of defined keratinocyte serum-free (DKSF) medium and Minimum Essential Medium until approximately 70% confluent. The cells were harvested using RIPA buffer and immunoprecipitated using antibody recognizing AQP3 as described in the Materials and Methods section. Immunoprecipitates were collected and analyzed by western blotting with antibodies recognizing AQP3 or PLD2 as indicated. Molecular weight standards were separated in the far right lane and appear as light bands. Results are representative of at least three separate experiments.

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Dioleoylphosphatidylglycerol Accelerates Corneal Epithelial Wound Healing

    doi: 10.1167/iovs.61.3.29

    Figure Lengend Snippet: Immortalized human corneal epithelial cells express both AQP3 and PLD2 proteins, which can be co-immunoprecipitated from these cells. SV40-immortalized human corneal epithelial cells were cultured in a 1:1 mixture of defined keratinocyte serum-free (DKSF) medium and Minimum Essential Medium until approximately 70% confluent. The cells were harvested using RIPA buffer and immunoprecipitated using antibody recognizing AQP3 as described in the Materials and Methods section. Immunoprecipitates were collected and analyzed by western blotting with antibodies recognizing AQP3 or PLD2 as indicated. Molecular weight standards were separated in the far right lane and appear as light bands. Results are representative of at least three separate experiments.

    Article Snippet: The antibody recognizing AQP3 (Alomone Labs, Jerusalem, Israel) was previously validated in our laboratory using western analysis and by demonstrating increased AQP3 immunoreactivity in the skin of an epidermal-targeted AQP3-overexpressing transgenic mouse model. , .

    Techniques: Immunoprecipitation, Cell Culture, Western Blot, Molecular Weight

    The interaction between PLD2 and AQP3 is likely direct. Sf9 insect cells were infected with baculovirus expressing either His-tagged PLD2 (PLD2-His; Fig. 4 A, left panel) or GST-tagged AQP3 (AQP3-GST; Fig. 4 B) alone or with both AQP3-GST and PLD2-His ( Fig. 4 A, right panel, Fig. 4 B). Equal volumes of pre-cleared Sf9 lysates were then immunoprecipitated (IP) with anti-GST antibody (to pull down AQP3-GST) or anti-PLD2 antibody, and the immunoprecipitates were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting (IB) using antibodies recognizing GST (for AQP3-GST) or His (for PLD2-His) as indicated. A 1/10 volume of lysate was similarly analyzed (Input). Results are representative of three experiments.

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Dioleoylphosphatidylglycerol Accelerates Corneal Epithelial Wound Healing

    doi: 10.1167/iovs.61.3.29

    Figure Lengend Snippet: The interaction between PLD2 and AQP3 is likely direct. Sf9 insect cells were infected with baculovirus expressing either His-tagged PLD2 (PLD2-His; Fig. 4 A, left panel) or GST-tagged AQP3 (AQP3-GST; Fig. 4 B) alone or with both AQP3-GST and PLD2-His ( Fig. 4 A, right panel, Fig. 4 B). Equal volumes of pre-cleared Sf9 lysates were then immunoprecipitated (IP) with anti-GST antibody (to pull down AQP3-GST) or anti-PLD2 antibody, and the immunoprecipitates were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting (IB) using antibodies recognizing GST (for AQP3-GST) or His (for PLD2-His) as indicated. A 1/10 volume of lysate was similarly analyzed (Input). Results are representative of three experiments.

    Article Snippet: The antibody recognizing AQP3 (Alomone Labs, Jerusalem, Israel) was previously validated in our laboratory using western analysis and by demonstrating increased AQP3 immunoreactivity in the skin of an epidermal-targeted AQP3-overexpressing transgenic mouse model. , .

    Techniques: Infection, Expressing, Immunoprecipitation, Polyacrylamide Gel Electrophoresis, SDS Page

    Human corneal epithelial cells express AQP3 in situ. Formalin-fixed human corneas obtained from the Georgia Eye Bank were paraffin-embedded and sectioned. After antigen retrieval, sections were incubated with an antibody recognizing AQP3 and visualized with an ABC staining kit using 3,3′-diaminobenzidine as chromogen (brown staining), as described in the Materials and Methods section. Sections were counterstained with hematoxylin (blue staining) and photographed. Results are representative of the staining of random sections of right and left corneas from at least three individuals. ( A , B ) AQP3 staining observed in two different corneas. ( C ) A negative control performed by omission of the primary antibody.

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Dioleoylphosphatidylglycerol Accelerates Corneal Epithelial Wound Healing

    doi: 10.1167/iovs.61.3.29

    Figure Lengend Snippet: Human corneal epithelial cells express AQP3 in situ. Formalin-fixed human corneas obtained from the Georgia Eye Bank were paraffin-embedded and sectioned. After antigen retrieval, sections were incubated with an antibody recognizing AQP3 and visualized with an ABC staining kit using 3,3′-diaminobenzidine as chromogen (brown staining), as described in the Materials and Methods section. Sections were counterstained with hematoxylin (blue staining) and photographed. Results are representative of the staining of random sections of right and left corneas from at least three individuals. ( A , B ) AQP3 staining observed in two different corneas. ( C ) A negative control performed by omission of the primary antibody.

    Article Snippet: The antibody recognizing AQP3 (Alomone Labs, Jerusalem, Israel) was previously validated in our laboratory using western analysis and by demonstrating increased AQP3 immunoreactivity in the skin of an epidermal-targeted AQP3-overexpressing transgenic mouse model. , .

    Techniques: In Situ, Incubation, Staining, Negative Control

    DOPG accelerates corneal epithelial wound healing in AQP3 knockout mice. Anesthetized mice (n = 10–12) received corneal wounds produced by scraping the epithelium with an Algerbrush. Fluorescein was added to the wounded eye, and the wound was photographed. Saline control vehicle (Con) or DOPG in saline (100 µg/mL) was then applied to the eye. This process was repeated every 4 hours for 28 hours. The area of the photographed wounds was digitized and expressed as a percentage of the initial wound area. ( A ) Mean ± SEM of the wound area at each Con versus DOPG time point; these values were compared using an unpaired Student's t-test, with ** P

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Dioleoylphosphatidylglycerol Accelerates Corneal Epithelial Wound Healing

    doi: 10.1167/iovs.61.3.29

    Figure Lengend Snippet: DOPG accelerates corneal epithelial wound healing in AQP3 knockout mice. Anesthetized mice (n = 10–12) received corneal wounds produced by scraping the epithelium with an Algerbrush. Fluorescein was added to the wounded eye, and the wound was photographed. Saline control vehicle (Con) or DOPG in saline (100 µg/mL) was then applied to the eye. This process was repeated every 4 hours for 28 hours. The area of the photographed wounds was digitized and expressed as a percentage of the initial wound area. ( A ) Mean ± SEM of the wound area at each Con versus DOPG time point; these values were compared using an unpaired Student's t-test, with ** P

    Article Snippet: The antibody recognizing AQP3 (Alomone Labs, Jerusalem, Israel) was previously validated in our laboratory using western analysis and by demonstrating increased AQP3 immunoreactivity in the skin of an epidermal-targeted AQP3-overexpressing transgenic mouse model. , .

    Techniques: Knock-Out, Mouse Assay, Produced

    Effects of the rapid restriction of food and water intake on the renal expression levels of water channels in rats with and without regular exercise. (a) Gene expression levels of AQP2 in the kidney medulla. The mRNA levels of AQP2 were obtained by quantitative RT‐PCR and normalized to those of GAPDH. Expression levels are displayed relative to those of the control group receiving neither regular exercise nor rapid restriction (1.0). (b) Protein levels of AQP2 in the kidney medulla. The amounts of the nonglycosylated (29 kDa) and glycosylated form (35 kDa) forms of AQP2 protein were analyzed by Western blot (left panel) and densitometry, and the sum of the two values was compared between the groups (right panel). The image shows representative immunoblots. (c–f) Gene expression levels and protein levels of AQP3 (c and d, respectively) and AQP4 (e and f, respectively) in the kidney medulla. Data are obtained and displayed as described in (a and b). Data are expressed as mean ± SEM (mRNA levels, n = 5/group; protein levels, n = 4/group). * p

    Journal: Physiological Reports

    Article Title: Differential roles of VPS and RAAS in water homeostasis and a risk for kidney dysfunction in rats undergoing rapid fasting/dehydration with regular exercise

    doi: 10.14814/phy2.14670

    Figure Lengend Snippet: Effects of the rapid restriction of food and water intake on the renal expression levels of water channels in rats with and without regular exercise. (a) Gene expression levels of AQP2 in the kidney medulla. The mRNA levels of AQP2 were obtained by quantitative RT‐PCR and normalized to those of GAPDH. Expression levels are displayed relative to those of the control group receiving neither regular exercise nor rapid restriction (1.0). (b) Protein levels of AQP2 in the kidney medulla. The amounts of the nonglycosylated (29 kDa) and glycosylated form (35 kDa) forms of AQP2 protein were analyzed by Western blot (left panel) and densitometry, and the sum of the two values was compared between the groups (right panel). The image shows representative immunoblots. (c–f) Gene expression levels and protein levels of AQP3 (c and d, respectively) and AQP4 (e and f, respectively) in the kidney medulla. Data are obtained and displayed as described in (a and b). Data are expressed as mean ± SEM (mRNA levels, n = 5/group; protein levels, n = 4/group). * p

    Article Snippet: After blocking with a blocking solution (AQPs, 5% skimmed milk; caspase‐3, Blocking One [Nacalai Tesque]), the membranes were incubated overnight with the rabbit primary antibody anti‐AQP2 (1:5,000 dilution) (Alomone Labs, Jerusalem, Israel), anti‐AQP3 (1:10,000 dilution) (Alomone Labs), anti‐AQP4 (1:10,000 dilution) (Alomone Labs), or anti‐caspase‐3 (1:2000) (Cell Signaling Technology, Beverly, MA, USA) at 4°C with gentle shaking.

    Techniques: Expressing, Quantitative RT-PCR, Western Blot

    A549 cell aggregation is an active process. ( A ) The effect of ROCK on A549 cell aggregation. The phase-contrast micrographs show the morphologies of the A549 cells following treatment with a ROCK inhibitor, Y-27632, and the Rho activator Ⅱ. ( B ) The effects of AQP3 knockdown with siRNA on actomyosin cytoskeleton remodeling. A549 cells were stained with anti-AQP3 antibody, followed by Fluorescein-conjugated antibody (green). The actin microfilaments were stained with rhodamine-conjugated phalloidin (red), and the nuclei were stained with DAPI (blue). ( C ) The effects of AQP3 knockdown with siRNA on the apoptosis signaling pathway. Twenty-four h following siRNA transfection in 2D culture condition, the cells were further incubated in the 2D or 3D culture condition. Then, cells were harvested to confirm the effect of AQP3 knockdown on the apoptotic molecular signatures using Western blotting. α-tubulin was used as an internal control.

    Journal: International Journal of Molecular Sciences

    Article Title: AQP3 Increases Intercellular Cohesion in NSCLC A549 Cell Spheroids through Exploratory Cell Protrusions

    doi: 10.3390/ijms22084287

    Figure Lengend Snippet: A549 cell aggregation is an active process. ( A ) The effect of ROCK on A549 cell aggregation. The phase-contrast micrographs show the morphologies of the A549 cells following treatment with a ROCK inhibitor, Y-27632, and the Rho activator Ⅱ. ( B ) The effects of AQP3 knockdown with siRNA on actomyosin cytoskeleton remodeling. A549 cells were stained with anti-AQP3 antibody, followed by Fluorescein-conjugated antibody (green). The actin microfilaments were stained with rhodamine-conjugated phalloidin (red), and the nuclei were stained with DAPI (blue). ( C ) The effects of AQP3 knockdown with siRNA on the apoptosis signaling pathway. Twenty-four h following siRNA transfection in 2D culture condition, the cells were further incubated in the 2D or 3D culture condition. Then, cells were harvested to confirm the effect of AQP3 knockdown on the apoptotic molecular signatures using Western blotting. α-tubulin was used as an internal control.

    Article Snippet: In short, the cells on coverslips were fixed with 4% paraformaldehyde for 10 min and permeabilized with 0.15% Triton-X 100 for 5 min. Then, the cells were blocked for 1 h with the blocking solution of 3% bovine serum albumin in PBS and incubated with the primary antibody against AQP3 for 2 h at room temperature.

    Techniques: Staining, Transfection, Incubation, Western Blot

    The effect of AQP3 on spatiotemporal dynamics of protrusions. ( A ) Polymerase chain reaction and ( B ) Quantitative real-time reverse transcription-polymerase chain reaction of the transcript levels of the organic hydroxyl transport genes. The data shown here represent three independent experiments, and the values represent the mean ± SEM of triplicate samples. The level of each mRNA was normalized to that of the GAPDH mRNA in the same sample and presented as the fold-change over that of the 2D culture control cells. The differences in expression levels were evaluated for significance using two-tailed t-tests with unequal variance. * p

    Journal: International Journal of Molecular Sciences

    Article Title: AQP3 Increases Intercellular Cohesion in NSCLC A549 Cell Spheroids through Exploratory Cell Protrusions

    doi: 10.3390/ijms22084287

    Figure Lengend Snippet: The effect of AQP3 on spatiotemporal dynamics of protrusions. ( A ) Polymerase chain reaction and ( B ) Quantitative real-time reverse transcription-polymerase chain reaction of the transcript levels of the organic hydroxyl transport genes. The data shown here represent three independent experiments, and the values represent the mean ± SEM of triplicate samples. The level of each mRNA was normalized to that of the GAPDH mRNA in the same sample and presented as the fold-change over that of the 2D culture control cells. The differences in expression levels were evaluated for significance using two-tailed t-tests with unequal variance. * p

    Article Snippet: In short, the cells on coverslips were fixed with 4% paraformaldehyde for 10 min and permeabilized with 0.15% Triton-X 100 for 5 min. Then, the cells were blocked for 1 h with the blocking solution of 3% bovine serum albumin in PBS and incubated with the primary antibody against AQP3 for 2 h at room temperature.

    Techniques: Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Expressing, Two Tailed Test