rabbit anti nherf1  (Alomone Labs)


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    Structured Review

    Alomone Labs rabbit anti nherf1
    Reduction in adaptor protein <t>NHERF1</t> in juvenile ependyma of LRP2-deficient mice En face lateral wall preparations for NHERF1 (green), LRP2 (purple), FOP (blue), and b-catenin (red). Single projections in single channels and merged channels configurations are shown of the apical and subapical ependymal cell compartment. In control ependyma, the apical cell compartment shows colocalization of LRP2 and FOP while the subapical compartment documents asymmetric distribution of NHERF1 towards the ciliary patch, the anterior cell side (indicated by arrows). In the Lrp2 -/267 ependyma, localization of FOP at the apical cell compartment is normal but the polarized enrichment of NHERF1 towards the ciliary patch in the subapical compartment is lost. Scale bar: 10 μm.
    Rabbit Anti Nherf1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti nherf1/product/Alomone Labs
    Average 94 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    rabbit anti nherf1 - by Bioz Stars, 2022-11
    94/100 stars

    Images

    1) Product Images from "LRP2 contributes to planar cell polarity-dependent coordination of motile cilia function"

    Article Title: LRP2 contributes to planar cell polarity-dependent coordination of motile cilia function

    Journal: bioRxiv

    doi: 10.1101/2022.07.12.499714

    Reduction in adaptor protein NHERF1 in juvenile ependyma of LRP2-deficient mice En face lateral wall preparations for NHERF1 (green), LRP2 (purple), FOP (blue), and b-catenin (red). Single projections in single channels and merged channels configurations are shown of the apical and subapical ependymal cell compartment. In control ependyma, the apical cell compartment shows colocalization of LRP2 and FOP while the subapical compartment documents asymmetric distribution of NHERF1 towards the ciliary patch, the anterior cell side (indicated by arrows). In the Lrp2 -/267 ependyma, localization of FOP at the apical cell compartment is normal but the polarized enrichment of NHERF1 towards the ciliary patch in the subapical compartment is lost. Scale bar: 10 μm.
    Figure Legend Snippet: Reduction in adaptor protein NHERF1 in juvenile ependyma of LRP2-deficient mice En face lateral wall preparations for NHERF1 (green), LRP2 (purple), FOP (blue), and b-catenin (red). Single projections in single channels and merged channels configurations are shown of the apical and subapical ependymal cell compartment. In control ependyma, the apical cell compartment shows colocalization of LRP2 and FOP while the subapical compartment documents asymmetric distribution of NHERF1 towards the ciliary patch, the anterior cell side (indicated by arrows). In the Lrp2 -/267 ependyma, localization of FOP at the apical cell compartment is normal but the polarized enrichment of NHERF1 towards the ciliary patch in the subapical compartment is lost. Scale bar: 10 μm.

    Techniques Used: Mouse Assay

    2) Product Images from "Parathyroid hormone initiates dynamic NHERF1 phosphorylation cycling and conformational changes that regulate NPT2A-dependent phosphate transport"

    Article Title: Parathyroid hormone initiates dynamic NHERF1 phosphorylation cycling and conformational changes that regulate NPT2A-dependent phosphate transport

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.RA119.007421

    NHERF1 Ser 290 dephosphorylation disrupts NPT2A binding. A ). TAMRA-labeled 22-residue NPT2A C-terminal peptide was incubated with the indicated form of NHERF1 for 15 min at room temperature. Affinities are shown as means ± S.E. ( n = 3; *, p
    Figure Legend Snippet: NHERF1 Ser 290 dephosphorylation disrupts NPT2A binding. A ). TAMRA-labeled 22-residue NPT2A C-terminal peptide was incubated with the indicated form of NHERF1 for 15 min at room temperature. Affinities are shown as means ± S.E. ( n = 3; *, p

    Techniques Used: De-Phosphorylation Assay, Binding Assay, Labeling, Incubation

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  • 94
    Alomone Labs rabbit anti nherf1
    Reduction in adaptor protein <t>NHERF1</t> in juvenile ependyma of LRP2-deficient mice En face lateral wall preparations for NHERF1 (green), LRP2 (purple), FOP (blue), and b-catenin (red). Single projections in single channels and merged channels configurations are shown of the apical and subapical ependymal cell compartment. In control ependyma, the apical cell compartment shows colocalization of LRP2 and FOP while the subapical compartment documents asymmetric distribution of NHERF1 towards the ciliary patch, the anterior cell side (indicated by arrows). In the Lrp2 -/267 ependyma, localization of FOP at the apical cell compartment is normal but the polarized enrichment of NHERF1 towards the ciliary patch in the subapical compartment is lost. Scale bar: 10 μm.
    Rabbit Anti Nherf1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti nherf1/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti nherf1 - by Bioz Stars, 2022-11
    94/100 stars
      Buy from Supplier

    Image Search Results


    Reduction in adaptor protein NHERF1 in juvenile ependyma of LRP2-deficient mice En face lateral wall preparations for NHERF1 (green), LRP2 (purple), FOP (blue), and b-catenin (red). Single projections in single channels and merged channels configurations are shown of the apical and subapical ependymal cell compartment. In control ependyma, the apical cell compartment shows colocalization of LRP2 and FOP while the subapical compartment documents asymmetric distribution of NHERF1 towards the ciliary patch, the anterior cell side (indicated by arrows). In the Lrp2 -/267 ependyma, localization of FOP at the apical cell compartment is normal but the polarized enrichment of NHERF1 towards the ciliary patch in the subapical compartment is lost. Scale bar: 10 μm.

    Journal: bioRxiv

    Article Title: LRP2 contributes to planar cell polarity-dependent coordination of motile cilia function

    doi: 10.1101/2022.07.12.499714

    Figure Lengend Snippet: Reduction in adaptor protein NHERF1 in juvenile ependyma of LRP2-deficient mice En face lateral wall preparations for NHERF1 (green), LRP2 (purple), FOP (blue), and b-catenin (red). Single projections in single channels and merged channels configurations are shown of the apical and subapical ependymal cell compartment. In control ependyma, the apical cell compartment shows colocalization of LRP2 and FOP while the subapical compartment documents asymmetric distribution of NHERF1 towards the ciliary patch, the anterior cell side (indicated by arrows). In the Lrp2 -/267 ependyma, localization of FOP at the apical cell compartment is normal but the polarized enrichment of NHERF1 towards the ciliary patch in the subapical compartment is lost. Scale bar: 10 μm.

    Article Snippet: The following primary antibodies were used: mouse IgG2b anti-acetylated-a-tubulin (1:1000; Sigma), mouse IgG1 anti-g-tubulin(1:400; Abcam), mouse IgG1 anti-b-catenin (1:500; BD Biosciences), mouse IgG2b anti-FGFR1OP (FOP; 1:1500; Abnova), rabbit anti-ZO1 (1:500; Invitrogen), rabbit anti-Arl13b (1:250; Proteintech), rabbit anti-Daple (1:100; IBL), rabbit anti-NHERF1 (1:300; Alomone labs), rabbit anti-CASMAP2 (1:100; Proteintech), rat anti-EB3 (1:100; Abcam), mouse anti-Dvl1 (1:100; Santa Cruz Biotechnology), rabbit anti-Vangl2 (1:300; gift from Mireille Montcouquiol, Neurocenter Magendie, Bordeaux, France), rabbit anti-Celsr1 (1:1000; gift from Elaine Fuchs, The Rockefeller University, New York, USA), guinea pig anti-LRP2 (1:1000; homemade).

    Techniques: Mouse Assay

    NHERF1 Ser 290 dephosphorylation disrupts NPT2A binding. A ). TAMRA-labeled 22-residue NPT2A C-terminal peptide was incubated with the indicated form of NHERF1 for 15 min at room temperature. Affinities are shown as means ± S.E. ( n = 3; *, p

    Journal: The Journal of Biological Chemistry

    Article Title: Parathyroid hormone initiates dynamic NHERF1 phosphorylation cycling and conformational changes that regulate NPT2A-dependent phosphate transport

    doi: 10.1074/jbc.RA119.007421

    Figure Lengend Snippet: NHERF1 Ser 290 dephosphorylation disrupts NPT2A binding. A ). TAMRA-labeled 22-residue NPT2A C-terminal peptide was incubated with the indicated form of NHERF1 for 15 min at room temperature. Affinities are shown as means ± S.E. ( n = 3; *, p

    Article Snippet: Antibodies were purchased from the following suppliers: rabbit anti-NHERF1 (Alomone Labs, APZ-006, lot 6AN0302); anti-HA (Cell Signaling Technology, 3724, lot 9); anti-GFP (Invitrogen, A6455, lot 1853896); anti-FLAG (Sigma, F7425, lot 085M4774V); anti-actin (Sigma, A1978, lot 076M4786V); and anti-phospho-Ser290 NHERF1 (pSer290) (GenScript, U5674CJ090, lot A318030013) generated against 284 ALVRSApSSDTSEEL297 ).

    Techniques: De-Phosphorylation Assay, Binding Assay, Labeling, Incubation