ep3 receptor  (Alomone Labs)


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    Structured Review

    Alomone Labs ep3 receptor
    The structure of agonists and antagonists of <t>EP3</t> receptors.
    Ep3 Receptor, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ep3 receptor/product/Alomone Labs
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    ep3 receptor - by Bioz Stars, 2022-08
    91/100 stars

    Images

    1) Product Images from "Prostaglandin E2 Impairs P2Y2/P2Y4 Receptor Signaling in Cerebellar Astrocytes via EP3 Receptors"

    Article Title: Prostaglandin E2 Impairs P2Y2/P2Y4 Receptor Signaling in Cerebellar Astrocytes via EP3 Receptors

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2017.00937

    The structure of agonists and antagonists of EP3 receptors.
    Figure Legend Snippet: The structure of agonists and antagonists of EP3 receptors.

    Techniques Used:

    Prostaglandin E 2 modulates UTP-induced ERK1/2 and PI3-K/Akt activation in astrocytes activated with LPS. Astrocytes were incubated in the presence or absence of LPS (10 ng/ml) for 24 h prior to analyzing the modulatory effect of PGE 2 on the increase in p-ERK1/2 (A) and p-Akt (B) induced by UTP (see Figure 6 ). The Western blots are representative of independent experiments and GAPDH was used as an internal loading control. The values are the means ± SEM from three independent experiments performed on different cultures and the results are expressed relative to the UTP response in each individual experiment. (C) LPS induced COX-2 and EP3 receptor expression in rat cerebellar astrocytes. The presence of COX-2, P2Y2R, P2Y4R, and EP3R proteins was detected in Western blots of total cell lysates. ∗∗∗ p
    Figure Legend Snippet: Prostaglandin E 2 modulates UTP-induced ERK1/2 and PI3-K/Akt activation in astrocytes activated with LPS. Astrocytes were incubated in the presence or absence of LPS (10 ng/ml) for 24 h prior to analyzing the modulatory effect of PGE 2 on the increase in p-ERK1/2 (A) and p-Akt (B) induced by UTP (see Figure 6 ). The Western blots are representative of independent experiments and GAPDH was used as an internal loading control. The values are the means ± SEM from three independent experiments performed on different cultures and the results are expressed relative to the UTP response in each individual experiment. (C) LPS induced COX-2 and EP3 receptor expression in rat cerebellar astrocytes. The presence of COX-2, P2Y2R, P2Y4R, and EP3R proteins was detected in Western blots of total cell lysates. ∗∗∗ p

    Techniques Used: Activation Assay, Incubation, Western Blot, Expressing

    Schematic representation of PGE 2 modulation of UTP signaling in rat cerebellar astrocytes. PGE 2 dampened the calcium responses induced by UTP stimulation of P2Y 2 /P2Y 4 , as well as reducing the UTP-induced ERK and Akt activation, and cell migration. The effect of PGE 2 was dependent on EP3 receptor interactions, and it involved EGFR transactivation and PI3K activity, although the involvement of nPKC could be not excluded.
    Figure Legend Snippet: Schematic representation of PGE 2 modulation of UTP signaling in rat cerebellar astrocytes. PGE 2 dampened the calcium responses induced by UTP stimulation of P2Y 2 /P2Y 4 , as well as reducing the UTP-induced ERK and Akt activation, and cell migration. The effect of PGE 2 was dependent on EP3 receptor interactions, and it involved EGFR transactivation and PI3K activity, although the involvement of nPKC could be not excluded.

    Techniques Used: Activation Assay, Migration, Activity Assay

    Effects of EP3 receptor ligands on UTP calcium responses in rat cerebellar astrocytes. (A) Sulprostone dampened the UTP calcium responses. Astrocytes were stimulated with UTP (100 μM), 30 nM Sulprostone or both these agonists, and the calcium responses were recorded. Where indicated, the cells were pre-incubated with sulprostone for 5 min before UTP stimulation. (B) Effect of L798106, an antagonist of EP3 receptors, on the calcium responses elicited by UTP and on PGE 2 modulation. Where indicated, cells were pre-incubated with L798106 (1 μM) or with L798106 and PGE 2 /sulprostone, prior to stimulation with the nucleotide. Typical calcium traces are shown, and the graphs represent the quantification of the responses obtained as described in Figure 1 and presented as the means ± SEM ( n = 227 cells from three different cultures). ∗∗∗ p
    Figure Legend Snippet: Effects of EP3 receptor ligands on UTP calcium responses in rat cerebellar astrocytes. (A) Sulprostone dampened the UTP calcium responses. Astrocytes were stimulated with UTP (100 μM), 30 nM Sulprostone or both these agonists, and the calcium responses were recorded. Where indicated, the cells were pre-incubated with sulprostone for 5 min before UTP stimulation. (B) Effect of L798106, an antagonist of EP3 receptors, on the calcium responses elicited by UTP and on PGE 2 modulation. Where indicated, cells were pre-incubated with L798106 (1 μM) or with L798106 and PGE 2 /sulprostone, prior to stimulation with the nucleotide. Typical calcium traces are shown, and the graphs represent the quantification of the responses obtained as described in Figure 1 and presented as the means ± SEM ( n = 227 cells from three different cultures). ∗∗∗ p

    Techniques Used: Incubation

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    Alomone Labs ep3 receptor
    The structure of agonists and antagonists of <t>EP3</t> receptors.
    Ep3 Receptor, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ep3 receptor/product/Alomone Labs
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ep3 receptor - by Bioz Stars, 2022-08
    91/100 stars
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    Image Search Results


    The structure of agonists and antagonists of EP3 receptors.

    Journal: Frontiers in Pharmacology

    Article Title: Prostaglandin E2 Impairs P2Y2/P2Y4 Receptor Signaling in Cerebellar Astrocytes via EP3 Receptors

    doi: 10.3389/fphar.2017.00937

    Figure Lengend Snippet: The structure of agonists and antagonists of EP3 receptors.

    Article Snippet: Antibodies against P2Y receptors, P2Y2 and P2Y4 receptors, and those against the EP3 receptor were from Alomone Labs (Jerusalem BioPark, Israel), while antibodies against GAPDH were purchased from Cell Signaling Technology (Beverly, MA, United States).

    Techniques:

    Prostaglandin E 2 modulates UTP-induced ERK1/2 and PI3-K/Akt activation in astrocytes activated with LPS. Astrocytes were incubated in the presence or absence of LPS (10 ng/ml) for 24 h prior to analyzing the modulatory effect of PGE 2 on the increase in p-ERK1/2 (A) and p-Akt (B) induced by UTP (see Figure 6 ). The Western blots are representative of independent experiments and GAPDH was used as an internal loading control. The values are the means ± SEM from three independent experiments performed on different cultures and the results are expressed relative to the UTP response in each individual experiment. (C) LPS induced COX-2 and EP3 receptor expression in rat cerebellar astrocytes. The presence of COX-2, P2Y2R, P2Y4R, and EP3R proteins was detected in Western blots of total cell lysates. ∗∗∗ p

    Journal: Frontiers in Pharmacology

    Article Title: Prostaglandin E2 Impairs P2Y2/P2Y4 Receptor Signaling in Cerebellar Astrocytes via EP3 Receptors

    doi: 10.3389/fphar.2017.00937

    Figure Lengend Snippet: Prostaglandin E 2 modulates UTP-induced ERK1/2 and PI3-K/Akt activation in astrocytes activated with LPS. Astrocytes were incubated in the presence or absence of LPS (10 ng/ml) for 24 h prior to analyzing the modulatory effect of PGE 2 on the increase in p-ERK1/2 (A) and p-Akt (B) induced by UTP (see Figure 6 ). The Western blots are representative of independent experiments and GAPDH was used as an internal loading control. The values are the means ± SEM from three independent experiments performed on different cultures and the results are expressed relative to the UTP response in each individual experiment. (C) LPS induced COX-2 and EP3 receptor expression in rat cerebellar astrocytes. The presence of COX-2, P2Y2R, P2Y4R, and EP3R proteins was detected in Western blots of total cell lysates. ∗∗∗ p

    Article Snippet: Antibodies against P2Y receptors, P2Y2 and P2Y4 receptors, and those against the EP3 receptor were from Alomone Labs (Jerusalem BioPark, Israel), while antibodies against GAPDH were purchased from Cell Signaling Technology (Beverly, MA, United States).

    Techniques: Activation Assay, Incubation, Western Blot, Expressing

    Schematic representation of PGE 2 modulation of UTP signaling in rat cerebellar astrocytes. PGE 2 dampened the calcium responses induced by UTP stimulation of P2Y 2 /P2Y 4 , as well as reducing the UTP-induced ERK and Akt activation, and cell migration. The effect of PGE 2 was dependent on EP3 receptor interactions, and it involved EGFR transactivation and PI3K activity, although the involvement of nPKC could be not excluded.

    Journal: Frontiers in Pharmacology

    Article Title: Prostaglandin E2 Impairs P2Y2/P2Y4 Receptor Signaling in Cerebellar Astrocytes via EP3 Receptors

    doi: 10.3389/fphar.2017.00937

    Figure Lengend Snippet: Schematic representation of PGE 2 modulation of UTP signaling in rat cerebellar astrocytes. PGE 2 dampened the calcium responses induced by UTP stimulation of P2Y 2 /P2Y 4 , as well as reducing the UTP-induced ERK and Akt activation, and cell migration. The effect of PGE 2 was dependent on EP3 receptor interactions, and it involved EGFR transactivation and PI3K activity, although the involvement of nPKC could be not excluded.

    Article Snippet: Antibodies against P2Y receptors, P2Y2 and P2Y4 receptors, and those against the EP3 receptor were from Alomone Labs (Jerusalem BioPark, Israel), while antibodies against GAPDH were purchased from Cell Signaling Technology (Beverly, MA, United States).

    Techniques: Activation Assay, Migration, Activity Assay

    Effects of EP3 receptor ligands on UTP calcium responses in rat cerebellar astrocytes. (A) Sulprostone dampened the UTP calcium responses. Astrocytes were stimulated with UTP (100 μM), 30 nM Sulprostone or both these agonists, and the calcium responses were recorded. Where indicated, the cells were pre-incubated with sulprostone for 5 min before UTP stimulation. (B) Effect of L798106, an antagonist of EP3 receptors, on the calcium responses elicited by UTP and on PGE 2 modulation. Where indicated, cells were pre-incubated with L798106 (1 μM) or with L798106 and PGE 2 /sulprostone, prior to stimulation with the nucleotide. Typical calcium traces are shown, and the graphs represent the quantification of the responses obtained as described in Figure 1 and presented as the means ± SEM ( n = 227 cells from three different cultures). ∗∗∗ p

    Journal: Frontiers in Pharmacology

    Article Title: Prostaglandin E2 Impairs P2Y2/P2Y4 Receptor Signaling in Cerebellar Astrocytes via EP3 Receptors

    doi: 10.3389/fphar.2017.00937

    Figure Lengend Snippet: Effects of EP3 receptor ligands on UTP calcium responses in rat cerebellar astrocytes. (A) Sulprostone dampened the UTP calcium responses. Astrocytes were stimulated with UTP (100 μM), 30 nM Sulprostone or both these agonists, and the calcium responses were recorded. Where indicated, the cells were pre-incubated with sulprostone for 5 min before UTP stimulation. (B) Effect of L798106, an antagonist of EP3 receptors, on the calcium responses elicited by UTP and on PGE 2 modulation. Where indicated, cells were pre-incubated with L798106 (1 μM) or with L798106 and PGE 2 /sulprostone, prior to stimulation with the nucleotide. Typical calcium traces are shown, and the graphs represent the quantification of the responses obtained as described in Figure 1 and presented as the means ± SEM ( n = 227 cells from three different cultures). ∗∗∗ p

    Article Snippet: Antibodies against P2Y receptors, P2Y2 and P2Y4 receptors, and those against the EP3 receptor were from Alomone Labs (Jerusalem BioPark, Israel), while antibodies against GAPDH were purchased from Cell Signaling Technology (Beverly, MA, United States).

    Techniques: Incubation