p2x4r fitc  (Alomone Labs)


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    Alomone Labs p2x4r fitc
    Determining IC50 of 5-BDBD. Plot indicates the effect of 5-BDBD at various concentrations (10 nM to 30 μM; log10 [nM] = −4.52 to −8) after <t>P2X4R</t> agonist ATP (1 μM) induced [Ca 2+ ]i response. Calculated IC50 was 3.8 × 10 −6 M.
    P2x4r Fitc, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p2x4r fitc/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p2x4r fitc - by Bioz Stars, 2022-05
    86/100 stars

    Images

    1) Product Images from "Neuroprotective and Neuro-rehabilitative Effects of Acute Purinergic Receptor P2X4 (P2X4R) Blockade after Ischemic Stroke"

    Article Title: Neuroprotective and Neuro-rehabilitative Effects of Acute Purinergic Receptor P2X4 (P2X4R) Blockade after Ischemic Stroke

    Journal: Experimental neurology

    doi: 10.1016/j.expneurol.2020.113308

    Determining IC50 of 5-BDBD. Plot indicates the effect of 5-BDBD at various concentrations (10 nM to 30 μM; log10 [nM] = −4.52 to −8) after P2X4R agonist ATP (1 μM) induced [Ca 2+ ]i response. Calculated IC50 was 3.8 × 10 −6 M.
    Figure Legend Snippet: Determining IC50 of 5-BDBD. Plot indicates the effect of 5-BDBD at various concentrations (10 nM to 30 μM; log10 [nM] = −4.52 to −8) after P2X4R agonist ATP (1 μM) induced [Ca 2+ ]i response. Calculated IC50 was 3.8 × 10 −6 M.

    Techniques Used:

    P2X4R-expressing IBA1+ myeloid cells are significantly reduced near the core/penumbra junction after 5-BDBD treatment. Images (top) and quantification (bottom) of P2X4R expression (green) in lba-1 positive cells (red) from immunostaining of brain sections 3 days after stroke. 20x magnification. DAPI (blue) = 4′,6-diamidino-2-phenylindole (nuclear stain). Scale bar 20 μm. Data are Mean ± SD (n=3 mice/group). *p
    Figure Legend Snippet: P2X4R-expressing IBA1+ myeloid cells are significantly reduced near the core/penumbra junction after 5-BDBD treatment. Images (top) and quantification (bottom) of P2X4R expression (green) in lba-1 positive cells (red) from immunostaining of brain sections 3 days after stroke. 20x magnification. DAPI (blue) = 4′,6-diamidino-2-phenylindole (nuclear stain). Scale bar 20 μm. Data are Mean ± SD (n=3 mice/group). *p

    Techniques Used: Expressing, Immunostaining, Staining, Mouse Assay

    5-BDBD treatment show differential response on BDNF levels at 3 days and at 30 days after stroke. Representative western blots are shown in the top panel. Effects of vehicle or 5-BDBD on pro and mature BDNF at (A) 3 days and (B) 30 days after stroke. C) Effects of vehicle and 5-BDBD treatment on pro/mature BDNF ratio 3 and 30 days after stroke. (D) P2X4R expression at 3 and 30 days after stroke were not different; Data are Mean ± SD (n=4–8 mice/group/time point). *p
    Figure Legend Snippet: 5-BDBD treatment show differential response on BDNF levels at 3 days and at 30 days after stroke. Representative western blots are shown in the top panel. Effects of vehicle or 5-BDBD on pro and mature BDNF at (A) 3 days and (B) 30 days after stroke. C) Effects of vehicle and 5-BDBD treatment on pro/mature BDNF ratio 3 and 30 days after stroke. (D) P2X4R expression at 3 and 30 days after stroke were not different; Data are Mean ± SD (n=4–8 mice/group/time point). *p

    Techniques Used: Western Blot, Expressing, Mouse Assay

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    • p2x4r fitc  (Alomone Labs)
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    Alomone Labs p2x4r fitc
    Determining IC50 of 5-BDBD. Plot indicates the effect of 5-BDBD at various concentrations (10 nM to 30 μM; log10 [nM] = −4.52 to −8) after <t>P2X4R</t> agonist ATP (1 μM) induced [Ca 2+ ]i response. Calculated IC50 was 3.8 × 10 −6 M.
    P2x4r Fitc, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p2x4r fitc/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p2x4r fitc - by Bioz Stars, 2022-05
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    Determining IC50 of 5-BDBD. Plot indicates the effect of 5-BDBD at various concentrations (10 nM to 30 μM; log10 [nM] = −4.52 to −8) after P2X4R agonist ATP (1 μM) induced [Ca 2+ ]i response. Calculated IC50 was 3.8 × 10 −6 M.

    Journal: Experimental neurology

    Article Title: Neuroprotective and Neuro-rehabilitative Effects of Acute Purinergic Receptor P2X4 (P2X4R) Blockade after Ischemic Stroke

    doi: 10.1016/j.expneurol.2020.113308

    Figure Lengend Snippet: Determining IC50 of 5-BDBD. Plot indicates the effect of 5-BDBD at various concentrations (10 nM to 30 μM; log10 [nM] = −4.52 to −8) after P2X4R agonist ATP (1 μM) induced [Ca 2+ ]i response. Calculated IC50 was 3.8 × 10 −6 M.

    Article Snippet: Briefly, all the cells isolated from the interphase 70%/30% Percoll gradient were washed and blocked with mouse Fc Block (clone 93, eBioscience, Thermo Fisher, Waltham, MA) before staining for 30 min in dark and cold conditions with primary antibody-conjugated fluorophores: CD45-eF450 (clone 2D1, eBioscience), CD11b-APCeF780 (clone M1/70, eBioscience), Ly6G-AF700 (clone 1A8, eBioscience), Ly6C-PerCP-Cy5.5 (clone HK1.4, BioLegend, San Diego, CA), CX3CR1 APC (Clone SA011F11, BioLegend), and P2X4R-FITC (AFR-024-F, Alomone Labs, Jerusalem, Israel).

    Techniques:

    P2X4R-expressing IBA1+ myeloid cells are significantly reduced near the core/penumbra junction after 5-BDBD treatment. Images (top) and quantification (bottom) of P2X4R expression (green) in lba-1 positive cells (red) from immunostaining of brain sections 3 days after stroke. 20x magnification. DAPI (blue) = 4′,6-diamidino-2-phenylindole (nuclear stain). Scale bar 20 μm. Data are Mean ± SD (n=3 mice/group). *p

    Journal: Experimental neurology

    Article Title: Neuroprotective and Neuro-rehabilitative Effects of Acute Purinergic Receptor P2X4 (P2X4R) Blockade after Ischemic Stroke

    doi: 10.1016/j.expneurol.2020.113308

    Figure Lengend Snippet: P2X4R-expressing IBA1+ myeloid cells are significantly reduced near the core/penumbra junction after 5-BDBD treatment. Images (top) and quantification (bottom) of P2X4R expression (green) in lba-1 positive cells (red) from immunostaining of brain sections 3 days after stroke. 20x magnification. DAPI (blue) = 4′,6-diamidino-2-phenylindole (nuclear stain). Scale bar 20 μm. Data are Mean ± SD (n=3 mice/group). *p

    Article Snippet: Briefly, all the cells isolated from the interphase 70%/30% Percoll gradient were washed and blocked with mouse Fc Block (clone 93, eBioscience, Thermo Fisher, Waltham, MA) before staining for 30 min in dark and cold conditions with primary antibody-conjugated fluorophores: CD45-eF450 (clone 2D1, eBioscience), CD11b-APCeF780 (clone M1/70, eBioscience), Ly6G-AF700 (clone 1A8, eBioscience), Ly6C-PerCP-Cy5.5 (clone HK1.4, BioLegend, San Diego, CA), CX3CR1 APC (Clone SA011F11, BioLegend), and P2X4R-FITC (AFR-024-F, Alomone Labs, Jerusalem, Israel).

    Techniques: Expressing, Immunostaining, Staining, Mouse Assay

    5-BDBD treatment show differential response on BDNF levels at 3 days and at 30 days after stroke. Representative western blots are shown in the top panel. Effects of vehicle or 5-BDBD on pro and mature BDNF at (A) 3 days and (B) 30 days after stroke. C) Effects of vehicle and 5-BDBD treatment on pro/mature BDNF ratio 3 and 30 days after stroke. (D) P2X4R expression at 3 and 30 days after stroke were not different; Data are Mean ± SD (n=4–8 mice/group/time point). *p

    Journal: Experimental neurology

    Article Title: Neuroprotective and Neuro-rehabilitative Effects of Acute Purinergic Receptor P2X4 (P2X4R) Blockade after Ischemic Stroke

    doi: 10.1016/j.expneurol.2020.113308

    Figure Lengend Snippet: 5-BDBD treatment show differential response on BDNF levels at 3 days and at 30 days after stroke. Representative western blots are shown in the top panel. Effects of vehicle or 5-BDBD on pro and mature BDNF at (A) 3 days and (B) 30 days after stroke. C) Effects of vehicle and 5-BDBD treatment on pro/mature BDNF ratio 3 and 30 days after stroke. (D) P2X4R expression at 3 and 30 days after stroke were not different; Data are Mean ± SD (n=4–8 mice/group/time point). *p

    Article Snippet: Briefly, all the cells isolated from the interphase 70%/30% Percoll gradient were washed and blocked with mouse Fc Block (clone 93, eBioscience, Thermo Fisher, Waltham, MA) before staining for 30 min in dark and cold conditions with primary antibody-conjugated fluorophores: CD45-eF450 (clone 2D1, eBioscience), CD11b-APCeF780 (clone M1/70, eBioscience), Ly6G-AF700 (clone 1A8, eBioscience), Ly6C-PerCP-Cy5.5 (clone HK1.4, BioLegend, San Diego, CA), CX3CR1 APC (Clone SA011F11, BioLegend), and P2X4R-FITC (AFR-024-F, Alomone Labs, Jerusalem, Israel).

    Techniques: Western Blot, Expressing, Mouse Assay