rabbit p2y1  (Alomone Labs)


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    Name:
    Anti P2Y1 Receptor extracellular Antibody
    Description:
    Anti P2Y1 Receptor extracellular Antibody APR 021 is a highly specific antibody directed against an extracellular epitope of the human P2RY1 The antibody can be used in western blot immunohistochemistry indirect flow cytometry and live cell imaging applications It has been designed to recognize P2RY1 from mouse rat and human samples
    Catalog Number:
    APR-021
    Price:
    397.0
    Category:
    Primary Antibody
    Applications:
    Immunocytochemistry, Immunofluorescence, Indirect Flow Cytometry, Immunohistochemistry, Live Cell Imaging, Western Blot
    Purity:
    Affinity purified on immobilized antigen.
    Immunogen:
    Synthetic peptide
    Size:
    25 mcl
    Antibody Type:
    Polyclonal Primary Antibodies
    Format:
    Lyophilized Powder
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
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    Structured Review

    Alomone Labs rabbit p2y1
    Anti P2Y1 Receptor extracellular Antibody
    Anti P2Y1 Receptor extracellular Antibody APR 021 is a highly specific antibody directed against an extracellular epitope of the human P2RY1 The antibody can be used in western blot immunohistochemistry indirect flow cytometry and live cell imaging applications It has been designed to recognize P2RY1 from mouse rat and human samples
    https://www.bioz.com/result/rabbit p2y1/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit p2y1 - by Bioz Stars, 2021-09
    92/100 stars

    Images

    1) Product Images from "Autocrine stimulation of P2Y1 receptors is part of the purinergic signaling mechanism that regulates T cell activation"

    Article Title: Autocrine stimulation of P2Y1 receptors is part of the purinergic signaling mechanism that regulates T cell activation

    Journal: Purinergic Signalling

    doi: 10.1007/s11302-019-09653-6

    P2Y1 receptors promote IL-2 expression in response to TCR/CD28 stimulation. a CD4 T cells were treated for 10 min with the indicated concentrations of the P2Y1 antagonist MRS2279 and stimulated for 5 min with anti-CD3/CD28 antibody-coated microbeads, and ERK1/2 MAPK activation was determined by immunoblotting with phosphospecific anti-ERK1/2 antibodies. Antibodies recognizing total ERK1/2 were used to verify equal protein loading. Data represent mean values ± SD of separate experiments ( n = 3); * p
    Figure Legend Snippet: P2Y1 receptors promote IL-2 expression in response to TCR/CD28 stimulation. a CD4 T cells were treated for 10 min with the indicated concentrations of the P2Y1 antagonist MRS2279 and stimulated for 5 min with anti-CD3/CD28 antibody-coated microbeads, and ERK1/2 MAPK activation was determined by immunoblotting with phosphospecific anti-ERK1/2 antibodies. Antibodies recognizing total ERK1/2 were used to verify equal protein loading. Data represent mean values ± SD of separate experiments ( n = 3); * p

    Techniques Used: Expressing, Activation Assay

    Proposed mechanisms by which P2X4 and P2Y1 receptors synergize to regulate T cells. Stimulation of their T cells via T cell receptor (TCR) and CD28 coreceptors triggers mitochondrial ATP production and ATP release through pannexin-1 channels (Panx1). The released ATP activates P2X4 receptors that promote Ca 2+ influx. Hydrolysis of extracellular ATP by ectonucleotide triphosphate diphosphohydrolases (ENTPD) generates ADP that activates P2Y1 receptors that contribute to the increase in intracellular Ca 2+ concentrations. Increased mitochondrial activity, P2X4 and P2Y1 receptor Ca2+ signaling increases ATP release and fuel an autocrine feed-forward signaling mechanism that enhances T cell activation, and subsequent downstream signaling pathways that culminate in IL-2 transcription and effector functions involved in inflammation and host defense
    Figure Legend Snippet: Proposed mechanisms by which P2X4 and P2Y1 receptors synergize to regulate T cells. Stimulation of their T cells via T cell receptor (TCR) and CD28 coreceptors triggers mitochondrial ATP production and ATP release through pannexin-1 channels (Panx1). The released ATP activates P2X4 receptors that promote Ca 2+ influx. Hydrolysis of extracellular ATP by ectonucleotide triphosphate diphosphohydrolases (ENTPD) generates ADP that activates P2Y1 receptors that contribute to the increase in intracellular Ca 2+ concentrations. Increased mitochondrial activity, P2X4 and P2Y1 receptor Ca2+ signaling increases ATP release and fuel an autocrine feed-forward signaling mechanism that enhances T cell activation, and subsequent downstream signaling pathways that culminate in IL-2 transcription and effector functions involved in inflammation and host defense

    Techniques Used: Activity Assay, Activation Assay

    CD4 T cells express ATP-selective P2X and ADP-selective P2Y receptors. a P2 receptor mRNA levels in purified CD4 T cells were determined with qPCR using CD4 T cells isolated from healthy human subjects or Jurkat T cells (mean ± SD, n = 3; n.d., not detected). b Total protein expression of P2Y1 and P2Y12 receptors in human CD4 T cells before and after TCR/CD28 stimulation. The results shown are representative of n = 3 experiments with cells from different subjects. c – d Cell surface expression of P2Y1 and P2Y12 receptors on human CD4 T cells was assessed with flow cytometry before or 72 h after TCR/CD28 stimulation. Representative histograms ( c ) and summarized data ( d ) of unstimulated (n = 5) or stimulated (n = 4) cell preparations with cells from different donors are shown (* p
    Figure Legend Snippet: CD4 T cells express ATP-selective P2X and ADP-selective P2Y receptors. a P2 receptor mRNA levels in purified CD4 T cells were determined with qPCR using CD4 T cells isolated from healthy human subjects or Jurkat T cells (mean ± SD, n = 3; n.d., not detected). b Total protein expression of P2Y1 and P2Y12 receptors in human CD4 T cells before and after TCR/CD28 stimulation. The results shown are representative of n = 3 experiments with cells from different subjects. c – d Cell surface expression of P2Y1 and P2Y12 receptors on human CD4 T cells was assessed with flow cytometry before or 72 h after TCR/CD28 stimulation. Representative histograms ( c ) and summarized data ( d ) of unstimulated (n = 5) or stimulated (n = 4) cell preparations with cells from different donors are shown (* p

    Techniques Used: Purification, Real-time Polymerase Chain Reaction, Isolation, Expressing, Flow Cytometry, Cytometry

    P2Y1 and P2X4 receptors activate mitochondria in response to TCR/CD28 stimulation. a Jurkat cells expressing the mitochondrial Ca 2+ indicator mito-CAR-GECO1 were pretreated for 10 min with specific antagonists of P2X4 (5-BDBD, 10 μM) or P2Y1 (MRS2279, 10 μM) receptors or with the general P2 receptor antagonist suramin (100 μM). Then, cells were stimulated by TCR/CD28 cross-linking, and changes in mitochondrial Ca 2+ uptake were recorded over time using fluorescence microscopy. Traces of individual cells are shown in gray, and the averages of all cells acquired ( n = 30–80) are shown in red. Data shown are representative of independent experiments ( n ≥ 3). b Averaged mean fluorescence values (+ SEM) of cells ( n = 30-80) from different experiments ( n ≥ 3 experiments). c Peak mitochondrial Ca 2+ levels following TCR/CD28 stimulation; mean ± SEM; * p
    Figure Legend Snippet: P2Y1 and P2X4 receptors activate mitochondria in response to TCR/CD28 stimulation. a Jurkat cells expressing the mitochondrial Ca 2+ indicator mito-CAR-GECO1 were pretreated for 10 min with specific antagonists of P2X4 (5-BDBD, 10 μM) or P2Y1 (MRS2279, 10 μM) receptors or with the general P2 receptor antagonist suramin (100 μM). Then, cells were stimulated by TCR/CD28 cross-linking, and changes in mitochondrial Ca 2+ uptake were recorded over time using fluorescence microscopy. Traces of individual cells are shown in gray, and the averages of all cells acquired ( n = 30–80) are shown in red. Data shown are representative of independent experiments ( n ≥ 3). b Averaged mean fluorescence values (+ SEM) of cells ( n = 30-80) from different experiments ( n ≥ 3 experiments). c Peak mitochondrial Ca 2+ levels following TCR/CD28 stimulation; mean ± SEM; * p

    Techniques Used: Expressing, Fluorescence, Microscopy

    P2Y1 and P2X4 receptors regulate cytosolic Ca 2+ signaling in response to TCR/CD28 stimulation. a Primary human CD4 T cells were loaded with the cytosolic Ca 2+ probe Fluo-4 AM, pretreated for 10 min with specific antagonists of P2X4 (5-BDBD, 10 μM) or P2Y1 (MRS2279, 10 μM) receptors or with the general P2 receptor antagonist suramin (100 μM). Then, cells were stimulated by TCR/CD28 cross-linking, and changes in cytosolic Ca 2+ levels were recorded over time using live-cell fluorescence microscopy. Traces of individual cells are shown in gray, and averages of all cells studied ( n = 120–350) are shown in red. Data shown are representative of different experiments ( n ≥ 3) with cells from different donors. b Averaged mean fluorescence values (+ SEM) of cells from at least three different donors ( n = 120-350 per experiment). c Peak Ca 2+ levels following TCR/CD28 stimulation; mean ± SEM; * p
    Figure Legend Snippet: P2Y1 and P2X4 receptors regulate cytosolic Ca 2+ signaling in response to TCR/CD28 stimulation. a Primary human CD4 T cells were loaded with the cytosolic Ca 2+ probe Fluo-4 AM, pretreated for 10 min with specific antagonists of P2X4 (5-BDBD, 10 μM) or P2Y1 (MRS2279, 10 μM) receptors or with the general P2 receptor antagonist suramin (100 μM). Then, cells were stimulated by TCR/CD28 cross-linking, and changes in cytosolic Ca 2+ levels were recorded over time using live-cell fluorescence microscopy. Traces of individual cells are shown in gray, and averages of all cells studied ( n = 120–350) are shown in red. Data shown are representative of different experiments ( n ≥ 3) with cells from different donors. b Averaged mean fluorescence values (+ SEM) of cells from at least three different donors ( n = 120-350 per experiment). c Peak Ca 2+ levels following TCR/CD28 stimulation; mean ± SEM; * p

    Techniques Used: Fluorescence, Microscopy

    Related Articles

    other:

    Article Title: Autocrine stimulation of P2Y1 receptors is part of the purinergic signaling mechanism that regulates T cell activation
    Article Snippet: Rabbit P2Y1 (Cat. # APR-021) and P2Y12 (Cat. # APR-020) antibodies recognizing extracellular epitopes of human P2Y1 and P2Y12 receptors, respectively, were purchased from Alomone Labs (Jerusalem, Israel).

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    Alomone Labs rabbit p2y1
    <t>P2Y1</t> receptors promote IL-2 expression in response to TCR/CD28 stimulation. a CD4 T cells were treated for 10 min with the indicated concentrations of the P2Y1 antagonist MRS2279 and stimulated for 5 min with anti-CD3/CD28 antibody-coated microbeads, and ERK1/2 MAPK activation was determined by immunoblotting with phosphospecific anti-ERK1/2 antibodies. Antibodies recognizing total ERK1/2 were used to verify equal protein loading. Data represent mean values ± SD of separate experiments ( n = 3); * p
    Rabbit P2y1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit p2y1/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit p2y1 - by Bioz Stars, 2021-09
    92/100 stars
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    P2Y1 receptors promote IL-2 expression in response to TCR/CD28 stimulation. a CD4 T cells were treated for 10 min with the indicated concentrations of the P2Y1 antagonist MRS2279 and stimulated for 5 min with anti-CD3/CD28 antibody-coated microbeads, and ERK1/2 MAPK activation was determined by immunoblotting with phosphospecific anti-ERK1/2 antibodies. Antibodies recognizing total ERK1/2 were used to verify equal protein loading. Data represent mean values ± SD of separate experiments ( n = 3); * p

    Journal: Purinergic Signalling

    Article Title: Autocrine stimulation of P2Y1 receptors is part of the purinergic signaling mechanism that regulates T cell activation

    doi: 10.1007/s11302-019-09653-6

    Figure Lengend Snippet: P2Y1 receptors promote IL-2 expression in response to TCR/CD28 stimulation. a CD4 T cells were treated for 10 min with the indicated concentrations of the P2Y1 antagonist MRS2279 and stimulated for 5 min with anti-CD3/CD28 antibody-coated microbeads, and ERK1/2 MAPK activation was determined by immunoblotting with phosphospecific anti-ERK1/2 antibodies. Antibodies recognizing total ERK1/2 were used to verify equal protein loading. Data represent mean values ± SD of separate experiments ( n = 3); * p

    Article Snippet: Rabbit P2Y1 (Cat. # APR-021) and P2Y12 (Cat. # APR-020) antibodies recognizing extracellular epitopes of human P2Y1 and P2Y12 receptors, respectively, were purchased from Alomone Labs (Jerusalem, Israel).

    Techniques: Expressing, Activation Assay

    Proposed mechanisms by which P2X4 and P2Y1 receptors synergize to regulate T cells. Stimulation of their T cells via T cell receptor (TCR) and CD28 coreceptors triggers mitochondrial ATP production and ATP release through pannexin-1 channels (Panx1). The released ATP activates P2X4 receptors that promote Ca 2+ influx. Hydrolysis of extracellular ATP by ectonucleotide triphosphate diphosphohydrolases (ENTPD) generates ADP that activates P2Y1 receptors that contribute to the increase in intracellular Ca 2+ concentrations. Increased mitochondrial activity, P2X4 and P2Y1 receptor Ca2+ signaling increases ATP release and fuel an autocrine feed-forward signaling mechanism that enhances T cell activation, and subsequent downstream signaling pathways that culminate in IL-2 transcription and effector functions involved in inflammation and host defense

    Journal: Purinergic Signalling

    Article Title: Autocrine stimulation of P2Y1 receptors is part of the purinergic signaling mechanism that regulates T cell activation

    doi: 10.1007/s11302-019-09653-6

    Figure Lengend Snippet: Proposed mechanisms by which P2X4 and P2Y1 receptors synergize to regulate T cells. Stimulation of their T cells via T cell receptor (TCR) and CD28 coreceptors triggers mitochondrial ATP production and ATP release through pannexin-1 channels (Panx1). The released ATP activates P2X4 receptors that promote Ca 2+ influx. Hydrolysis of extracellular ATP by ectonucleotide triphosphate diphosphohydrolases (ENTPD) generates ADP that activates P2Y1 receptors that contribute to the increase in intracellular Ca 2+ concentrations. Increased mitochondrial activity, P2X4 and P2Y1 receptor Ca2+ signaling increases ATP release and fuel an autocrine feed-forward signaling mechanism that enhances T cell activation, and subsequent downstream signaling pathways that culminate in IL-2 transcription and effector functions involved in inflammation and host defense

    Article Snippet: Rabbit P2Y1 (Cat. # APR-021) and P2Y12 (Cat. # APR-020) antibodies recognizing extracellular epitopes of human P2Y1 and P2Y12 receptors, respectively, were purchased from Alomone Labs (Jerusalem, Israel).

    Techniques: Activity Assay, Activation Assay

    CD4 T cells express ATP-selective P2X and ADP-selective P2Y receptors. a P2 receptor mRNA levels in purified CD4 T cells were determined with qPCR using CD4 T cells isolated from healthy human subjects or Jurkat T cells (mean ± SD, n = 3; n.d., not detected). b Total protein expression of P2Y1 and P2Y12 receptors in human CD4 T cells before and after TCR/CD28 stimulation. The results shown are representative of n = 3 experiments with cells from different subjects. c – d Cell surface expression of P2Y1 and P2Y12 receptors on human CD4 T cells was assessed with flow cytometry before or 72 h after TCR/CD28 stimulation. Representative histograms ( c ) and summarized data ( d ) of unstimulated (n = 5) or stimulated (n = 4) cell preparations with cells from different donors are shown (* p

    Journal: Purinergic Signalling

    Article Title: Autocrine stimulation of P2Y1 receptors is part of the purinergic signaling mechanism that regulates T cell activation

    doi: 10.1007/s11302-019-09653-6

    Figure Lengend Snippet: CD4 T cells express ATP-selective P2X and ADP-selective P2Y receptors. a P2 receptor mRNA levels in purified CD4 T cells were determined with qPCR using CD4 T cells isolated from healthy human subjects or Jurkat T cells (mean ± SD, n = 3; n.d., not detected). b Total protein expression of P2Y1 and P2Y12 receptors in human CD4 T cells before and after TCR/CD28 stimulation. The results shown are representative of n = 3 experiments with cells from different subjects. c – d Cell surface expression of P2Y1 and P2Y12 receptors on human CD4 T cells was assessed with flow cytometry before or 72 h after TCR/CD28 stimulation. Representative histograms ( c ) and summarized data ( d ) of unstimulated (n = 5) or stimulated (n = 4) cell preparations with cells from different donors are shown (* p

    Article Snippet: Rabbit P2Y1 (Cat. # APR-021) and P2Y12 (Cat. # APR-020) antibodies recognizing extracellular epitopes of human P2Y1 and P2Y12 receptors, respectively, were purchased from Alomone Labs (Jerusalem, Israel).

    Techniques: Purification, Real-time Polymerase Chain Reaction, Isolation, Expressing, Flow Cytometry, Cytometry

    P2Y1 and P2X4 receptors activate mitochondria in response to TCR/CD28 stimulation. a Jurkat cells expressing the mitochondrial Ca 2+ indicator mito-CAR-GECO1 were pretreated for 10 min with specific antagonists of P2X4 (5-BDBD, 10 μM) or P2Y1 (MRS2279, 10 μM) receptors or with the general P2 receptor antagonist suramin (100 μM). Then, cells were stimulated by TCR/CD28 cross-linking, and changes in mitochondrial Ca 2+ uptake were recorded over time using fluorescence microscopy. Traces of individual cells are shown in gray, and the averages of all cells acquired ( n = 30–80) are shown in red. Data shown are representative of independent experiments ( n ≥ 3). b Averaged mean fluorescence values (+ SEM) of cells ( n = 30-80) from different experiments ( n ≥ 3 experiments). c Peak mitochondrial Ca 2+ levels following TCR/CD28 stimulation; mean ± SEM; * p

    Journal: Purinergic Signalling

    Article Title: Autocrine stimulation of P2Y1 receptors is part of the purinergic signaling mechanism that regulates T cell activation

    doi: 10.1007/s11302-019-09653-6

    Figure Lengend Snippet: P2Y1 and P2X4 receptors activate mitochondria in response to TCR/CD28 stimulation. a Jurkat cells expressing the mitochondrial Ca 2+ indicator mito-CAR-GECO1 were pretreated for 10 min with specific antagonists of P2X4 (5-BDBD, 10 μM) or P2Y1 (MRS2279, 10 μM) receptors or with the general P2 receptor antagonist suramin (100 μM). Then, cells were stimulated by TCR/CD28 cross-linking, and changes in mitochondrial Ca 2+ uptake were recorded over time using fluorescence microscopy. Traces of individual cells are shown in gray, and the averages of all cells acquired ( n = 30–80) are shown in red. Data shown are representative of independent experiments ( n ≥ 3). b Averaged mean fluorescence values (+ SEM) of cells ( n = 30-80) from different experiments ( n ≥ 3 experiments). c Peak mitochondrial Ca 2+ levels following TCR/CD28 stimulation; mean ± SEM; * p

    Article Snippet: Rabbit P2Y1 (Cat. # APR-021) and P2Y12 (Cat. # APR-020) antibodies recognizing extracellular epitopes of human P2Y1 and P2Y12 receptors, respectively, were purchased from Alomone Labs (Jerusalem, Israel).

    Techniques: Expressing, Fluorescence, Microscopy

    P2Y1 and P2X4 receptors regulate cytosolic Ca 2+ signaling in response to TCR/CD28 stimulation. a Primary human CD4 T cells were loaded with the cytosolic Ca 2+ probe Fluo-4 AM, pretreated for 10 min with specific antagonists of P2X4 (5-BDBD, 10 μM) or P2Y1 (MRS2279, 10 μM) receptors or with the general P2 receptor antagonist suramin (100 μM). Then, cells were stimulated by TCR/CD28 cross-linking, and changes in cytosolic Ca 2+ levels were recorded over time using live-cell fluorescence microscopy. Traces of individual cells are shown in gray, and averages of all cells studied ( n = 120–350) are shown in red. Data shown are representative of different experiments ( n ≥ 3) with cells from different donors. b Averaged mean fluorescence values (+ SEM) of cells from at least three different donors ( n = 120-350 per experiment). c Peak Ca 2+ levels following TCR/CD28 stimulation; mean ± SEM; * p

    Journal: Purinergic Signalling

    Article Title: Autocrine stimulation of P2Y1 receptors is part of the purinergic signaling mechanism that regulates T cell activation

    doi: 10.1007/s11302-019-09653-6

    Figure Lengend Snippet: P2Y1 and P2X4 receptors regulate cytosolic Ca 2+ signaling in response to TCR/CD28 stimulation. a Primary human CD4 T cells were loaded with the cytosolic Ca 2+ probe Fluo-4 AM, pretreated for 10 min with specific antagonists of P2X4 (5-BDBD, 10 μM) or P2Y1 (MRS2279, 10 μM) receptors or with the general P2 receptor antagonist suramin (100 μM). Then, cells were stimulated by TCR/CD28 cross-linking, and changes in cytosolic Ca 2+ levels were recorded over time using live-cell fluorescence microscopy. Traces of individual cells are shown in gray, and averages of all cells studied ( n = 120–350) are shown in red. Data shown are representative of different experiments ( n ≥ 3) with cells from different donors. b Averaged mean fluorescence values (+ SEM) of cells from at least three different donors ( n = 120-350 per experiment). c Peak Ca 2+ levels following TCR/CD28 stimulation; mean ± SEM; * p

    Article Snippet: Rabbit P2Y1 (Cat. # APR-021) and P2Y12 (Cat. # APR-020) antibodies recognizing extracellular epitopes of human P2Y1 and P2Y12 receptors, respectively, were purchased from Alomone Labs (Jerusalem, Israel).

    Techniques: Fluorescence, Microscopy