rabbit p2y1  (Alomone Labs)


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    Alomone Labs rabbit p2y1
    <t>P2Y1</t> receptors promote IL-2 expression in response to TCR/CD28 stimulation. a CD4 T cells were treated for 10 min with the indicated concentrations of the P2Y1 antagonist MRS2279 and stimulated for 5 min with anti-CD3/CD28 antibody-coated microbeads, and ERK1/2 MAPK activation was determined by immunoblotting with phosphospecific anti-ERK1/2 antibodies. Antibodies recognizing total ERK1/2 were used to verify equal protein loading. Data represent mean values ± SD of separate experiments ( n = 3); * p
    Rabbit P2y1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit p2y1/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit p2y1 - by Bioz Stars, 2022-01
    92/100 stars

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    1) Product Images from "Autocrine stimulation of P2Y1 receptors is part of the purinergic signaling mechanism that regulates T cell activation"

    Article Title: Autocrine stimulation of P2Y1 receptors is part of the purinergic signaling mechanism that regulates T cell activation

    Journal: Purinergic Signalling

    doi: 10.1007/s11302-019-09653-6

    P2Y1 receptors promote IL-2 expression in response to TCR/CD28 stimulation. a CD4 T cells were treated for 10 min with the indicated concentrations of the P2Y1 antagonist MRS2279 and stimulated for 5 min with anti-CD3/CD28 antibody-coated microbeads, and ERK1/2 MAPK activation was determined by immunoblotting with phosphospecific anti-ERK1/2 antibodies. Antibodies recognizing total ERK1/2 were used to verify equal protein loading. Data represent mean values ± SD of separate experiments ( n = 3); * p
    Figure Legend Snippet: P2Y1 receptors promote IL-2 expression in response to TCR/CD28 stimulation. a CD4 T cells were treated for 10 min with the indicated concentrations of the P2Y1 antagonist MRS2279 and stimulated for 5 min with anti-CD3/CD28 antibody-coated microbeads, and ERK1/2 MAPK activation was determined by immunoblotting with phosphospecific anti-ERK1/2 antibodies. Antibodies recognizing total ERK1/2 were used to verify equal protein loading. Data represent mean values ± SD of separate experiments ( n = 3); * p

    Techniques Used: Expressing, Activation Assay

    Proposed mechanisms by which P2X4 and P2Y1 receptors synergize to regulate T cells. Stimulation of their T cells via T cell receptor (TCR) and CD28 coreceptors triggers mitochondrial ATP production and ATP release through pannexin-1 channels (Panx1). The released ATP activates P2X4 receptors that promote Ca 2+ influx. Hydrolysis of extracellular ATP by ectonucleotide triphosphate diphosphohydrolases (ENTPD) generates ADP that activates P2Y1 receptors that contribute to the increase in intracellular Ca 2+ concentrations. Increased mitochondrial activity, P2X4 and P2Y1 receptor Ca2+ signaling increases ATP release and fuel an autocrine feed-forward signaling mechanism that enhances T cell activation, and subsequent downstream signaling pathways that culminate in IL-2 transcription and effector functions involved in inflammation and host defense
    Figure Legend Snippet: Proposed mechanisms by which P2X4 and P2Y1 receptors synergize to regulate T cells. Stimulation of their T cells via T cell receptor (TCR) and CD28 coreceptors triggers mitochondrial ATP production and ATP release through pannexin-1 channels (Panx1). The released ATP activates P2X4 receptors that promote Ca 2+ influx. Hydrolysis of extracellular ATP by ectonucleotide triphosphate diphosphohydrolases (ENTPD) generates ADP that activates P2Y1 receptors that contribute to the increase in intracellular Ca 2+ concentrations. Increased mitochondrial activity, P2X4 and P2Y1 receptor Ca2+ signaling increases ATP release and fuel an autocrine feed-forward signaling mechanism that enhances T cell activation, and subsequent downstream signaling pathways that culminate in IL-2 transcription and effector functions involved in inflammation and host defense

    Techniques Used: Activity Assay, Activation Assay

    CD4 T cells express ATP-selective P2X and ADP-selective P2Y receptors. a P2 receptor mRNA levels in purified CD4 T cells were determined with qPCR using CD4 T cells isolated from healthy human subjects or Jurkat T cells (mean ± SD, n = 3; n.d., not detected). b Total protein expression of P2Y1 and P2Y12 receptors in human CD4 T cells before and after TCR/CD28 stimulation. The results shown are representative of n = 3 experiments with cells from different subjects. c – d Cell surface expression of P2Y1 and P2Y12 receptors on human CD4 T cells was assessed with flow cytometry before or 72 h after TCR/CD28 stimulation. Representative histograms ( c ) and summarized data ( d ) of unstimulated (n = 5) or stimulated (n = 4) cell preparations with cells from different donors are shown (* p
    Figure Legend Snippet: CD4 T cells express ATP-selective P2X and ADP-selective P2Y receptors. a P2 receptor mRNA levels in purified CD4 T cells were determined with qPCR using CD4 T cells isolated from healthy human subjects or Jurkat T cells (mean ± SD, n = 3; n.d., not detected). b Total protein expression of P2Y1 and P2Y12 receptors in human CD4 T cells before and after TCR/CD28 stimulation. The results shown are representative of n = 3 experiments with cells from different subjects. c – d Cell surface expression of P2Y1 and P2Y12 receptors on human CD4 T cells was assessed with flow cytometry before or 72 h after TCR/CD28 stimulation. Representative histograms ( c ) and summarized data ( d ) of unstimulated (n = 5) or stimulated (n = 4) cell preparations with cells from different donors are shown (* p

    Techniques Used: Purification, Real-time Polymerase Chain Reaction, Isolation, Expressing, Flow Cytometry, Cytometry

    P2Y1 and P2X4 receptors activate mitochondria in response to TCR/CD28 stimulation. a Jurkat cells expressing the mitochondrial Ca 2+ indicator mito-CAR-GECO1 were pretreated for 10 min with specific antagonists of P2X4 (5-BDBD, 10 μM) or P2Y1 (MRS2279, 10 μM) receptors or with the general P2 receptor antagonist suramin (100 μM). Then, cells were stimulated by TCR/CD28 cross-linking, and changes in mitochondrial Ca 2+ uptake were recorded over time using fluorescence microscopy. Traces of individual cells are shown in gray, and the averages of all cells acquired ( n = 30–80) are shown in red. Data shown are representative of independent experiments ( n ≥ 3). b Averaged mean fluorescence values (+ SEM) of cells ( n = 30-80) from different experiments ( n ≥ 3 experiments). c Peak mitochondrial Ca 2+ levels following TCR/CD28 stimulation; mean ± SEM; * p
    Figure Legend Snippet: P2Y1 and P2X4 receptors activate mitochondria in response to TCR/CD28 stimulation. a Jurkat cells expressing the mitochondrial Ca 2+ indicator mito-CAR-GECO1 were pretreated for 10 min with specific antagonists of P2X4 (5-BDBD, 10 μM) or P2Y1 (MRS2279, 10 μM) receptors or with the general P2 receptor antagonist suramin (100 μM). Then, cells were stimulated by TCR/CD28 cross-linking, and changes in mitochondrial Ca 2+ uptake were recorded over time using fluorescence microscopy. Traces of individual cells are shown in gray, and the averages of all cells acquired ( n = 30–80) are shown in red. Data shown are representative of independent experiments ( n ≥ 3). b Averaged mean fluorescence values (+ SEM) of cells ( n = 30-80) from different experiments ( n ≥ 3 experiments). c Peak mitochondrial Ca 2+ levels following TCR/CD28 stimulation; mean ± SEM; * p

    Techniques Used: Expressing, Fluorescence, Microscopy

    P2Y1 and P2X4 receptors regulate cytosolic Ca 2+ signaling in response to TCR/CD28 stimulation. a Primary human CD4 T cells were loaded with the cytosolic Ca 2+ probe Fluo-4 AM, pretreated for 10 min with specific antagonists of P2X4 (5-BDBD, 10 μM) or P2Y1 (MRS2279, 10 μM) receptors or with the general P2 receptor antagonist suramin (100 μM). Then, cells were stimulated by TCR/CD28 cross-linking, and changes in cytosolic Ca 2+ levels were recorded over time using live-cell fluorescence microscopy. Traces of individual cells are shown in gray, and averages of all cells studied ( n = 120–350) are shown in red. Data shown are representative of different experiments ( n ≥ 3) with cells from different donors. b Averaged mean fluorescence values (+ SEM) of cells from at least three different donors ( n = 120-350 per experiment). c Peak Ca 2+ levels following TCR/CD28 stimulation; mean ± SEM; * p
    Figure Legend Snippet: P2Y1 and P2X4 receptors regulate cytosolic Ca 2+ signaling in response to TCR/CD28 stimulation. a Primary human CD4 T cells were loaded with the cytosolic Ca 2+ probe Fluo-4 AM, pretreated for 10 min with specific antagonists of P2X4 (5-BDBD, 10 μM) or P2Y1 (MRS2279, 10 μM) receptors or with the general P2 receptor antagonist suramin (100 μM). Then, cells were stimulated by TCR/CD28 cross-linking, and changes in cytosolic Ca 2+ levels were recorded over time using live-cell fluorescence microscopy. Traces of individual cells are shown in gray, and averages of all cells studied ( n = 120–350) are shown in red. Data shown are representative of different experiments ( n ≥ 3) with cells from different donors. b Averaged mean fluorescence values (+ SEM) of cells from at least three different donors ( n = 120-350 per experiment). c Peak Ca 2+ levels following TCR/CD28 stimulation; mean ± SEM; * p

    Techniques Used: Fluorescence, Microscopy

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    Alomone Labs rabbit p2y1
    <t>P2Y1</t> receptors promote IL-2 expression in response to TCR/CD28 stimulation. a CD4 T cells were treated for 10 min with the indicated concentrations of the P2Y1 antagonist MRS2279 and stimulated for 5 min with anti-CD3/CD28 antibody-coated microbeads, and ERK1/2 MAPK activation was determined by immunoblotting with phosphospecific anti-ERK1/2 antibodies. Antibodies recognizing total ERK1/2 were used to verify equal protein loading. Data represent mean values ± SD of separate experiments ( n = 3); * p
    Rabbit P2y1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit p2y1/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit p2y1 - by Bioz Stars, 2022-01
    92/100 stars
      Buy from Supplier

    94
    Alomone Labs anti p2y1 receptor antibody
    <t>P2Y1</t> receptors promote IL-2 expression in response to TCR/CD28 stimulation. a CD4 T cells were treated for 10 min with the indicated concentrations of the P2Y1 antagonist MRS2279 and stimulated for 5 min with anti-CD3/CD28 antibody-coated microbeads, and ERK1/2 MAPK activation was determined by immunoblotting with phosphospecific anti-ERK1/2 antibodies. Antibodies recognizing total ERK1/2 were used to verify equal protein loading. Data represent mean values ± SD of separate experiments ( n = 3); * p
    Anti P2y1 Receptor Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti p2y1 receptor antibody/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti p2y1 receptor antibody - by Bioz Stars, 2022-01
    94/100 stars
      Buy from Supplier

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    P2Y1 receptors promote IL-2 expression in response to TCR/CD28 stimulation. a CD4 T cells were treated for 10 min with the indicated concentrations of the P2Y1 antagonist MRS2279 and stimulated for 5 min with anti-CD3/CD28 antibody-coated microbeads, and ERK1/2 MAPK activation was determined by immunoblotting with phosphospecific anti-ERK1/2 antibodies. Antibodies recognizing total ERK1/2 were used to verify equal protein loading. Data represent mean values ± SD of separate experiments ( n = 3); * p

    Journal: Purinergic Signalling

    Article Title: Autocrine stimulation of P2Y1 receptors is part of the purinergic signaling mechanism that regulates T cell activation

    doi: 10.1007/s11302-019-09653-6

    Figure Lengend Snippet: P2Y1 receptors promote IL-2 expression in response to TCR/CD28 stimulation. a CD4 T cells were treated for 10 min with the indicated concentrations of the P2Y1 antagonist MRS2279 and stimulated for 5 min with anti-CD3/CD28 antibody-coated microbeads, and ERK1/2 MAPK activation was determined by immunoblotting with phosphospecific anti-ERK1/2 antibodies. Antibodies recognizing total ERK1/2 were used to verify equal protein loading. Data represent mean values ± SD of separate experiments ( n = 3); * p

    Article Snippet: Rabbit P2Y1 (Cat. # APR-021) and P2Y12 (Cat. # APR-020) antibodies recognizing extracellular epitopes of human P2Y1 and P2Y12 receptors, respectively, were purchased from Alomone Labs (Jerusalem, Israel).

    Techniques: Expressing, Activation Assay

    Proposed mechanisms by which P2X4 and P2Y1 receptors synergize to regulate T cells. Stimulation of their T cells via T cell receptor (TCR) and CD28 coreceptors triggers mitochondrial ATP production and ATP release through pannexin-1 channels (Panx1). The released ATP activates P2X4 receptors that promote Ca 2+ influx. Hydrolysis of extracellular ATP by ectonucleotide triphosphate diphosphohydrolases (ENTPD) generates ADP that activates P2Y1 receptors that contribute to the increase in intracellular Ca 2+ concentrations. Increased mitochondrial activity, P2X4 and P2Y1 receptor Ca2+ signaling increases ATP release and fuel an autocrine feed-forward signaling mechanism that enhances T cell activation, and subsequent downstream signaling pathways that culminate in IL-2 transcription and effector functions involved in inflammation and host defense

    Journal: Purinergic Signalling

    Article Title: Autocrine stimulation of P2Y1 receptors is part of the purinergic signaling mechanism that regulates T cell activation

    doi: 10.1007/s11302-019-09653-6

    Figure Lengend Snippet: Proposed mechanisms by which P2X4 and P2Y1 receptors synergize to regulate T cells. Stimulation of their T cells via T cell receptor (TCR) and CD28 coreceptors triggers mitochondrial ATP production and ATP release through pannexin-1 channels (Panx1). The released ATP activates P2X4 receptors that promote Ca 2+ influx. Hydrolysis of extracellular ATP by ectonucleotide triphosphate diphosphohydrolases (ENTPD) generates ADP that activates P2Y1 receptors that contribute to the increase in intracellular Ca 2+ concentrations. Increased mitochondrial activity, P2X4 and P2Y1 receptor Ca2+ signaling increases ATP release and fuel an autocrine feed-forward signaling mechanism that enhances T cell activation, and subsequent downstream signaling pathways that culminate in IL-2 transcription and effector functions involved in inflammation and host defense

    Article Snippet: Rabbit P2Y1 (Cat. # APR-021) and P2Y12 (Cat. # APR-020) antibodies recognizing extracellular epitopes of human P2Y1 and P2Y12 receptors, respectively, were purchased from Alomone Labs (Jerusalem, Israel).

    Techniques: Activity Assay, Activation Assay

    CD4 T cells express ATP-selective P2X and ADP-selective P2Y receptors. a P2 receptor mRNA levels in purified CD4 T cells were determined with qPCR using CD4 T cells isolated from healthy human subjects or Jurkat T cells (mean ± SD, n = 3; n.d., not detected). b Total protein expression of P2Y1 and P2Y12 receptors in human CD4 T cells before and after TCR/CD28 stimulation. The results shown are representative of n = 3 experiments with cells from different subjects. c – d Cell surface expression of P2Y1 and P2Y12 receptors on human CD4 T cells was assessed with flow cytometry before or 72 h after TCR/CD28 stimulation. Representative histograms ( c ) and summarized data ( d ) of unstimulated (n = 5) or stimulated (n = 4) cell preparations with cells from different donors are shown (* p

    Journal: Purinergic Signalling

    Article Title: Autocrine stimulation of P2Y1 receptors is part of the purinergic signaling mechanism that regulates T cell activation

    doi: 10.1007/s11302-019-09653-6

    Figure Lengend Snippet: CD4 T cells express ATP-selective P2X and ADP-selective P2Y receptors. a P2 receptor mRNA levels in purified CD4 T cells were determined with qPCR using CD4 T cells isolated from healthy human subjects or Jurkat T cells (mean ± SD, n = 3; n.d., not detected). b Total protein expression of P2Y1 and P2Y12 receptors in human CD4 T cells before and after TCR/CD28 stimulation. The results shown are representative of n = 3 experiments with cells from different subjects. c – d Cell surface expression of P2Y1 and P2Y12 receptors on human CD4 T cells was assessed with flow cytometry before or 72 h after TCR/CD28 stimulation. Representative histograms ( c ) and summarized data ( d ) of unstimulated (n = 5) or stimulated (n = 4) cell preparations with cells from different donors are shown (* p

    Article Snippet: Rabbit P2Y1 (Cat. # APR-021) and P2Y12 (Cat. # APR-020) antibodies recognizing extracellular epitopes of human P2Y1 and P2Y12 receptors, respectively, were purchased from Alomone Labs (Jerusalem, Israel).

    Techniques: Purification, Real-time Polymerase Chain Reaction, Isolation, Expressing, Flow Cytometry, Cytometry

    P2Y1 and P2X4 receptors activate mitochondria in response to TCR/CD28 stimulation. a Jurkat cells expressing the mitochondrial Ca 2+ indicator mito-CAR-GECO1 were pretreated for 10 min with specific antagonists of P2X4 (5-BDBD, 10 μM) or P2Y1 (MRS2279, 10 μM) receptors or with the general P2 receptor antagonist suramin (100 μM). Then, cells were stimulated by TCR/CD28 cross-linking, and changes in mitochondrial Ca 2+ uptake were recorded over time using fluorescence microscopy. Traces of individual cells are shown in gray, and the averages of all cells acquired ( n = 30–80) are shown in red. Data shown are representative of independent experiments ( n ≥ 3). b Averaged mean fluorescence values (+ SEM) of cells ( n = 30-80) from different experiments ( n ≥ 3 experiments). c Peak mitochondrial Ca 2+ levels following TCR/CD28 stimulation; mean ± SEM; * p

    Journal: Purinergic Signalling

    Article Title: Autocrine stimulation of P2Y1 receptors is part of the purinergic signaling mechanism that regulates T cell activation

    doi: 10.1007/s11302-019-09653-6

    Figure Lengend Snippet: P2Y1 and P2X4 receptors activate mitochondria in response to TCR/CD28 stimulation. a Jurkat cells expressing the mitochondrial Ca 2+ indicator mito-CAR-GECO1 were pretreated for 10 min with specific antagonists of P2X4 (5-BDBD, 10 μM) or P2Y1 (MRS2279, 10 μM) receptors or with the general P2 receptor antagonist suramin (100 μM). Then, cells were stimulated by TCR/CD28 cross-linking, and changes in mitochondrial Ca 2+ uptake were recorded over time using fluorescence microscopy. Traces of individual cells are shown in gray, and the averages of all cells acquired ( n = 30–80) are shown in red. Data shown are representative of independent experiments ( n ≥ 3). b Averaged mean fluorescence values (+ SEM) of cells ( n = 30-80) from different experiments ( n ≥ 3 experiments). c Peak mitochondrial Ca 2+ levels following TCR/CD28 stimulation; mean ± SEM; * p

    Article Snippet: Rabbit P2Y1 (Cat. # APR-021) and P2Y12 (Cat. # APR-020) antibodies recognizing extracellular epitopes of human P2Y1 and P2Y12 receptors, respectively, were purchased from Alomone Labs (Jerusalem, Israel).

    Techniques: Expressing, Fluorescence, Microscopy

    P2Y1 and P2X4 receptors regulate cytosolic Ca 2+ signaling in response to TCR/CD28 stimulation. a Primary human CD4 T cells were loaded with the cytosolic Ca 2+ probe Fluo-4 AM, pretreated for 10 min with specific antagonists of P2X4 (5-BDBD, 10 μM) or P2Y1 (MRS2279, 10 μM) receptors or with the general P2 receptor antagonist suramin (100 μM). Then, cells were stimulated by TCR/CD28 cross-linking, and changes in cytosolic Ca 2+ levels were recorded over time using live-cell fluorescence microscopy. Traces of individual cells are shown in gray, and averages of all cells studied ( n = 120–350) are shown in red. Data shown are representative of different experiments ( n ≥ 3) with cells from different donors. b Averaged mean fluorescence values (+ SEM) of cells from at least three different donors ( n = 120-350 per experiment). c Peak Ca 2+ levels following TCR/CD28 stimulation; mean ± SEM; * p

    Journal: Purinergic Signalling

    Article Title: Autocrine stimulation of P2Y1 receptors is part of the purinergic signaling mechanism that regulates T cell activation

    doi: 10.1007/s11302-019-09653-6

    Figure Lengend Snippet: P2Y1 and P2X4 receptors regulate cytosolic Ca 2+ signaling in response to TCR/CD28 stimulation. a Primary human CD4 T cells were loaded with the cytosolic Ca 2+ probe Fluo-4 AM, pretreated for 10 min with specific antagonists of P2X4 (5-BDBD, 10 μM) or P2Y1 (MRS2279, 10 μM) receptors or with the general P2 receptor antagonist suramin (100 μM). Then, cells were stimulated by TCR/CD28 cross-linking, and changes in cytosolic Ca 2+ levels were recorded over time using live-cell fluorescence microscopy. Traces of individual cells are shown in gray, and averages of all cells studied ( n = 120–350) are shown in red. Data shown are representative of different experiments ( n ≥ 3) with cells from different donors. b Averaged mean fluorescence values (+ SEM) of cells from at least three different donors ( n = 120-350 per experiment). c Peak Ca 2+ levels following TCR/CD28 stimulation; mean ± SEM; * p

    Article Snippet: Rabbit P2Y1 (Cat. # APR-021) and P2Y12 (Cat. # APR-020) antibodies recognizing extracellular epitopes of human P2Y1 and P2Y12 receptors, respectively, were purchased from Alomone Labs (Jerusalem, Israel).

    Techniques: Fluorescence, Microscopy