anti p2ry12  (Alomone Labs)


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    Structured Review

    Alomone Labs anti p2ry12
    Characterizations of induced microglia-like cells (hiMG) and synaptosomes isolated from neural progenitor cell (NPC)-derived neural cultures. ( a ) Representative phase-contrast image of hiMG. ( b – d ) Immunocytochemistry of hiMG stained with antibodies to ( b ) <t>P2RY12,</t> ( c ) CX3CR1, ( d ) merged P2RY12 (red), CXCR1 (green) and nuclear Hoechst (blue). Scale bar=60 μm. ( e ) Heat map based on hierarchical clustering of hiMG, monocyte-derived macrophages (MΦ), primary fetal microglia (MGf), SV40 immortalized primary microglia (imMG) and microglia isolated from adult post-mortem brain tissue (pmMGa). Results were log2-transformed, normalized to a set of housekeeping genes ( ACTB, b2M, IDHA and PGK1 ), centered, and populations were clustered using an agglomerative algorithm. The data are presented as duplicates representative of two subjects for hiMG, MΦ, MGf and pmMGa, and one subject for imMG. ( f ) Hierarchical cluster analysis (median linking and correlation as distance metric) of the data in ( e ) using the R package pvclust. Uncertainty in clustering is assessed via multiscale bootstrap resampling (nboot=10,000) and Approximately Unbiased (AU) probability value as well as ordinary Bootstrap Probability (BP) value is provided for each cluster. Clusters with a significance level of
    Anti P2ry12, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti p2ry12/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti p2ry12 - by Bioz Stars, 2022-01
    92/100 stars

    Images

    1) Product Images from "Patient-specific models of microglia-mediated engulfment of synapses and neural progenitors"

    Article Title: Patient-specific models of microglia-mediated engulfment of synapses and neural progenitors

    Journal: Molecular Psychiatry

    doi: 10.1038/mp.2016.220

    Characterizations of induced microglia-like cells (hiMG) and synaptosomes isolated from neural progenitor cell (NPC)-derived neural cultures. ( a ) Representative phase-contrast image of hiMG. ( b – d ) Immunocytochemistry of hiMG stained with antibodies to ( b ) P2RY12, ( c ) CX3CR1, ( d ) merged P2RY12 (red), CXCR1 (green) and nuclear Hoechst (blue). Scale bar=60 μm. ( e ) Heat map based on hierarchical clustering of hiMG, monocyte-derived macrophages (MΦ), primary fetal microglia (MGf), SV40 immortalized primary microglia (imMG) and microglia isolated from adult post-mortem brain tissue (pmMGa). Results were log2-transformed, normalized to a set of housekeeping genes ( ACTB, b2M, IDHA and PGK1 ), centered, and populations were clustered using an agglomerative algorithm. The data are presented as duplicates representative of two subjects for hiMG, MΦ, MGf and pmMGa, and one subject for imMG. ( f ) Hierarchical cluster analysis (median linking and correlation as distance metric) of the data in ( e ) using the R package pvclust. Uncertainty in clustering is assessed via multiscale bootstrap resampling (nboot=10,000) and Approximately Unbiased (AU) probability value as well as ordinary Bootstrap Probability (BP) value is provided for each cluster. Clusters with a significance level of
    Figure Legend Snippet: Characterizations of induced microglia-like cells (hiMG) and synaptosomes isolated from neural progenitor cell (NPC)-derived neural cultures. ( a ) Representative phase-contrast image of hiMG. ( b – d ) Immunocytochemistry of hiMG stained with antibodies to ( b ) P2RY12, ( c ) CX3CR1, ( d ) merged P2RY12 (red), CXCR1 (green) and nuclear Hoechst (blue). Scale bar=60 μm. ( e ) Heat map based on hierarchical clustering of hiMG, monocyte-derived macrophages (MΦ), primary fetal microglia (MGf), SV40 immortalized primary microglia (imMG) and microglia isolated from adult post-mortem brain tissue (pmMGa). Results were log2-transformed, normalized to a set of housekeeping genes ( ACTB, b2M, IDHA and PGK1 ), centered, and populations were clustered using an agglomerative algorithm. The data are presented as duplicates representative of two subjects for hiMG, MΦ, MGf and pmMGa, and one subject for imMG. ( f ) Hierarchical cluster analysis (median linking and correlation as distance metric) of the data in ( e ) using the R package pvclust. Uncertainty in clustering is assessed via multiscale bootstrap resampling (nboot=10,000) and Approximately Unbiased (AU) probability value as well as ordinary Bootstrap Probability (BP) value is provided for each cluster. Clusters with a significance level of

    Techniques Used: Isolation, Derivative Assay, Immunocytochemistry, Staining, Transformation Assay

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    Alomone Labs anti p2ry12
    Characterizations of induced microglia-like cells (hiMG) and synaptosomes isolated from neural progenitor cell (NPC)-derived neural cultures. ( a ) Representative phase-contrast image of hiMG. ( b – d ) Immunocytochemistry of hiMG stained with antibodies to ( b ) <t>P2RY12,</t> ( c ) CX3CR1, ( d ) merged P2RY12 (red), CXCR1 (green) and nuclear Hoechst (blue). Scale bar=60 μm. ( e ) Heat map based on hierarchical clustering of hiMG, monocyte-derived macrophages (MΦ), primary fetal microglia (MGf), SV40 immortalized primary microglia (imMG) and microglia isolated from adult post-mortem brain tissue (pmMGa). Results were log2-transformed, normalized to a set of housekeeping genes ( ACTB, b2M, IDHA and PGK1 ), centered, and populations were clustered using an agglomerative algorithm. The data are presented as duplicates representative of two subjects for hiMG, MΦ, MGf and pmMGa, and one subject for imMG. ( f ) Hierarchical cluster analysis (median linking and correlation as distance metric) of the data in ( e ) using the R package pvclust. Uncertainty in clustering is assessed via multiscale bootstrap resampling (nboot=10,000) and Approximately Unbiased (AU) probability value as well as ordinary Bootstrap Probability (BP) value is provided for each cluster. Clusters with a significance level of
    Anti P2ry12, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti p2ry12/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti p2ry12 - by Bioz Stars, 2022-01
    92/100 stars
      Buy from Supplier

    94
    Alomone Labs anti p2y12
    Characterizations of induced microglia-like cells (hiMG) and synaptosomes isolated from neural progenitor cell (NPC)-derived neural cultures. ( a ) Representative phase-contrast image of hiMG. ( b – d ) Immunocytochemistry of hiMG stained with antibodies to ( b ) <t>P2RY12,</t> ( c ) CX3CR1, ( d ) merged P2RY12 (red), CXCR1 (green) and nuclear Hoechst (blue). Scale bar=60 μm. ( e ) Heat map based on hierarchical clustering of hiMG, monocyte-derived macrophages (MΦ), primary fetal microglia (MGf), SV40 immortalized primary microglia (imMG) and microglia isolated from adult post-mortem brain tissue (pmMGa). Results were log2-transformed, normalized to a set of housekeeping genes ( ACTB, b2M, IDHA and PGK1 ), centered, and populations were clustered using an agglomerative algorithm. The data are presented as duplicates representative of two subjects for hiMG, MΦ, MGf and pmMGa, and one subject for imMG. ( f ) Hierarchical cluster analysis (median linking and correlation as distance metric) of the data in ( e ) using the R package pvclust. Uncertainty in clustering is assessed via multiscale bootstrap resampling (nboot=10,000) and Approximately Unbiased (AU) probability value as well as ordinary Bootstrap Probability (BP) value is provided for each cluster. Clusters with a significance level of
    Anti P2y12, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti p2y12/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti p2y12 - by Bioz Stars, 2022-01
    94/100 stars
      Buy from Supplier

    Image Search Results


    Characterizations of induced microglia-like cells (hiMG) and synaptosomes isolated from neural progenitor cell (NPC)-derived neural cultures. ( a ) Representative phase-contrast image of hiMG. ( b – d ) Immunocytochemistry of hiMG stained with antibodies to ( b ) P2RY12, ( c ) CX3CR1, ( d ) merged P2RY12 (red), CXCR1 (green) and nuclear Hoechst (blue). Scale bar=60 μm. ( e ) Heat map based on hierarchical clustering of hiMG, monocyte-derived macrophages (MΦ), primary fetal microglia (MGf), SV40 immortalized primary microglia (imMG) and microglia isolated from adult post-mortem brain tissue (pmMGa). Results were log2-transformed, normalized to a set of housekeeping genes ( ACTB, b2M, IDHA and PGK1 ), centered, and populations were clustered using an agglomerative algorithm. The data are presented as duplicates representative of two subjects for hiMG, MΦ, MGf and pmMGa, and one subject for imMG. ( f ) Hierarchical cluster analysis (median linking and correlation as distance metric) of the data in ( e ) using the R package pvclust. Uncertainty in clustering is assessed via multiscale bootstrap resampling (nboot=10,000) and Approximately Unbiased (AU) probability value as well as ordinary Bootstrap Probability (BP) value is provided for each cluster. Clusters with a significance level of

    Journal: Molecular Psychiatry

    Article Title: Patient-specific models of microglia-mediated engulfment of synapses and neural progenitors

    doi: 10.1038/mp.2016.220

    Figure Lengend Snippet: Characterizations of induced microglia-like cells (hiMG) and synaptosomes isolated from neural progenitor cell (NPC)-derived neural cultures. ( a ) Representative phase-contrast image of hiMG. ( b – d ) Immunocytochemistry of hiMG stained with antibodies to ( b ) P2RY12, ( c ) CX3CR1, ( d ) merged P2RY12 (red), CXCR1 (green) and nuclear Hoechst (blue). Scale bar=60 μm. ( e ) Heat map based on hierarchical clustering of hiMG, monocyte-derived macrophages (MΦ), primary fetal microglia (MGf), SV40 immortalized primary microglia (imMG) and microglia isolated from adult post-mortem brain tissue (pmMGa). Results were log2-transformed, normalized to a set of housekeeping genes ( ACTB, b2M, IDHA and PGK1 ), centered, and populations were clustered using an agglomerative algorithm. The data are presented as duplicates representative of two subjects for hiMG, MΦ, MGf and pmMGa, and one subject for imMG. ( f ) Hierarchical cluster analysis (median linking and correlation as distance metric) of the data in ( e ) using the R package pvclust. Uncertainty in clustering is assessed via multiscale bootstrap resampling (nboot=10,000) and Approximately Unbiased (AU) probability value as well as ordinary Bootstrap Probability (BP) value is provided for each cluster. Clusters with a significance level of

    Article Snippet: Primary antibodies were diluted in PBS with 1% FBS as follows: CX3CR1 (Novus Biologicals; #H00001524-B01P; 1:500), Anti-P2RY12 (Alomone Labs, Jerusalem, Israel; #APR-020; 1:100), C4 (Abcam; #173577; 1:250), CD11b (Abcam; #133357; 1:250) and PSD-95 (EMD Millipore; #MABN68; 1:500), IBA1 (Abcam ab15690; 1:200), TMEM119 (Abcam; ab185337; 1:200), PU.1/Spi1 (Abcam; ab88082; 1:200), MAP2 (Abcam; ab92434, 1:1000), SMI312 (Biolegend; 837904, 1:1000) and βIII-Tubulin (Millipore; MAB5564; 1:1500).

    Techniques: Isolation, Derivative Assay, Immunocytochemistry, Staining, Transformation Assay