p2y12  (Alomone Labs)

Journal: Purinergic Signalling

Article Title: Using antibodies against P2Y and P2X receptors in purinergic signaling research

doi: 10.1007/s11302-011-9278-z

Figure Lengend Snippet: Expression of P2Y receptors in osteoblasts. Using flow cytometry analysis, P2Y 1 , P2Y 2 , P2Y 4 , P2Y 6 , P2Y 11 , P2Y 12 , and P2Y 13 receptors were detected in osteoblasts using their respective Alomone Labs antibodies ( open histograms ). Incubating the antibody with the control peptide antigen was used as a negative control ( filled histograms ). Adapted from reference 41 with permission of Elsevier

Article Snippet: Using a panel of Alomone Labs anti-P2Y receptor antibodies, it was demonstrated that P2Y1 , P2Y2 , P2Y4 , P2Y6 , P2Y11 , and P2Y13 are all expressed by myofibroblasts and that all except for P2Y13 are also functional, as demonstrated by the use of pharmacological agents [ ].

Techniques: Expressing, Flow Cytometry, Cytometry, Negative Control

Journal: Purinergic Signalling

Article Title: Using antibodies against P2Y and P2X receptors in purinergic signaling research

doi: 10.1007/s11302-011-9278-z

Figure Lengend Snippet: Expression of P2Y receptors in Müller cells of the adult rat and mouse retinal slices. Immunohistochemical staining was accomplished using anti-P2Y 2 receptor (#APR-010) and anti-P2Y 6 receptor (#APR-011) antibodies. a No distinct immunoreactivity for the P2Y 2 receptor ( red ) was observed in Müller cells. However, ganglion cells ( asterisk ) and structures within the inner plexiform layer ( IPL ), inner nuclear layer ( INL ), and outer plexiform layer ( OPL ) display faint staining. b P2Y 4 receptor ( red ) is highly expressed in Müller cell end-feet ( arrows ), as detected using anti-P2Y 4 receptor antibody (#APR-006). Additionally, a band of globular structures, probably representing synapse of bipolar cells in the ON-sublamina ( ON ) of the IPL, is stained for this receptor. c The staining pattern of the P2Y 6 receptor ( red ) is similar to that of P2Y 2 . The insets in ( b ) and ( c ) show the ganglion cell layer ( GCL ) and the INL, respectively, at higher magnification. ONL outer nuclear layer, OFF OFF-sublamina of the IPL, PRS photoreceptor segments. Scale bars , 20 μm; inset scale bars , 10 μm. d Immunohistochemical staining was carried out using anti-P2Y 4 receptor antibody. The slices were co-stained against the glial cell marker cellular retinaldehyde-binding protein ( CRALBP ); co-labeling yielded a yellow-orange merge signal. Arrows , Müller cell end-feet; arrowheads , Müller cell somata. GCL ganglion cell layer, IPL inner plexiform layer, NFL nerve fiber layer, ONL outer nuclear layer, PRS , photoreceptor segments. Scale bars , 20 μm. a–c Adapted from reference 12 with permission of John Wiley and Sons. d Adapted from reference 13 with permission of Wiley-Blackwell

Article Snippet: Using a panel of Alomone Labs anti-P2Y receptor antibodies, it was demonstrated that P2Y1 , P2Y2 , P2Y4 , P2Y6 , P2Y11 , and P2Y13 are all expressed by myofibroblasts and that all except for P2Y13 are also functional, as demonstrated by the use of pharmacological agents [ ].

Techniques: Expressing, Immunohistochemistry, Staining, Marker, Binding Assay, Labeling

Journal: Purinergic Signalling

Article Title: Using antibodies against P2Y and P2X receptors in purinergic signaling research

doi: 10.1007/s11302-011-9278-z

Figure Lengend Snippet: Differential expression of P2Y receptors in glial following retinal detachment. Immunohistochemical staining of control retinas and from detached areas of porcine retinas 7 days after surgery was done using anti-P2Y 1 receptor (#APR-009), anti-P2Y 2 receptor (#APR-010), and anti-P2Y 4 receptor (#APR-006) antibodies ( red ). Immunoreactivities for P2Y 1 , P2Y 2 , and P2Y 4 receptor proteins ( red ) coincide with that of intermediate filament constituents vimentin ( green and yellow for merging signal). Arrows , vimentin-positive fibers in the outer retina that were also stained for P2Y 2 protein. GCL ganglion cell layer, INL inner nuclear layer, IPL inner plexiform layer, NFL nerve fiber layer, ONL outer nuclear layer. Scale bars , 20 μm. Adapted from reference 15 with permission of Association for Research in Vision and Ophthalmology

Article Snippet: Using a panel of Alomone Labs anti-P2Y receptor antibodies, it was demonstrated that P2Y1 , P2Y2 , P2Y4 , P2Y6 , P2Y11 , and P2Y13 are all expressed by myofibroblasts and that all except for P2Y13 are also functional, as demonstrated by the use of pharmacological agents [ ].

Techniques: Expressing, Immunohistochemistry, Staining

Journal: Purinergic Signalling

Article Title: Using antibodies against P2Y and P2X receptors in purinergic signaling research

doi: 10.1007/s11302-011-9278-z

Figure Lengend Snippet: Expression of P2Y receptors in rat duodenal epithelium. Immunohistochemical staining was done using anti-P2Y 1 receptor antibody (#APR-009), anti-P2Y 2 receptor antibody (#APR-010), and anti-P2Y 6 receptor antibody (#APR-011). a P2Y 1 is expressed along the brush border membranes of villous cells. P2Y 2 and P2Y 4 receptors are not expressed ( b , c ). d P2Y 6 receptor is diffusely expressed in the villous cells and interstitium. Adapted from reference 31 with permission of John Wiley and Sons

Article Snippet: Using a panel of Alomone Labs anti-P2Y receptor antibodies, it was demonstrated that P2Y1 , P2Y2 , P2Y4 , P2Y6 , P2Y11 , and P2Y13 are all expressed by myofibroblasts and that all except for P2Y13 are also functional, as demonstrated by the use of pharmacological agents [ ].

Techniques: Expressing, Immunohistochemistry, Staining

Journal: Purinergic Signalling

Article Title: Using antibodies against P2Y and P2X receptors in purinergic signaling research

doi: 10.1007/s11302-011-9278-z

Figure Lengend Snippet: Expression of P2Y receptors in rat distal colon. All P2Y immunohistochemical staining was done using Alomone Labs anti-P2Y receptor antibodies. a Double immunolabeling reveals that P2Y 1 is expressed in enteric smooth muscle cells and S100 in enteric glial cells. b In the myenteric plexus, P2Y 2 is detected in neurons. c P2Y 4 is detected in enteric glial cells. d P2Y 6 is expressed in vascular and enteric smooth muscle cells. e P2Y 11 is detected in enteric glial cells and P2Y 12 is expressed in enteric nerves ( f ). Adapted from reference 30 with permission of Elsevier

Article Snippet: Using a panel of Alomone Labs anti-P2Y receptor antibodies, it was demonstrated that P2Y1 , P2Y2 , P2Y4 , P2Y6 , P2Y11 , and P2Y13 are all expressed by myofibroblasts and that all except for P2Y13 are also functional, as demonstrated by the use of pharmacological agents [ ].

Techniques: Expressing, Immunohistochemistry, Staining, Immunolabeling

Journal: Purinergic Signalling

Article Title: Involvement of P2X and P2Y receptors in microglial activation in vivo

doi: 10.1007/s11302-007-9082-y

Figure Lengend Snippet: Confocal images of examples of double immunofluorescence labeling to characterize the colocalization of P2X 1,2,4,7 and P2Y 1,6,12 receptor subtypes on a–f OX42- or g–o GSA-B4-labeled microglial cells in the NAc of rats 4 days after stab-wound injury ( thin arrow process-bearing microglial cell; thick arrow activated microglial cell) ( scale bars : a, b = 20 μm; c, d = 100 μm; e–i = 20 μm; j, k = 100 μm; l, m = 20 μm; n, o = 50 μm)

Article Snippet: Double-immunofluorescence studies After washing with TBS (0.05 M; pH 7.6) and blocking with normal goat serum (1% NGS) in TBS, slices were incubated in an antibody mixture of mouse anti-OX 42 (CD11b; 1:100) and rabbit anti-P2X receptor antibodies (P2X1 1:400; P2X2 1:400; P2X4 1:400; P2X5 1:400; P2X6 1:400; P2X7 1:1,000) or P2Y receptor antibodies [P2Y1 1:400; P2Y2 1:1,000; P2Y4 1:1,000; P2Y12 1:400 (Alomone Labs); P2Y1 : 1:1,500 (GlaxoSmithKline); P2Y6 1:200 (Santa Cruz Biotechnology, Inc.)] with 0.1% Triton X-100 in 1% NGS in TBS for 48 h at 4°C.

Techniques: Immunofluorescence, Labeling