Journal: Purinergic Signalling
Article Title: Using antibodies against P2Y and P2X receptors in purinergic signaling research
Figure Lengend Snippet: Expression of P2Y receptors in Müller cells of the adult rat and mouse retinal slices. Immunohistochemical staining was accomplished using anti-P2Y 2 receptor (#APR-010) and anti-P2Y 6 receptor (#APR-011) antibodies. a No distinct immunoreactivity for the P2Y 2 receptor ( red ) was observed in Müller cells. However, ganglion cells ( asterisk ) and structures within the inner plexiform layer ( IPL ), inner nuclear layer ( INL ), and outer plexiform layer ( OPL ) display faint staining. b P2Y 4 receptor ( red ) is highly expressed in Müller cell end-feet ( arrows ), as detected using anti-P2Y 4 receptor antibody (#APR-006). Additionally, a band of globular structures, probably representing synapse of bipolar cells in the ON-sublamina ( ON ) of the IPL, is stained for this receptor. c The staining pattern of the P2Y 6 receptor ( red ) is similar to that of P2Y 2 . The insets in ( b ) and ( c ) show the ganglion cell layer ( GCL ) and the INL, respectively, at higher magnification. ONL outer nuclear layer, OFF OFF-sublamina of the IPL, PRS photoreceptor segments. Scale bars , 20 μm; inset scale bars , 10 μm. d Immunohistochemical staining was carried out using anti-P2Y 4 receptor antibody. The slices were co-stained against the glial cell marker cellular retinaldehyde-binding protein ( CRALBP ); co-labeling yielded a yellow-orange merge signal. Arrows , Müller cell end-feet; arrowheads , Müller cell somata. GCL ganglion cell layer, IPL inner plexiform layer, NFL nerve fiber layer, ONL outer nuclear layer, PRS , photoreceptor segments. Scale bars , 20 μm. a–c Adapted from reference 12 with permission of John Wiley and Sons. d Adapted from reference 13 with permission of Wiley-Blackwell
Article Snippet: Using a panel of Alomone Labs anti-P2Y receptor antibodies, it was demonstrated that P2Y1 , P2Y2 , P2Y4 , P2Y6 , P2Y11 , and P2Y13 are all expressed by myofibroblasts and that all except for P2Y13 are also functional, as demonstrated by the use of pharmacological agents [ ].
Techniques: Expressing, Immunohistochemistry, Staining, Marker, Binding Assay, Labeling